Click here to switch to the map view.

The map label for this gene is paaF [C]

Identifier: 94309786

GI number: 94309786

Start: 920208

End: 921026

Strand: Direct

Name: paaF [C]

Synonym: Rmet_0841

Alternate gene names: 94309786

Gene position: 920208-921026 (Clockwise)

Preceding gene: 94309784

Following gene: 94309787

Centisome position: 23.43

GC content: 64.1

Gene sequence:

>819_bases
ATGCCTTACACCACCATCGAAATCGAACAGCGCGACGCCGTGCGTCGCGTCTGGCTGGCGCGCGAAAGCGCCCGTAATGC
GCAAAGCCAGCGGATGCTGGACGAACTGGATCACGCGATGTCCGAAGCAGCCACCGACGACTCGGTGCGCGTGGTGGTGA
TCGGCGGTCGCGGTGCGCACTTTTCCGCCGGGCACGATCTCAAGGAAGCGCAGGAGAAACGCGCCAACTTCACGGTGGAG
GAGCGCTGGGCGTACGAAAGCGAGCGGTACTTCAACTACTGCATGCGCATCTGGGATTTCCCAAAGCCGACCATCGCGCA
AGTGCAGGGCGCATGCGTCTCCGGCGGCTTCATGATCGCCAATATGTGCGATCTGGTGGTGGCTTCCGAGTCGGCGTACT
TCTCCGATCCGGTCGGCCATTCGCTCGGCGCGGCGGCCACCGAGGTGCTGATTCATCCGTGGGTCATGGGGCTCAGGAAA
GCCAAGGAAATGCTCTTCACCGGGGATCGGATCGATGCGGCTGAAGCGTTGCGCATCGGCATGGTGAATCGGGTGGTCGC
GGAAGGGGAACTGGAAGCGCAAACCGATGCGCTGGCCGCCCGTATCGCCAAGGCGCCGCCGTTCGGCCTGCATCTGATCA
AGCGCTCCCTGAACCGCACGCTGGACGCGCAGGGTCTTCGCTCGGCGCTGTCGGCGCACTTCGACACCCATCAGCTCTCT
CATCTCGGGGAGGGGTTCCTGCGCGCGCGGGATGCCGGCCTTGCCAAGGCCATCGTCAAGAATGTCGCCGTGGACGCCGA
CAAGCAAGGAACCCGCTGA

Upstream 100 bases:

>100_bases
AGTTGCTTGTGTTTGTATTGTGAAAATACCTGCTTTTACACTCGATTGGAATCACTCGACACAGCCAAGACGTCAAACCA
CATCGAACAGAGGAGCCGGG

Downstream 100 bases:

>100_bases
CATGGCGCACATGCTCGAACCCCTGTTGAATCCCGCCTCCATCGCGATCATCGGCGCCAGCGACAATCCGGGCCGCATCG
GCGGTATGCCGATAGACCTG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MPYTTIEIEQRDAVRRVWLARESARNAQSQRMLDELDHAMSEAATDDSVRVVVIGGRGAHFSAGHDLKEAQEKRANFTVE
ERWAYESERYFNYCMRIWDFPKPTIAQVQGACVSGGFMIANMCDLVVASESAYFSDPVGHSLGAAATEVLIHPWVMGLRK
AKEMLFTGDRIDAAEALRIGMVNRVVAEGELEAQTDALAARIAKAPPFGLHLIKRSLNRTLDAQGLRSALSAHFDTHQLS
HLGEGFLRARDAGLAKAIVKNVAVDADKQGTR

Sequences:

>Translated_272_residues
MPYTTIEIEQRDAVRRVWLARESARNAQSQRMLDELDHAMSEAATDDSVRVVVIGGRGAHFSAGHDLKEAQEKRANFTVE
ERWAYESERYFNYCMRIWDFPKPTIAQVQGACVSGGFMIANMCDLVVASESAYFSDPVGHSLGAAATEVLIHPWVMGLRK
AKEMLFTGDRIDAAEALRIGMVNRVVAEGELEAQTDALAARIAKAPPFGLHLIKRSLNRTLDAQGLRSALSAHFDTHQLS
HLGEGFLRARDAGLAKAIVKNVAVDADKQGTR
>Mature_271_residues
PYTTIEIEQRDAVRRVWLARESARNAQSQRMLDELDHAMSEAATDDSVRVVVIGGRGAHFSAGHDLKEAQEKRANFTVEE
RWAYESERYFNYCMRIWDFPKPTIAQVQGACVSGGFMIANMCDLVVASESAYFSDPVGHSLGAAATEVLIHPWVMGLRKA
KEMLFTGDRIDAAEALRIGMVNRVVAEGELEAQTDALAARIAKAPPFGLHLIKRSLNRTLDAQGLRSALSAHFDTHQLSH
LGEGFLRARDAGLAKAIVKNVAVDADKQGTR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=199, Percent_Identity=34.6733668341709, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI194097323, Length=216, Percent_Identity=29.6296296296296, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI45643119, Length=225, Percent_Identity=28.8888888888889, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI260275230, Length=225, Percent_Identity=28.8888888888889, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI260274832, Length=225, Percent_Identity=28.8888888888889, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI25453481, Length=220, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI51538573, Length=220, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI4757966, Length=220, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI51538581, Length=220, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI221136756, Length=213, Percent_Identity=27.6995305164319, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI221136753, Length=213, Percent_Identity=27.6995305164319, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI221307494, Length=217, Percent_Identity=27.1889400921659, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI4757968, Length=197, Percent_Identity=27.9187817258883, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI49355787, Length=197, Percent_Identity=27.9187817258883, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI70995211, Length=189, Percent_Identity=30.6878306878307, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI116235487, Length=190, Percent_Identity=27.3684210526316, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787659, Length=220, Percent_Identity=32.7272727272727, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1788597, Length=218, Percent_Identity=27.9816513761468, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI221142681, Length=219, Percent_Identity=31.0502283105023, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI87082183, Length=232, Percent_Identity=28.448275862069, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787660, Length=220, Percent_Identity=29.0909090909091, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=223, Percent_Identity=33.6322869955157, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=221, Percent_Identity=28.9592760180996, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560910, Length=209, Percent_Identity=31.1004784688995, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=205, Percent_Identity=30.2439024390244, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=230, Percent_Identity=25.6521739130435, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17540714, Length=230, Percent_Identity=26.0869565217391, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=226, Percent_Identity=29.646017699115, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=219, Percent_Identity=26.4840182648402, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24650670, Length=212, Percent_Identity=34.4339622641509, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI20129971, Length=229, Percent_Identity=28.82096069869, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24653477, Length=229, Percent_Identity=28.82096069869, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24653139, Length=255, Percent_Identity=27.843137254902, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21357171, Length=209, Percent_Identity=29.1866028708134, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19920382, Length=219, Percent_Identity=28.7671232876712, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19922422, Length=250, Percent_Identity=24.8, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29994; Mature: 29863

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYTTIEIEQRDAVRRVWLARESARNAQSQRMLDELDHAMSEAATDDSVRVVVIGGRGAH
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
FSAGHDLKEAQEKRANFTVEERWAYESERYFNYCMRIWDFPKPTIAQVQGACVSGGFMIA
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHCCCCHHH
NMCDLVVASESAYFSDPVGHSLGAAATEVLIHPWVMGLRKAKEMLFTGDRIDAAEALRIG
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
MVNRVVAEGELEAQTDALAARIAKAPPFGLHLIKRSLNRTLDAQGLRSALSAHFDTHQLS
HHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHH
HLGEGFLRARDAGLAKAIVKNVAVDADKQGTR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PYTTIEIEQRDAVRRVWLARESARNAQSQRMLDELDHAMSEAATDDSVRVVVIGGRGAH
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
FSAGHDLKEAQEKRANFTVEERWAYESERYFNYCMRIWDFPKPTIAQVQGACVSGGFMIA
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHCCCCHHH
NMCDLVVASESAYFSDPVGHSLGAAATEVLIHPWVMGLRKAKEMLFTGDRIDAAEALRIG
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
MVNRVVAEGELEAQTDALAARIAKAPPFGLHLIKRSLNRTLDAQGLRSALSAHFDTHQLS
HHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHH
HLGEGFLRARDAGLAKAIVKNVAVDADKQGTR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA