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The map label for this gene is bcr [H]

Identifier: 94309722

GI number: 94309722

Start: 853446

End: 854702

Strand: Direct

Name: bcr [H]

Synonym: Rmet_0777

Alternate gene names: 94309722

Gene position: 853446-854702 (Clockwise)

Preceding gene: 94309721

Following gene: 94309731

Centisome position: 21.73

GC content: 65.63

Gene sequence:

>1257_bases
ATGAGTGAAGTGGATGTGCCTGGGGCAGTCGTAGCAGCGGGCGCGGAAGCGGGCCCCAGGCGCCCCAGCGTCGGCAGCAT
GGCGGCACTGACCGCTGGCTTGTCGATGCTGGGGCAGTTTGCTATTGCGACGTACCTGCCAGCGTTCGCGGCAATTGCGA
ACGCGCTTCATGCGAGCACTGCCGAGGTCCAGCAATCGCTGACGGCCTACCTGTTGCCGTTTGCATTGCTGCTGCCCTGG
CACGGTGCGATCTCGGACGCGATTGGCCGCCGCCGCATGATTCTTGCGGGCTGCGTGTTCTTTGTAGCCGGATCGTTGGT
CTGCGCCGGAGCGACCGGCATCGACATGTTCTACGTCGGGCGGGCACTGCAAGGCGTGGGGGCAGGGATCGGCGTCATCG
TCGGGCGCGCGATGGTGCGCGATACGTTCGACGGCATGCAGGCCCAGAAAGTCATGGCCCTGATCGCGATGATCTTCGCG
CTGGCGCCGGCCCTGGCGCCGATCTGCGGCGGCTGGCTGCTGGTCTGGATGGGCTGGCGCAGCATCTTCGTGTTCCTTGC
GCTGATCTCGGGGAGCCTGCTGGTTTCCTGCTGGATCTGGGTGCCGGAGACATTGCCGCCAGCGTGTCGGCATCCGATGC
ACCCAAAGGTGCTCGCGCGGGGCTACTTCGCCATGCTGTCGAGCCGAAGATTCATGTCGCTCGCGCTGGCCAATGCCGGT
GTCAACCTGGCGATCTACATCTACGTCTTCGCGGCGCCACGCTTTGTCTATCAGCATCTGGGGCTCGGCGCGCAGTCGTT
TGGCTGGCTGTTCATTCCGATCGTGGCGGGCATCATCGCGGGTGCGCTGGTGGCGCATCGCATTGCCGGCCGCACGACTC
CGGAGTCCAGCGTGAATCGGGGCCATGTCGTGATGATGGCGGCCGGCGTCTTCAATCTCGCGGTGGCCGCGCTGCACCTG
CCGCCGATGCCTTGGGCGCTGGTCGCATTGCCGGTCTTCGCGGCAGGAATGATGATGACGCAGCCCAGCCTGCAGCTGCT
GGCGCTGGATTGCTTTCCGGAGCGGAGGGGTCTGGCCTCCAGCGGCTACGTCACGGCGCAGCAGATCGGCAATTTGCTGT
CTTCCGCGTTGCTGGTGCCGCTGCTGCTGGACTCCACGATGAAGATGGCGCTCGGCATGGCCGGCTTGCAGATCGCAGGG
CTCGTGGCGTTCCACGTGGCCCTGCGTGGCCGGCGGGTGGGGGTTACATCAGAATGA

Upstream 100 bases:

>100_bases
GCGCATCGCGCACCTATGAGCGCGATCTGCAGGAGGTGGCGCAGATCCAGTCCGCCATCGCCGAACAACCCGCCGGGGCC
GCGGCTTCGATGACAGCGTG

Downstream 100 bases:

>100_bases
TGTCGTATTGCTCCTGGTGGAACGTCGATTCCACCTGGAGAGAGATCGGCTTGCCGATGAAGTCCCCGAGCATCGCCAGA
TGCTGGCTTTCTTCCTCGAG

Product: Drug resistance transporter Bcr/CflA subfamily

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MSEVDVPGAVVAAGAEAGPRRPSVGSMAALTAGLSMLGQFAIATYLPAFAAIANALHASTAEVQQSLTAYLLPFALLLPW
HGAISDAIGRRRMILAGCVFFVAGSLVCAGATGIDMFYVGRALQGVGAGIGVIVGRAMVRDTFDGMQAQKVMALIAMIFA
LAPALAPICGGWLLVWMGWRSIFVFLALISGSLLVSCWIWVPETLPPACRHPMHPKVLARGYFAMLSSRRFMSLALANAG
VNLAIYIYVFAAPRFVYQHLGLGAQSFGWLFIPIVAGIIAGALVAHRIAGRTTPESSVNRGHVVMMAAGVFNLAVAALHL
PPMPWALVALPVFAAGMMMTQPSLQLLALDCFPERRGLASSGYVTAQQIGNLLSSALLVPLLLDSTMKMALGMAGLQIAG
LVAFHVALRGRRVGVTSE

Sequences:

>Translated_418_residues
MSEVDVPGAVVAAGAEAGPRRPSVGSMAALTAGLSMLGQFAIATYLPAFAAIANALHASTAEVQQSLTAYLLPFALLLPW
HGAISDAIGRRRMILAGCVFFVAGSLVCAGATGIDMFYVGRALQGVGAGIGVIVGRAMVRDTFDGMQAQKVMALIAMIFA
LAPALAPICGGWLLVWMGWRSIFVFLALISGSLLVSCWIWVPETLPPACRHPMHPKVLARGYFAMLSSRRFMSLALANAG
VNLAIYIYVFAAPRFVYQHLGLGAQSFGWLFIPIVAGIIAGALVAHRIAGRTTPESSVNRGHVVMMAAGVFNLAVAALHL
PPMPWALVALPVFAAGMMMTQPSLQLLALDCFPERRGLASSGYVTAQQIGNLLSSALLVPLLLDSTMKMALGMAGLQIAG
LVAFHVALRGRRVGVTSE
>Mature_417_residues
SEVDVPGAVVAAGAEAGPRRPSVGSMAALTAGLSMLGQFAIATYLPAFAAIANALHASTAEVQQSLTAYLLPFALLLPWH
GAISDAIGRRRMILAGCVFFVAGSLVCAGATGIDMFYVGRALQGVGAGIGVIVGRAMVRDTFDGMQAQKVMALIAMIFAL
APALAPICGGWLLVWMGWRSIFVFLALISGSLLVSCWIWVPETLPPACRHPMHPKVLARGYFAMLSSRRFMSLALANAGV
NLAIYIYVFAAPRFVYQHLGLGAQSFGWLFIPIVAGIIAGALVAHRIAGRTTPESSVNRGHVVMMAAGVFNLAVAALHLP
PMPWALVALPVFAAGMMMTQPSLQLLALDCFPERRGLASSGYVTAQQIGNLLSSALLVPLLLDSTMKMALGMAGLQIAGL
VAFHVALRGRRVGVTSE

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=346, Percent_Identity=28.6127167630058, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787065, Length=332, Percent_Identity=28.6144578313253, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1790146, Length=333, Percent_Identity=27.3273273273273, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI48994889, Length=251, Percent_Identity=29.8804780876494, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI87082312, Length=169, Percent_Identity=33.7278106508876, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790794, Length=218, Percent_Identity=27.5229357798165, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790195, Length=137, Percent_Identity=31.3868613138686, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43883; Mature: 43752

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVDVPGAVVAAGAEAGPRRPSVGSMAALTAGLSMLGQFAIATYLPAFAAIANALHAST
CCCCCCCCHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEVQQSLTAYLLPFALLLPWHGAISDAIGRRRMILAGCVFFVAGSLVCAGATGIDMFYVG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RALQGVGAGIGVIVGRAMVRDTFDGMQAQKVMALIAMIFALAPALAPICGGWLLVWMGWR
HHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIFVFLALISGSLLVSCWIWVPETLPPACRHPMHPKVLARGYFAMLSSRRFMSLALANAG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCC
VNLAIYIYVFAAPRFVYQHLGLGAQSFGWLFIPIVAGIIAGALVAHRIAGRTTPESSVNR
CCEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GHVVMMAAGVFNLAVAALHLPPMPWALVALPVFAAGMMMTQPSLQLLALDCFPERRGLAS
CEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHEEEEECCHHHCCCCC
SGYVTAQQIGNLLSSALLVPLLLDSTMKMALGMAGLQIAGLVAFHVALRGRRVGVTSE
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SEVDVPGAVVAAGAEAGPRRPSVGSMAALTAGLSMLGQFAIATYLPAFAAIANALHAST
CCCCCCCHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEVQQSLTAYLLPFALLLPWHGAISDAIGRRRMILAGCVFFVAGSLVCAGATGIDMFYVG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RALQGVGAGIGVIVGRAMVRDTFDGMQAQKVMALIAMIFALAPALAPICGGWLLVWMGWR
HHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIFVFLALISGSLLVSCWIWVPETLPPACRHPMHPKVLARGYFAMLSSRRFMSLALANAG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCC
VNLAIYIYVFAAPRFVYQHLGLGAQSFGWLFIPIVAGIIAGALVAHRIAGRTTPESSVNR
CCEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GHVVMMAAGVFNLAVAALHLPPMPWALVALPVFAAGMMMTQPSLQLLALDCFPERRGLAS
CEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHEEEEECCHHHCCCCC
SGYVTAQQIGNLLSSALLVPLLLDSTMKMALGMAGLQIAGLVAFHVALRGRRVGVTSE
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]