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The map label for this gene is dadA [H]

Identifier: 94309685

GI number: 94309685

Start: 818538

End: 819842

Strand: Reverse

Name: dadA [H]

Synonym: Rmet_0740

Alternate gene names: 94309685

Gene position: 819842-818538 (Counterclockwise)

Preceding gene: 94309687

Following gene: 94309677

Centisome position: 20.87

GC content: 66.28

Gene sequence:

>1305_bases
ATGCGCGTTCTAATTCTTGGCAGTGGCGTTATTGGCGTCACCAGTGCGTGGTATCTGGCCCGCGCCGGTCACGAAGTGAT
CGTGGTAGACCGCGAAGGCGGCCCCGCTCTTGGCACCAGCTTTGCCAACGCCGGCCAGATCTCTCCGGGCTATGCGTCGC
CGTGGGCAGCACCCGGCGTTCCGCTCAAGGCGATCAAGTGGATGTTCCAGGAGCACGCGCCGCTCACGATCAAACCCGAC
GGCACGCTGTTCCAGCTTCAGTGGATGTGGCAGATGCTGCAGAACTGCACAGCCGACCGCTACGCGGTGAACAAGGAGCG
CATGGTGCGTCTGGCCGAGTACAGCCGGGACTGTATCCGCGCGCTGCGCGCGGATACCGGCATCAGCTATGAAGGCCGCC
AGCAAGGCACGCTCCAGGTGTTCCGCACCGACGCGCAATTGGAAGCCGCGTCGCGCGACATCGCCGTACTCGAGGAAGCC
GGCGTGCCATACGAATTGCTTTCGCGCGAAGAACTCGCCGCGAGCGAACCGGCGCTCGCCGCCGTCGGCCACAAGCTGGC
CGGTGGCCTGCGCCTGCCGAACGATGAAACCGGGGACTGCCAGCTGTTTACGGAACGGCTGGCAGCCATGGCTGAATCGA
TCGGCGTGCAGTTCATCTACAACCGCTCGATCGACGGCCTGCTGACGCAGGGCGACGCCGTGACCGGCGCAATCGTCGAA
GGGCAACCGATGATGGCCGATGCCGTCGTAGTGGCGCTGGGCAGCTGGTCCACGCCGTTCGTGAAGCACGTGCTGCCGGG
CCTGTCGAACCTGCCCGTCTATCCGCTCAAGGGCTTCTCGATCACGGTGCCGCTCACCGATGCATCGAAGAGCCCGGTGT
CGACGGTGCTCGACGAGACATACAAGGTGGCGATCACGCGCTTCGACGACCGCATCCGCGTGGGCGGCATGGCGCAGATC
GTCGGCTATGACCGCTCGCTCGATCCGAATAAGCGCCGCACGCTGGAACACGTGGTGAACGATCTGTTCCCGGGCGCGGG
CGATGTGAGCCGCGCCACGTTCTGGACCGGCCTGCGCCCGATGACACCCGATAGCACTCCGGTGGTCGGCCCCGCTGGCA
TCCGCGGCCTCTGGCTCAACACGGGTCACGGCACGCTTGGGTGGACGATGGCCTGCGGCTCGGGCCAGTTGCTGTCCGAC
CTGGTCTCGGGCACGCGCCCGGCAATCCGTGCCGACGACCTCTCGGTGGATCGCTACCGCAAGCAGCCGCGCACGCACCA
CTTGCCACGGCCGCTACCGGCCTGA

Upstream 100 bases:

>100_bases
CCTGTTTTGCAGAGAAATTCGGTAACTATGACTGCCTTGAAGTCAATAGTATAGAAGCGAATTCTCCCACACATCCATTC
CGCAGTAGAGAGCCCCCAAC

Downstream 100 bases:

>100_bases
TCCTTCGGCACCGTGACGAAAAAAAGGGCGACCCCGAGGGGTCGCCCTTTTCATTGCCTTGATGCCCGGTACGCCGGATC
AGAACTGATCCGCCGTCAGC

Product: D-amino acid dehydrogenase small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MRVLILGSGVIGVTSAWYLARAGHEVIVVDREGGPALGTSFANAGQISPGYASPWAAPGVPLKAIKWMFQEHAPLTIKPD
GTLFQLQWMWQMLQNCTADRYAVNKERMVRLAEYSRDCIRALRADTGISYEGRQQGTLQVFRTDAQLEAASRDIAVLEEA
GVPYELLSREELAASEPALAAVGHKLAGGLRLPNDETGDCQLFTERLAAMAESIGVQFIYNRSIDGLLTQGDAVTGAIVE
GQPMMADAVVVALGSWSTPFVKHVLPGLSNLPVYPLKGFSITVPLTDASKSPVSTVLDETYKVAITRFDDRIRVGGMAQI
VGYDRSLDPNKRRTLEHVVNDLFPGAGDVSRATFWTGLRPMTPDSTPVVGPAGIRGLWLNTGHGTLGWTMACGSGQLLSD
LVSGTRPAIRADDLSVDRYRKQPRTHHLPRPLPA

Sequences:

>Translated_434_residues
MRVLILGSGVIGVTSAWYLARAGHEVIVVDREGGPALGTSFANAGQISPGYASPWAAPGVPLKAIKWMFQEHAPLTIKPD
GTLFQLQWMWQMLQNCTADRYAVNKERMVRLAEYSRDCIRALRADTGISYEGRQQGTLQVFRTDAQLEAASRDIAVLEEA
GVPYELLSREELAASEPALAAVGHKLAGGLRLPNDETGDCQLFTERLAAMAESIGVQFIYNRSIDGLLTQGDAVTGAIVE
GQPMMADAVVVALGSWSTPFVKHVLPGLSNLPVYPLKGFSITVPLTDASKSPVSTVLDETYKVAITRFDDRIRVGGMAQI
VGYDRSLDPNKRRTLEHVVNDLFPGAGDVSRATFWTGLRPMTPDSTPVVGPAGIRGLWLNTGHGTLGWTMACGSGQLLSD
LVSGTRPAIRADDLSVDRYRKQPRTHHLPRPLPA
>Mature_434_residues
MRVLILGSGVIGVTSAWYLARAGHEVIVVDREGGPALGTSFANAGQISPGYASPWAAPGVPLKAIKWMFQEHAPLTIKPD
GTLFQLQWMWQMLQNCTADRYAVNKERMVRLAEYSRDCIRALRADTGISYEGRQQGTLQVFRTDAQLEAASRDIAVLEEA
GVPYELLSREELAASEPALAAVGHKLAGGLRLPNDETGDCQLFTERLAAMAESIGVQFIYNRSIDGLLTQGDAVTGAIVE
GQPMMADAVVVALGSWSTPFVKHVLPGLSNLPVYPLKGFSITVPLTDASKSPVSTVLDETYKVAITRFDDRIRVGGMAQI
VGYDRSLDPNKRRTLEHVVNDLFPGAGDVSRATFWTGLRPMTPDSTPVVGPAGIRGLWLNTGHGTLGWTMACGSGQLLSD
LVSGTRPAIRADDLSVDRYRKQPRTHHLPRPLPA

Specific function: Oxidative deamination of D-amino acids [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=421, Percent_Identity=66.7458432304038, Blast_Score=580, Evalue=1e-167,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023080
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.99.1 [H]

Molecular weight: Translated: 46969; Mature: 46969

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLILGSGVIGVTSAWYLARAGHEVIVVDREGGPALGTSFANAGQISPGYASPWAAPGV
CEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PLKAIKWMFQEHAPLTIKPDGTLFQLQWMWQMLQNCTADRYAVNKERMVRLAEYSRDCIR
CHHHHHHHHHCCCCEEECCCCCEEHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHH
ALRADTGISYEGRQQGTLQVFRTDAQLEAASRDIAVLEEAGVPYELLSREELAASEPALA
HHHHCCCCCCCCCCCCEEEEEEECCHHHHCCCCEEEHHHCCCCHHHHCHHHHHCCCCHHH
AVGHKLAGGLRLPNDETGDCQLFTERLAAMAESIGVQFIYNRSIDGLLTQGDAVTGAIVE
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCEEECCCCEEEEEEC
GQPMMADAVVVALGSWSTPFVKHVLPGLSNLPVYPLKGFSITVPLTDASKSPVSTVLDET
CCCCHHCEEEEEECCCCCHHHHHHHCCCCCCCCEEECCCEEEEEECCCCCCHHHHHHHHH
YKVAITRFDDRIRVGGMAQIVGYDRSLDPNKRRTLEHVVNDLFPGAGDVSRATFWTGLRP
HEEEEEEECCCEEECCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCC
MTPDSTPVVGPAGIRGLWLNTGHGTLGWTMACGSGQLLSDLVSGTRPAIRADDLSVDRYR
CCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCHHHHH
KQPRTHHLPRPLPA
CCCCCCCCCCCCCC
>Mature Secondary Structure
MRVLILGSGVIGVTSAWYLARAGHEVIVVDREGGPALGTSFANAGQISPGYASPWAAPGV
CEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PLKAIKWMFQEHAPLTIKPDGTLFQLQWMWQMLQNCTADRYAVNKERMVRLAEYSRDCIR
CHHHHHHHHHCCCCEEECCCCCEEHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHH
ALRADTGISYEGRQQGTLQVFRTDAQLEAASRDIAVLEEAGVPYELLSREELAASEPALA
HHHHCCCCCCCCCCCCEEEEEEECCHHHHCCCCEEEHHHCCCCHHHHCHHHHHCCCCHHH
AVGHKLAGGLRLPNDETGDCQLFTERLAAMAESIGVQFIYNRSIDGLLTQGDAVTGAIVE
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCEEECCCCEEEEEEC
GQPMMADAVVVALGSWSTPFVKHVLPGLSNLPVYPLKGFSITVPLTDASKSPVSTVLDET
CCCCHHCEEEEEECCCCCHHHHHHHCCCCCCCCEEECCCEEEEEECCCCCCHHHHHHHHH
YKVAITRFDDRIRVGGMAQIVGYDRSLDPNKRRTLEHVVNDLFPGAGDVSRATFWTGLRP
HEEEEEEECCCEEECCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCC
MTPDSTPVVGPAGIRGLWLNTGHGTLGWTMACGSGQLLSDLVSGTRPAIRADDLSVDRYR
CCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCHHHHH
KQPRTHHLPRPLPA
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]