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The map label for this gene is mltB [H]

Identifier: 94309677

GI number: 94309677

Start: 809732

End: 810958

Strand: Reverse

Name: mltB [H]

Synonym: Rmet_0732

Alternate gene names: 94309677

Gene position: 810958-809732 (Counterclockwise)

Preceding gene: 94309685

Following gene: 94309665

Centisome position: 20.65

GC content: 65.04

Gene sequence:

>1227_bases
ATGACGTGCCCCGCGCTTCTACGCCTGTTGGCTGTCGTGTACGGCGCACCGAAGTACGCCCTCGACGAGGCGTCGCGTAC
ATCGCATCCGCTATACTCGGTGCGACTTGCAAAGGACAACATGACCGACCACCAGCGCACCCTGCGCCGCCCGCTGCTGG
GCGCCGCACTCACCGCTGCCGCATTGGGCCTGTGCGGCATTTCTCCTCGGCTTCTCGCCGCCGGCAAGCGCCGCGTGAGC
GTCCGGGAAGAAGAGATCGAACCGGGCCGCTATCAGAATCATCCGCAAACCCGCGCATTCATCGACGAGATGGTGGCCAA
CAACGGCTTCCAGCGGGCCGAACTCGAGGGATGGTTCAGCCAGGCGGTCTATTCCACGACGGTCGCACGACTGATCATGC
CGCCGACCACCCCGGGCAAGAAAAGCTGGCGGGCGTATCGGTCACGCTTCATCGAACCGATCCGCATCAACGCCGGCGTG
CGCTTCTGGCAGCAGAACCGCGAGACGCTGCGTCGCGCCGAGGCCGAGTTCGGCGTGCCGGCATCGGTCATCGTCGGCAT
CATCGGGGTGGAGACGATCTACGGGCGCGACATGGGCAACTTCCGCGTCCTGGACTCGCTCTCGACGCTGGCGTTCGACT
ACCCCGACACCCCGAACCGTGAGGCGCGCACCAAGCTCTTCCGGAATCAGCTCGCCGACTACCTGCTCTGGTGCCGCGAC
ACCAAGACCGACGTATTTTCGGTGCTGGGCTCCTATGCCGGCGCAATCGGCATTCCGCAGTTCATGCCCACGAGCCTGCG
CGAGTACGCGGTCGACTACGACGGCGACGGGCATATCGACCTGCGCAACAGCCCGACCGATGCCATTGGCAGCGTCGGCC
GATTCCTGCAACTGCATGGCTGGGAACAGGGACGTCCGGTGGTCTGGCAGATCGCCAACGACGATGGCAGCCGAGGCGTT
GCCGCGGCTGCGGCCGACGGCGAACCGTGGCCGACACGCACACTCAACCAGCTCACGCGCGCCGGACTTCGCGTAGACGA
ACCGATCGACGTCACCCGCGAAGGCGAAACAGGCGTGTTGATTGCCGATCTCCCGACGCCCGACCAGCCGACCGAGTACG
TGATCGGCCTGCGCAATTTCTACGTGCTGACGCGGTACAACCGAAGCTTCTTCTATGCGCTGACGGTGTATCAGCTTGGG
GAAGCCGTGAAGGCCGCCATGGCCTGA

Upstream 100 bases:

>100_bases
AAATGCCCCATCTCATGCATCAGGAACGACGGATGCGTGTAGTAGCCCGTTGACATTATTCGTTATGGGTCAAGTTCGTC
TCCAGACGCTACGTTAGCAC

Downstream 100 bases:

>100_bases
ACGCCAAGCGGCAAGAGCAAAGAAAAAGGCCCCGCGGCGCAAGCCTGCGGGGCCTTTCTTTCAGGGCAAGTCCGGGCGAT
CAGGCCGGGAACACGCCCGT

Product: membrane-bound lytic murein transglycosylase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MTCPALLRLLAVVYGAPKYALDEASRTSHPLYSVRLAKDNMTDHQRTLRRPLLGAALTAAALGLCGISPRLLAAGKRRVS
VREEEIEPGRYQNHPQTRAFIDEMVANNGFQRAELEGWFSQAVYSTTVARLIMPPTTPGKKSWRAYRSRFIEPIRINAGV
RFWQQNRETLRRAEAEFGVPASVIVGIIGVETIYGRDMGNFRVLDSLSTLAFDYPDTPNREARTKLFRNQLADYLLWCRD
TKTDVFSVLGSYAGAIGIPQFMPTSLREYAVDYDGDGHIDLRNSPTDAIGSVGRFLQLHGWEQGRPVVWQIANDDGSRGV
AAAAADGEPWPTRTLNQLTRAGLRVDEPIDVTREGETGVLIADLPTPDQPTEYVIGLRNFYVLTRYNRSFFYALTVYQLG
EAVKAAMA

Sequences:

>Translated_408_residues
MTCPALLRLLAVVYGAPKYALDEASRTSHPLYSVRLAKDNMTDHQRTLRRPLLGAALTAAALGLCGISPRLLAAGKRRVS
VREEEIEPGRYQNHPQTRAFIDEMVANNGFQRAELEGWFSQAVYSTTVARLIMPPTTPGKKSWRAYRSRFIEPIRINAGV
RFWQQNRETLRRAEAEFGVPASVIVGIIGVETIYGRDMGNFRVLDSLSTLAFDYPDTPNREARTKLFRNQLADYLLWCRD
TKTDVFSVLGSYAGAIGIPQFMPTSLREYAVDYDGDGHIDLRNSPTDAIGSVGRFLQLHGWEQGRPVVWQIANDDGSRGV
AAAAADGEPWPTRTLNQLTRAGLRVDEPIDVTREGETGVLIADLPTPDQPTEYVIGLRNFYVLTRYNRSFFYALTVYQLG
EAVKAAMA
>Mature_407_residues
TCPALLRLLAVVYGAPKYALDEASRTSHPLYSVRLAKDNMTDHQRTLRRPLLGAALTAAALGLCGISPRLLAAGKRRVSV
REEEIEPGRYQNHPQTRAFIDEMVANNGFQRAELEGWFSQAVYSTTVARLIMPPTTPGKKSWRAYRSRFIEPIRINAGVR
FWQQNRETLRRAEAEFGVPASVIVGIIGVETIYGRDMGNFRVLDSLSTLAFDYPDTPNREARTKLFRNQLADYLLWCRDT
KTDVFSVLGSYAGAIGIPQFMPTSLREYAVDYDGDGHIDLRNSPTDAIGSVGRFLQLHGWEQGRPVVWQIANDDGSRGVA
AAAADGEPWPTRTLNQLTRAGLRVDEPIDVTREGETGVLIADLPTPDQPTEYVIGLRNFYVLTRYNRSFFYALTVYQLGE
AVKAAMA

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=330, Percent_Identity=41.2121212121212, Blast_Score=227, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 45534; Mature: 45403

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCPALLRLLAVVYGAPKYALDEASRTSHPLYSVRLAKDNMTDHQRTLRRPLLGAALTAA
CCCHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
ALGLCGISPRLLAAGKRRVSVREEEIEPGRYQNHPQTRAFIDEMVANNGFQRAELEGWFS
HHHHCCCCHHHHHCCCHHCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
QAVYSTTVARLIMPPTTPGKKSWRAYRSRFIEPIRINAGVRFWQQNRETLRRAEAEFGVP
HHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHCCCC
ASVIVGIIGVETIYGRDMGNFRVLDSLSTLAFDYPDTPNREARTKLFRNQLADYLLWCRD
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHCC
TKTDVFSVLGSYAGAIGIPQFMPTSLREYAVDYDGDGHIDLRNSPTDAIGSVGRFLQLHG
CHHHHHHHHHHHHCCCCCCHHCHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHCC
WEQGRPVVWQIANDDGSRGVAAAAADGEPWPTRTLNQLTRAGLRVDEPIDVTREGETGVL
CCCCCCEEEEEECCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCEECCCCCEE
IADLPTPDQPTEYVIGLRNFYVLTRYNRSFFYALTVYQLGEAVKAAMA
EEECCCCCCCHHHHEEHHHEEEEEEECCEEEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TCPALLRLLAVVYGAPKYALDEASRTSHPLYSVRLAKDNMTDHQRTLRRPLLGAALTAA
CCHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
ALGLCGISPRLLAAGKRRVSVREEEIEPGRYQNHPQTRAFIDEMVANNGFQRAELEGWFS
HHHHCCCCHHHHHCCCHHCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
QAVYSTTVARLIMPPTTPGKKSWRAYRSRFIEPIRINAGVRFWQQNRETLRRAEAEFGVP
HHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHCCCC
ASVIVGIIGVETIYGRDMGNFRVLDSLSTLAFDYPDTPNREARTKLFRNQLADYLLWCRD
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHCC
TKTDVFSVLGSYAGAIGIPQFMPTSLREYAVDYDGDGHIDLRNSPTDAIGSVGRFLQLHG
CHHHHHHHHHHHHCCCCCCHHCHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHCC
WEQGRPVVWQIANDDGSRGVAAAAADGEPWPTRTLNQLTRAGLRVDEPIDVTREGETGVL
CCCCCCEEEEEECCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCEECCCCCEE
IADLPTPDQPTEYVIGLRNFYVLTRYNRSFFYALTVYQLGEAVKAAMA
EEECCCCCCCHHHHEEHHHEEEEEEECCEEEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]