Click here to switch to the map view.

The map label for this gene is pheA [H]

Identifier: 94309661

GI number: 94309661

Start: 793301

End: 794464

Strand: Direct

Name: pheA [H]

Synonym: Rmet_0716

Alternate gene names: 94309661

Gene position: 793301-794464 (Clockwise)

Preceding gene: 94309660

Following gene: 94309662

Centisome position: 20.2

GC content: 64.52

Gene sequence:

>1164_bases
ATGACAGAGCAAGACAAGTCCCCCGCCGCCGATGCCCCCGCACAGGAAGCCGGGCGCCAGGACGCTCCTGGACCCAGCGC
GCAGGAGCAGGCGTTGTCGGCCGATCTGGCGCCGTTGCGCACCCAGATCGACACCGTCGACCGTGAACTGCTGGCGCTGC
TCAATCGTCGCGCCCAGCTCGCCCTCGACGTGGGCGAGGTCAAGAAGAAGTACGGCGCCCCGGTATTTCGTCCCGAACGC
GAGTTGCAGGTGATCCGCAAGGTGCAGGGCGCCAATCCCGGACCGCTGCTCGACGAAAGCGTGGCATCGATTTGGCGCGA
GGTGATGTCCGCGTGCCGCGGCCTGGAAAAGCCGCTCGAGATCGCCTTCCTGGGCCCGGCCGGAACGTTCTCCGAGCAGG
CGCTCTACGCGCACTTCGGCCACGAGGTGACGGGCGTGCCGTGCCCCAGCATTGACGAGGTGTTCCGTGCCGTGGAGGCT
GGTACCGTCGAGTACGGCGTGGTGCCGGTAGAGAACTCGACTGAGGGCGCGGTGTCGCGCACGCTCGATCTGTTCCTGCA
GACGTCGCTCAAGATCAGCGGCGAGATCGCGCTGAAGGTCCACCACAATCTCATGGCTTCCACGCCGGACATGAAGGGTG
TGACCGTCGTGCGTGCCCATGCGCAGGCGCTGGCGCAGTGCCAGAACTGGCTGTCCGCGAACTATCCGCATCTGGAGCGC
CAGGCCGTGTCCAGCAATGCCGAAGCCGCACGCATGGCCAGTGAGGACCCTACCGTTGCGGCGATTGCCGGTGAAACGGC
GGCCAATCGCTATCACCTGCACATTATCCGTTCGCACATCCAGGACGATCCGCACAATCGCACCCGTTTCGCGGTGATCG
GCCGCTATGAGACGGAGCCTTCTGGCAGCGATCAGACCTCGTTGATCCTGTCGGTGCCGAACAAGGCGGGCGCCGTGTAT
CAACTGCTGGCACCACTGGCCGAGAACGGCGTATCGATGTGCAGGTTTGAATCGCGCCCGGCGCGCAGTGGCGCGTGGGA
ATACTACTTCTACGTGGATGTGGAAGGCCATCAGCATGAACCGGCCGTGACGCGCGCGATCGAGATGCTGCGTCGCAATG
CCGCGTACCTGAAGGTCCTGGGTTCGTATCCATCCAGCAAATAA

Upstream 100 bases:

>100_bases
CGGCGGTCAAAGCGTCGCGCCGCGCACAGCCATGCGCGCGGCGTGGCTTCGCCCCAGACTTTCCGAGGCCGCCTTTTCGC
GCGGCCCGCCAGGCCTTGAT

Downstream 100 bases:

>100_bases
CGGCAGCGAGGAGACAGACATGGCAGGGCAGGACAACGCGGCTGACTACTTTGGTCCGTCCTATGTGCGCAAGATCTCGC
CGTATATCGCGGGCAAGCCG

Product: prephenate dehydratase, Chorismate mutase

Products: NA

Alternate protein names: Chorismate mutase; CM; Prephenate dehydratase; PDT [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MTEQDKSPAADAPAQEAGRQDAPGPSAQEQALSADLAPLRTQIDTVDRELLALLNRRAQLALDVGEVKKKYGAPVFRPER
ELQVIRKVQGANPGPLLDESVASIWREVMSACRGLEKPLEIAFLGPAGTFSEQALYAHFGHEVTGVPCPSIDEVFRAVEA
GTVEYGVVPVENSTEGAVSRTLDLFLQTSLKISGEIALKVHHNLMASTPDMKGVTVVRAHAQALAQCQNWLSANYPHLER
QAVSSNAEAARMASEDPTVAAIAGETAANRYHLHIIRSHIQDDPHNRTRFAVIGRYETEPSGSDQTSLILSVPNKAGAVY
QLLAPLAENGVSMCRFESRPARSGAWEYYFYVDVEGHQHEPAVTRAIEMLRRNAAYLKVLGSYPSSK

Sequences:

>Translated_387_residues
MTEQDKSPAADAPAQEAGRQDAPGPSAQEQALSADLAPLRTQIDTVDRELLALLNRRAQLALDVGEVKKKYGAPVFRPER
ELQVIRKVQGANPGPLLDESVASIWREVMSACRGLEKPLEIAFLGPAGTFSEQALYAHFGHEVTGVPCPSIDEVFRAVEA
GTVEYGVVPVENSTEGAVSRTLDLFLQTSLKISGEIALKVHHNLMASTPDMKGVTVVRAHAQALAQCQNWLSANYPHLER
QAVSSNAEAARMASEDPTVAAIAGETAANRYHLHIIRSHIQDDPHNRTRFAVIGRYETEPSGSDQTSLILSVPNKAGAVY
QLLAPLAENGVSMCRFESRPARSGAWEYYFYVDVEGHQHEPAVTRAIEMLRRNAAYLKVLGSYPSSK
>Mature_386_residues
TEQDKSPAADAPAQEAGRQDAPGPSAQEQALSADLAPLRTQIDTVDRELLALLNRRAQLALDVGEVKKKYGAPVFRPERE
LQVIRKVQGANPGPLLDESVASIWREVMSACRGLEKPLEIAFLGPAGTFSEQALYAHFGHEVTGVPCPSIDEVFRAVEAG
TVEYGVVPVENSTEGAVSRTLDLFLQTSLKISGEIALKVHHNLMASTPDMKGVTVVRAHAQALAQCQNWLSANYPHLERQ
AVSSNAEAARMASEDPTVAAIAGETAANRYHLHIIRSHIQDDPHNRTRFAVIGRYETEPSGSDQTSLILSVPNKAGAVYQ
LLAPLAENGVSMCRFESRPARSGAWEYYFYVDVEGHQHEPAVTRAIEMLRRNAAYLKVLGSYPSSK

Specific function: L-phenylalanine biosynthesis. [C]

COG id: COG0077

COG function: function code E; Prephenate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 prephenate dehydratase domain [H]

Homologues:

Organism=Escherichia coli, GI1788951, Length=373, Percent_Identity=31.6353887399464, Blast_Score=172, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6324013, Length=299, Percent_Identity=26.0869565217391, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR008242
- InterPro:   IPR002701
- InterPro:   IPR020822
- InterPro:   IPR010957
- InterPro:   IPR001086
- InterPro:   IPR018528 [H]

Pfam domain/function: PF01842 ACT; PF01817 CM_2; PF00800 PDT [H]

EC number: =5.4.99.5; =4.2.1.51 [H]

Molecular weight: Translated: 42141; Mature: 42010

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQDKSPAADAPAQEAGRQDAPGPSAQEQALSADLAPLRTQIDTVDRELLALLNRRAQL
CCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ALDVGEVKKKYGAPVFRPERELQVIRKVQGANPGPLLDESVASIWREVMSACRGLEKPLE
EECHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCE
IAFLGPAGTFSEQALYAHFGHEVTGVPCPSIDEVFRAVEAGTVEYGVVPVENSTEGAVSR
EEEECCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHH
TLDLFLQTSLKISGEIALKVHHNLMASTPDMKGVTVVRAHAQALAQCQNWLSANYPHLER
HHHHHHHHCEEECCEEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
QAVSSNAEAARMASEDPTVAAIAGETAANRYHLHIIRSHIQDDPHNRTRFAVIGRYETEP
HHHHCCCHHHHHCCCCCCEEEEECCCCCCCEEHHHHHHHHCCCCCCCEEEEEEEEEECCC
SGSDQTSLILSVPNKAGAVYQLLAPLAENGVSMCRFESRPARSGAWEYYFYVDVEGHQHE
CCCCCCEEEEECCCCCHHHHHHHHHHHHCCHHEEEECCCCCCCCCEEEEEEEEECCCCCC
PAVTRAIEMLRRNAAYLKVLGSYPSSK
HHHHHHHHHHHCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
TEQDKSPAADAPAQEAGRQDAPGPSAQEQALSADLAPLRTQIDTVDRELLALLNRRAQL
CCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ALDVGEVKKKYGAPVFRPERELQVIRKVQGANPGPLLDESVASIWREVMSACRGLEKPLE
EECHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCE
IAFLGPAGTFSEQALYAHFGHEVTGVPCPSIDEVFRAVEAGTVEYGVVPVENSTEGAVSR
EEEECCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHH
TLDLFLQTSLKISGEIALKVHHNLMASTPDMKGVTVVRAHAQALAQCQNWLSANYPHLER
HHHHHHHHCEEECCEEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
QAVSSNAEAARMASEDPTVAAIAGETAANRYHLHIIRSHIQDDPHNRTRFAVIGRYETEP
HHHHCCCHHHHHCCCCCCEEEEECCCCCCCEEHHHHHHHHCCCCCCCEEEEEEEEEECCC
SGSDQTSLILSVPNKAGAVYQLLAPLAENGVSMCRFESRPARSGAWEYYFYVDVEGHQHE
CCCCCCEEEEECCCCCHHHHHHHHHHHHCCHHEEEECCCCCCCCCEEEEEEEEECCCCCC
PAVTRAIEMLRRNAAYLKVLGSYPSSK
HHHHHHHHHHHCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10094619 [H]