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The map label for this gene is gcvA [H]

Identifier: 94309605

GI number: 94309605

Start: 727435

End: 728376

Strand: Reverse

Name: gcvA [H]

Synonym: Rmet_0660

Alternate gene names: 94309605

Gene position: 728376-727435 (Counterclockwise)

Preceding gene: 94309608

Following gene: 94309604

Centisome position: 18.54

GC content: 67.73

Gene sequence:

>942_bases
ATGACCGATTCGCGCCCCCCGCGTCACTCGCGCTCCCCCCATCTGCCCCCGCTTCAGGCGCTTCGCGCACTGGAGGCAGC
CACGCGCCACCGCAGCTTCACACGCGCAGCCGAAGAGCTGGCCCTGACGCATAGCGCCATCAGCCACCACATCCGCGCGC
TCGAAAACAACCTTGGCACCAAGCTGTTCCAGCGCACCGGCCTCAGCATGACGCCGACCAGCGCCGGCACGCGCCTGGCC
GAACAGGTGCGCGGCGCGCTTGACCAGCTTGAATCGGCCCTGCGGGAAGCCGGCAGTGTGTCAGGCCCACCCGTGGTGCA
ACTGCAGGTCAGTGTGATGGCAGACCTTGCCAACGCCTGGCTGATCCGCCGGCTGCCGAGCCTGCACGCACAGGCGCCCG
AGCTCGATCTGCATCTGCGGCTGCATGCGGAGATTTCGCCGCCCGACCCCTACGCGGTGGACGTCGGCATCTGGCACCAG
CGCGTGGACCTGCCCGGCTTCGAATGCCACAACCTCATCGAGGACTACGTGATCGCCGTGGCCAGCCCTGCGCTGCTGGC
GCGATATCCGGGCTTCACCGTGGCGGACCTGCCCAGGCTGCCGATGCTGCGTTTCGCGCTCCGGCCGTGGCGCGACTGGC
TGCTGCTGGCCGGACTGCCGCCCGACGAACCCGTGCGGGGGCCGATCTTCCAGGATGCCGGCCTGATGCTGCAATCGGCC
GTGGCCGGCCTGGGCGTGGCCACCGCTCGCGCGCAGCTTGCGCACGACTACATCGCCAGCGGACAACTCGTGCAGATCGG
CACCACGCGGATTCCATCGAGCCTGCATTACTGGGTGACCTGGCGCGAAGGCAATCCGCGCGAAAAGGCGATCCTGCGTT
TTCATACCTGGCTCAAGGACCAGATTGCCGGCATTGCGCCGGAAAATGACGCTCAATTGTGA

Upstream 100 bases:

>100_bases
GGATGGGTGGTGGATGCAGTCATGCATGCATTGTCGTCACCGGCATGGTGCAAGACCAGCGAAAGATACCTGCGGCAATT
GTGAGTAGAATTTGACGACC

Downstream 100 bases:

>100_bases
AACAGGTTGCGCATTTGGTTGCATAGCCAGTTTCAGAATTGCAACCATTTGACTCCGAAACTGGCAAATGCGTCCCTTAC
CCCTCCGAACTCATCCGTAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MTDSRPPRHSRSPHLPPLQALRALEAATRHRSFTRAAEELALTHSAISHHIRALENNLGTKLFQRTGLSMTPTSAGTRLA
EQVRGALDQLESALREAGSVSGPPVVQLQVSVMADLANAWLIRRLPSLHAQAPELDLHLRLHAEISPPDPYAVDVGIWHQ
RVDLPGFECHNLIEDYVIAVASPALLARYPGFTVADLPRLPMLRFALRPWRDWLLLAGLPPDEPVRGPIFQDAGLMLQSA
VAGLGVATARAQLAHDYIASGQLVQIGTTRIPSSLHYWVTWREGNPREKAILRFHTWLKDQIAGIAPENDAQL

Sequences:

>Translated_313_residues
MTDSRPPRHSRSPHLPPLQALRALEAATRHRSFTRAAEELALTHSAISHHIRALENNLGTKLFQRTGLSMTPTSAGTRLA
EQVRGALDQLESALREAGSVSGPPVVQLQVSVMADLANAWLIRRLPSLHAQAPELDLHLRLHAEISPPDPYAVDVGIWHQ
RVDLPGFECHNLIEDYVIAVASPALLARYPGFTVADLPRLPMLRFALRPWRDWLLLAGLPPDEPVRGPIFQDAGLMLQSA
VAGLGVATARAQLAHDYIASGQLVQIGTTRIPSSLHYWVTWREGNPREKAILRFHTWLKDQIAGIAPENDAQL
>Mature_312_residues
TDSRPPRHSRSPHLPPLQALRALEAATRHRSFTRAAEELALTHSAISHHIRALENNLGTKLFQRTGLSMTPTSAGTRLAE
QVRGALDQLESALREAGSVSGPPVVQLQVSVMADLANAWLIRRLPSLHAQAPELDLHLRLHAEISPPDPYAVDVGIWHQR
VDLPGFECHNLIEDYVIAVASPALLARYPGFTVADLPRLPMLRFALRPWRDWLLLAGLPPDEPVRGPIFQDAGLMLQSAV
AGLGVATARAQLAHDYIASGQLVQIGTTRIPSSLHYWVTWREGNPREKAILRFHTWLKDQIAGIAPENDAQL

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=291, Percent_Identity=35.0515463917526, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1786448, Length=294, Percent_Identity=30.6122448979592, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1788706, Length=299, Percent_Identity=27.0903010033445, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787128, Length=281, Percent_Identity=27.0462633451957, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI87081978, Length=258, Percent_Identity=27.1317829457364, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1786401, Length=271, Percent_Identity=25.0922509225092, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34533; Mature: 34402

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSRPPRHSRSPHLPPLQALRALEAATRHRSFTRAAEELALTHSAISHHIRALENNLGT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLFQRTGLSMTPTSAGTRLAEQVRGALDQLESALREAGSVSGPPVVQLQVSVMADLANAW
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHH
LIRRLPSLHAQAPELDLHLRLHAEISPPDPYAVDVGIWHQRVDLPGFECHNLIEDYVIAV
HHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEECCHHHHCCCCCHHHHHHHHHHHHHH
ASPALLARYPGFTVADLPRLPMLRFALRPWRDWLLLAGLPPDEPVRGPIFQDAGLMLQSA
CCHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHH
VAGLGVATARAQLAHDYIASGQLVQIGTTRIPSSLHYWVTWREGNPREKAILRFHTWLKD
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHH
QIAGIAPENDAQL
HHCCCCCCCCCCC
>Mature Secondary Structure 
TDSRPPRHSRSPHLPPLQALRALEAATRHRSFTRAAEELALTHSAISHHIRALENNLGT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLFQRTGLSMTPTSAGTRLAEQVRGALDQLESALREAGSVSGPPVVQLQVSVMADLANAW
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHH
LIRRLPSLHAQAPELDLHLRLHAEISPPDPYAVDVGIWHQRVDLPGFECHNLIEDYVIAV
HHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEECCHHHHCCCCCHHHHHHHHHHHHHH
ASPALLARYPGFTVADLPRLPMLRFALRPWRDWLLLAGLPPDEPVRGPIFQDAGLMLQSA
CCHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHH
VAGLGVATARAQLAHDYIASGQLVQIGTTRIPSSLHYWVTWREGNPREKAILRFHTWLKD
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHH
QIAGIAPENDAQL
HHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]