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The map label for this gene is zraR [H]

Identifier: 94309604

GI number: 94309604

Start: 725028

End: 726404

Strand: Reverse

Name: zraR [H]

Synonym: Rmet_0659

Alternate gene names: 94309604

Gene position: 726404-725028 (Counterclockwise)

Preceding gene: 94309605

Following gene: 94309603

Centisome position: 18.49

GC content: 65.72

Gene sequence:

>1377_bases
ATGAGTCACTATGTCAACCGCCCGTTGCTGTATGTCTCCAGGCATCACGACGCGTCGTTGTGCCGTTATCTGTCGCAGCA
CGGCTGGAAGCTGGTGTTCGCGAACAGCCTCGAGGACCTGGCCGCGCTGCCGCTCGATGGACCATTCTCGGCGGGCCTGC
TCGATTTCCAGAGCGGCTTCAGCGATACCGATCTCGAGTTGATGGAGCCATGGCTGGCGACGCGCCAGATCGGCTGGATC
GGTGCGATCAGCTGCGCCGACGAGCTGACAGACACCGCGCGCAAGCTGCTCGGCCTGTACTTCATTGACTACTACACAGC
GCCATTCGACATGGCCGAGCTAAGCCGGACGCTCGGACACGCGGTTGGCATGGCAGCCCTGCGGCCGGGCCGCGAGCAAC
CAGCCGTGCCGCTGACGCGCCCGCACGGGATCATCGGCGAATGCAATGCCATGCAGGCGCTGTTCCGCAGCATCGAGAAG
GTGTCGCGGTGGGACACGCCGGTACTGATCTCCGGTGAATCCGGCACCGGCAAGGAACTCGCCGCGCAGGCCATCCATCG
CGCCTCGCGCCGGGCCAACCAGCCGTTCGTCGCGGTCAACTGTGCGGCCATCCCGCCCACCCTGCTGATGTCCGAACTGT
TCGGCTACGAGCGTGGCGCCTTCACGGGCGCGACCAAGCGCAAGCCGGGCCGGATCGAGATCGCGCAAGGCGGCACGCTG
TTCCTGGATGAAATCGGCGACATGCCGCTCGAGAGCCAGACCAGTCTGCTGCGCTTCCTGGAAACAGGCCGGATCGAACG
GCTGGGTGGTACCGAATCCGTCGCCGTCGATGTCCGGGTCATCTCGGCCACGCACGTCGACCTCGAAGCCGCCGTGGCGA
CCGAGCGGTTCCGTGGCGACCTCTTTCACCGCCTGTGCGTGCTGCGCGTCAAGCAGCCCCCCCTGCGCGAACGCGGCTCC
GATGTGCTGCTGCTGGCCGAACACATCCTGGATTCCGTCCGCAAGGACTCGCCCGAGCATATCCGCGGCTTTTCGCCCAT
GGCCCTGCGTGCCATCACCACTCATGCCTGGCCAGGCAACGTGCGCGAACTGCTCAACCGGATCCGCCTTGCCGCGGCGA
TGTGCGAGAACGGGGTCATCCAGCCCTCCGACCTCGAACTCGACACGCCCGTGGAAAGCAAGCCGCCGACGCTGGCCGCG
ATTCGCGAAGCGGCCGAGCAGGCCGCGATCCTCGAAGCCCTGCAACGCCACCACGAACGTTTGATCGACGTGGCGGCCGA
GCTTGGCATCTCGCGAGTCACGTTGTTCCGACTCATGCGCGACTACAAGCTGCAGGTGCGGAAAGGCAACGTGGGACTGG
TCTGCGTGCAGAACTGA

Upstream 100 bases:

>100_bases
CAGCGTTCCAGCATCCCCCTTCTCCCTCGAGCAACGTGGCCAAAGGCGTCCATGGCGCCTCGCCGCGCAGCGGGAAAAAC
AAAAAAAGCTGGGGGAAAAA

Downstream 100 bases:

>100_bases
AGGCGGGTGTCGATCCACGACGCGGAGTTCGAACATGCTGCAAGTCCAGTCCCGCTCCCGAGACGTGCCGGGCGAGCCAG
CCGGCCATGCCATCTGGCAT

Product: response regulator containing CheY-like receiver

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSHYVNRPLLYVSRHHDASLCRYLSQHGWKLVFANSLEDLAALPLDGPFSAGLLDFQSGFSDTDLELMEPWLATRQIGWI
GAISCADELTDTARKLLGLYFIDYYTAPFDMAELSRTLGHAVGMAALRPGREQPAVPLTRPHGIIGECNAMQALFRSIEK
VSRWDTPVLISGESGTGKELAAQAIHRASRRANQPFVAVNCAAIPPTLLMSELFGYERGAFTGATKRKPGRIEIAQGGTL
FLDEIGDMPLESQTSLLRFLETGRIERLGGTESVAVDVRVISATHVDLEAAVATERFRGDLFHRLCVLRVKQPPLRERGS
DVLLLAEHILDSVRKDSPEHIRGFSPMALRAITTHAWPGNVRELLNRIRLAAAMCENGVIQPSDLELDTPVESKPPTLAA
IREAAEQAAILEALQRHHERLIDVAAELGISRVTLFRLMRDYKLQVRKGNVGLVCVQN

Sequences:

>Translated_458_residues
MSHYVNRPLLYVSRHHDASLCRYLSQHGWKLVFANSLEDLAALPLDGPFSAGLLDFQSGFSDTDLELMEPWLATRQIGWI
GAISCADELTDTARKLLGLYFIDYYTAPFDMAELSRTLGHAVGMAALRPGREQPAVPLTRPHGIIGECNAMQALFRSIEK
VSRWDTPVLISGESGTGKELAAQAIHRASRRANQPFVAVNCAAIPPTLLMSELFGYERGAFTGATKRKPGRIEIAQGGTL
FLDEIGDMPLESQTSLLRFLETGRIERLGGTESVAVDVRVISATHVDLEAAVATERFRGDLFHRLCVLRVKQPPLRERGS
DVLLLAEHILDSVRKDSPEHIRGFSPMALRAITTHAWPGNVRELLNRIRLAAAMCENGVIQPSDLELDTPVESKPPTLAA
IREAAEQAAILEALQRHHERLIDVAAELGISRVTLFRLMRDYKLQVRKGNVGLVCVQN
>Mature_457_residues
SHYVNRPLLYVSRHHDASLCRYLSQHGWKLVFANSLEDLAALPLDGPFSAGLLDFQSGFSDTDLELMEPWLATRQIGWIG
AISCADELTDTARKLLGLYFIDYYTAPFDMAELSRTLGHAVGMAALRPGREQPAVPLTRPHGIIGECNAMQALFRSIEKV
SRWDTPVLISGESGTGKELAAQAIHRASRRANQPFVAVNCAAIPPTLLMSELFGYERGAFTGATKRKPGRIEIAQGGTLF
LDEIGDMPLESQTSLLRFLETGRIERLGGTESVAVDVRVISATHVDLEAAVATERFRGDLFHRLCVLRVKQPPLRERGSD
VLLLAEHILDSVRKDSPEHIRGFSPMALRAITTHAWPGNVRELLNRIRLAAAMCENGVIQPSDLELDTPVESKPPTLAAI
REAAEQAAILEALQRHHERLIDVAAELGISRVTLFRLMRDYKLQVRKGNVGLVCVQN

Specific function: Member of the two-component regulatory system zraS/zraR. When activated by zraS it acts in conjunction with sigma-54 to regulate the expression of zraP. Positively autoregulates the expression of the zraSR operon [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=288, Percent_Identity=49.3055555555556, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI1788550, Length=367, Percent_Identity=40.0544959128065, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1790437, Length=363, Percent_Identity=40.495867768595, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI87082117, Length=325, Percent_Identity=42.1538461538462, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI1789233, Length=324, Percent_Identity=41.9753086419753, Blast_Score=228, Evalue=9e-61,
Organism=Escherichia coli, GI1789087, Length=302, Percent_Identity=42.7152317880795, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1788905, Length=344, Percent_Identity=38.953488372093, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1786524, Length=319, Percent_Identity=44.8275862068966, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI87081872, Length=255, Percent_Identity=43.1372549019608, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI87082152, Length=339, Percent_Identity=41.5929203539823, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI1787583, Length=316, Percent_Identity=34.4936708860759, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI87081858, Length=292, Percent_Identity=34.5890410958904, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1789828, Length=293, Percent_Identity=31.3993174061433, Blast_Score=118, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50604; Mature: 50473

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHYVNRPLLYVSRHHDASLCRYLSQHGWKLVFANSLEDLAALPLDGPFSAGLLDFQSGF
CCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEECCHHHHHCCCCCCCCCCCHHHHHCCC
SDTDLELMEPWLATRQIGWIGAISCADELTDTARKLLGLYFIDYYTAPFDMAELSRTLGH
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AVGMAALRPGREQPAVPLTRPHGIIGECNAMQALFRSIEKVSRWDTPVLISGESGTGKEL
HHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHH
AAQAIHRASRRANQPFVAVNCAAIPPTLLMSELFGYERGAFTGATKRKPGRIEIAQGGTL
HHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCEE
FLDEIGDMPLESQTSLLRFLETGRIERLGGTESVAVDVRVISATHVDLEAAVATERFRGD
EEHHHCCCCCCHHHHHHHHHHCCCEEECCCCCEEEEEEEEEEEECCCHHHHHHHHHHHHH
LFHRLCVLRVKQPPLRERGSDVLLLAEHILDSVRKDSPEHIRGFSPMALRAITTHAWPGN
HHHHHHHHHCCCCCHHHCCCCEEHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHCCCCCCH
VRELLNRIRLAAAMCENGVIQPSDLELDTPVESKPPTLAAIREAAEQAAILEALQRHHER
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LIDVAAELGISRVTLFRLMRDYKLQVRKGNVGLVCVQN
HHHHHHHHCHHHHHHHHHHHHHHEEEEECCEEEEEECC
>Mature Secondary Structure 
SHYVNRPLLYVSRHHDASLCRYLSQHGWKLVFANSLEDLAALPLDGPFSAGLLDFQSGF
CCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEECCHHHHHCCCCCCCCCCCHHHHHCCC
SDTDLELMEPWLATRQIGWIGAISCADELTDTARKLLGLYFIDYYTAPFDMAELSRTLGH
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AVGMAALRPGREQPAVPLTRPHGIIGECNAMQALFRSIEKVSRWDTPVLISGESGTGKEL
HHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHH
AAQAIHRASRRANQPFVAVNCAAIPPTLLMSELFGYERGAFTGATKRKPGRIEIAQGGTL
HHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCEE
FLDEIGDMPLESQTSLLRFLETGRIERLGGTESVAVDVRVISATHVDLEAAVATERFRGD
EEHHHCCCCCCHHHHHHHHHHCCCEEECCCCCEEEEEEEEEEEECCCHHHHHHHHHHHHH
LFHRLCVLRVKQPPLRERGSDVLLLAEHILDSVRKDSPEHIRGFSPMALRAITTHAWPGN
HHHHHHHHHCCCCCHHHCCCCEEHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHCCCCCCH
VRELLNRIRLAAAMCENGVIQPSDLELDTPVESKPPTLAAIREAAEQAAILEALQRHHER
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LIDVAAELGISRVTLFRLMRDYKLQVRKGNVGLVCVQN
HHHHHHHHCHHHHHHHHHHHHHHEEEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11243806 [H]