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The map label for this gene is mrcA [C]

Identifier: 94309503

GI number: 94309503

Start: 597040

End: 597735

Strand: Reverse

Name: mrcA [C]

Synonym: Rmet_0558

Alternate gene names: 94309503

Gene position: 597735-597040 (Counterclockwise)

Preceding gene: 94309504

Following gene: 94309497

Centisome position: 15.22

GC content: 62.5

Gene sequence:

>696_bases
ATGCAACGTCTCTGGTCCGCCCTCGTCAAATTCGTTCTGCTCGCCATCATCGGGAGCGCCCTGCTGCTAGCCTTCGCCAT
CGTGATGGCGAATCGGCAGTTGCCGTCCCTCGACGCGCTGACGGCATTTCGCGATTCGCCCGGCTACGTGAAGATCGCGG
ATTTCCCGCACAACTTGCCCGAGGCCGTGGTCGCCATCGAAGACGACCGCTTCTACCTGCATGACGGCGTGGACTATGTG
GGCGCAATGCGCGCCGGCATTGCCAATCTCTCCGACGATCTCTCGCAAGGCGCGTCGACGATCACGATGCAGGTGGCGCG
TAACTTCTATCTGTCGCGCCAGAAGACCTACACGCGCAAGCTCTACGAAATCCTGCTGGCGTACCGGATCGAGAACGCAC
TGAGCAAGGATGAAATCCTGGAGATCTACCTGAACAAGATCTACCTAGGACAGGGCGCGTATGGCTTCGGCGAGGCTGCC
GAAACCTATTTCGGCAAACCGGTCGACCGGCTCACGCTGGCCGAATGCGCGATGCTGGCCGGGCTGCCCAAGGCGCCATC
CGCCAACAACCCGGTGGTCAATCCGCGCCGCGCTCGCCAGCGCCAGGCCTACATCCTGCTGCGGATGCTGGAACTGGGCC
GCATCACGCGCGCCCAGTACGATGCCGCACTGCTCGAACCGCTGCGGCTCAAGTAG

Upstream 100 bases:

>100_bases
CGGCGGCGTAGGCCATTGACGCACGCTTCGCACGGACCGGTGACAGGAGAGCATCAGAGATTTCCCAGACGCGATAGCCG
CCAATGGCACAATACGCGCC

Downstream 100 bases:

>100_bases
GCTCGCGCGTCACGCGGCCACCTGCGCCACCGCCACCTTGTCGCGATGACGCGCCACGGCAGCCTGCACGATCGGATCGA
GCCCTTCCCCGCCTGTCGTG

Product: putative bifunctional transglycosylase and transpeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MQRLWSALVKFVLLAIIGSALLLAFAIVMANRQLPSLDALTAFRDSPGYVKIADFPHNLPEAVVAIEDDRFYLHDGVDYV
GAMRAGIANLSDDLSQGASTITMQVARNFYLSRQKTYTRKLYEILLAYRIENALSKDEILEIYLNKIYLGQGAYGFGEAA
ETYFGKPVDRLTLAECAMLAGLPKAPSANNPVVNPRRARQRQAYILLRMLELGRITRAQYDAALLEPLRLK

Sequences:

>Translated_231_residues
MQRLWSALVKFVLLAIIGSALLLAFAIVMANRQLPSLDALTAFRDSPGYVKIADFPHNLPEAVVAIEDDRFYLHDGVDYV
GAMRAGIANLSDDLSQGASTITMQVARNFYLSRQKTYTRKLYEILLAYRIENALSKDEILEIYLNKIYLGQGAYGFGEAA
ETYFGKPVDRLTLAECAMLAGLPKAPSANNPVVNPRRARQRQAYILLRMLELGRITRAQYDAALLEPLRLK
>Mature_231_residues
MQRLWSALVKFVLLAIIGSALLLAFAIVMANRQLPSLDALTAFRDSPGYVKIADFPHNLPEAVVAIEDDRFYLHDGVDYV
GAMRAGIANLSDDLSQGASTITMQVARNFYLSRQKTYTRKLYEILLAYRIENALSKDEILEIYLNKIYLGQGAYGFGEAA
ETYFGKPVDRLTLAECAMLAGLPKAPSANNPVVNPRRARQRQAYILLRMLELGRITRAQYDAALLEPLRLK

Specific function: Cell wall formation [H]

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Secreted. Membrane (Potential). Note=Membrane-associated (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 51 family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=178, Percent_Identity=55.0561797752809, Blast_Score=182, Evalue=1e-47,
Organism=Escherichia coli, GI1786343, Length=159, Percent_Identity=40.8805031446541, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1788867, Length=182, Percent_Identity=37.9120879120879, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789601, Length=191, Percent_Identity=34.0314136125654, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001264 [H]

Pfam domain/function: PF00912 Transgly [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 25831; Mature: 25831

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRLWSALVKFVLLAIIGSALLLAFAIVMANRQLPSLDALTAFRDSPGYVKIADFPHNLP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEECCCCCCCC
EAVVAIEDDRFYLHDGVDYVGAMRAGIANLSDDLSQGASTITMQVARNFYLSRQKTYTRK
HHEEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LYEILLAYRIENALSKDEILEIYLNKIYLGQGAYGFGEAAETYFGKPVDRLTLAECAMLA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHH
GLPKAPSANNPVVNPRRARQRQAYILLRMLELGRITRAQYDAALLEPLRLK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQRLWSALVKFVLLAIIGSALLLAFAIVMANRQLPSLDALTAFRDSPGYVKIADFPHNLP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEECCCCCCCC
EAVVAIEDDRFYLHDGVDYVGAMRAGIANLSDDLSQGASTITMQVARNFYLSRQKTYTRK
HHEEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LYEILLAYRIENALSKDEILEIYLNKIYLGQGAYGFGEAAETYFGKPVDRLTLAECAMLA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHH
GLPKAPSANNPVVNPRRARQRQAYILLRMLELGRITRAQYDAALLEPLRLK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8405966 [H]