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The map label for this gene is kshB [H]

Identifier: 94309488

GI number: 94309488

Start: 578673

End: 579692

Strand: Direct

Name: kshB [H]

Synonym: Rmet_0543

Alternate gene names: 94309488

Gene position: 578673-579692 (Clockwise)

Preceding gene: 94309486

Following gene: 94309489

Centisome position: 14.73

GC content: 65.69

Gene sequence:

>1020_bases
ATGCAGTTCCATCGCCTGACCGTTGCCGATGTGGTTGCGGAGACCGAAGACGCACGTTCGTTCGCGCTTGCGATACCCGA
GCCGCTGCGCGGCGCGTTTCGATATCGTGCGGGGCAGCATTTGACCTTCCGGGTGGACGTCGGCGGGGAAACCCTGATGC
GCAGCTACTCGCTGTCCAGTGCGCCGGAGTCCCTTGGGCTACCGGTGGTGACGGTAAAGCGGGTCGCGCAAGGCAGGGTG
TCCAACTGGTTCCACGCGAACGTGGGCCCCGGCAGCGTGCTGGACGTGAGCGAGCCCACCGGGCGCTTCGTGTGTGACGA
TGGCGCGGCGCCGCTGGTGTTCTGCGCGGCGGGTAGTGGCATCACGCCAGTCCTGTCGATGATTCGCTCCGCACTAGCCA
CCACCGCGCGCCACATCACGCTCTTCTATGCGAATCGTGATGCCGCGAGCGTCATCTTTGGGCAGGCCATCGCCAGGCTG
GTGGCGGATCATCCGGAACGGCTCACTGTCCGGTGGCACTACGATGACGAGCACGGCATCCCCGACGCGCAGACCCTCGG
ACAAATGCTGCGGCGCAATGCGGACTTCCACCTTTATCTTTGCGGTCCCGCGCCGTTCATGGCCATGATTCAGCAGGCCG
CGGGCGAAGCCGGCGTGGCGGGTGGGCGTGTGCACCTGGAGCGTTTCGACGCCGCGCCCGTGGTCGCCAGCGCCGCGGTT
ACCGCCACGGCAGCGATGACCTCATGCGATGCGCGCGTGACGATGAAGGGCGAACAGCATACGGTGCGGGTGGAGGGCGG
CCAGTCGCTGCTGCAGGCCATGCTGGAGGCCGGGCTCGATGTGCCATACGCGTGCGAGGAAGGCTACTGCGGATCGTGCG
CCGCAAAATGCCTCGATGGCGAAGTCGCCCATGCGCACAACGACGTGTTCAGCCCTGACGAACTCGCCGCTGGTTGGATA
CTGGCGTGCCAGGCTCGTCCGCGTCACGATCGTCCATTGATCATCACCTACGACGTATGA

Upstream 100 bases:

>100_bases
CCTTGTTCTTGCGGGGGCTTCCGACTATAAACAGAAGAATAATTCTGTTTCTGATTAATGCAAGGACCCGATACAGGTTC
CGCAGAACGAGGAGACGCGG

Downstream 100 bases:

>100_bases
ATCCGACCATTCCGATGCAGACCGTGGAAGGTTTGCGCGCGCCGCGCCAGGAGCGCAGCGAGGCCTCCCTGCGAGCCATG
CTTGCGGCGGGGCGAGGGTT

Product: putative Ferredoxin:Oxidoreductase FAD/NAD(P)-binding:Oxidoreductase FAD-binding region

Products: NA

Alternate protein names: Rieske-type oxygenase; RO [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MQFHRLTVADVVAETEDARSFALAIPEPLRGAFRYRAGQHLTFRVDVGGETLMRSYSLSSAPESLGLPVVTVKRVAQGRV
SNWFHANVGPGSVLDVSEPTGRFVCDDGAAPLVFCAAGSGITPVLSMIRSALATTARHITLFYANRDAASVIFGQAIARL
VADHPERLTVRWHYDDEHGIPDAQTLGQMLRRNADFHLYLCGPAPFMAMIQQAAGEAGVAGGRVHLERFDAAPVVASAAV
TATAAMTSCDARVTMKGEQHTVRVEGGQSLLQAMLEAGLDVPYACEEGYCGSCAAKCLDGEVAHAHNDVFSPDELAAGWI
LACQARPRHDRPLIITYDV

Sequences:

>Translated_339_residues
MQFHRLTVADVVAETEDARSFALAIPEPLRGAFRYRAGQHLTFRVDVGGETLMRSYSLSSAPESLGLPVVTVKRVAQGRV
SNWFHANVGPGSVLDVSEPTGRFVCDDGAAPLVFCAAGSGITPVLSMIRSALATTARHITLFYANRDAASVIFGQAIARL
VADHPERLTVRWHYDDEHGIPDAQTLGQMLRRNADFHLYLCGPAPFMAMIQQAAGEAGVAGGRVHLERFDAAPVVASAAV
TATAAMTSCDARVTMKGEQHTVRVEGGQSLLQAMLEAGLDVPYACEEGYCGSCAAKCLDGEVAHAHNDVFSPDELAAGWI
LACQARPRHDRPLIITYDV
>Mature_339_residues
MQFHRLTVADVVAETEDARSFALAIPEPLRGAFRYRAGQHLTFRVDVGGETLMRSYSLSSAPESLGLPVVTVKRVAQGRV
SNWFHANVGPGSVLDVSEPTGRFVCDDGAAPLVFCAAGSGITPVLSMIRSALATTARHITLFYANRDAASVIFGQAIARL
VADHPERLTVRWHYDDEHGIPDAQTLGQMLRRNADFHLYLCGPAPFMAMIQQAAGEAGVAGGRVHLERFDAAPVVASAAV
TATAAMTSCDARVTMKGEQHTVRVEGGQSLLQAMLEAGLDVPYACEEGYCGSCAAKCLDGEVAHAHNDVFSPDELAAGWI
LACQARPRHDRPLIITYDV

Specific function: Catalyzes the opening of ring B of 1,4-androstadiene- 3,17,-dione (ADD) and 4-androstene-3,17-dione (ADD) with concomitant aromatization of ring A. The ring B is subsequently hydroxylated to yield a catechol and then subject to meta-cleavage [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1787658, Length=356, Percent_Identity=36.2359550561798, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI1787098, Length=335, Percent_Identity=27.7611940298507, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1788104, Length=342, Percent_Identity=25.4385964912281, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788903, Length=228, Percent_Identity=29.8245614035088, Blast_Score=79, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323552, Length=198, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 36329; Mature: 36329

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFHRLTVADVVAETEDARSFALAIPEPLRGAFRYRAGQHLTFRVDVGGETLMRSYSLSS
CCCEEEEHHHHHHCCCCCCEEEEECCHHHHHHHHHCCCCEEEEEEECCHHHHHHHHCCCC
APESLGLPVVTVKRVAQGRVSNWFHANVGPGSVLDVSEPTGRFVCDDGAAPLVFCAAGSG
CCHHHCCCHHHHHHHHCCCCCCEEECCCCCCCEEECCCCCCCEEECCCCCCEEEEECCCC
ITPVLSMIRSALATTARHITLFYANRDAASVIFGQAIARLVADHPERLTVRWHYDDEHGI
HHHHHHHHHHHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCC
PDAQTLGQMLRRNADFHLYLCGPAPFMAMIQQAAGEAGVAGGRVHLERFDAAPVVASAAV
CCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHH
TATAAMTSCDARVTMKGEQHTVRVEGGQSLLQAMLEAGLDVPYACEEGYCGSCAAKCLDG
HHHHHHHCCCCEEEECCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCC
EVAHAHNDVFSPDELAAGWILACQARPRHDRPLIITYDV
CHHHHCCCCCCCHHHCCCEEEEECCCCCCCCCEEEEECC
>Mature Secondary Structure
MQFHRLTVADVVAETEDARSFALAIPEPLRGAFRYRAGQHLTFRVDVGGETLMRSYSLSS
CCCEEEEHHHHHHCCCCCCEEEEECCHHHHHHHHHCCCCEEEEEEECCHHHHHHHHCCCC
APESLGLPVVTVKRVAQGRVSNWFHANVGPGSVLDVSEPTGRFVCDDGAAPLVFCAAGSG
CCHHHCCCHHHHHHHHCCCCCCEEECCCCCCCEEECCCCCCCEEECCCCCCEEEEECCCC
ITPVLSMIRSALATTARHITLFYANRDAASVIFGQAIARLVADHPERLTVRWHYDDEHGI
HHHHHHHHHHHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCC
PDAQTLGQMLRRNADFHLYLCGPAPFMAMIQQAAGEAGVAGGRVHLERFDAAPVVASAAV
CCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHH
TATAAMTSCDARVTMKGEQHTVRVEGGQSLLQAMLEAGLDVPYACEEGYCGSCAAKCLDG
HHHHHHHCCCCEEEECCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCC
EVAHAHNDVFSPDELAAGWILACQARPRHDRPLIITYDV
CHHHHCCCCCCCHHHCCCEEEEECCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA