Click here to switch to the map view.

The map label for this gene is amiC [H]

Identifier: 94309471

GI number: 94309471

Start: 561160

End: 562653

Strand: Direct

Name: amiC [H]

Synonym: Rmet_0526

Alternate gene names: 94309471

Gene position: 561160-562653 (Clockwise)

Preceding gene: 94309470

Following gene: 94309473

Centisome position: 14.29

GC content: 65.26

Gene sequence:

>1494_bases
ATGCTGATCAAGCGACTCGCAACCGACCGACCCGATGGACCCGACGGACTGCTGAAGGCCCGCCGCAAATGGATGGCGCA
GGCGCTGAAAGTCAGTGCCGGTACGGTCGTATTGACCATCGCCGGGCCGCAGATCGCCTTTGGCGCCGGCATTGTCGCGG
TGCGGGTGTGGCCGGCCGAGGACTACACGCGGGTAACCATCGAGTCCGACGAGCGCCTGGTGGCCGTGCACCAGATGATC
CGCAACCCGGACCGTCTCGTGGTCGACATCGACGGGCTCGACCTGTCGCCGACGCTGCGCGAACTCGTGGCGAAGATCAC
GCCGAACGATCCGTATATCCAGTCCGTGCGCGTGGGACAGAACCGCCCGCGCGTGGTGCGGATGGTGTTCGACCTCAAGG
AAGACGTCTCGCCGCAGGTGTTCACGCTTGCGCCGATCAGCGACTACCGCAATCGTCTCGTATTCGACCTGTATCCGGTC
AATCCGCCCGATCCGCTCTGGAAGCTCGTGCGTGATACCGAGGACAAGCAGCGGCGATTCGCCGCCGCGCCGCCGCCTGC
TGGTACCGATGGTGTGTCCGGCGCGCCCGCTGGTGCGGAAGAGGATGCAATCGGCGCGATTGTGCGCAAGTTCGAGGATC
GGGACCAGCTTCCGTCGCCGACTACCGCTCCGCCACCCGCATTGGCCGGCGTCAAGCCCAGGCCCCCCCTGCCGTCTCCC
GCGCCCGTGGCGCCGCCGCCGACGGCGCGGACCAATCTGCCGCCACCCAGCGAATTCAAGATGCGCCGCCTGCTGACCGT
GGCCATCGATCCCGGCCATGGTGGTGAAGACCCGGGGGCGATAGGTGCCGCGGGATCGCGCGAGAAGGATGTCGTGCTGC
AAATCGCCACGCGCCTGCGCGCGAAGATCGACGCGCAGCCCAATATGCGCGCGATGATGACGCGCGATTCGGACTTCTTC
GTGCCGCTCAACGTGCGCGTGCAAAAGGCCCGCCGCGTGCAGGCGGACCTGTTCGTGTCGATTCACGCCGACGCTTTCCT
GTCTCCCGAGGCGCGTGGGGCATCGGTGTTCGCGTTGTCCGAACGCGGCGCGTCAAGCTCGGCCGCGCGCTGGCTGGCCA
ACAAGGAAAACAATGCCGATCTGATCGGCGGTGCCAATATGGGCAACAAGGATGCCCAGGTGGCGCGCGTGCTGCTGGAC
TTGTCCACAACGGCACAGATCAACGACAGCATGCAGGTAGGCAGGTCCGTGCTGCAGGAGATCGGTGGGATCAACAAGCT
GCACAAGGGCAGCGTGGAGCAAGCGGGATTTGCGGTGCTCAAGGCGCCGGATATTCCGTCGATCCTGATCGAGACCGCGT
TCATCAGCAATCCCGAGGAAGAGCGCAAGCTCAACGACGACAGCCATCAGGAGCAACTGGCCAACGCCATCCTGCGAGGC
ATCAAGGCGTACTTCGCGCGCAATCCGCCGCTGTCGAAGAATCCATCGGTGTGA

Upstream 100 bases:

>100_bases
GTGTCTCACATTGGGCCGATAACGGGGCCGATGCGGGCGCGGATCGCCGCATGGCAACGCTGCGCGCCTATACTCCCACT
GGACTTACCCTGCTGAACGC

Downstream 100 bases:

>100_bases
TGGTCGAAGGTGGTCAATGCGGCAGCGGGCCACCCTTCACCATGCAAAAAGAAAACGGCGCCTGGGGCGCCGTTTTCATT
TGATCAACGAGCCTGCCTCA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MLIKRLATDRPDGPDGLLKARRKWMAQALKVSAGTVVLTIAGPQIAFGAGIVAVRVWPAEDYTRVTIESDERLVAVHQMI
RNPDRLVVDIDGLDLSPTLRELVAKITPNDPYIQSVRVGQNRPRVVRMVFDLKEDVSPQVFTLAPISDYRNRLVFDLYPV
NPPDPLWKLVRDTEDKQRRFAAAPPPAGTDGVSGAPAGAEEDAIGAIVRKFEDRDQLPSPTTAPPPALAGVKPRPPLPSP
APVAPPPTARTNLPPPSEFKMRRLLTVAIDPGHGGEDPGAIGAAGSREKDVVLQIATRLRAKIDAQPNMRAMMTRDSDFF
VPLNVRVQKARRVQADLFVSIHADAFLSPEARGASVFALSERGASSSAARWLANKENNADLIGGANMGNKDAQVARVLLD
LSTTAQINDSMQVGRSVLQEIGGINKLHKGSVEQAGFAVLKAPDIPSILIETAFISNPEEERKLNDDSHQEQLANAILRG
IKAYFARNPPLSKNPSV

Sequences:

>Translated_497_residues
MLIKRLATDRPDGPDGLLKARRKWMAQALKVSAGTVVLTIAGPQIAFGAGIVAVRVWPAEDYTRVTIESDERLVAVHQMI
RNPDRLVVDIDGLDLSPTLRELVAKITPNDPYIQSVRVGQNRPRVVRMVFDLKEDVSPQVFTLAPISDYRNRLVFDLYPV
NPPDPLWKLVRDTEDKQRRFAAAPPPAGTDGVSGAPAGAEEDAIGAIVRKFEDRDQLPSPTTAPPPALAGVKPRPPLPSP
APVAPPPTARTNLPPPSEFKMRRLLTVAIDPGHGGEDPGAIGAAGSREKDVVLQIATRLRAKIDAQPNMRAMMTRDSDFF
VPLNVRVQKARRVQADLFVSIHADAFLSPEARGASVFALSERGASSSAARWLANKENNADLIGGANMGNKDAQVARVLLD
LSTTAQINDSMQVGRSVLQEIGGINKLHKGSVEQAGFAVLKAPDIPSILIETAFISNPEEERKLNDDSHQEQLANAILRG
IKAYFARNPPLSKNPSV
>Mature_497_residues
MLIKRLATDRPDGPDGLLKARRKWMAQALKVSAGTVVLTIAGPQIAFGAGIVAVRVWPAEDYTRVTIESDERLVAVHQMI
RNPDRLVVDIDGLDLSPTLRELVAKITPNDPYIQSVRVGQNRPRVVRMVFDLKEDVSPQVFTLAPISDYRNRLVFDLYPV
NPPDPLWKLVRDTEDKQRRFAAAPPPAGTDGVSGAPAGAEEDAIGAIVRKFEDRDQLPSPTTAPPPALAGVKPRPPLPSP
APVAPPPTARTNLPPPSEFKMRRLLTVAIDPGHGGEDPGAIGAAGSREKDVVLQIATRLRAKIDAQPNMRAMMTRDSDFF
VPLNVRVQKARRVQADLFVSIHADAFLSPEARGASVFALSERGASSSAARWLANKENNADLIGGANMGNKDAQVARVLLD
LSTTAQINDSMQVGRSVLQEIGGINKLHKGSVEQAGFAVLKAPDIPSILIETAFISNPEEERKLNDDSHQEQLANAILRG
IKAYFARNPPLSKNPSV

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=460, Percent_Identity=44.5652173913043, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1788776, Length=219, Percent_Identity=49.3150684931507, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1790611, Length=233, Percent_Identity=43.3476394849785, Blast_Score=187, Evalue=9e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 53851; Mature: 53851

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIKRLATDRPDGPDGLLKARRKWMAQALKVSAGTVVLTIAGPQIAFGAGIVAVRVWPAE
CCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCEEEEEEECCC
DYTRVTIESDERLVAVHQMIRNPDRLVVDIDGLDLSPTLRELVAKITPNDPYIQSVRVGQ
CCEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCHHEECCC
NRPRVVRMVFDLKEDVSPQVFTLAPISDYRNRLVFDLYPVNPPDPLWKLVRDTEDKQRRF
CCCCEEHHHHHHHHCCCCCEEEECCHHHHCCCEEEEEECCCCCHHHHHHHHCCCHHHHHC
AAAPPPAGTDGVSGAPAGAEEDAIGAIVRKFEDRDQLPSPTTAPPPALAGVKPRPPLPSP
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
APVAPPPTARTNLPPPSEFKMRRLLTVAIDPGHGGEDPGAIGAAGSREKDVVLQIATRLR
CCCCCCCCCCCCCCCCHHHHHEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
AKIDAQPNMRAMMTRDSDFFVPLNVRVQKARRVQADLFVSIHADAFLSPEARGASVFALS
HHCCCCCCCEEEEECCCCEEEEECEEHHHHHHCCEEEEEEEECCEEECCCCCCCEEEEEE
ERGASSSAARWLANKENNADLIGGANMGNKDAQVARVLLDLSTTAQINDSMQVGRSVLQE
CCCCCHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCHHCCHHHHHHHHHHHH
IGGINKLHKGSVEQAGFAVLKAPDIPSILIETAFISNPEEERKLNDDSHQEQLANAILRG
HCCCCHHCCCCCHHCCEEEEECCCCCHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHH
IKAYFARNPPLSKNPSV
HHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLIKRLATDRPDGPDGLLKARRKWMAQALKVSAGTVVLTIAGPQIAFGAGIVAVRVWPAE
CCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCEEEEEEECCC
DYTRVTIESDERLVAVHQMIRNPDRLVVDIDGLDLSPTLRELVAKITPNDPYIQSVRVGQ
CCEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCHHEECCC
NRPRVVRMVFDLKEDVSPQVFTLAPISDYRNRLVFDLYPVNPPDPLWKLVRDTEDKQRRF
CCCCEEHHHHHHHHCCCCCEEEECCHHHHCCCEEEEEECCCCCHHHHHHHHCCCHHHHHC
AAAPPPAGTDGVSGAPAGAEEDAIGAIVRKFEDRDQLPSPTTAPPPALAGVKPRPPLPSP
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
APVAPPPTARTNLPPPSEFKMRRLLTVAIDPGHGGEDPGAIGAAGSREKDVVLQIATRLR
CCCCCCCCCCCCCCCCHHHHHEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
AKIDAQPNMRAMMTRDSDFFVPLNVRVQKARRVQADLFVSIHADAFLSPEARGASVFALS
HHCCCCCCCEEEEECCCCEEEEECEEHHHHHHCCEEEEEEEECCEEECCCCCCCEEEEEE
ERGASSSAARWLANKENNADLIGGANMGNKDAQVARVLLDLSTTAQINDSMQVGRSVLQE
CCCCCHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCHHCCHHHHHHHHHHHH
IGGINKLHKGSVEQAGFAVLKAPDIPSILIETAFISNPEEERKLNDDSHQEQLANAILRG
HCCCCHHCCCCCHHCCEEEEECCCCCHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHH
IKAYFARNPPLSKNPSV
HHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]