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The map label for this gene is envZ [H]

Identifier: 94309461

GI number: 94309461

Start: 552515

End: 553798

Strand: Direct

Name: envZ [H]

Synonym: Rmet_0516

Alternate gene names: 94309461

Gene position: 552515-553798 (Clockwise)

Preceding gene: 94309460

Following gene: 94309468

Centisome position: 14.07

GC content: 65.42

Gene sequence:

>1284_bases
ATGAAGCTTCGCATCGATACCCTGTTTGGCCGCATCGCGCTGCTGATCGCGGCCGTGTTGGTGATCAGCCACTTTTCGTG
GCTGGCCATCCTACGCATGGACCGGCGCCAGCAGCAGATCGACTATTCGGTCGAGCAGATGATCTTCCAGCTCGACAGTA
TCCAGCGGGCGCTCGATGCGACGCCGCCGTTGCCCCTGCCGAGCCTGGTGGAGGTGGCGGATGCCTCCACCGCCGACGAG
CACGCCTCCGTTCCGCAGGCCGGCCGGGCGCGCCGGCTGGTGGAGCAGTTCACGCGGCGCCTGCCGCCGGGTACCGAGGT
GCGGCTTGAGGACGAACTGTCCACGCCGCGGCTGTGGATCAAGCTTCCCAAGCGTAATCGATGGATCGCCATGCCGATCT
TGTTCGTGCACAACCCGCCGCCGGACAATCGACTGTTGCCGGGCGTGGTGCTGGTGGTAGGGATAGCGATCATCTTCGCA
TTGCTGATCGCGTGGCAGATCCAGCGCCCTGTGCGGGATATGGCCGAGGCGGCGGAGAAGCTGTCGCGCCAGCAACTGGT
GTCGCCGCTGCGCGAGCGCGGACCGCATGAACTGCGGCAACTGATCGAGCGTTTCAATCGCATGGTCGCAGACCTCGTGC
GCATCGATCAGGAACGCAACACAATGCTGGCCGGCATCGCGCACGACCTGAAAACCCCGCTCGCGCGCCTACGATTGCGT
GCGGAGATGCTGTCGGATCCGAAGGCCGCTGCCGGCGTGACGCGCGATGTGGAGTCGATGTCGGCGATCGTCGAGCAGTT
CCTGACCTTCGCGCAAAGCAGCGAGCCTACCGCGCGGCCCGTGCCGGTGGACAAGCGTATCGGCGAACTGGCGGCATCCT
TGCAGGAGCAGGACAGGAAGGTGGTGCTGGAACTGAACGCGGGCGATGGCTTCCGCATGATCGCCACTCAGCTCGACCGG
ATCATCGGCAATCTCGTCGACAACGCCTACGCCTACGGTGAGCCGCCGGTGTGTGTGGCCACGAGCCGCACGCAGGAAGG
CTGGAAGCTGATTGTTGAGGATGAGGGACCGGGGATTCCGGATGACGCGATCGAACGCGTGACGATGCCGTTCGTGCGGC
TCGATCCGGCACGTGGCGGCAATGCGCATTCGGGATTGGGCCTGGCGATCGTCGATCGCCTGGTGCGGCAGGCCGGCGGA
CGCCTGTCCATTGCCAACCGCGAGGAAGGCGGCCTGCGCGTGGAAATGGTGTTTCCGTTCGCGGCCATGGCGCAGGCGGC
TTGA

Upstream 100 bases:

>100_bases
GCCTGCTCGACGAGGACGCCCAGCGCCCGCGCTATATCCAGACCGTACGTGGCCGCGGCTACACCTTTGTGCCGGACGAG
GCCGAGGACAGCATTCCGCA

Downstream 100 bases:

>100_bases
TGCGATAAATCTGCCGATGGTTTTCCCCCTCTTCCACGAATGGGAGAGGGGAGCACAGCTTGTGCTTCAGACGTTCAAAC
CTTCTTCTTCTCCCCGATCC

Product: sensor histidine kinase in two-component regulatory system up with Au

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKLRIDTLFGRIALLIAAVLVISHFSWLAILRMDRRQQQIDYSVEQMIFQLDSIQRALDATPPLPLPSLVEVADASTADE
HASVPQAGRARRLVEQFTRRLPPGTEVRLEDELSTPRLWIKLPKRNRWIAMPILFVHNPPPDNRLLPGVVLVVGIAIIFA
LLIAWQIQRPVRDMAEAAEKLSRQQLVSPLRERGPHELRQLIERFNRMVADLVRIDQERNTMLAGIAHDLKTPLARLRLR
AEMLSDPKAAAGVTRDVESMSAIVEQFLTFAQSSEPTARPVPVDKRIGELAASLQEQDRKVVLELNAGDGFRMIATQLDR
IIGNLVDNAYAYGEPPVCVATSRTQEGWKLIVEDEGPGIPDDAIERVTMPFVRLDPARGGNAHSGLGLAIVDRLVRQAGG
RLSIANREEGGLRVEMVFPFAAMAQAA

Sequences:

>Translated_427_residues
MKLRIDTLFGRIALLIAAVLVISHFSWLAILRMDRRQQQIDYSVEQMIFQLDSIQRALDATPPLPLPSLVEVADASTADE
HASVPQAGRARRLVEQFTRRLPPGTEVRLEDELSTPRLWIKLPKRNRWIAMPILFVHNPPPDNRLLPGVVLVVGIAIIFA
LLIAWQIQRPVRDMAEAAEKLSRQQLVSPLRERGPHELRQLIERFNRMVADLVRIDQERNTMLAGIAHDLKTPLARLRLR
AEMLSDPKAAAGVTRDVESMSAIVEQFLTFAQSSEPTARPVPVDKRIGELAASLQEQDRKVVLELNAGDGFRMIATQLDR
IIGNLVDNAYAYGEPPVCVATSRTQEGWKLIVEDEGPGIPDDAIERVTMPFVRLDPARGGNAHSGLGLAIVDRLVRQAGG
RLSIANREEGGLRVEMVFPFAAMAQAA
>Mature_427_residues
MKLRIDTLFGRIALLIAAVLVISHFSWLAILRMDRRQQQIDYSVEQMIFQLDSIQRALDATPPLPLPSLVEVADASTADE
HASVPQAGRARRLVEQFTRRLPPGTEVRLEDELSTPRLWIKLPKRNRWIAMPILFVHNPPPDNRLLPGVVLVVGIAIIFA
LLIAWQIQRPVRDMAEAAEKLSRQQLVSPLRERGPHELRQLIERFNRMVADLVRIDQERNTMLAGIAHDLKTPLARLRLR
AEMLSDPKAAAGVTRDVESMSAIVEQFLTFAQSSEPTARPVPVDKRIGELAASLQEQDRKVVLELNAGDGFRMIATQLDR
IIGNLVDNAYAYGEPPVCVATSRTQEGWKLIVEDEGPGIPDDAIERVTMPFVRLDPARGGNAHSGLGLAIVDRLVRQAGG
RLSIANREEGGLRVEMVFPFAAMAQAA

Specific function: Member of the two-component regulatory system envZ/ompR involved in the regulation of osmoregulation (genes ompF and ompC). EnvZ functions as a membrane-associated protein kinase that phosphorylates ompR in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1789808, Length=354, Percent_Identity=31.9209039548023, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI1787894, Length=339, Percent_Identity=26.8436578171091, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1790861, Length=289, Percent_Identity=30.4498269896194, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1790346, Length=266, Percent_Identity=25.9398496240602, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787374, Length=286, Percent_Identity=29.020979020979, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790551, Length=284, Percent_Identity=29.2253521126761, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786600, Length=251, Percent_Identity=29.8804780876494, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788393, Length=268, Percent_Identity=25, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87081816, Length=264, Percent_Identity=26.8939393939394, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786783, Length=314, Percent_Identity=25.4777070063694, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790436, Length=214, Percent_Identity=26.6355140186916, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1786912, Length=285, Percent_Identity=27.3684210526316, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47541; Mature: 47541

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRIDTLFGRIALLIAAVLVISHFSWLAILRMDRRQQQIDYSVEQMIFQLDSIQRALDA
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TPPLPLPSLVEVADASTADEHASVPQAGRARRLVEQFTRRLPPGTEVRLEDELSTPRLWI
CCCCCCHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEE
KLPKRNRWIAMPILFVHNPPPDNRLLPGVVLVVGIAIIFALLIAWQIQRPVRDMAEAAEK
EECCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSRQQLVSPLRERGPHELRQLIERFNRMVADLVRIDQERNTMLAGIAHDLKTPLARLRLR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEMLSDPKAAAGVTRDVESMSAIVEQFLTFAQSSEPTARPVPVDKRIGELAASLQEQDRK
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCE
VVLELNAGDGFRMIATQLDRIIGNLVDNAYAYGEPPVCVATSRTQEGWKLIVEDEGPGIP
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEECCCCCCC
DDAIERVTMPFVRLDPARGGNAHSGLGLAIVDRLVRQAGGRLSIANREEGGLRVEMVFPF
HHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCEEEEEECCH
AAMAQAA
HHHHHCC
>Mature Secondary Structure
MKLRIDTLFGRIALLIAAVLVISHFSWLAILRMDRRQQQIDYSVEQMIFQLDSIQRALDA
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TPPLPLPSLVEVADASTADEHASVPQAGRARRLVEQFTRRLPPGTEVRLEDELSTPRLWI
CCCCCCHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEE
KLPKRNRWIAMPILFVHNPPPDNRLLPGVVLVVGIAIIFALLIAWQIQRPVRDMAEAAEK
EECCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSRQQLVSPLRERGPHELRQLIERFNRMVADLVRIDQERNTMLAGIAHDLKTPLARLRLR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEMLSDPKAAAGVTRDVESMSAIVEQFLTFAQSSEPTARPVPVDKRIGELAASLQEQDRK
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCE
VVLELNAGDGFRMIATQLDRIIGNLVDNAYAYGEPPVCVATSRTQEGWKLIVEDEGPGIP
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEECCCCCCC
DDAIERVTMPFVRLDPARGGNAHSGLGLAIVDRLVRQAGGRLSIANREEGGLRVEMVFPF
HHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCEEEEEECCH
AAMAQAA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6292200; 2997120; 9278503; 8132603; 2277041; 2824492; 1323560; 9817206; 10426948 [H]