Click here to switch to the map view.

The map label for this gene is gatA [H]

Identifier: 94309440

GI number: 94309440

Start: 524193

End: 525710

Strand: Direct

Name: gatA [H]

Synonym: Rmet_0495

Alternate gene names: 94309440

Gene position: 524193-525710 (Clockwise)

Preceding gene: 94309439

Following gene: 94309441

Centisome position: 13.34

GC content: 69.04

Gene sequence:

>1518_bases
ATGGGCTCGGTCCAAGATGACAACGGAGCTACCGTGACCCTGCCAGACCTGTCTCCGGCTGACGCCGTTCAGCCCGAGGC
GAGCTTTCATCCGGTTCACCAGGCGGCCGCGCAACTCGCGCGCGGCGAGACCACCGCGCAGGCGCTGGTCGATGCCTGTC
ACGCGGCCTGGGCCGCCCGCAATGGCGAAATCAATGCAATGGTGCTGGCGGATTTCGAGGCAGCCCGTCACGCCGCCAGG
CAGAGCGATGCGCGCCGCCGGGCCGGGCAGGCGCTTGGACCGCTCGACGGCATTCCGTTCTCGATCAAGGAGTCCTTTGA
CGTGGCTGGCTGGCCGACTACCTGCGGCAGCCCTGCGCGGCGCGCGCATCGGGCCGGCAGCGATGCGGTGGTGGTCGAAC
GGCTGCGTGCCCAGGGCGCCGTGTTGCTCGGCAAGACCAATGTGCCGCTCGGGCTGCGCGACTGGCAGAGCTACAACGCG
ATCTATGGCACCACGCGCAATCCGCACGACCTGTCGCGCACGCCGGGCGGGTCTTCCGGCGGCAGCGCGGCGGCGGTCTG
CGCCGGCATGAGCTACTTCGACATTGGCTCGGATATCGGTTCGTCGCTGCGCAACCCGGCGCACTACTGCGGGGTGTTCT
CGCACAAGAGCAGCCACGGCATCGTGCCACTGCGCGGCCACGGCAACGCAGCGGCAGGGTTTGCCGGGCAGGACATCAAC
GTGGCCGGCCCCGTGGCCCGGAGCGCGTATGACCTCGAACTGATCCTGCGAGCCATCTCCGGCCCCGACGCGGCCGAACT
TCCCGCCTGGAAACTCGATCTGCCGGCGTGCGATCACGCCCGGCTGGCCGATTTTCGCGTGGCCGTGCTGCCCACGCATC
CGTTGGCGGAGGTCGATGCCACGGTGAGCGGCGCGATCGAGGGCCTTGGTCACTGGCTGACCGGGCAGGGCGCGCGCGTG
GACTGGAACGTGCGTCCGGACTTCGATGCGGCGGCGCTTTGGCGCACCTATGTGCTGCTACTGCGTGCCACCACTTCGCT
CTACATGGACGATGCGGCATTCGCCGACGCGCTCGCCAAGGCAGGCGATGCCGGCAACGACGAAGACTACGCCACGCTGC
AGTTCTCTGGCGCCACGCTGAGTCATCGCGACTGGCTATTGCTGCAACACGCTCGCGCCCGGTTCGCGGACGCGTGGGAG
CGATTCTTCGCCGACTACGACGTGCTGCTGTGCCCGGCGGCCTCGACCACGGCGTTCCCGCTCGACGAGGCCGGCGAACC
GTGGCAGCGGACGATCGATGTGAACGGCACGGCATGGCCGCTGACGTCGCAGTTGTTCTGGGCCGGCCATTCGGGGCTGT
GTGGATTGCCGTCCACGGTGGCGCCGATCGGTCCGGCCAGCGATGGGTTACCGGTTGGCGTGCAGATCGTGGCGCGCCGG
TTTGGCGACCTGACATCGCTGCGGTTCGCGCAGCAGCTTGAGGCAGCCGGCCACGTGTTCCGCCAGCCGCGCGCCTGA

Upstream 100 bases:

>100_bases
CCTGGCACAAATACTGCCTTGTGGTCTGTGGTGTTGTGAATATAGTATACCGATTGCTTCTCCAATCGCCCCCACAGACC
GACCCACCGCCCCAAGCCTG

Downstream 100 bases:

>100_bases
CACGCAAGTTCACAACGGTAAAAGGGGAGAAAGACCATGCTGGACTGGTTCAGAGAGTTGTCGCGCGCCGAGCGCAAGGG
GTTCTACGGCGCCTTCCTGG

Product: amidase

Products: monocarboxylate; NH3

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MGSVQDDNGATVTLPDLSPADAVQPEASFHPVHQAAAQLARGETTAQALVDACHAAWAARNGEINAMVLADFEAARHAAR
QSDARRRAGQALGPLDGIPFSIKESFDVAGWPTTCGSPARRAHRAGSDAVVVERLRAQGAVLLGKTNVPLGLRDWQSYNA
IYGTTRNPHDLSRTPGGSSGGSAAAVCAGMSYFDIGSDIGSSLRNPAHYCGVFSHKSSHGIVPLRGHGNAAAGFAGQDIN
VAGPVARSAYDLELILRAISGPDAAELPAWKLDLPACDHARLADFRVAVLPTHPLAEVDATVSGAIEGLGHWLTGQGARV
DWNVRPDFDAAALWRTYVLLLRATTSLYMDDAAFADALAKAGDAGNDEDYATLQFSGATLSHRDWLLLQHARARFADAWE
RFFADYDVLLCPAASTTAFPLDEAGEPWQRTIDVNGTAWPLTSQLFWAGHSGLCGLPSTVAPIGPASDGLPVGVQIVARR
FGDLTSLRFAQQLEAAGHVFRQPRA

Sequences:

>Translated_505_residues
MGSVQDDNGATVTLPDLSPADAVQPEASFHPVHQAAAQLARGETTAQALVDACHAAWAARNGEINAMVLADFEAARHAAR
QSDARRRAGQALGPLDGIPFSIKESFDVAGWPTTCGSPARRAHRAGSDAVVVERLRAQGAVLLGKTNVPLGLRDWQSYNA
IYGTTRNPHDLSRTPGGSSGGSAAAVCAGMSYFDIGSDIGSSLRNPAHYCGVFSHKSSHGIVPLRGHGNAAAGFAGQDIN
VAGPVARSAYDLELILRAISGPDAAELPAWKLDLPACDHARLADFRVAVLPTHPLAEVDATVSGAIEGLGHWLTGQGARV
DWNVRPDFDAAALWRTYVLLLRATTSLYMDDAAFADALAKAGDAGNDEDYATLQFSGATLSHRDWLLLQHARARFADAWE
RFFADYDVLLCPAASTTAFPLDEAGEPWQRTIDVNGTAWPLTSQLFWAGHSGLCGLPSTVAPIGPASDGLPVGVQIVARR
FGDLTSLRFAQQLEAAGHVFRQPRA
>Mature_504_residues
GSVQDDNGATVTLPDLSPADAVQPEASFHPVHQAAAQLARGETTAQALVDACHAAWAARNGEINAMVLADFEAARHAARQ
SDARRRAGQALGPLDGIPFSIKESFDVAGWPTTCGSPARRAHRAGSDAVVVERLRAQGAVLLGKTNVPLGLRDWQSYNAI
YGTTRNPHDLSRTPGGSSGGSAAAVCAGMSYFDIGSDIGSSLRNPAHYCGVFSHKSSHGIVPLRGHGNAAAGFAGQDINV
AGPVARSAYDLELILRAISGPDAAELPAWKLDLPACDHARLADFRVAVLPTHPLAEVDATVSGAIEGLGHWLTGQGARVD
WNVRPDFDAAALWRTYVLLLRATTSLYMDDAAFADALAKAGDAGNDEDYATLQFSGATLSHRDWLLLQHARARFADAWER
FFADYDVLLCPAASTTAFPLDEAGEPWQRTIDVNGTAWPLTSQLFWAGHSGLCGLPSTVAPIGPASDGLPVGVQIVARRF
GDLTSLRFAQQLEAAGHVFRQPRA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=468, Percent_Identity=29.9145299145299, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI222831590, Length=511, Percent_Identity=25.440313111546, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI166795287, Length=155, Percent_Identity=37.4193548387097, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17537465, Length=244, Percent_Identity=36.0655737704918, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17556264, Length=228, Percent_Identity=37.280701754386, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17538252, Length=229, Percent_Identity=33.1877729257642, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71990152, Length=225, Percent_Identity=33.7777777777778, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17543272, Length=443, Percent_Identity=26.1851015801354, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17556276, Length=203, Percent_Identity=38.423645320197, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17556278, Length=203, Percent_Identity=38.423645320197, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17538254, Length=204, Percent_Identity=32.843137254902, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320448, Length=228, Percent_Identity=32.8947368421053, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319685, Length=417, Percent_Identity=23.9808153477218, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21356731, Length=492, Percent_Identity=27.8455284552846, Blast_Score=154, Evalue=9e-38,
Organism=Drosophila melanogaster, GI24644968, Length=515, Percent_Identity=28.1553398058252, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI45550774, Length=242, Percent_Identity=36.3636363636364, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24648435, Length=242, Percent_Identity=36.3636363636364, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24648437, Length=242, Percent_Identity=36.3636363636364, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24648441, Length=186, Percent_Identity=39.247311827957, Blast_Score=129, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24648439, Length=186, Percent_Identity=39.247311827957, Blast_Score=129, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24652985, Length=495, Percent_Identity=26.8686868686869, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI19922090, Length=495, Percent_Identity=26.8686868686869, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24652981, Length=495, Percent_Identity=26.8686868686869, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24652983, Length=495, Percent_Identity=26.8686868686869, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI161078093, Length=491, Percent_Identity=25.8655804480652, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24648113, Length=499, Percent_Identity=28.0561122244489, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 3.5.1.4

Molecular weight: Translated: 53402; Mature: 53271

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSVQDDNGATVTLPDLSPADAVQPEASFHPVHQAAAQLARGETTAQALVDACHAAWAAR
CCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
NGEINAMVLADFEAARHAARQSDARRRAGQALGPLDGIPFSIKESFDVAGWPTTCGSPAR
CCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCCHHH
RAHRAGSDAVVVERLRAQGAVLLGKTNVPLGLRDWQSYNAIYGTTRNPHDLSRTPGGSSG
HHHHCCCCHHHHHHHHHCCEEEEECCCCCCCCHHHHCCCCEECCCCCCCCCCCCCCCCCC
GSAAAVCAGMSYFDIGSDIGSSLRNPAHYCGVFSHKSSHGIVPLRGHGNAAAGFAGQDIN
CCHHHHHHCCHHHHCCHHHHHHHCCCHHHHEEECCCCCCCEEEECCCCCCCCCCCCCCCC
VAGPVARSAYDLELILRAISGPDAAELPAWKLDLPACDHARLADFRVAVLPTHPLAEVDA
CCCCHHCCHHHHHHHHHHHCCCCHHHCCCEEECCCCCCCCCCCCEEEEEECCCCHHHHHH
TVSGAIEGLGHWLTGQGARVDWNVRPDFDAAALWRTYVLLLRATTSLYMDDAAFADALAK
HHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGDAGNDEDYATLQFSGATLSHRDWLLLQHARARFADAWERFFADYDVLLCPAASTTAFP
CCCCCCCCCEEEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
LDEAGEPWQRTIDVNGTAWPLTSQLFWAGHSGLCGLPSTVAPIGPASDGLPVGVQIVARR
CCCCCCCCCEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
FGDLTSLRFAQQLEAAGHVFRQPRA
HCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GSVQDDNGATVTLPDLSPADAVQPEASFHPVHQAAAQLARGETTAQALVDACHAAWAAR
CCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
NGEINAMVLADFEAARHAARQSDARRRAGQALGPLDGIPFSIKESFDVAGWPTTCGSPAR
CCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCCHHH
RAHRAGSDAVVVERLRAQGAVLLGKTNVPLGLRDWQSYNAIYGTTRNPHDLSRTPGGSSG
HHHHCCCCHHHHHHHHHCCEEEEECCCCCCCCHHHHCCCCEECCCCCCCCCCCCCCCCCC
GSAAAVCAGMSYFDIGSDIGSSLRNPAHYCGVFSHKSSHGIVPLRGHGNAAAGFAGQDIN
CCHHHHHHCCHHHHCCHHHHHHHCCCHHHHEEECCCCCCCEEEECCCCCCCCCCCCCCCC
VAGPVARSAYDLELILRAISGPDAAELPAWKLDLPACDHARLADFRVAVLPTHPLAEVDA
CCCCHHCCHHHHHHHHHHHCCCCHHHCCCEEECCCCCCCCCCCCEEEEEECCCCHHHHHH
TVSGAIEGLGHWLTGQGARVDWNVRPDFDAAALWRTYVLLLRATTSLYMDDAAFADALAK
HHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGDAGNDEDYATLQFSGATLSHRDWLLLQHARARFADAWERFFADYDVLLCPAASTTAFP
CCCCCCCCCEEEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
LDEAGEPWQRTIDVNGTAWPLTSQLFWAGHSGLCGLPSTVAPIGPASDGLPVGVQIVARR
CCCCCCCCCEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
FGDLTSLRFAQQLEAAGHVFRQPRA
HCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: monocarboxylic acid amide; H2O

Specific reaction: A monocarboxylic acid amide + H2O = a monocarboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA