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The map label for this gene is glyS [H]

Identifier: 94309391

GI number: 94309391

Start: 471716

End: 473839

Strand: Reverse

Name: glyS [H]

Synonym: Rmet_0446

Alternate gene names: 94309391

Gene position: 473839-471716 (Counterclockwise)

Preceding gene: 94309392

Following gene: 94309390

Centisome position: 12.06

GC content: 67.04

Gene sequence:

>2124_bases
ATGTCGCAACCCATGACCGATTCGCTGCTGATCGAACTGTTCACTGAAGAACTGCCGCCGAAGGCGCTGGCACGCCTGGG
CGAGGCCTTTGCCCAAGGCCTGTTCGACGGCCTCGGCGCGCGCGATCTGCTCGAAGCAGGCGCCACCGTTACGCCTTTCG
CCACGCCGCGCCGCCTGGGCGCGCTGGTCTCCGGCGTGCGCCGCACCGCGCCGGACCGCGAGCAGCGCGAGAAGGTACTG
CCGCTGACCGTGGCGCTCGATGCCAACGGCGCCCCGACGGCTCCGCTGGGCAAGAAGCTGGCGGCACTGGCCAAATCGGT
CGGCGTAGCCGAGATTGACTGGCAGACGCTCGAGCGCGCCTCCGACGGCAAGGCCGATGCGTTCTTCTACCGCTACACCG
CGCGCGGCGCGACGCTGGCCGATGGCCTGCAGGCAGCGCTCGACGAGACGCTGACCAAGCTGCCGATCCCGAAGGTGATG
AGCTACCAGCGTCCGGACGGCACCACCGTGCATTTCGTGCGTCCGGCACACAGCCTGATCGCGCTGTACGGCGCGGAGAT
CGTGCCGGTTTCGGCGCTCGGCCTGCAAGCCAGCAATCTCACGCAAGGTCACCGCTTCCTGTCGCAGGGCACGATCGAGA
TTCCCCACGCGGCCGAGTACGCCGGCCTGCTGGAATCGCGCGGCCGCGTCGTCGCCAGCTATGCCGACCGGCGCGAGCGC
ATCCGCACGGCGCTGCTCAAGGAAGCCGGCGCCGACAAGGTCATCATGCCGGACTCGCTGCTTGACGAGGTGAACTCGCT
GGTCGAATGGCCGGTGATCTACCCGTGCCACTTCGAGGAAGCCTTCCTGGCCGTGCCGCAGGAGTGCCTGATCCTGACGA
TGCAGACCAACCAGAAGTACTTTGCACTGACCGATGCCGAAGGGCACCTGCGCAACCGCTTCCTGATCGTATCGAACCTG
GCCACCGACACCCCGCAATCGATCATCACGGGCAACGAGCGTGTGGTGCGCCCACGCCTGGCCGATGCGAAGTTCTTCTT
CGACCAGGACCGCAAAAAGACGCTGGCCTCGCGCGTGCCGCAACTCGCCAACGTGGTCTACCACAACAAGATCGGCAACC
AGCTCGACCGCGTGCAGCGCCTGCAGCAGATCGCCGGCCATCTTGCCGACGCGATGAACGCCACCGGCGCCCCGGTCGAC
AAGGCCGCCGCGATGCGCGGCGCCGAACTGGCCAAGGCCGACCTGCTGACCGATATGGTCGGCGAGTTCCCCGAGCTGCA
AGGCACGATGGGCACGTACTACGCCCGCCACGACGACGAAGCCGAGGACGTCGCCCTGGCCTGCTCCGAACACTATCGCC
CGCGCTTTGCCGGCGACGCGCTGCCGGCAGGCAACGTGAGCACCGCCGTGGCGCTGGCCGACAAGCTTGAGACGCTGATT
GGCATCTGGGGCATCGGCCTGCAGCCGACGGGCGAAAAGGACCCGTTCGCGCTGCGCCGCCACGCGCTGGGCATCCTGCG
GATGCTGATCGAGAAACCGCTGGCGCTGCCCCTGAACGACGTGCTGGGTCTGACCGAACAAGCCTTCGCCGGCATCGCGG
CGGTCAAGCCTTCGCGCGAAGCGATCACCGACTTCCTCTTCGACCGCGTACGTGGCTACCTGAAGGACAAGGGCTACACG
ACCAACGAAGTAGAAGCCGTGGTCAGCCTGCGCCCGCAGCAACTGCACGACATCCTGGCGCGCATGGAAGCCGTCCGCAC
GTTCGCGGCGTTGCCTGAAGCCGAAGCCCTGGCCGCAGCGAACAAACGCATCACGAACATCCTGAAGAAGACGGACGAGG
CAATCGGCAGCGTCGATGTATCGCTGTTCCAGGAGGCTGCCGAAGGCGCGTTGCACGAGGCGATCGAGCGCGTGCGCCCG
GGCGTGGAAGCCGCGTTCGCGGGTGGTGATTTCACCGCCGCGCTGCGCGACCTGGCACAGCTTCGCGATGCTGTGGACCG
CTTCTTCAACGACGTGATGGTCATGGCCGAGGACGCGAAGCTGCGCGCCAACCGCCTGGCGCTGTTGGCCACGCTGCACG
GCCTGGCGAATCGCGTCGCGGACATTTCGAAGCTGGCGGCCTAA

Upstream 100 bases:

>100_bases
GCAATTCCGCCAGCAGAATGGCCTGTGGCTGCTGTCTGGCGTGGAACCCAATCTCTCGCCGCGCTGCTAAGCGCGCGCCC
ATACCTGTTGGATACGATTC

Downstream 100 bases:

>100_bases
GCTGTCTCATACGCTGCATCACCGGAACGCGCGCCATGCCACAGTCCCAGTTGAAGTTCGTCATCCTGGATCGCGACGGA
GTCATCAACCGCGACAGCGA

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 707; Mature: 706

Protein sequence:

>707_residues
MSQPMTDSLLIELFTEELPPKALARLGEAFAQGLFDGLGARDLLEAGATVTPFATPRRLGALVSGVRRTAPDREQREKVL
PLTVALDANGAPTAPLGKKLAALAKSVGVAEIDWQTLERASDGKADAFFYRYTARGATLADGLQAALDETLTKLPIPKVM
SYQRPDGTTVHFVRPAHSLIALYGAEIVPVSALGLQASNLTQGHRFLSQGTIEIPHAAEYAGLLESRGRVVASYADRRER
IRTALLKEAGADKVIMPDSLLDEVNSLVEWPVIYPCHFEEAFLAVPQECLILTMQTNQKYFALTDAEGHLRNRFLIVSNL
ATDTPQSIITGNERVVRPRLADAKFFFDQDRKKTLASRVPQLANVVYHNKIGNQLDRVQRLQQIAGHLADAMNATGAPVD
KAAAMRGAELAKADLLTDMVGEFPELQGTMGTYYARHDDEAEDVALACSEHYRPRFAGDALPAGNVSTAVALADKLETLI
GIWGIGLQPTGEKDPFALRRHALGILRMLIEKPLALPLNDVLGLTEQAFAGIAAVKPSREAITDFLFDRVRGYLKDKGYT
TNEVEAVVSLRPQQLHDILARMEAVRTFAALPEAEALAAANKRITNILKKTDEAIGSVDVSLFQEAAEGALHEAIERVRP
GVEAAFAGGDFTAALRDLAQLRDAVDRFFNDVMVMAEDAKLRANRLALLATLHGLANRVADISKLAA

Sequences:

>Translated_707_residues
MSQPMTDSLLIELFTEELPPKALARLGEAFAQGLFDGLGARDLLEAGATVTPFATPRRLGALVSGVRRTAPDREQREKVL
PLTVALDANGAPTAPLGKKLAALAKSVGVAEIDWQTLERASDGKADAFFYRYTARGATLADGLQAALDETLTKLPIPKVM
SYQRPDGTTVHFVRPAHSLIALYGAEIVPVSALGLQASNLTQGHRFLSQGTIEIPHAAEYAGLLESRGRVVASYADRRER
IRTALLKEAGADKVIMPDSLLDEVNSLVEWPVIYPCHFEEAFLAVPQECLILTMQTNQKYFALTDAEGHLRNRFLIVSNL
ATDTPQSIITGNERVVRPRLADAKFFFDQDRKKTLASRVPQLANVVYHNKIGNQLDRVQRLQQIAGHLADAMNATGAPVD
KAAAMRGAELAKADLLTDMVGEFPELQGTMGTYYARHDDEAEDVALACSEHYRPRFAGDALPAGNVSTAVALADKLETLI
GIWGIGLQPTGEKDPFALRRHALGILRMLIEKPLALPLNDVLGLTEQAFAGIAAVKPSREAITDFLFDRVRGYLKDKGYT
TNEVEAVVSLRPQQLHDILARMEAVRTFAALPEAEALAAANKRITNILKKTDEAIGSVDVSLFQEAAEGALHEAIERVRP
GVEAAFAGGDFTAALRDLAQLRDAVDRFFNDVMVMAEDAKLRANRLALLATLHGLANRVADISKLAA
>Mature_706_residues
SQPMTDSLLIELFTEELPPKALARLGEAFAQGLFDGLGARDLLEAGATVTPFATPRRLGALVSGVRRTAPDREQREKVLP
LTVALDANGAPTAPLGKKLAALAKSVGVAEIDWQTLERASDGKADAFFYRYTARGATLADGLQAALDETLTKLPIPKVMS
YQRPDGTTVHFVRPAHSLIALYGAEIVPVSALGLQASNLTQGHRFLSQGTIEIPHAAEYAGLLESRGRVVASYADRRERI
RTALLKEAGADKVIMPDSLLDEVNSLVEWPVIYPCHFEEAFLAVPQECLILTMQTNQKYFALTDAEGHLRNRFLIVSNLA
TDTPQSIITGNERVVRPRLADAKFFFDQDRKKTLASRVPQLANVVYHNKIGNQLDRVQRLQQIAGHLADAMNATGAPVDK
AAAMRGAELAKADLLTDMVGEFPELQGTMGTYYARHDDEAEDVALACSEHYRPRFAGDALPAGNVSTAVALADKLETLIG
IWGIGLQPTGEKDPFALRRHALGILRMLIEKPLALPLNDVLGLTEQAFAGIAAVKPSREAITDFLFDRVRGYLKDKGYTT
NEVEAVVSLRPQQLHDILARMEAVRTFAALPEAEALAAANKRITNILKKTDEAIGSVDVSLFQEAAEGALHEAIERVRPG
VEAAFAGGDFTAALRDLAQLRDAVDRFFNDVMVMAEDAKLRANRLALLATLHGLANRVADISKLAA

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=706, Percent_Identity=43.0594900849858, Blast_Score=520, Evalue=1e-149,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 76751; Mature: 76620

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPMTDSLLIELFTEELPPKALARLGEAFAQGLFDGLGARDLLEAGATVTPFATPRRLG
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHH
ALVSGVRRTAPDREQREKVLPLTVALDANGAPTAPLGKKLAALAKSVGVAEIDWQTLERA
HHHHHHHHCCCCHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHC
SDGKADAFFYRYTARGATLADGLQAALDETLTKLPIPKVMSYQRPDGTTVHFVRPAHSLI
CCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCEEEEECHHHHHH
ALYGAEIVPVSALGLQASNLTQGHRFLSQGTIEIPHAAEYAGLLESRGRVVASYADRRER
HHHCCCCEEHHHHCCCHHCCHHHHHHHHCCCEECCCHHHHHHHHHHCCCEEHHHHHHHHH
IRTALLKEAGADKVIMPDSLLDEVNSLVEWPVIYPCHFEEAFLAVPQECLILTMQTNQKY
HHHHHHHHCCCCCEECCHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCEEEEEECCCEE
FALTDAEGHLRNRFLIVSNLATDTPQSIITGNERVVRPRLADAKFFFDQDRKKTLASRVP
EEEECCCCCHHCCEEEEECCCCCCCHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHH
QLANVVYHNKIGNQLDRVQRLQQIAGHLADAMNATGAPVDKAAAMRGAELAKADLLTDMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GEFPELQGTMGTYYARHDDEAEDVALACSEHYRPRFAGDALPAGNVSTAVALADKLETLI
HCCHHHCCCHHHHEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
GIWGIGLQPTGEKDPFALRRHALGILRMLIEKPLALPLNDVLGLTEQAFAGIAAVKPSRE
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHCCCCHH
AITDFLFDRVRGYLKDKGYTTNEVEAVVSLRPQQLHDILARMEAVRTFAALPEAEALAAA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
NKRITNILKKTDEAIGSVDVSLFQEAAEGALHEAIERVRPGVEAAFAGGDFTAALRDLAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
LRDAVDRFFNDVMVMAEDAKLRANRLALLATLHGLANRVADISKLAA
HHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQPMTDSLLIELFTEELPPKALARLGEAFAQGLFDGLGARDLLEAGATVTPFATPRRLG
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHH
ALVSGVRRTAPDREQREKVLPLTVALDANGAPTAPLGKKLAALAKSVGVAEIDWQTLERA
HHHHHHHHCCCCHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHC
SDGKADAFFYRYTARGATLADGLQAALDETLTKLPIPKVMSYQRPDGTTVHFVRPAHSLI
CCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCEEEEECHHHHHH
ALYGAEIVPVSALGLQASNLTQGHRFLSQGTIEIPHAAEYAGLLESRGRVVASYADRRER
HHHCCCCEEHHHHCCCHHCCHHHHHHHHCCCEECCCHHHHHHHHHHCCCEEHHHHHHHHH
IRTALLKEAGADKVIMPDSLLDEVNSLVEWPVIYPCHFEEAFLAVPQECLILTMQTNQKY
HHHHHHHHCCCCCEECCHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCEEEEEECCCEE
FALTDAEGHLRNRFLIVSNLATDTPQSIITGNERVVRPRLADAKFFFDQDRKKTLASRVP
EEEECCCCCHHCCEEEEECCCCCCCHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHH
QLANVVYHNKIGNQLDRVQRLQQIAGHLADAMNATGAPVDKAAAMRGAELAKADLLTDMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GEFPELQGTMGTYYARHDDEAEDVALACSEHYRPRFAGDALPAGNVSTAVALADKLETLI
HCCHHHCCCHHHHEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
GIWGIGLQPTGEKDPFALRRHALGILRMLIEKPLALPLNDVLGLTEQAFAGIAAVKPSRE
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHCCCCHH
AITDFLFDRVRGYLKDKGYTTNEVEAVVSLRPQQLHDILARMEAVRTFAALPEAEALAAA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
NKRITNILKKTDEAIGSVDVSLFQEAAEGALHEAIERVRPGVEAAFAGGDFTAALRDLAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
LRDAVDRFFNDVMVMAEDAKLRANRLALLATLHGLANRVADISKLAA
HHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA