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The map label for this gene is ttuC [H]

Identifier: 94309349

GI number: 94309349

Start: 431492

End: 432490

Strand: Direct

Name: ttuC [H]

Synonym: Rmet_0404

Alternate gene names: 94309349

Gene position: 431492-432490 (Clockwise)

Preceding gene: 94309347

Following gene: 94309350

Centisome position: 10.98

GC content: 63.56

Gene sequence:

>999_bases
ATGGATGCCACCGCACGACGTTTCGGCATCGATTTTCAGTGGGACCACTTCGACTTTTCCAGTTACGCGTACTACGAGCG
CCACGGCAAGATGCTGCCGGACGACTGGTTTGACACCCTGGTCAAGTACGACGCCATCTACTTTGGCGCGGTTGGCTGGC
CGGCGAAGATTGCCGATCACGTGTCGCTGTGGGGCTCCCTGCTGCAGTTCCGGCGCTCGTTCGACCAGTATGTGAACCTG
CGCCCGGTCCGGCTGATGCCCGGCATCAAGAGCCCGCTGGCCGACCGCAAGCCGGGCGACATCGATTTCTACGTGGTGCG
CGAGAACACCGAGGGTGAGTATTCGAGTATCGGTGGGCGCATGTTCCCGGGTACCGAGCGCGAGATCGTGATGCAGGAGA
CGGTGATGAGCCGGCACGGCGTCGACCGCATCCTGAAATTCGCGTTCGAACTGGCGCAGAAACGCCCGAAGAAGCACCTG
ACGTCGGCCACCAAGTCCAACGGGATCTCGATCACGATGCCGTACTGGGACGAACGGGTGGAGGCGATGGCCGCCAACTA
TCCAGGCATGAAGGTCGACAAGTACCACATCGACATCCTGACCGCCCACTTCGTACAGCACCCGGACTGGTTCGACGTAG
TGGTGGCCAGCAACCTGTTCGGCGACATCCTGTCCGATCTCGGCCCGGCCTGTACCGGCACGATCGGCGTGGCGCCATCG
GGCAACATCAATCCTGATAAGGTCTATCCCAGCTTGTTCGAGCCCGTGCATGGCTCGGCGCCCGACATCGCTGGCCAGGG
CATCGCCAATCCGATCGGCCAGATCTGGTGCGGTGCAATGATGCTCGAACACCTGGGCCACGCCGAGGCTGGCGCGGCCG
TGCTGGCGGCCATCGAGCAGGTGCTGGCCGCGGGACCGGGTCATGCGCCGCTGACGCGTGATATTGGCGGTACCGCCTCC
ACGGCCGATCTCGGCCAGGCCATCGCGGAGGCGCTGTGA

Upstream 100 bases:

>100_bases
CCGCAGTCACCCCTCCTTCCCAGCCATGAGTCAATACAGGATCGCTGTCATTCCCGGAGACGGAATCGGAACGGAAGTGA
TGCCCGAAGGCATCCGCGTC

Downstream 100 bases:

>100_bases
ATCCACAGGTCGATCCCCGTACATTGCTGCTGGAGAGCTTCCGCGCCGCCGTGGCGGCAGCCGATCCGCTCGAAATCGTG
GCCGCGCACCTGCCGCCACC

Product: tartrate dehydrogenase/decarboxylase

Products: NA

Alternate protein names: D-malate degradation protein A; D-malate oxidase [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MDATARRFGIDFQWDHFDFSSYAYYERHGKMLPDDWFDTLVKYDAIYFGAVGWPAKIADHVSLWGSLLQFRRSFDQYVNL
RPVRLMPGIKSPLADRKPGDIDFYVVRENTEGEYSSIGGRMFPGTEREIVMQETVMSRHGVDRILKFAFELAQKRPKKHL
TSATKSNGISITMPYWDERVEAMAANYPGMKVDKYHIDILTAHFVQHPDWFDVVVASNLFGDILSDLGPACTGTIGVAPS
GNINPDKVYPSLFEPVHGSAPDIAGQGIANPIGQIWCGAMMLEHLGHAEAGAAVLAAIEQVLAAGPGHAPLTRDIGGTAS
TADLGQAIAEAL

Sequences:

>Translated_332_residues
MDATARRFGIDFQWDHFDFSSYAYYERHGKMLPDDWFDTLVKYDAIYFGAVGWPAKIADHVSLWGSLLQFRRSFDQYVNL
RPVRLMPGIKSPLADRKPGDIDFYVVRENTEGEYSSIGGRMFPGTEREIVMQETVMSRHGVDRILKFAFELAQKRPKKHL
TSATKSNGISITMPYWDERVEAMAANYPGMKVDKYHIDILTAHFVQHPDWFDVVVASNLFGDILSDLGPACTGTIGVAPS
GNINPDKVYPSLFEPVHGSAPDIAGQGIANPIGQIWCGAMMLEHLGHAEAGAAVLAAIEQVLAAGPGHAPLTRDIGGTAS
TADLGQAIAEAL
>Mature_332_residues
MDATARRFGIDFQWDHFDFSSYAYYERHGKMLPDDWFDTLVKYDAIYFGAVGWPAKIADHVSLWGSLLQFRRSFDQYVNL
RPVRLMPGIKSPLADRKPGDIDFYVVRENTEGEYSSIGGRMFPGTEREIVMQETVMSRHGVDRILKFAFELAQKRPKKHL
TSATKSNGISITMPYWDERVEAMAANYPGMKVDKYHIDILTAHFVQHPDWFDVVVASNLFGDILSDLGPACTGTIGVAPS
GNINPDKVYPSLFEPVHGSAPDIAGQGIANPIGQIWCGAMMLEHLGHAEAGAAVLAAIEQVLAAGPGHAPLTRDIGGTAS
TADLGQAIAEAL

Specific function: Catalyzes the NAD(+)-dependent oxidative decarboxylation of D-malate into pyruvate. Is essential for aerobic growth on D- malate as the sole carbon source. But is not required for anaerobic D-malate utilization, although DmlA is expressed and active in th

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=320, Percent_Identity=33.125, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI4758582, Length=259, Percent_Identity=28.957528957529, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI28178838, Length=246, Percent_Identity=30.4878048780488, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI28178816, Length=324, Percent_Identity=25.6172839506173, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI28178821, Length=320, Percent_Identity=25.3125, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI28178819, Length=210, Percent_Identity=28.0952380952381, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1788101, Length=337, Percent_Identity=64.0949554896143, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI87081683, Length=337, Percent_Identity=36.4985163204748, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI1787381, Length=306, Percent_Identity=27.4509803921569, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71986051, Length=255, Percent_Identity=36.4705882352941, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17550882, Length=326, Percent_Identity=30.9815950920245, Blast_Score=151, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI25144293, Length=271, Percent_Identity=28.0442804428044, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17505779, Length=265, Percent_Identity=29.0566037735849, Blast_Score=104, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6322097, Length=333, Percent_Identity=34.8348348348348, Blast_Score=162, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6324709, Length=327, Percent_Identity=30.2752293577982, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6324291, Length=283, Percent_Identity=34.2756183745583, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6319830, Length=334, Percent_Identity=33.2335329341317, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24643268, Length=265, Percent_Identity=33.2075471698113, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24643270, Length=265, Percent_Identity=33.2075471698113, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24661184, Length=271, Percent_Identity=30.9963099630996, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI281362242, Length=298, Percent_Identity=29.1946308724832, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24648872, Length=298, Percent_Identity=29.1946308724832, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161078637, Length=264, Percent_Identity=29.9242424242424, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI161078639, Length=266, Percent_Identity=29.6992481203008, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI161078633, Length=264, Percent_Identity=29.9242424242424, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24650122, Length=264, Percent_Identity=29.9242424242424, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI161078635, Length=264, Percent_Identity=29.9242424242424, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI20130355, Length=265, Percent_Identity=26.4150943396226, Blast_Score=100, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.83 [H]

Molecular weight: Translated: 36530; Mature: 36530

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDATARRFGIDFQWDHFDFSSYAYYERHGKMLPDDWFDTLVKYDAIYFGAVGWPAKIADH
CCCCCHHHCCCEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
VSLWGSLLQFRRSFDQYVNLRPVRLMPGIKSPLADRKPGDIDFYVVRENTEGEYSSIGGR
HHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCEEEEEEECCCCCHHHHCCCC
MFPGTEREIVMQETVMSRHGVDRILKFAFELAQKRPKKHLTSATKSNGISITMPYWDERV
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEECCHHHHH
EAMAANYPGMKVDKYHIDILTAHFVQHPDWFDVVVASNLFGDILSDLGPACTGTIGVAPS
HHHHHCCCCCEEEHEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHCCCEECCCC
GNINPDKVYPSLFEPVHGSAPDIAGQGIANPIGQIWCGAMMLEHLGHAEAGAAVLAAIEQ
CCCCHHHCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VLAAGPGHAPLTRDIGGTASTADLGQAIAEAL
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
>Mature Secondary Structure
MDATARRFGIDFQWDHFDFSSYAYYERHGKMLPDDWFDTLVKYDAIYFGAVGWPAKIADH
CCCCCHHHCCCEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
VSLWGSLLQFRRSFDQYVNLRPVRLMPGIKSPLADRKPGDIDFYVVRENTEGEYSSIGGR
HHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCEEEEEEECCCCCHHHHCCCC
MFPGTEREIVMQETVMSRHGVDRILKFAFELAQKRPKKHLTSATKSNGISITMPYWDERV
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEECCHHHHH
EAMAANYPGMKVDKYHIDILTAHFVQHPDWFDVVVASNLFGDILSDLGPACTGTIGVAPS
HHHHHCCCCCEEEHEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHCCCEECCCC
GNINPDKVYPSLFEPVHGSAPDIAGQGIANPIGQIWCGAMMLEHLGHAEAGAAVLAAIEQ
CCCCHHHCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VLAAGPGHAPLTRDIGGTASTADLGQAIAEAL
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]