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The map label for this gene is gltK [H]

Identifier: 94309346

GI number: 94309346

Start: 428922

End: 429605

Strand: Direct

Name: gltK [H]

Synonym: Rmet_0401

Alternate gene names: 94309346

Gene position: 428922-429605 (Clockwise)

Preceding gene: 94309345

Following gene: 94309347

Centisome position: 10.92

GC content: 59.8

Gene sequence:

>684_bases
ATGGCATACGAATTTGATTTCAGCTCGATCAACCCCTCCACGCTGCATGTGCTGGGCGAAGGGATGCTCGTGTCGGCCAA
GATCACCGTGACCGCGGTGATCGTCGGCATCGTCTGGGGCACGATTCTGGCGATGATGCGCCTGTCGTCGAACAAGCTGC
TGAACTGGTTCGCGCAGGGGTATGTGACCCTCTTCCGCTCCATCCCGCTGGTGATGGTGCTGTTGTGGTTCTTCCTGATC
ATTCCGCAGTTGCTGCAGAAGACGTTCAACCTGTCGGCGGCATCGGACCTGCGCATGACTTCGGCGCTGATCGCGTTCTC
GCTGTTCGAGGCCGCGTATTACTCGGAGATCATCCGCGCCGGTATCCAGAGCGTGTCGCGCGGGCAGATGTTCGCCGCGC
AGGCGATGGGCATGACCTATGGCCAGAGCATGCGGCTGGTGATCCTGCCGCAGGCGTTCCGCAACATGGTGCCGCTGCTG
CTGACGCAGGGCATCATCCTGTTCCAGGATACGTCGCTGGTCTACGTGAGCGCGCTGGCGGACTTCTTCGGCCAGGCGTA
CGGCATTGGCGAACGTGATGGCCGCATCGTCGAGATGCTGCTTTTCGCCGGCCTCATCTACTTCATCATTTGTTTCTCCG
CTTCGCTGCTGGTCAAGCGTTACCAGAAAAAGGTGGCTGTATGA

Upstream 100 bases:

>100_bases
TGTACGCGCTCATCAACTTCACCGTGATGCTGATCATGCGCTGGGTGGAACGTCGCACCCGCGTGCCCGGCTTCATCGGT
GGCAAGTAAGGAGCTGGAAC

Downstream 100 bases:

>100_bases
TCGAAATCAATAACGTTTCCAAGTGGTACGGCGCCTTCCAGGTGCTGACCGACTGCACCACCAAGGTTGCCAAGGGTGAA
GTGGTGGTGGTGTGCGGTCC

Product: glutamate and aspartate ABC transporter membrane protein

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MAYEFDFSSINPSTLHVLGEGMLVSAKITVTAVIVGIVWGTILAMMRLSSNKLLNWFAQGYVTLFRSIPLVMVLLWFFLI
IPQLLQKTFNLSAASDLRMTSALIAFSLFEAAYYSEIIRAGIQSVSRGQMFAAQAMGMTYGQSMRLVILPQAFRNMVPLL
LTQGIILFQDTSLVYVSALADFFGQAYGIGERDGRIVEMLLFAGLIYFIICFSASLLVKRYQKKVAV

Sequences:

>Translated_227_residues
MAYEFDFSSINPSTLHVLGEGMLVSAKITVTAVIVGIVWGTILAMMRLSSNKLLNWFAQGYVTLFRSIPLVMVLLWFFLI
IPQLLQKTFNLSAASDLRMTSALIAFSLFEAAYYSEIIRAGIQSVSRGQMFAAQAMGMTYGQSMRLVILPQAFRNMVPLL
LTQGIILFQDTSLVYVSALADFFGQAYGIGERDGRIVEMLLFAGLIYFIICFSASLLVKRYQKKVAV
>Mature_226_residues
AYEFDFSSINPSTLHVLGEGMLVSAKITVTAVIVGIVWGTILAMMRLSSNKLLNWFAQGYVTLFRSIPLVMVLLWFFLII
PQLLQKTFNLSAASDLRMTSALIAFSLFEAAYYSEIIRAGIQSVSRGQMFAAQAMGMTYGQSMRLVILPQAFRNMVPLLL
TQGIILFQDTSLVYVSALADFFGQAYGIGERDGRIVEMLLFAGLIYFIICFSASLLVKRYQKKVAV

Specific function: Part of the binding-protein-dependent transport system for aspartate/glutamate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786873, Length=224, Percent_Identity=66.0714285714286, Blast_Score=299, Evalue=1e-82,
Organism=Escherichia coli, GI1788226, Length=213, Percent_Identity=34.7417840375587, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI87082239, Length=207, Percent_Identity=31.8840579710145, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1786874, Length=220, Percent_Identity=29.0909090909091, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1787030, Length=227, Percent_Identity=28.1938325991189, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI1788645, Length=206, Percent_Identity=26.6990291262136, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788646, Length=165, Percent_Identity=28.4848484848485, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI48994930, Length=125, Percent_Identity=32.8, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1787087, Length=216, Percent_Identity=27.3148148148148, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787086, Length=211, Percent_Identity=26.5402843601896, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 25278; Mature: 25146

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYEFDFSSINPSTLHVLGEGMLVSAKITVTAVIVGIVWGTILAMMRLSSNKLLNWFAQG
CCEECCCCCCCCHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVTLFRSIPLVMVLLWFFLIIPQLLQKTFNLSAASDLRMTSALIAFSLFEAAYYSEIIRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIQSVSRGQMFAAQAMGMTYGQSMRLVILPQAFRNMVPLLLTQGIILFQDTSLVYVSALA
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHCCEEEEECCHHHHHHHHH
DFFGQAYGIGERDGRIVEMLLFAGLIYFIICFSASLLVKRYQKKVAV
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AYEFDFSSINPSTLHVLGEGMLVSAKITVTAVIVGIVWGTILAMMRLSSNKLLNWFAQG
CEECCCCCCCCHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVTLFRSIPLVMVLLWFFLIIPQLLQKTFNLSAASDLRMTSALIAFSLFEAAYYSEIIRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIQSVSRGQMFAAQAMGMTYGQSMRLVILPQAFRNMVPLLLTQGIILFQDTSLVYVSALA
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHCCEEEEECCHHHHHHHHH
DFFGQAYGIGERDGRIVEMLLFAGLIYFIICFSASLLVKRYQKKVAV
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]