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The map label for this gene is gltJ [H]

Identifier: 94309345

GI number: 94309345

Start: 428182

End: 428910

Strand: Direct

Name: gltJ [H]

Synonym: Rmet_0400

Alternate gene names: 94309345

Gene position: 428182-428910 (Clockwise)

Preceding gene: 94309344

Following gene: 94309346

Centisome position: 10.9

GC content: 62.41

Gene sequence:

>729_bases
ATGGATTACGCTTTTCATTGGGGAGTCTTCCTCGAGCAGGCGGCGGCGAACGAGACCTACCTGGACTGGATGATTTCCGG
TCTCAAGGTGACGATCGCGCTTGGCCTGTCCTCGTGGATCATCGCTCTCGCCATCGGATCGGTGCTGGGCGTGCTGCGCA
CCGTGCCGAACAAGTGGCTGTCGGGGATCGCCGCCACCTACGTCGAGATCTTCCGGAACATTCCGCTGCTGGTGCAGCTT
TTCATCTGGTACTTCGTCGGCCCCGAACTGCTGCCCGGCGGCGAGGCGATCAAGCAGATGAATCCGTTCACCCAGCAGTT
CCTGGCCGCCATGCTCTGTCTGGGCACCTTCACGGCCGCCCGGGTTTGCGAACAGGTGCGCTCGGGTATCAATTCGCTGC
CGCGCGGCCAGAAGAACGCCGGCCTGGCGATGGGTTTCACGCTGCCGCAGACGTATCGCTACGTGCTGCTGCCGATGGCA
TTCCGCGTTATCGTGCCGCCGCTGACTTCCGAGTTTCTGAATATCTTCAAGAACTCGGCCGTGGCGTCGACGATCGGTCT
GCTGGAACTGGCCGCGCAGGGGCGCCAGCTGGTGGACTACACCGCGCGCCCGTATGAGTCGTTCATCGCCGTCACGGTGC
TGTACGCGCTCATCAACTTCACCGTGATGCTGATCATGCGCTGGGTGGAACGTCGCACCCGCGTGCCCGGCTTCATCGGT
GGCAAGTAA

Upstream 100 bases:

>100_bases
TCTTCAAGACTCCGAACGACAAGGCACTCGACTGATCCCCACGATTGGAGCAGTGCGAAACGGAAGGAGCAGTCCTTCCG
TTTCTTTTTGAGGACGCATC

Downstream 100 bases:

>100_bases
GGAGCTGGAACATGGCATACGAATTTGATTTCAGCTCGATCAACCCCTCCACGCTGCATGTGCTGGGCGAAGGGATGCTC
GTGTCGGCCAAGATCACCGT

Product: glutamate and aspartate ABC transporter membrane protein

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MDYAFHWGVFLEQAAANETYLDWMISGLKVTIALGLSSWIIALAIGSVLGVLRTVPNKWLSGIAATYVEIFRNIPLLVQL
FIWYFVGPELLPGGEAIKQMNPFTQQFLAAMLCLGTFTAARVCEQVRSGINSLPRGQKNAGLAMGFTLPQTYRYVLLPMA
FRVIVPPLTSEFLNIFKNSAVASTIGLLELAAQGRQLVDYTARPYESFIAVTVLYALINFTVMLIMRWVERRTRVPGFIG
GK

Sequences:

>Translated_242_residues
MDYAFHWGVFLEQAAANETYLDWMISGLKVTIALGLSSWIIALAIGSVLGVLRTVPNKWLSGIAATYVEIFRNIPLLVQL
FIWYFVGPELLPGGEAIKQMNPFTQQFLAAMLCLGTFTAARVCEQVRSGINSLPRGQKNAGLAMGFTLPQTYRYVLLPMA
FRVIVPPLTSEFLNIFKNSAVASTIGLLELAAQGRQLVDYTARPYESFIAVTVLYALINFTVMLIMRWVERRTRVPGFIG
GK
>Mature_242_residues
MDYAFHWGVFLEQAAANETYLDWMISGLKVTIALGLSSWIIALAIGSVLGVLRTVPNKWLSGIAATYVEIFRNIPLLVQL
FIWYFVGPELLPGGEAIKQMNPFTQQFLAAMLCLGTFTAARVCEQVRSGINSLPRGQKNAGLAMGFTLPQTYRYVLLPMA
FRVIVPPLTSEFLNIFKNSAVASTIGLLELAAQGRQLVDYTARPYESFIAVTVLYALINFTVMLIMRWVERRTRVPGFIG
GK

Specific function: Part of the binding-protein-dependent transport system for aspartate/glutamate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786874, Length=246, Percent_Identity=60.5691056910569, Blast_Score=304, Evalue=3e-84,
Organism=Escherichia coli, GI1787030, Length=215, Percent_Identity=31.6279069767442, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1786873, Length=231, Percent_Identity=26.4069264069264, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788226, Length=195, Percent_Identity=31.2820512820513, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI48994930, Length=128, Percent_Identity=35.9375, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787086, Length=216, Percent_Identity=27.3148148148148, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1788645, Length=209, Percent_Identity=27.7511961722488, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87082239, Length=173, Percent_Identity=24.8554913294798, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788646, Length=214, Percent_Identity=26.6355140186916, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 26906; Mature: 26906

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYAFHWGVFLEQAAANETYLDWMISGLKVTIALGLSSWIIALAIGSVLGVLRTVPNKWL
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGIAATYVEIFRNIPLLVQLFIWYFVGPELLPGGEAIKQMNPFTQQFLAAMLCLGTFTAA
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHH
RVCEQVRSGINSLPRGQKNAGLAMGFTLPQTYRYVLLPMAFRVIVPPLTSEFLNIFKNSA
HHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VASTIGLLELAAQGRQLVDYTARPYESFIAVTVLYALINFTVMLIMRWVERRTRVPGFIG
HHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GK
CC
>Mature Secondary Structure
MDYAFHWGVFLEQAAANETYLDWMISGLKVTIALGLSSWIIALAIGSVLGVLRTVPNKWL
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGIAATYVEIFRNIPLLVQLFIWYFVGPELLPGGEAIKQMNPFTQQFLAAMLCLGTFTAA
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHH
RVCEQVRSGINSLPRGQKNAGLAMGFTLPQTYRYVLLPMAFRVIVPPLTSEFLNIFKNSA
HHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VASTIGLLELAAQGRQLVDYTARPYESFIAVTVLYALINFTVMLIMRWVERRTRVPGFIG
HHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]