Click here to switch to the map view.

The map label for this gene is otsA [H]

Identifier: 94309302

GI number: 94309302

Start: 378242

End: 379636

Strand: Direct

Name: otsA [H]

Synonym: Rmet_0357

Alternate gene names: 94309302

Gene position: 378242-379636 (Clockwise)

Preceding gene: 94309301

Following gene: 94309311

Centisome position: 9.63

GC content: 65.3

Gene sequence:

>1395_bases
ATGGGAAGACTAGTAGCCGTATCGAACCGGGTTGCCGACCCGCGGAATCATGCGGCGGGCGGACTTGCCGTCGCGCTGTC
CGACACGTTGAACAAGACCGGTGGATTGTGGTTCGGCTGGAGCGGCAAGGTAGTGGACGCCAGCCGCGGCGGCACGCCCG
GAGAGGGCGAGCTGCACCAGATCCAGGCCGGCAATGTCACGCTGGCCACGGTGGATCTGAGCCGGGAGGACTTCGAGTCC
TACTACCTGGGCTACAGCAATGGCGTGCTGTGGCCGGTATTCCACTATCGGGTGGACCTGGCCGACTTCGATTCCGGCGG
CAACCTGAATGCCTACCGCCGCGTGAACCAGCTTTTCGCGCGCAAGCTGGCGCCGCTGCTCAAGCCCGACGATGTGATCT
GGATCCACGACTATCACCTGATTCCGCTGGCTGCGGAATTGCGGGCGATCGGTTGCGGCCAGCGCATTGGCTTCTTCCTG
CATATCCCGCTGCCGCCACGGCTGATCCTGGCTACCATTCCGCAGCACGAGTGGCTGATGCGCGCGCTGTTTGCCTATGA
CCTGCTCGGCTTCCAGAGCCACGACGATGTCGAGCACTTCTCCCGCTATGTGCAGGGCGAGGCCGGCGCCGAACCGATGG
GCGAATACCGCTTCCGTGCATTCCATCGCACCGTGCGTGCGCAGGCGTTTCCGATCGGTATCGATGTCGACGAGTTCATC
GAACTGGGCCGCGCCGACGAAGCGCGCGAGACCTACGAGATGATGCGCGGCCAGTACGCGCGACGCAGGCTGCTGCTTGG
CATCGACCGCCTCGATTACTCGAAGGGCCTGCCCCAGCGGCTCAAGGCTTTCCAGACGCTGCTGGCCGAGTACCCCGAGA
ACCGTTCAAGCGCCACGCTGATCCAGATCGCCGCGCCGTCACGGGAATCGGTCGACGCCTATGCGGACCTGCGGCGCGAC
ATGGAAGGCATCACCGGCGCGATCAACGGCGAGTATGGCGAGCTGGACTGGATGCCGGTGCGCTACATCCACCGCTCCAC
CGCGCGTCGCCGCCTGCCGGGCCTGTGCCGTGCGTGCAACGTGGCGCTGGTGACGCCATTGCGCGACGGCATGAACCTCG
TCGCCAAGGAATACATCGCGGCCCAGGACCCGGAGGATCCCGGGGTGCTGGTGCTGTCCCGCTTCGCGGGCGCGGCGGAA
CAGCTCAAGGAAGCGCTGCTGGTCAATCCATACGACACGCGTGCCACCGCGCAGGCGATCCAGCAGGCACTGCATATGTC
GCTGTCAGAGCGCCAGGCGCGTCACCAGAAGCTGCTGGAGCGGATTCGCCAGCAGGATGTGCACTGGTGGAGCGGGGAAT
ATCTGCGGGCGCTAATGGAGACGGAAGGGGGGTGA

Upstream 100 bases:

>100_bases
ACCTATTCGATGGTCGGCCTGATCAATGGCGCCGTACGGCTGTCGGCCCCCTGGGACAGCGTGATTTGAACGAGGAGTGG
ATCTCAACCAGAAGCGAGAT

Downstream 100 bases:

>100_bases
TGGTTGAGGCCGCAGGCAGGCGCCGCTAAATCCGCAGCGCCTACACCTACTTCAGATCGCCCGGCACATCCACATCCCGG
AACGGACCATCGTCGTCGGT

Product: trehalose-6-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MGRLVAVSNRVADPRNHAAGGLAVALSDTLNKTGGLWFGWSGKVVDASRGGTPGEGELHQIQAGNVTLATVDLSREDFES
YYLGYSNGVLWPVFHYRVDLADFDSGGNLNAYRRVNQLFARKLAPLLKPDDVIWIHDYHLIPLAAELRAIGCGQRIGFFL
HIPLPPRLILATIPQHEWLMRALFAYDLLGFQSHDDVEHFSRYVQGEAGAEPMGEYRFRAFHRTVRAQAFPIGIDVDEFI
ELGRADEARETYEMMRGQYARRRLLLGIDRLDYSKGLPQRLKAFQTLLAEYPENRSSATLIQIAAPSRESVDAYADLRRD
MEGITGAINGEYGELDWMPVRYIHRSTARRRLPGLCRACNVALVTPLRDGMNLVAKEYIAAQDPEDPGVLVLSRFAGAAE
QLKEALLVNPYDTRATAQAIQQALHMSLSERQARHQKLLERIRQQDVHWWSGEYLRALMETEGG

Sequences:

>Translated_464_residues
MGRLVAVSNRVADPRNHAAGGLAVALSDTLNKTGGLWFGWSGKVVDASRGGTPGEGELHQIQAGNVTLATVDLSREDFES
YYLGYSNGVLWPVFHYRVDLADFDSGGNLNAYRRVNQLFARKLAPLLKPDDVIWIHDYHLIPLAAELRAIGCGQRIGFFL
HIPLPPRLILATIPQHEWLMRALFAYDLLGFQSHDDVEHFSRYVQGEAGAEPMGEYRFRAFHRTVRAQAFPIGIDVDEFI
ELGRADEARETYEMMRGQYARRRLLLGIDRLDYSKGLPQRLKAFQTLLAEYPENRSSATLIQIAAPSRESVDAYADLRRD
MEGITGAINGEYGELDWMPVRYIHRSTARRRLPGLCRACNVALVTPLRDGMNLVAKEYIAAQDPEDPGVLVLSRFAGAAE
QLKEALLVNPYDTRATAQAIQQALHMSLSERQARHQKLLERIRQQDVHWWSGEYLRALMETEGG
>Mature_463_residues
GRLVAVSNRVADPRNHAAGGLAVALSDTLNKTGGLWFGWSGKVVDASRGGTPGEGELHQIQAGNVTLATVDLSREDFESY
YLGYSNGVLWPVFHYRVDLADFDSGGNLNAYRRVNQLFARKLAPLLKPDDVIWIHDYHLIPLAAELRAIGCGQRIGFFLH
IPLPPRLILATIPQHEWLMRALFAYDLLGFQSHDDVEHFSRYVQGEAGAEPMGEYRFRAFHRTVRAQAFPIGIDVDEFIE
LGRADEARETYEMMRGQYARRRLLLGIDRLDYSKGLPQRLKAFQTLLAEYPENRSSATLIQIAAPSRESVDAYADLRRDM
EGITGAINGEYGELDWMPVRYIHRSTARRRLPGLCRACNVALVTPLRDGMNLVAKEYIAAQDPEDPGVLVLSRFAGAAEQ
LKEALLVNPYDTRATAQAIQQALHMSLSERQARHQKLLERIRQQDVHWWSGEYLRALMETEGG

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=462, Percent_Identity=44.8051948051948, Blast_Score=378, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI115533560, Length=423, Percent_Identity=30.4964539007092, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI115533558, Length=423, Percent_Identity=30.7328605200946, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI71987012, Length=434, Percent_Identity=28.5714285714286, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6319602, Length=477, Percent_Identity=34.5911949685535, Blast_Score=281, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6320279, Length=401, Percent_Identity=27.431421446384, Blast_Score=183, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6323537, Length=390, Percent_Identity=27.9487179487179, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6323917, Length=378, Percent_Identity=28.3068783068783, Blast_Score=167, Evalue=4e-42,
Organism=Drosophila melanogaster, GI19920676, Length=489, Percent_Identity=36.6053169734151, Blast_Score=293, Evalue=1e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 52227; Mature: 52096

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRLVAVSNRVADPRNHAAGGLAVALSDTLNKTGGLWFGWSGKVVDASRGGTPGEGELHQ
CCCEEEECCCCCCCCCCCCCCEEEEEEHHHCCCCCEEECCCCCEEECCCCCCCCCCCEEE
IQAGNVTLATVDLSREDFESYYLGYSNGVLWPVFHYRVDLADFDSGGNLNAYRRVNQLFA
EECCCEEEEEEECCHHHHHHHHEECCCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHHHH
RKLAPLLKPDDVIWIHDYHLIPLAAELRAIGCGQRIGFFLHIPLPPRLILATIPQHEWLM
HHHHHCCCCCCEEEEECCEECHHHHHHHHHCCCCCCCEEEECCCCCCEEEEECCCHHHHH
RALFAYDLLGFQSHDDVEHFSRYVQGEAGAEPMGEYRFRAFHRTVRAQAFPIGIDVDEFI
HHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCHHHHH
ELGRADEARETYEMMRGQYARRRLLLGIDRLDYSKGLPQRLKAFQTLLAEYPENRSSATL
HCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEE
IQIAAPSRESVDAYADLRRDMEGITGAINGEYGELDWMPVRYIHRSTARRRLPGLCRACN
EEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCC
VALVTPLRDGMNLVAKEYIAAQDPEDPGVLVLSRFAGAAEQLKEALLVNPYDTRATAQAI
EEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEHHHCCHHHHHHHHHCCCCCCHHHHHHHH
QQALHMSLSERQARHQKLLERIRQQDVHWWSGEYLRALMETEGG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
GRLVAVSNRVADPRNHAAGGLAVALSDTLNKTGGLWFGWSGKVVDASRGGTPGEGELHQ
CCEEEECCCCCCCCCCCCCCEEEEEEHHHCCCCCEEECCCCCEEECCCCCCCCCCCEEE
IQAGNVTLATVDLSREDFESYYLGYSNGVLWPVFHYRVDLADFDSGGNLNAYRRVNQLFA
EECCCEEEEEEECCHHHHHHHHEECCCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHHHH
RKLAPLLKPDDVIWIHDYHLIPLAAELRAIGCGQRIGFFLHIPLPPRLILATIPQHEWLM
HHHHHCCCCCCEEEEECCEECHHHHHHHHHCCCCCCCEEEECCCCCCEEEEECCCHHHHH
RALFAYDLLGFQSHDDVEHFSRYVQGEAGAEPMGEYRFRAFHRTVRAQAFPIGIDVDEFI
HHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCHHHHH
ELGRADEARETYEMMRGQYARRRLLLGIDRLDYSKGLPQRLKAFQTLLAEYPENRSSATL
HCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEE
IQIAAPSRESVDAYADLRRDMEGITGAINGEYGELDWMPVRYIHRSTARRRLPGLCRACN
EEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCC
VALVTPLRDGMNLVAKEYIAAQDPEDPGVLVLSRFAGAAEQLKEALLVNPYDTRATAQAI
EEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEHHHCCHHHHHHHHHCCCCCCHHHHHHHH
QQALHMSLSERQARHQKLLERIRQQDVHWWSGEYLRALMETEGG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]