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The map label for this gene is moeB [H]

Identifier: 94309194

GI number: 94309194

Start: 265152

End: 265904

Strand: Reverse

Name: moeB [H]

Synonym: Rmet_0249

Alternate gene names: 94309194

Gene position: 265904-265152 (Counterclockwise)

Preceding gene: 94309195

Following gene: 335055481

Centisome position: 6.77

GC content: 67.07

Gene sequence:

>753_bases
ATGAACGACGATCAGCTTCTGCGCTACTCGCGCCATATCCTGCTTGACGAGCTCGGCATCGAAGGCCAGGAGCGCCTGCT
GGCCGGCCATGCGCTGGTGATCGGCGCAGGCGGGCTAGGTGCAGCGGCGTTGCCCTACCTGGCCTCGGCCGGCGTGGGCA
CGATCACGATCGTCGACGACGATGACGTTGACCTGACCAACCTGCAGCGCCAGATCCTGCACACCACCGCCAACGTGGGC
CGCCCCAAGGTCGAATCGGCCAGGGAGGGAATGCTGCGCATCAACCCCGACATCGACATCCGCATGGTGCCGATGCGGGT
GGGCGACGCCGAACTGGATGGTCTCGTCGCGCAGGCTGATGTAGTGCTCGATTGCTGCGACAACTTCGCCACACGCCACG
CGGTGAATCGCGCCTGCCTGCGTCACCGCAAGCCGCTGGTATCCGGCGCCGCGCTACGTTTCGACGGCCAGGTCACCGTA
TTCGACCTGCGCAATCCCGATGCGCCGTGCTACGCCTGCCTGTTCCCGCCATCCGAACCGGCGCCTGAGGCCGCCTGCGC
CACGATGGGCGTGTTCGCGCCGCTGGTCGGCATGATCGGCACGGTACAGGCCGCCGAGGCCCTGAAGTTGCTGACTGATG
TAGGAGAGACGCTGGCGGGCCGCTTGCTGATGGTCGACGCCATGTCGATGGAGTGGACCACGATGCGCCTCGTGCGCACG
CCGGGCTGCGAGGTCTGCGGCGGGGACCACTGA

Upstream 100 bases:

>100_bases
GCGCGACACTGGCGCGTGGCGCTTCCCGCCTTCAGCCCTGCGGCCCGCGGTATTGCACCGGCGGGCCGTTTTTGTTTGAT
CTCCTGATTCCACGTGGACC

Downstream 100 bases:

>100_bases
GTCCCCCCGGGCTGCGGACGATGGACGATCCGGCGCTGGAGCCCACCGGCGCGGACCGTCCATGCTGATACAGCTGGATG
AGTACAAGAATCCGAATGAG

Product: molybdopterin synthase sulfurylase

Products: NA

Alternate protein names: MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MNDDQLLRYSRHILLDELGIEGQERLLAGHALVIGAGGLGAAALPYLASAGVGTITIVDDDDVDLTNLQRQILHTTANVG
RPKVESAREGMLRINPDIDIRMVPMRVGDAELDGLVAQADVVLDCCDNFATRHAVNRACLRHRKPLVSGAALRFDGQVTV
FDLRNPDAPCYACLFPPSEPAPEAACATMGVFAPLVGMIGTVQAAEALKLLTDVGETLAGRLLMVDAMSMEWTTMRLVRT
PGCEVCGGDH

Sequences:

>Translated_250_residues
MNDDQLLRYSRHILLDELGIEGQERLLAGHALVIGAGGLGAAALPYLASAGVGTITIVDDDDVDLTNLQRQILHTTANVG
RPKVESAREGMLRINPDIDIRMVPMRVGDAELDGLVAQADVVLDCCDNFATRHAVNRACLRHRKPLVSGAALRFDGQVTV
FDLRNPDAPCYACLFPPSEPAPEAACATMGVFAPLVGMIGTVQAAEALKLLTDVGETLAGRLLMVDAMSMEWTTMRLVRT
PGCEVCGGDH
>Mature_250_residues
MNDDQLLRYSRHILLDELGIEGQERLLAGHALVIGAGGLGAAALPYLASAGVGTITIVDDDDVDLTNLQRQILHTTANVG
RPKVESAREGMLRINPDIDIRMVPMRVGDAELDGLVAQADVVLDCCDNFATRHAVNRACLRHRKPLVSGAALRFDGQVTV
FDLRNPDAPCYACLFPPSEPAPEAACATMGVFAPLVGMIGTVQAAEALKLLTDVGETLAGRLLMVDAMSMEWTTMRLVRT
PGCEVCGGDH

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein moaD [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=229, Percent_Identity=42.3580786026201, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI4885649, Length=157, Percent_Identity=32.484076433121, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI38045948, Length=153, Percent_Identity=32.0261437908497, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI23510338, Length=152, Percent_Identity=30.9210526315789, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI23510340, Length=152, Percent_Identity=30.9210526315789, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1787048, Length=249, Percent_Identity=50.2008032128514, Blast_Score=243, Evalue=7e-66,
Organism=Escherichia coli, GI87082356, Length=251, Percent_Identity=47.808764940239, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1789177, Length=138, Percent_Identity=36.231884057971, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17540406, Length=258, Percent_Identity=41.0852713178295, Blast_Score=199, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI193203301, Length=167, Percent_Identity=30.5389221556886, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI86565433, Length=147, Percent_Identity=31.2925170068027, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI86565431, Length=147, Percent_Identity=31.2925170068027, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6321903, Length=253, Percent_Identity=36.3636363636364, Blast_Score=162, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6322639, Length=164, Percent_Identity=28.6585365853659, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320598, Length=143, Percent_Identity=28.6713286713287, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24582879, Length=251, Percent_Identity=41.0358565737052, Blast_Score=191, Evalue=3e-49,
Organism=Drosophila melanogaster, GI24660640, Length=173, Percent_Identity=35.2601156069364, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28573937, Length=162, Percent_Identity=30.8641975308642, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24641311, Length=258, Percent_Identity=29.8449612403101, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR012730
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 26713; Mature: 26713

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDDQLLRYSRHILLDELGIEGQERLLAGHALVIGAGGLGAAALPYLASAGVGTITIVDD
CCCHHHHHHHHHHHHHHHCCCCHHHHEECCEEEEECCCCCHHHHHHHHHCCCCEEEEEEC
DDVDLTNLQRQILHTTANVGRPKVESAREGMLRINPDIDIRMVPMRVGDAELDGLVAQAD
CCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCEEECCCCCEEEEEEEECCCHHHHHHHHHH
VVLDCCDNFATRHAVNRACLRHRKPLVSGAALRFDGQVTVFDLRNPDAPCYACLFPPSEP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCCCEEEEECCCCCC
APEAACATMGVFAPLVGMIGTVQAAEALKLLTDVGETLAGRLLMVDAMSMEWTTMRLVRT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEEEC
PGCEVCGGDH
CCCCCCCCCC
>Mature Secondary Structure
MNDDQLLRYSRHILLDELGIEGQERLLAGHALVIGAGGLGAAALPYLASAGVGTITIVDD
CCCHHHHHHHHHHHHHHHCCCCHHHHEECCEEEEECCCCCHHHHHHHHHCCCCEEEEEEC
DDVDLTNLQRQILHTTANVGRPKVESAREGMLRINPDIDIRMVPMRVGDAELDGLVAQAD
CCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCEEECCCCCEEEEEEEECCCHHHHHHHHHH
VVLDCCDNFATRHAVNRACLRHRKPLVSGAALRFDGQVTVFDLRNPDAPCYACLFPPSEP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEEEECCCCCCCEEEEECCCCCC
APEAACATMGVFAPLVGMIGTVQAAEALKLLTDVGETLAGRLLMVDAMSMEWTTMRLVRT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEEEC
PGCEVCGGDH
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3045084; 8905232; 9278503 [H]