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The map label for this gene is pcrA [H]

Identifier: 94309177

GI number: 94309177

Start: 248112

End: 250220

Strand: Reverse

Name: pcrA [H]

Synonym: Rmet_0232

Alternate gene names: 94309177

Gene position: 250220-248112 (Counterclockwise)

Preceding gene: 94309178

Following gene: 94309172

Centisome position: 6.37

GC content: 67.28

Gene sequence:

>2109_bases
GTGTTACCGGAACTTGCCCCTGAAGCCGCTGCCGAGTCGCCCGACACCGAGGTCACCGACGCCCCCGCCTATCTTTCCCG
GCTGAATCCGGAACAGCGCGCGGCGGTGGAGTACGACGGTGACGCGCCGCTGCTGATCATTGCCGGCGCGGGCTCTGGCA
AGACCAACACGCTCGCGCATCGGGTGGCGCATCTGGTCGTGAACGGCGCAGATCCGCGCCGCATCCTGCTGCTGACGTTC
TCGCGCCGCGCCGCCTCCGAAATGGGCCGCCGTGTCGAGCGCATCGTCGACCAGGCACTCGGCATCCAGACCGGCGCGGG
GCGCGCCGCGCTGCAGTGGTCAGGCACGTTCCACGCCATCGGCGCCCGCCTGCTGCGCGAGTACGCCGAAACGCTCGGGC
TTGCTCCGACCTTCACCATCAGCGACCGCGGCGATGCGGCCGACCTGATGCACGTGGTACGCCATGACCTCGGACTATCC
GAGCAGACCTCACGCTTTCCGCGCAAGGAAACCTGCCTCGCGATCTATTCACGCGTGGTCAACACGCAGTTGCCGCTCGA
GGTTGTGCTGAAGACGCAGTTCCCGCGCTACGCGATGTGGGCGGACGCGCTCAAGGGCCTGTTCGCCGCCTACGTGGAAG
CCAAGCAGAAGCAGCAGGTGCTCGACTACGACGACCTGCTGCTCTACTGGGCGCAGGCGATGACCGAGCCCGCACTGGCG
CAGGACATGGGCGCGCGCTTCGACCACGTGCTGGTCGACGAGTACCAGGACACCAACGCGTTGCAGGCGTCGATCCTGCT
GGCGCTCAAGCCGCAAGGGCGCGGCCTGACCGTGGTGGGCGATGACGCGCAGTCGATCTACGCATTCCGCGGCGCCACCG
TGCGCAATATCCTCGATTTCCCGACACAGTTCTCGCCGCCGGCCGAGCAGGTCACGCTGTCGCAGAACTATCGCTCGACG
CAGCCGATCCTCGGCGCCGCCAATGCCGTGATTGGCCTTGCGGCCGAACGCTTCACCAAGGATCTCTGGTCCCTGCGCGA
ATCGGCGGAGAAGCCCGGCGTGGTGGTGGTCAACGACGAGGCCGAGCAGGCGCGCTTCGTCGTCGAGCAGGTACTGGCGC
GGCGCGAGGCTGGCTTGTCGCTGACGCAGCAGGCGGTGCTGTTCCGCGCGGCCGACCACAGCGCCCAGGTCGAGATCGAG
TTGGCCCGGCGCAATATCCCGTTCGTGAAGTTCGGCGGACTGAAGTTTCTGGAGTCGACGCACGTCAAGGACGTGATGGC
CGTGGTGCGCTGGCTGGAAAATCCACGCGACCGCATGGCCGGATTCCGCACGCTGCAGTTGCTGCCCGGCATCGGCCCGA
AAACGGCCGCGCGCGTGCTCGAAGGACTGGAAGCCGCCACCGAACCGTTGATCGCGCTCGAAGCCTTCGAGCCGCCCCCC
GCCGCCGCGCCCGCCTGGGATGAACTGGTGACGCTGGCGCGTGCGCTGATGGCGCCGACCTCGCCCTGGCCATCTGAATT
CGAACAGGTGATCGCCTGGTACATGCCGCACCTCGAACGGATGCACGACGATGCCGCCGCGCGGCAGGCGGACCTGCAGC
AGATCGAGCGCATCGCGGCCACCTATGCGTCGCGGGAACGCTTCCTCACCGAACTCACGCTCGACCCGCCCAGCGCGTCG
AGCGACGAATCCGGCGTGCCCTCGCGCGACGAGGACTACCTGATTCTGTCGACGATCCATTCGGCCAAGGGGCAGGAGTG
GAAGGCCGTGTACGTGCTCAATGCGGTCGATGGCTGCATGCCATCCGATCTTGCCACCGGCACCACCGAGGAAATCGAGG
AAGAGCGCCGCCTGCTGTACGTGGCGATGACGCGCGCGCGCGACCACCTCGACATCGTCGTGCCACAACGCTTCTACGTG
CACAACCAGGTCGGCTTTGGCGATCGCCACGTCTACGCATCGCGCACGCGCTTCCTGCCGAACCGCGTGATGCCGAATTT
CTACAGCCGCTCGTGGCCGCCCGCGCCGATGCCGGGGGAAGGCCAGCAGGCGAAGGCCTCGCTACCGCAGGTAGACCTCG
CCAGCCGAATGCGCGGCATGTGGAAGTAG

Upstream 100 bases:

>100_bases
GCTCACATCGTCGGGCATCCGGCTTGCCTGTCAGGGCTGTATGGATATACAGTGAGCGCCAGATTTTTCTCATCGCATCC
ATCCGACTGCCTGCCCTGAC

Downstream 100 bases:

>100_bases
GAAACCGCTAGTTACCGAAAGTTACCGCCAGAGTTCGTCAGGCCAGTTCGATGCCCGCGCCACGCACGACCTCAACCAGC
GCGCCCTGACGGAGCAGCGC

Product: putative ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 702; Mature: 702

Protein sequence:

>702_residues
MLPELAPEAAAESPDTEVTDAPAYLSRLNPEQRAAVEYDGDAPLLIIAGAGSGKTNTLAHRVAHLVVNGADPRRILLLTF
SRRAASEMGRRVERIVDQALGIQTGAGRAALQWSGTFHAIGARLLREYAETLGLAPTFTISDRGDAADLMHVVRHDLGLS
EQTSRFPRKETCLAIYSRVVNTQLPLEVVLKTQFPRYAMWADALKGLFAAYVEAKQKQQVLDYDDLLLYWAQAMTEPALA
QDMGARFDHVLVDEYQDTNALQASILLALKPQGRGLTVVGDDAQSIYAFRGATVRNILDFPTQFSPPAEQVTLSQNYRST
QPILGAANAVIGLAAERFTKDLWSLRESAEKPGVVVVNDEAEQARFVVEQVLARREAGLSLTQQAVLFRAADHSAQVEIE
LARRNIPFVKFGGLKFLESTHVKDVMAVVRWLENPRDRMAGFRTLQLLPGIGPKTAARVLEGLEAATEPLIALEAFEPPP
AAAPAWDELVTLARALMAPTSPWPSEFEQVIAWYMPHLERMHDDAAARQADLQQIERIAATYASRERFLTELTLDPPSAS
SDESGVPSRDEDYLILSTIHSAKGQEWKAVYVLNAVDGCMPSDLATGTTEEIEEERRLLYVAMTRARDHLDIVVPQRFYV
HNQVGFGDRHVYASRTRFLPNRVMPNFYSRSWPPAPMPGEGQQAKASLPQVDLASRMRGMWK

Sequences:

>Translated_702_residues
MLPELAPEAAAESPDTEVTDAPAYLSRLNPEQRAAVEYDGDAPLLIIAGAGSGKTNTLAHRVAHLVVNGADPRRILLLTF
SRRAASEMGRRVERIVDQALGIQTGAGRAALQWSGTFHAIGARLLREYAETLGLAPTFTISDRGDAADLMHVVRHDLGLS
EQTSRFPRKETCLAIYSRVVNTQLPLEVVLKTQFPRYAMWADALKGLFAAYVEAKQKQQVLDYDDLLLYWAQAMTEPALA
QDMGARFDHVLVDEYQDTNALQASILLALKPQGRGLTVVGDDAQSIYAFRGATVRNILDFPTQFSPPAEQVTLSQNYRST
QPILGAANAVIGLAAERFTKDLWSLRESAEKPGVVVVNDEAEQARFVVEQVLARREAGLSLTQQAVLFRAADHSAQVEIE
LARRNIPFVKFGGLKFLESTHVKDVMAVVRWLENPRDRMAGFRTLQLLPGIGPKTAARVLEGLEAATEPLIALEAFEPPP
AAAPAWDELVTLARALMAPTSPWPSEFEQVIAWYMPHLERMHDDAAARQADLQQIERIAATYASRERFLTELTLDPPSAS
SDESGVPSRDEDYLILSTIHSAKGQEWKAVYVLNAVDGCMPSDLATGTTEEIEEERRLLYVAMTRARDHLDIVVPQRFYV
HNQVGFGDRHVYASRTRFLPNRVMPNFYSRSWPPAPMPGEGQQAKASLPQVDLASRMRGMWK
>Mature_702_residues
MLPELAPEAAAESPDTEVTDAPAYLSRLNPEQRAAVEYDGDAPLLIIAGAGSGKTNTLAHRVAHLVVNGADPRRILLLTF
SRRAASEMGRRVERIVDQALGIQTGAGRAALQWSGTFHAIGARLLREYAETLGLAPTFTISDRGDAADLMHVVRHDLGLS
EQTSRFPRKETCLAIYSRVVNTQLPLEVVLKTQFPRYAMWADALKGLFAAYVEAKQKQQVLDYDDLLLYWAQAMTEPALA
QDMGARFDHVLVDEYQDTNALQASILLALKPQGRGLTVVGDDAQSIYAFRGATVRNILDFPTQFSPPAEQVTLSQNYRST
QPILGAANAVIGLAAERFTKDLWSLRESAEKPGVVVVNDEAEQARFVVEQVLARREAGLSLTQQAVLFRAADHSAQVEIE
LARRNIPFVKFGGLKFLESTHVKDVMAVVRWLENPRDRMAGFRTLQLLPGIGPKTAARVLEGLEAATEPLIALEAFEPPP
AAAPAWDELVTLARALMAPTSPWPSEFEQVIAWYMPHLERMHDDAAARQADLQQIERIAATYASRERFLTELTLDPPSAS
SDESGVPSRDEDYLILSTIHSAKGQEWKAVYVLNAVDGCMPSDLATGTTEEIEEERRLLYVAMTRARDHLDIVVPQRFYV
HNQVGFGDRHVYASRTRFLPNRVMPNFYSRSWPPAPMPGEGQQAKASLPQVDLASRMRGMWK

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=666, Percent_Identity=31.2312312312312, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI48994965, Length=637, Percent_Identity=28.1004709576138, Blast_Score=185, Evalue=7e-48,
Organism=Escherichia coli, GI1787196, Length=376, Percent_Identity=27.6595744680851, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6322369, Length=472, Percent_Identity=27.7542372881356, Blast_Score=151, Evalue=4e-37,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77738; Mature: 77738

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPELAPEAAAESPDTEVTDAPAYLSRLNPEQRAAVEYDGDAPLLIIAGAGSGKTNTLAH
CCCCCCCHHHCCCCCCCCCCCHHHHHHCCHHHHCEEEECCCCCEEEEECCCCCCHHHHHH
RVAHLVVNGADPRRILLLTFSRRAASEMGRRVERIVDQALGIQTGAGRAALQWSGTFHAI
HHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHH
GARLLREYAETLGLAPTFTISDRGDAADLMHVVRHDLGLSEQTSRFPRKETCLAIYSRVV
HHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHH
NTQLPLEVVLKTQFPRYAMWADALKGLFAAYVEAKQKQQVLDYDDLLLYWAQAMTEPALA
CCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHH
QDMGARFDHVLVDEYQDTNALQASILLALKPQGRGLTVVGDDAQSIYAFRGATVRNILDF
HHHCCCCCHHHHHCCCCCCHHEEEEEEEECCCCCEEEEECCCHHHHHHHCCCHHHHHHHC
PTQFSPPAEQVTLSQNYRSTQPILGAANAVIGLAAERFTKDLWSLRESAEKPGVVVVNDE
CCCCCCCHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCC
AEQARFVVEQVLARREAGLSLTQQAVLFRAADHSAQVEIELARRNIPFVKFGGLKFLEST
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEEHHCCCCEEEECCCHHHHHH
HVKDVMAVVRWLENPRDRMAGFRTLQLLPGIGPKTAARVLEGLEAATEPLIALEAFEPPP
HHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCC
AAAPAWDELVTLARALMAPTSPWPSEFEQVIAWYMPHLERMHDDAAARQADLQQIERIAA
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYASRERFLTELTLDPPSASSDESGVPSRDEDYLILSTIHSAKGQEWKAVYVLNAVDGCM
HHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCCCEEEEEEEECCCCCC
PSDLATGTTEEIEEERRLLYVAMTRARDHLDIVVPQRFYVHNQVGFGDRHVYASRTRFLP
CCCCCCCCHHHHHHHHHEEEEEHHHCCCCEEEEECCEEEEECCCCCCCCEEHHHHHHHCC
NRVMPNFYSRSWPPAPMPGEGQQAKASLPQVDLASRMRGMWK
HHCCCCHHCCCCCCCCCCCCCCHHHCCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MLPELAPEAAAESPDTEVTDAPAYLSRLNPEQRAAVEYDGDAPLLIIAGAGSGKTNTLAH
CCCCCCCHHHCCCCCCCCCCCHHHHHHCCHHHHCEEEECCCCCEEEEECCCCCCHHHHHH
RVAHLVVNGADPRRILLLTFSRRAASEMGRRVERIVDQALGIQTGAGRAALQWSGTFHAI
HHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHH
GARLLREYAETLGLAPTFTISDRGDAADLMHVVRHDLGLSEQTSRFPRKETCLAIYSRVV
HHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHH
NTQLPLEVVLKTQFPRYAMWADALKGLFAAYVEAKQKQQVLDYDDLLLYWAQAMTEPALA
CCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHH
QDMGARFDHVLVDEYQDTNALQASILLALKPQGRGLTVVGDDAQSIYAFRGATVRNILDF
HHHCCCCCHHHHHCCCCCCHHEEEEEEEECCCCCEEEEECCCHHHHHHHCCCHHHHHHHC
PTQFSPPAEQVTLSQNYRSTQPILGAANAVIGLAAERFTKDLWSLRESAEKPGVVVVNDE
CCCCCCCHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCC
AEQARFVVEQVLARREAGLSLTQQAVLFRAADHSAQVEIELARRNIPFVKFGGLKFLEST
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEEHHCCCCEEEECCCHHHHHH
HVKDVMAVVRWLENPRDRMAGFRTLQLLPGIGPKTAARVLEGLEAATEPLIALEAFEPPP
HHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCC
AAAPAWDELVTLARALMAPTSPWPSEFEQVIAWYMPHLERMHDDAAARQADLQQIERIAA
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYASRERFLTELTLDPPSASSDESGVPSRDEDYLILSTIHSAKGQEWKAVYVLNAVDGCM
HHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCCCEEEEEEEECCCCCC
PSDLATGTTEEIEEERRLLYVAMTRARDHLDIVVPQRFYVHNQVGFGDRHVYASRTRFLP
CCCCCCCCHHHHHHHHHEEEEEHHHCCCCEEEEECCEEEEECCCCCCCCEEHHHHHHHCC
NRVMPNFYSRSWPPAPMPGEGQQAKASLPQVDLASRMRGMWK
HHCCCCHHCCCCCCCCCCCCCCHHHCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA