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The map label for this gene is rlmI [H]

Identifier: 94309168

GI number: 94309168

Start: 237241

End: 238434

Strand: Reverse

Name: rlmI [H]

Synonym: Rmet_0223

Alternate gene names: 94309168

Gene position: 238434-237241 (Counterclockwise)

Preceding gene: 94309169

Following gene: 94309167

Centisome position: 6.07

GC content: 67.17

Gene sequence:

>1194_bases
ATGAACGTCATCACCCTCAAGCCGGGCAAGGAAAAGTCCCTGCTCCGGCGCCATCCGTGGGTCTACGCCACGGGCATCGC
CGCTACCGAAGGCCGTTGCGAACCGGGATCGACCGTGGTCATCCGCAGCGCGGACGGCCGCTTCCTGGCGCGCGGCGCGT
ACAGCCCCGAATCACAGATCCGCGCGCGTGTCTGGACGTTCGACGAGAACGAGCCGGTCGATCACGCGATGTTCAAGCGC
CGCGTGTCGGCGGCCCTGGCACACCGCAAGCAATGGGTGCGCGACACCAACGCCGTGCGCCTGATCTTCGGCGAATCCGA
CCGCCTGCCGGGTCTGATCGTCGACTACTACGGCCAGGGCGAACGCGGCCAGCTCGTCTGCCAGTTCAACGCCGCGGGCG
TGGAGCAATGGAAAGACGCGCTGGTGCAGGCGCTGATCAAGGAAACCGGCTGCCCGAACGTCTACGAACGTTCTGACGCG
GCGGTGCGCCAGCGCGAAGGACTGCCGCTGGTGACCGGCGTGCTGGCCGGCGCCGAGCCCGACCCGGAACTGTCGGTGAC
CGAGCACGGCGTGCGCTACCACGTCGACGTGCGCAACGGCCACAAGACCGGCTTCTACGTGGACCAGCGCGACAACCGCA
AGCTCGTCGGCGACCTCGCCGAGGGCCGCGAGGTGCTGAACTGCTTCTGCTACACCGGCGGCTTCTCGCTGGCGGCGCTG
CGTGGCGGCGCCACGTCGGTGACGTCGATCGATTCGTCGGGCGAGGCGCTCAAGATCGCGGCCAGCAACGTGACTCTGAA
CGGTTTCGATCCGGCGCGCGCCACGTGGCTCGACGCGGATGTCTTCAAGACGCTGCGTGAATTCCGCGCCGAGGGCCGTC
AGTTCGACCTGATCGTGCTGGACCCTCCCAAGTTCGCGCCGTCGGCACAGCATATCGACCGTGCTGCCCGCGCTTACAAG
GAAATCAACCTGGTCGGGATGCAGTTGCTGCGTCCGGGCGGCCTGCTGTTCACGTACTCGTGCTCGGGTGCGATCAGCAT
GGAGCTGTTCCAGAAGATCGTGGCGGGCGCGGCCACCGATGCGCGCTCGGATGCGCGGATCCTGCGCCGCCTGTCGGCCG
GCACGGACCATCCGATGTCGGCGGCCTTCCCGGAAGGCGAATATCTGAAGGGTCTGCTGCTCGAGAAGATCTGA

Upstream 100 bases:

>100_bases
GGGCTGACGGTGACCGTGTGGGTCACGAGCCGCGAGCAACTCGACGAGCTGTATCGCGCGCTGACCGGGCATCCGATGGT
CAAGGTGGTGCTGTAACCGC

Downstream 100 bases:

>100_bases
CCGGAATCAGGGACGGGCCGAGAATCGCCAGGAAAGGCCAGACTCAGGGTTTTCCGGAATTGTCCTCGGCCTGACTTCTT
GCGGTCACAAACAGCTACTA

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MNVITLKPGKEKSLLRRHPWVYATGIAATEGRCEPGSTVVIRSADGRFLARGAYSPESQIRARVWTFDENEPVDHAMFKR
RVSAALAHRKQWVRDTNAVRLIFGESDRLPGLIVDYYGQGERGQLVCQFNAAGVEQWKDALVQALIKETGCPNVYERSDA
AVRQREGLPLVTGVLAGAEPDPELSVTEHGVRYHVDVRNGHKTGFYVDQRDNRKLVGDLAEGREVLNCFCYTGGFSLAAL
RGGATSVTSIDSSGEALKIAASNVTLNGFDPARATWLDADVFKTLREFRAEGRQFDLIVLDPPKFAPSAQHIDRAARAYK
EINLVGMQLLRPGGLLFTYSCSGAISMELFQKIVAGAATDARSDARILRRLSAGTDHPMSAAFPEGEYLKGLLLEKI

Sequences:

>Translated_397_residues
MNVITLKPGKEKSLLRRHPWVYATGIAATEGRCEPGSTVVIRSADGRFLARGAYSPESQIRARVWTFDENEPVDHAMFKR
RVSAALAHRKQWVRDTNAVRLIFGESDRLPGLIVDYYGQGERGQLVCQFNAAGVEQWKDALVQALIKETGCPNVYERSDA
AVRQREGLPLVTGVLAGAEPDPELSVTEHGVRYHVDVRNGHKTGFYVDQRDNRKLVGDLAEGREVLNCFCYTGGFSLAAL
RGGATSVTSIDSSGEALKIAASNVTLNGFDPARATWLDADVFKTLREFRAEGRQFDLIVLDPPKFAPSAQHIDRAARAYK
EINLVGMQLLRPGGLLFTYSCSGAISMELFQKIVAGAATDARSDARILRRLSAGTDHPMSAAFPEGEYLKGLLLEKI
>Mature_397_residues
MNVITLKPGKEKSLLRRHPWVYATGIAATEGRCEPGSTVVIRSADGRFLARGAYSPESQIRARVWTFDENEPVDHAMFKR
RVSAALAHRKQWVRDTNAVRLIFGESDRLPGLIVDYYGQGERGQLVCQFNAAGVEQWKDALVQALIKETGCPNVYERSDA
AVRQREGLPLVTGVLAGAEPDPELSVTEHGVRYHVDVRNGHKTGFYVDQRDNRKLVGDLAEGREVLNCFCYTGGFSLAAL
RGGATSVTSIDSSGEALKIAASNVTLNGFDPARATWLDADVFKTLREFRAEGRQFDLIVLDPPKFAPSAQHIDRAARAYK
EINLVGMQLLRPGGLLFTYSCSGAISMELFQKIVAGAATDARSDARILRRLSAGTDHPMSAAFPEGEYLKGLLLEKI

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=392, Percent_Identity=47.4489795918367, Blast_Score=370, Evalue=1e-104,
Organism=Escherichia coli, GI1787181, Length=168, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 43609; Mature: 43609

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVITLKPGKEKSLLRRHPWVYATGIAATEGRCEPGSTVVIRSADGRFLARGAYSPESQI
CCEEEECCCCCHHHHHHCCCEEEECEEECCCCCCCCCEEEEECCCCCEEEECCCCCHHHH
RARVWTFDENEPVDHAMFKRRVSAALAHRKQWVRDTNAVRLIFGESDRLPGLIVDYYGQG
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCC
ERGQLVCQFNAAGVEQWKDALVQALIKETGCPNVYERSDAAVRQREGLPLVTGVLAGAEP
CCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHCCCCEEEHHHCCCCC
DPELSVTEHGVRYHVDVRNGHKTGFYVDQRDNRKLVGDLAEGREVLNCFCYTGGFSLAAL
CCCCEEECCCEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHEEEEECCCCEEEEE
RGGATSVTSIDSSGEALKIAASNVTLNGFDPARATWLDADVFKTLREFRAEGRQFDLIVL
CCCCCEEEEECCCCCEEEEEEEEEEECCCCCCHHEEECHHHHHHHHHHHHCCCEEEEEEE
DPPKFAPSAQHIDRAARAYKEINLVGMQLLRPGGLLFTYSCSGAISMELFQKIVAGAATD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCC
ARSDARILRRLSAGTDHPMSAAFPEGEYLKGLLLEKI
CHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MNVITLKPGKEKSLLRRHPWVYATGIAATEGRCEPGSTVVIRSADGRFLARGAYSPESQI
CCEEEECCCCCHHHHHHCCCEEEECEEECCCCCCCCCEEEEECCCCCEEEECCCCCHHHH
RARVWTFDENEPVDHAMFKRRVSAALAHRKQWVRDTNAVRLIFGESDRLPGLIVDYYGQG
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCC
ERGQLVCQFNAAGVEQWKDALVQALIKETGCPNVYERSDAAVRQREGLPLVTGVLAGAEP
CCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHCCCCEEEHHHCCCCC
DPELSVTEHGVRYHVDVRNGHKTGFYVDQRDNRKLVGDLAEGREVLNCFCYTGGFSLAAL
CCCCEEECCCEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHEEEEECCCCEEEEE
RGGATSVTSIDSSGEALKIAASNVTLNGFDPARATWLDADVFKTLREFRAEGRQFDLIVL
CCCCCEEEEECCCCCEEEEEEEEEEECCCCCCHHEEECHHHHHHHHHHHHCCCEEEEEEE
DPPKFAPSAQHIDRAARAYKEINLVGMQLLRPGGLLFTYSCSGAISMELFQKIVAGAATD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCC
ARSDARILRRLSAGTDHPMSAAFPEGEYLKGLLLEKI
CHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA