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The map label for this gene is nppD

Identifier: 94309167

GI number: 94309167

Start: 235860

End: 236837

Strand: Reverse

Name: nppD

Synonym: Rmet_0222

Alternate gene names: NA

Gene position: 236837-235860 (Counterclockwise)

Preceding gene: 94309168

Following gene: 94309166

Centisome position: 6.03

GC content: 64.42

Gene sequence:

>978_bases
ATGCCTGCCGCCAAGCTTATTCCGCCAGTGCTGCAAAACCTGGCCCTGCCCGTTATCGCGTCGCCAATGTTCATCGTCAG
CTATCCGGAACTGGTGCTGGCGCAGTGCAAGGCCGGCATCGTCGGCTCGTTCCCCGCGCTAAACGCGCGCCCCGCCGAAC
TGCTCGATGAATGGCTGACCCAGATCCAAGCGGAACTGGCCAACTTCAAGGCGGCGAATCCCGGCAAGCCGGTGGGCCCG
ATCGCGGTCAACCAGATCGTGCACGCCTCCAACGCGCGCCTCGAGCATGACGTGAAGGTCTGCGTCGAACACAAGGTGCC
GATCTTCATCACGTCGCTGCGCGCGCCGCCCAAGGAAATGATCGACGCCGTGCACAGCTACGGCGGCATCGTGCTGCACG
ACGTCATCAACATTCGCCACGCTGAAAAAGCGATCGAGGCCGGCGTTGACGGCCTGATCCTGGTGGCGGCCGGTGCAGGC
GGCCATGCCGGCACGCTATCGCCGTTCGCGCTGGTGGGCGAAGTGCGCAAGTTCTTCGACGGCCCGATCGCGCTGTCCGG
CTCCATCGCCACCGGTGAAGCCGTGCTGGCCGCGCAGGCCATGGGCGCGGATTTCGCCTACGTGGGCACACGCTTCATCG
CATCGCAAGAGGCGCACGCAGCGCTCGAGTACAAGCAGTCGATCACCAGTTCGGCCGCGTCGGACATCATCTACACGAAC
CTGTTCACGGGTGTGCACGGCAACTACATCCGCGAGAGCATCGTCAATTCGGGGCTCGATCCGGAGAACCTGCCCGTGGC
CGACAAGAGCAAGATGGACTTCTCCAGCGGCAGTTCGAAGGCCAAGGCATGGAAGGACATCTGGGGCGCCGGTCAGGGCG
TGGGCCAGATCGAAGACATCCCCACCGCCGGTGAGATCGTGGCACGCATGAAGAGCGAGTACGACGCCGCGCGTGCCCGC
CTGGGGCTGGTAGCATAA

Upstream 100 bases:

>100_bases
AAGGCAAGCGCATAGACTGCGCCCTCTAGCCCGGCGCCACGCTACCCGAAACTCCCTATAATCGAACGATCGTTCCATAA
CACGGATGTGAGGAGACTTC

Downstream 100 bases:

>100_bases
GGGCGTTTCCGCCCTTGGCGCGCTAGCCGCATTTCTCCAGTTGCAACCAGACGACCCCGAACGCCAGACCACGCTAACGC
ACCGCGAACGCGCCATTCGA

Product: 2-nitropropane dioxygenase

Products: NA

Alternate protein names: Nitroalkane oxidase [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MPAAKLIPPVLQNLALPVIASPMFIVSYPELVLAQCKAGIVGSFPALNARPAELLDEWLTQIQAELANFKAANPGKPVGP
IAVNQIVHASNARLEHDVKVCVEHKVPIFITSLRAPPKEMIDAVHSYGGIVLHDVINIRHAEKAIEAGVDGLILVAAGAG
GHAGTLSPFALVGEVRKFFDGPIALSGSIATGEAVLAAQAMGADFAYVGTRFIASQEAHAALEYKQSITSSAASDIIYTN
LFTGVHGNYIRESIVNSGLDPENLPVADKSKMDFSSGSSKAKAWKDIWGAGQGVGQIEDIPTAGEIVARMKSEYDAARAR
LGLVA

Sequences:

>Translated_325_residues
MPAAKLIPPVLQNLALPVIASPMFIVSYPELVLAQCKAGIVGSFPALNARPAELLDEWLTQIQAELANFKAANPGKPVGP
IAVNQIVHASNARLEHDVKVCVEHKVPIFITSLRAPPKEMIDAVHSYGGIVLHDVINIRHAEKAIEAGVDGLILVAAGAG
GHAGTLSPFALVGEVRKFFDGPIALSGSIATGEAVLAAQAMGADFAYVGTRFIASQEAHAALEYKQSITSSAASDIIYTN
LFTGVHGNYIRESIVNSGLDPENLPVADKSKMDFSSGSSKAKAWKDIWGAGQGVGQIEDIPTAGEIVARMKSEYDAARAR
LGLVA
>Mature_324_residues
PAAKLIPPVLQNLALPVIASPMFIVSYPELVLAQCKAGIVGSFPALNARPAELLDEWLTQIQAELANFKAANPGKPVGPI
AVNQIVHASNARLEHDVKVCVEHKVPIFITSLRAPPKEMIDAVHSYGGIVLHDVINIRHAEKAIEAGVDGLILVAAGAGG
HAGTLSPFALVGEVRKFFDGPIALSGSIATGEAVLAAQAMGADFAYVGTRFIASQEAHAALEYKQSITSSAASDIIYTNL
FTGVHGNYIRESIVNSGLDPENLPVADKSKMDFSSGSSKAKAWKDIWGAGQGVGQIEDIPTAGEIVARMKSEYDAARARL
GLVA

Specific function: Catalyzes the oxidation of alkyl nitronates to produce the corresponding carbonyl compounds and nitrites [H]

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitronate monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004136
- InterPro:   IPR013785 [H]

Pfam domain/function: PF03060 NPD [H]

EC number: =1.13.12.16 [H]

Molecular weight: Translated: 34147; Mature: 34015

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAAKLIPPVLQNLALPVIASPMFIVSYPELVLAQCKAGIVGSFPALNARPAELLDEWLT
CCCHHHHHHHHHHHHHHHHHCCHHEECCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
QIQAELANFKAANPGKPVGPIAVNQIVHASNARLEHDVKVCVEHKVPIFITSLRAPPKEM
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCHHHH
IDAVHSYGGIVLHDVINIRHAEKAIEAGVDGLILVAAGAGGHAGTLSPFALVGEVRKFFD
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHC
GPIALSGSIATGEAVLAAQAMGADFAYVGTRFIASQEAHAALEYKQSITSSAASDIIYTN
CCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFTGVHGNYIRESIVNSGLDPENLPVADKSKMDFSSGSSKAKAWKDIWGAGQGVGQIEDI
HHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCC
PTAGEIVARMKSEYDAARARLGLVA
CCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PAAKLIPPVLQNLALPVIASPMFIVSYPELVLAQCKAGIVGSFPALNARPAELLDEWLT
CCHHHHHHHHHHHHHHHHHCCHHEECCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
QIQAELANFKAANPGKPVGPIAVNQIVHASNARLEHDVKVCVEHKVPIFITSLRAPPKEM
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCHHHH
IDAVHSYGGIVLHDVINIRHAEKAIEAGVDGLILVAAGAGGHAGTLSPFALVGEVRKFFD
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHC
GPIALSGSIATGEAVLAAQAMGADFAYVGTRFIASQEAHAALEYKQSITSSAASDIIYTN
CCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFTGVHGNYIRESIVNSGLDPENLPVADKSKMDFSSGSSKAKAWKDIWGAGQGVGQIEDI
HHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCC
PTAGEIVARMKSEYDAARARLGLVA
CCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA