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The map label for this gene is dmlR [H]

Identifier: 94309144

GI number: 94309144

Start: 207472

End: 208398

Strand: Reverse

Name: dmlR [H]

Synonym: Rmet_0199

Alternate gene names: 94309144

Gene position: 208398-207472 (Counterclockwise)

Preceding gene: 94309149

Following gene: 94309143

Centisome position: 5.31

GC content: 68.5

Gene sequence:

>927_bases
ATGGATGCCTTCTCCGATCTCGCGTTCTTCGTGTTGCTCGTCAAGCGCGGCAGTCTTGCCGGCGCGGCGCAGCAGCTTGG
CGTCACGCCATCGGCGACCAGCAAGCGGCTGGCGGCGCTTGAGGCACGCCTGGGGGTGCGGCTGCTGAATCGCACCACGC
GGCGACTGTCGGTCACGCCGGAGGGCGAGGCATATCTGGCCCAGGGTGGAAGAATCCTGGCCGAGCTCGACGAGCTGGAG
CAGGGCTTGCGTGGCAGCCGCGTGGCGCCGCGCGGCCTGCTGCGCGTCAACGGCACGCTCGGGTTCGGACGCCGCCACCT
GGCACCCGCCATCGGTGATTTTCTCGTGGCCTGTCCCGATATCGAAGTGCAGCTTCAGTTGACTGACCGCGCGCTGAATC
TGGCCGAGGAAGGCTACGACGTCGGCATCCTGGTGGGTGACCTGCCCGACGCCCGCCTCAACGCCCGACGGCTCGCGCTC
AATAGCCGGCTCCTGTGCGCATCCCCCGCCTACCTCGCCCGCCACGGCGAACCCGCCTCGCCACGCGCGCTGACGCAGCA
TCGCTGCATCGTAATCCGCGAGAGCGAGGCCGCTTACGGTACCTGGCACCTCCAGCACCAGCAATCCGCCGCACGGCAGG
AGACGGTCAAGGTACGCGGCATGGCGGCGACCAACGATGGCGAGGTGGCCGTCAACTGGGCGCTGCAGGGCCACGGCATC
GTGCTGAGGTCCGAATGGGAGGTCGCGCCGCTGCTGCGCTCGGGACGGCTGCGGCAAGTGTTGCCGGAGTGGTCCGCGCA
GGCCGCCGACATCCATGCGGTGTATCTGTCTCGTGACAAGCTCTCGGCACGCGTACGTGCTTTCGTCGACTTCCTCGCCG
AGCGCTTCGGTCACTACGACCCCGCCGCGCCATATCACGGGTGGTAG

Upstream 100 bases:

>100_bases
AATGCACGCAGTCTAATCGCGGAATCCATCGCTATAATTTCGCCAAATTACTTACATTCGTGCCTTTAATTCACGAATGC
ATCACGAATAATCGGCCGCC

Downstream 100 bases:

>100_bases
CTCCCCGTTGGTAGCCGACTGCAGCCGGGCAGCCATGTCGACGGCAGGGATGAAGGCCCTGTCGACCGCCCATGCTGCTA
CCCTTCGCGCATACTTCAAA

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MDAFSDLAFFVLLVKRGSLAGAAQQLGVTPSATSKRLAALEARLGVRLLNRTTRRLSVTPEGEAYLAQGGRILAELDELE
QGLRGSRVAPRGLLRVNGTLGFGRRHLAPAIGDFLVACPDIEVQLQLTDRALNLAEEGYDVGILVGDLPDARLNARRLAL
NSRLLCASPAYLARHGEPASPRALTQHRCIVIRESEAAYGTWHLQHQQSAARQETVKVRGMAATNDGEVAVNWALQGHGI
VLRSEWEVAPLLRSGRLRQVLPEWSAQAADIHAVYLSRDKLSARVRAFVDFLAERFGHYDPAAPYHGW

Sequences:

>Translated_308_residues
MDAFSDLAFFVLLVKRGSLAGAAQQLGVTPSATSKRLAALEARLGVRLLNRTTRRLSVTPEGEAYLAQGGRILAELDELE
QGLRGSRVAPRGLLRVNGTLGFGRRHLAPAIGDFLVACPDIEVQLQLTDRALNLAEEGYDVGILVGDLPDARLNARRLAL
NSRLLCASPAYLARHGEPASPRALTQHRCIVIRESEAAYGTWHLQHQQSAARQETVKVRGMAATNDGEVAVNWALQGHGI
VLRSEWEVAPLLRSGRLRQVLPEWSAQAADIHAVYLSRDKLSARVRAFVDFLAERFGHYDPAAPYHGW
>Mature_308_residues
MDAFSDLAFFVLLVKRGSLAGAAQQLGVTPSATSKRLAALEARLGVRLLNRTTRRLSVTPEGEAYLAQGGRILAELDELE
QGLRGSRVAPRGLLRVNGTLGFGRRHLAPAIGDFLVACPDIEVQLQLTDRALNLAEEGYDVGILVGDLPDARLNARRLAL
NSRLLCASPAYLARHGEPASPRALTQHRCIVIRESEAAYGTWHLQHQQSAARQETVKVRGMAATNDGEVAVNWALQGHGI
VLRSEWEVAPLLRSGRLRQVLPEWSAQAADIHAVYLSRDKLSARVRAFVDFLAERFGHYDPAAPYHGW

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=298, Percent_Identity=40.9395973154362, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1789440, Length=301, Percent_Identity=38.5382059800665, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1786401, Length=290, Percent_Identity=35.8620689655172, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI145693193, Length=300, Percent_Identity=32.6666666666667, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=28.0276816608997, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1789639, Length=296, Percent_Identity=28.7162162162162, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787589, Length=293, Percent_Identity=26.6211604095563, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788706, Length=299, Percent_Identity=25.0836120401338, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1786448, Length=243, Percent_Identity=26.3374485596708, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790011, Length=306, Percent_Identity=24.1830065359477, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1789173, Length=243, Percent_Identity=26.3374485596708, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI157672245, Length=180, Percent_Identity=30, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33714; Mature: 33714

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAFSDLAFFVLLVKRGSLAGAAQQLGVTPSATSKRLAALEARLGVRLLNRTTRRLSVTP
CCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCC
EGEAYLAQGGRILAELDELEQGLRGSRVAPRGLLRVNGTLGFGRRHLAPAIGDFLVACPD
CCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHEEECCC
IEVQLQLTDRALNLAEEGYDVGILVGDLPDARLNARRLALNSRLLCASPAYLARHGEPAS
CEEEEEEEHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCEEECCCHHHHHCCCCCC
PRALTQHRCIVIRESEAAYGTWHLQHQQSAARQETVKVRGMAATNDGEVAVNWALQGHGI
CCHHCCCEEEEEECCCCCCEEEEEHHHHHHHHHHHEEEEEEEECCCCCEEEEEEEECCCE
VLRSEWEVAPLLRSGRLRQVLPEWSAQAADIHAVYLSRDKLSARVRAFVDFLAERFGHYD
EEECCCCHHHHHHCCCHHHHHHHCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHCCCC
PAAPYHGW
CCCCCCCC
>Mature Secondary Structure
MDAFSDLAFFVLLVKRGSLAGAAQQLGVTPSATSKRLAALEARLGVRLLNRTTRRLSVTP
CCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCC
EGEAYLAQGGRILAELDELEQGLRGSRVAPRGLLRVNGTLGFGRRHLAPAIGDFLVACPD
CCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHEEECCC
IEVQLQLTDRALNLAEEGYDVGILVGDLPDARLNARRLALNSRLLCASPAYLARHGEPAS
CEEEEEEEHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCEEECCCHHHHHCCCCCC
PRALTQHRCIVIRESEAAYGTWHLQHQQSAARQETVKVRGMAATNDGEVAVNWALQGHGI
CCHHCCCEEEEEECCCCCCEEEEEHHHHHHHHHHHEEEEEEEECCCCCEEEEEEEECCCE
VLRSEWEVAPLLRSGRLRQVLPEWSAQAADIHAVYLSRDKLSARVRAFVDFLAERFGHYD
EEECCCCHHHHHHCCCHHHHHHHCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHCCCC
PAAPYHGW
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]