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The map label for this gene is dmeF [H]

Identifier: 94309143

GI number: 94309143

Start: 206274

End: 207422

Strand: Reverse

Name: dmeF [H]

Synonym: Rmet_0198

Alternate gene names: 94309143

Gene position: 207422-206274 (Counterclockwise)

Preceding gene: 94309144

Following gene: 94309130

Centisome position: 5.28

GC content: 64.58

Gene sequence:

>1149_bases
ATGAAGGCCCTGTCGACCGCCCATGCTGCTACCCTTCGCGCATACTTCAAACCCGATCCAACCCATCCGGACTGCACCGC
CATGCACACGCAAAACCTCTCCGCGTGGACCCACTCCCACGTGTTCGACGCAGGCAACCAGGCGGCCGAACGCGGCACGC
GGCTAGTCATGTGGATCACGCTGGCGATGATGATCGTCGAAATCGCCGCGGGTCTCGTCTTCAACTCGATGGCACTGCTA
GCCGATGGCTGGCACATGAGCTCGCACGCACTGGCCATCGGTCTGTCCGCGTTCGCCTATGCGGCGGCCCGCCGGCTGTC
GCAGGATGGCCGATTCTCTTTCGGAACCTGGAAGATCGAAGTTCTCGCCGCATTCGCCAGCGCCATCTTCCTGCTTGGCG
TCGCTGGCCTGATGGTGTTTGGCTCGGTCGAGCGGCTGTTCACCCCCCAGCCGATTCACTATCAGGAGGCGATGGCGATC
ACCGCGATCGGCCTGATCGTCAACCTGGCCTGTGCGTTGATCCTCGGCGGCGCGCACCACGGCCATGATCACGGGCACGG
GCACGACCACGGCCATCAGGCGCACGGGCATGATCACCACGATCATGGTCATGGTCATGGTCATGGTCACGGCGGTCACG
ACGATCATGGCCACCGCCATCACGACATCAACCTGCGTTCGGCCTATCTGCACGTGGTGGCCGATGCCGCCACCTCGGTA
TTGGCGATCGTTGCATTGGCGGGTGGCTGGTGGCTGGGATGGAGCTGGCTCGATCCGGTGATGGGTCTGGTCGGCGCGGT
GTTGGTAGGCCGCTGGGCCATCGGCCTGATGCGCCAGAGCGGGACGGTGCTGCTCGATCGCGAGATGGATCACCCGGTGG
TCGAAGAAGTACGCGAAGTGCTGGCGCAGTTCTCGCACGGCGAGGACGGCACGCGCGTGGCCGACCTGCACGTCTGGCGC
GTGGGCCGTGAAAAATTCGCGTGCATCGCCAGCCTGGTCACCCACGACGCCAGCCTGACGCCGCAGCGCGTGCGGCACGC
GCTATCGATACACGATGAACTCGTGCACGTGAGCGTCGAAATCAATCAATGCGACGATGGCCGAGACGGCCACGATGGCC
ACGCGGCGCATCGCGGCCACCGTCACTAG

Upstream 100 bases:

>100_bases
GCCGAGCGCTTCGGTCACTACGACCCCGCCGCGCCATATCACGGGTGGTAGCTCCCCGTTGGTAGCCGACTGCAGCCGGG
CAGCCATGTCGACGGCAGGG

Downstream 100 bases:

>100_bases
GCGGCAAACCCGCTCAGGTCACTCAGGGCAGCAGCAGGATCGCGCCGGTAGTCGCCCGGCCTTCGGCAGCCTCGTGCGCG
CGCGCCACGTCCGCCAGCGG

Product: Co/Zn/Cd efflux system component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKALSTAHAATLRAYFKPDPTHPDCTAMHTQNLSAWTHSHVFDAGNQAAERGTRLVMWITLAMMIVEIAAGLVFNSMALL
ADGWHMSSHALAIGLSAFAYAAARRLSQDGRFSFGTWKIEVLAAFASAIFLLGVAGLMVFGSVERLFTPQPIHYQEAMAI
TAIGLIVNLACALILGGAHHGHDHGHGHDHGHQAHGHDHHDHGHGHGHGHGGHDDHGHRHHDINLRSAYLHVVADAATSV
LAIVALAGGWWLGWSWLDPVMGLVGAVLVGRWAIGLMRQSGTVLLDREMDHPVVEEVREVLAQFSHGEDGTRVADLHVWR
VGREKFACIASLVTHDASLTPQRVRHALSIHDELVHVSVEINQCDDGRDGHDGHAAHRGHRH

Sequences:

>Translated_382_residues
MKALSTAHAATLRAYFKPDPTHPDCTAMHTQNLSAWTHSHVFDAGNQAAERGTRLVMWITLAMMIVEIAAGLVFNSMALL
ADGWHMSSHALAIGLSAFAYAAARRLSQDGRFSFGTWKIEVLAAFASAIFLLGVAGLMVFGSVERLFTPQPIHYQEAMAI
TAIGLIVNLACALILGGAHHGHDHGHGHDHGHQAHGHDHHDHGHGHGHGHGGHDDHGHRHHDINLRSAYLHVVADAATSV
LAIVALAGGWWLGWSWLDPVMGLVGAVLVGRWAIGLMRQSGTVLLDREMDHPVVEEVREVLAQFSHGEDGTRVADLHVWR
VGREKFACIASLVTHDASLTPQRVRHALSIHDELVHVSVEINQCDDGRDGHDGHAAHRGHRH
>Mature_382_residues
MKALSTAHAATLRAYFKPDPTHPDCTAMHTQNLSAWTHSHVFDAGNQAAERGTRLVMWITLAMMIVEIAAGLVFNSMALL
ADGWHMSSHALAIGLSAFAYAAARRLSQDGRFSFGTWKIEVLAAFASAIFLLGVAGLMVFGSVERLFTPQPIHYQEAMAI
TAIGLIVNLACALILGGAHHGHDHGHGHDHGHQAHGHDHHDHGHGHGHGHGGHDDHGHRHHDINLRSAYLHVVADAATSV
LAIVALAGGWWLGWSWLDPVMGLVGAVLVGRWAIGLMRQSGTVLLDREMDHPVVEEVREVLAQFSHGEDGTRVADLHVWR
VGREKFACIASLVTHDASLTPQRVRHALSIHDELVHVSVEINQCDDGRDGHDGHAAHRGHRH

Specific function: Involved in divalent cation and potassium homeostasis in the cell. Catalyzes the active efflux of zinc, cadmium and cobalt, in exchange for potassium and H(+) ions [H]

COG id: COG1230

COG function: function code P; Co/Zn/Cd efflux system component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily [H]

Homologues:

Organism=Homo sapiens, GI222080086, Length=332, Percent_Identity=25.6024096385542, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI40255009, Length=332, Percent_Identity=25.6024096385542, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI20070323, Length=297, Percent_Identity=28.2828282828283, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI21359890, Length=275, Percent_Identity=25.4545454545455, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1786966, Length=317, Percent_Identity=27.7602523659306, Blast_Score=85, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17555794, Length=347, Percent_Identity=26.8011527377522, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17555796, Length=344, Percent_Identity=26.453488372093, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI32564488, Length=273, Percent_Identity=27.4725274725275, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI193210990, Length=283, Percent_Identity=27.9151943462898, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323899, Length=341, Percent_Identity=24.3401759530792, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24656728, Length=308, Percent_Identity=26.6233766233766, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24656724, Length=308, Percent_Identity=26.6233766233766, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24656720, Length=308, Percent_Identity=26.6233766233766, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24656715, Length=308, Percent_Identity=26.6233766233766, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21357035, Length=308, Percent_Identity=26.6233766233766, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24645863, Length=242, Percent_Identity=26.4462809917355, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583858, Length=309, Percent_Identity=26.8608414239482, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002524 [H]

Pfam domain/function: PF01545 Cation_efflux [H]

EC number: NA

Molecular weight: Translated: 41510; Mature: 41510

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALSTAHAATLRAYFKPDPTHPDCTAMHTQNLSAWTHSHVFDAGNQAAERGTRLVMWIT
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHCCCCHHHHHCCEEHHHHH
LAMMIVEIAAGLVFNSMALLADGWHMSSHALAIGLSAFAYAAARRLSQDGRFSFGTWKIE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHH
VLAAFASAIFLLGVAGLMVFGSVERLFTPQPIHYQEAMAITAIGLIVNLACALILGGAHH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
GHDHGHGHDHGHQAHGHDHHDHGHGHGHGHGGHDDHGHRHHDINLRSAYLHVVADAATSV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LAIVALAGGWWLGWSWLDPVMGLVGAVLVGRWAIGLMRQSGTVLLDREMDHPVVEEVREV
HHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHH
LAQFSHGEDGTRVADLHVWRVGREKFACIASLVTHDASLTPQRVRHALSIHDELVHVSVE
HHHHCCCCCCCEEEEEEEEHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEEEEE
INQCDDGRDGHDGHAAHRGHRH
EEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKALSTAHAATLRAYFKPDPTHPDCTAMHTQNLSAWTHSHVFDAGNQAAERGTRLVMWIT
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHCCCCHHHHHCCEEHHHHH
LAMMIVEIAAGLVFNSMALLADGWHMSSHALAIGLSAFAYAAARRLSQDGRFSFGTWKIE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHH
VLAAFASAIFLLGVAGLMVFGSVERLFTPQPIHYQEAMAITAIGLIVNLACALILGGAHH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
GHDHGHGHDHGHQAHGHDHHDHGHGHGHGHGGHDDHGHRHHDINLRSAYLHVVADAATSV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LAIVALAGGWWLGWSWLDPVMGLVGAVLVGRWAIGLMRQSGTVLLDREMDHPVVEEVREV
HHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHH
LAQFSHGEDGTRVADLHVWRVGREKFACIASLVTHDASLTPQRVRHALSIHDELVHVSVE
HHHHCCCCCCCEEEEEEEEHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEEEEE
INQCDDGRDGHDGHAAHRGHRH
EEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377; 9099864 [H]