Click here to switch to the map view.

The map label for this gene is menB [C]

Identifier: 94309058

GI number: 94309058

Start: 119221

End: 120009

Strand: Direct

Name: menB [C]

Synonym: Rmet_0113

Alternate gene names: 94309058

Gene position: 119221-120009 (Clockwise)

Preceding gene: 94309057

Following gene: 94309059

Centisome position: 3.04

GC content: 67.55

Gene sequence:

>789_bases
ATGCAATTCACCACGCTTACGGTTACCGCTGAACGCCACGTTGCGACGGTCACGCTCAATCGTCCCGACGTCCGCAACGC
ATTCAACGAGACGGTCATCGCGGAACTGACCGGTGCGTTCCGTGCGCTTGGCGATACGCCCGACGTGCGCGCGATCGTGC
TGGCGGGCAACGGCCCGGCCTTCTGCGCCGGCGCGGATCTGAACTGGATGAAGAAGATGGCCGGTTACTCGAATGACGAG
AACCGGGCCGACGCGCTGACGCTGGCGCAGATGCTGCACACGATCTGGTCCTGCCCGAAGCCCGTGATCGCGCGTGTGCA
CGGCGATACTTATGCCGGCGGCGTGGGGCTGGTGGCGGCATGCGATGTGGCGGTAGCGGCCGAGGGCGTGAACTTCTGCC
TGTCCGAAGCGCGCCTGGGCCTGTTGCCGGCGACGATCAGCCCGTATGTGATTCGCGCGATGGGCGAGCAGGCCGCGCGC
CGCTACTTCATCACCGCAGAGCGGTTCGATGCGGCGGAAGCGCTGCGCATCGGCTTCGTCCATGCCACGGTGCCGGCGGC
GGGGCTCGACGCCAAGGTGGCCGAACTGGCCGCGACGCTGGTCGGCAACAGCCCGAATGCCGTGCGCGAAAGCAAGCGGC
TGGTGCAGGACGTGGCCGGCCGCGCCATCGACAAGGACCTGCTGACCGATACCGCGGACCGGATCGCCGTCATCCGCGCG
TCAGAGGAGGGCCGCGAAGGCGTGCGTTCGTTCCTGGAAAAGCGCGCGCCTGCCTGGAAGCCGCAGTAA

Upstream 100 bases:

>100_bases
CACGCGCGGCGTGACCAGCAACCGGCGCCAGAAATCCTGAATTGGCCGCATTCGACGGAATTCGGCCCCGTCCATTACAC
ATCCGTTTCTCGGAGGAGAC

Downstream 100 bases:

>100_bases
CGGCAATCGGGCGCCAGCCCTCTCGCGCCCGCGGGAGGGTGCGACCCCGCCACATCGAGAGATTTTTTTTGTAGTGCGTT
AGCCGATGTTATCGGCGCGT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MQFTTLTVTAERHVATVTLNRPDVRNAFNETVIAELTGAFRALGDTPDVRAIVLAGNGPAFCAGADLNWMKKMAGYSNDE
NRADALTLAQMLHTIWSCPKPVIARVHGDTYAGGVGLVAACDVAVAAEGVNFCLSEARLGLLPATISPYVIRAMGEQAAR
RYFITAERFDAAEALRIGFVHATVPAAGLDAKVAELAATLVGNSPNAVRESKRLVQDVAGRAIDKDLLTDTADRIAVIRA
SEEGREGVRSFLEKRAPAWKPQ

Sequences:

>Translated_262_residues
MQFTTLTVTAERHVATVTLNRPDVRNAFNETVIAELTGAFRALGDTPDVRAIVLAGNGPAFCAGADLNWMKKMAGYSNDE
NRADALTLAQMLHTIWSCPKPVIARVHGDTYAGGVGLVAACDVAVAAEGVNFCLSEARLGLLPATISPYVIRAMGEQAAR
RYFITAERFDAAEALRIGFVHATVPAAGLDAKVAELAATLVGNSPNAVRESKRLVQDVAGRAIDKDLLTDTADRIAVIRA
SEEGREGVRSFLEKRAPAWKPQ
>Mature_262_residues
MQFTTLTVTAERHVATVTLNRPDVRNAFNETVIAELTGAFRALGDTPDVRAIVLAGNGPAFCAGADLNWMKKMAGYSNDE
NRADALTLAQMLHTIWSCPKPVIARVHGDTYAGGVGLVAACDVAVAAEGVNFCLSEARLGLLPATISPYVIRAMGEQAAR
RYFITAERFDAAEALRIGFVHATVPAAGLDAKVAELAATLVGNSPNAVRESKRLVQDVAGRAIDKDLLTDTADRIAVIRA
SEEGREGVRSFLEKRAPAWKPQ

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=258, Percent_Identity=29.8449612403101, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI31542718, Length=253, Percent_Identity=30.0395256916996, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI70995211, Length=197, Percent_Identity=37.0558375634518, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI260274832, Length=259, Percent_Identity=27.4131274131274, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI45643119, Length=259, Percent_Identity=27.4131274131274, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI260275230, Length=259, Percent_Identity=27.4131274131274, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI4502327, Length=262, Percent_Identity=29.0076335877863, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI37594471, Length=229, Percent_Identity=27.9475982532751, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI37594469, Length=227, Percent_Identity=28.1938325991189, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788597, Length=247, Percent_Identity=31.5789473684211, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787659, Length=267, Percent_Identity=32.2097378277154, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI221142681, Length=269, Percent_Identity=29.368029739777, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1787660, Length=252, Percent_Identity=27.7777777777778, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI87082183, Length=272, Percent_Identity=25.7352941176471, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1788682, Length=219, Percent_Identity=29.2237442922374, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=257, Percent_Identity=31.5175097276265, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=260, Percent_Identity=30, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=228, Percent_Identity=29.3859649122807, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17540306, Length=171, Percent_Identity=32.7485380116959, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17540714, Length=201, Percent_Identity=28.8557213930348, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=216, Percent_Identity=26.3888888888889, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=261, Percent_Identity=25.6704980842912, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=221, Percent_Identity=27.1493212669683, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24653139, Length=264, Percent_Identity=29.5454545454545, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19920382, Length=232, Percent_Identity=30.6034482758621, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19922422, Length=264, Percent_Identity=26.5151515151515, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI20129971, Length=245, Percent_Identity=28.1632653061224, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24653477, Length=245, Percent_Identity=28.1632653061224, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24654903, Length=222, Percent_Identity=26.1261261261261, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27973; Mature: 27973

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFTTLTVTAERHVATVTLNRPDVRNAFNETVIAELTGAFRALGDTPDVRAIVLAGNGPA
CCEEEEEEEECCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
FCAGADLNWMKKMAGYSNDENRADALTLAQMLHTIWSCPKPVIARVHGDTYAGGVGLVAA
EEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEECCCCCCCCHHHHHH
CDVAVAAEGVNFCLSEARLGLLPATISPYVIRAMGEQAARRYFITAERFDAAEALRIGFV
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEEHHHHCHHHHHHHEEE
HATVPAAGLDAKVAELAATLVGNSPNAVRESKRLVQDVAGRAIDKDLLTDTADRIAVIRA
EEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEC
SEEGREGVRSFLEKRAPAWKPQ
CCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQFTTLTVTAERHVATVTLNRPDVRNAFNETVIAELTGAFRALGDTPDVRAIVLAGNGPA
CCEEEEEEEECCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
FCAGADLNWMKKMAGYSNDENRADALTLAQMLHTIWSCPKPVIARVHGDTYAGGVGLVAA
EEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEECCCCCCCCHHHHHH
CDVAVAAEGVNFCLSEARLGLLPATISPYVIRAMGEQAARRYFITAERFDAAEALRIGFV
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEEEEHHHHCHHHHHHHEEE
HATVPAAGLDAKVAELAATLVGNSPNAVRESKRLVQDVAGRAIDKDLLTDTADRIAVIRA
EEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEC
SEEGREGVRSFLEKRAPAWKPQ
CCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA