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The map label for this gene is gatB [H]

Identifier: 94308991

GI number: 94308991

Start: 52552

End: 54006

Strand: Reverse

Name: gatB [H]

Synonym: Rmet_0046

Alternate gene names: 94308991

Gene position: 54006-52552 (Counterclockwise)

Preceding gene: 94308992

Following gene: 94308980

Centisome position: 1.37

GC content: 64.88

Gene sequence:

>1455_bases
ATGCAATGGGAAGTGGTGATCGGCCTGGAGACGCACACGCAGCTCTCCACGGCCTCCAAGATTTTCTCCGGCACCTCCAC
CGCGTTCGGAGCCGAGCCCAATACGCAGGCCTCGCCCGTGGACCTGGCGCTGCCGGGCGTGCTGCCCGTACTGAACCGCG
GCGCCGTGGAGCGCGCCATCCAGTTCGGCCTGTCCATTGGCGCGACGATTGCGCCACGCAGCATCTTCGCGCGCAAGAAC
TACTTCTATCCTGACCTGCCGAAGGGCTACCAGATCAGCCAGTACGAGATCCCCGTCGTGCAAGGCGGCCAGATCACGAT
TCAGGTGGAAGGCAAGCAGGGCGTGTACGAGAAGACCGTCAACCTGACGCGCGCCCACCTTGAGGAGGACGCCGGCAAGT
CGCTGCACGAGGACTTCGCGGGCATGACCGGCATCGACCTGAACCGCGCGGGCACGCCGCTGCTAGAAATCGTGACCGAG
CCCGACATGCGCAGCTCGGCCGAGGCCGTGGCCTATGCCAAGGCCCTGCATTCGCTGGTGGTGTGGCTTGGCATCTGCGA
CGGCAACATGCAGGAAGGCAGCTTCCGCTGCGACGCCAATGTGTCCGTGCGTCCGGTGGGCCAGGAAAAATTCGGCACAC
GCCGCGAGATCAAGAACCTGAACTCGTTCCGCTTCCTGCAACAGGCCATCGACTACGAGGTGCAGTGGCAGATCGCCGAG
ATCGAGGATGGCCGCGAGATCGTGCAGGCCACCGTGCTGTTCGACCCGGACACCGGCGAGACACGCGCGATGCGCACCAA
GGAAGACGCGCACGACTACCGCTACTTCCCCGATCCGGACCTGATGCCGCTGGAGATCGACGCGGCGTGGGTCGAGCGCG
TGAAGAGCGAACTGCCCGAATTGCCGGCCACGATGCAGGCCCGTTTCGTGTCCCAGTACGGCCTGTCGGCGTACGACGCA
AGCACCATCACGGCGACGAAGGCGCTGGCGGCGTACTACGAGGCCGTCGTGACCAACGTTGGCGCAGCCAGCGCCAAGCC
GGCGGCGAACTGGGTGATGGGCGAGATCGCGTCGCAACTGAACCGCGAAGGCATCTCGATCGACGAAGCGCCCGTGCGCC
CGGCGCAACTGGCTAAGCTGCTGGCACGCATTACGGACGGCACGGTGTCGAACAACACCGCCAAGAAGGACGTGTTCCCG
GCGATGTGGGCTGGCGAGTCCGACGGCGACGCCGACGCGATCATCGCGGCCAAGGGCCTGAAGCAGATGTCCGACACTGG
CGAGCTGGAGAAGATCATCGACGACGTGCTGGCCGCCAACGCCAAGTCAGTCGAGGAATTCCGCGCTGGCAAGGAGAAGG
CGTTCAACGCACTGGTCGGCCAGGCCATGAAGGCCACCAAGGGCAAGGCTAACCCGGCGCAGGTCAATGAACTGCTGAAG
AAGAAGCTGGGCTGA

Upstream 100 bases:

>100_bases
CTTGCGAGCCCGGATCCCTGATACAACCGGGACCCGGCTCGACGAGCACCGAACACAGATACAGAAAGACTCAAATCAGG
CTTACGAAGGAACAACCGCC

Downstream 100 bases:

>100_bases
TATCGGCGCTCCGGAAGTGAAGAAGCCGGCCATGCATCAAGCGTGGCCGGTTTTTTTGCGCCTGGCGTTCGCTATGCCGA
CATCACCGGCACCACGCTGA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MQWEVVIGLETHTQLSTASKIFSGTSTAFGAEPNTQASPVDLALPGVLPVLNRGAVERAIQFGLSIGATIAPRSIFARKN
YFYPDLPKGYQISQYEIPVVQGGQITIQVEGKQGVYEKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTE
PDMRSSAEAVAYAKALHSLVVWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRREIKNLNSFRFLQQAIDYEVQWQIAE
IEDGREIVQATVLFDPDTGETRAMRTKEDAHDYRYFPDPDLMPLEIDAAWVERVKSELPELPATMQARFVSQYGLSAYDA
STITATKALAAYYEAVVTNVGAASAKPAANWVMGEIASQLNREGISIDEAPVRPAQLAKLLARITDGTVSNNTAKKDVFP
AMWAGESDGDADAIIAAKGLKQMSDTGELEKIIDDVLAANAKSVEEFRAGKEKAFNALVGQAMKATKGKANPAQVNELLK
KKLG

Sequences:

>Translated_484_residues
MQWEVVIGLETHTQLSTASKIFSGTSTAFGAEPNTQASPVDLALPGVLPVLNRGAVERAIQFGLSIGATIAPRSIFARKN
YFYPDLPKGYQISQYEIPVVQGGQITIQVEGKQGVYEKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTE
PDMRSSAEAVAYAKALHSLVVWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRREIKNLNSFRFLQQAIDYEVQWQIAE
IEDGREIVQATVLFDPDTGETRAMRTKEDAHDYRYFPDPDLMPLEIDAAWVERVKSELPELPATMQARFVSQYGLSAYDA
STITATKALAAYYEAVVTNVGAASAKPAANWVMGEIASQLNREGISIDEAPVRPAQLAKLLARITDGTVSNNTAKKDVFP
AMWAGESDGDADAIIAAKGLKQMSDTGELEKIIDDVLAANAKSVEEFRAGKEKAFNALVGQAMKATKGKANPAQVNELLK
KKLG
>Mature_484_residues
MQWEVVIGLETHTQLSTASKIFSGTSTAFGAEPNTQASPVDLALPGVLPVLNRGAVERAIQFGLSIGATIAPRSIFARKN
YFYPDLPKGYQISQYEIPVVQGGQITIQVEGKQGVYEKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTE
PDMRSSAEAVAYAKALHSLVVWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRREIKNLNSFRFLQQAIDYEVQWQIAE
IEDGREIVQATVLFDPDTGETRAMRTKEDAHDYRYFPDPDLMPLEIDAAWVERVKSELPELPATMQARFVSQYGLSAYDA
STITATKALAAYYEAVVTNVGAASAKPAANWVMGEIASQLNREGISIDEAPVRPAQLAKLLARITDGTVSNNTAKKDVFP
AMWAGESDGDADAIIAAKGLKQMSDTGELEKIIDDVLAANAKSVEEFRAGKEKAFNALVGQAMKATKGKANPAQVNELLK
KKLG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=498, Percent_Identity=39.3574297188755, Blast_Score=355, Evalue=5e-98,
Organism=Caenorhabditis elegans, GI115534094, Length=464, Percent_Identity=31.25, Blast_Score=212, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6319391, Length=520, Percent_Identity=30.1923076923077, Blast_Score=214, Evalue=3e-56,
Organism=Drosophila melanogaster, GI45550808, Length=494, Percent_Identity=34.2105263157895, Blast_Score=308, Evalue=7e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52574; Mature: 52574

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQWEVVIGLETHTQLSTASKIFSGTSTAFGAEPNTQASPVDLALPGVLPVLNRGAVERAI
CCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCHHHHHCCHHHHHHH
QFGLSIGATIAPRSIFARKNYFYPDLPKGYQISQYEIPVVQGGQITIQVEGKQGVYEKTV
HHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEECCEEECCEEEEEECCCCCHHHHHH
NLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPDMRSSAEAVAYAKALHSLV
HHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHH
VWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRREIKNLNSFRFLQQAIDYEVQWQIAE
HHHHCCCCCCCCCCEEECCCCEEEECCHHHCCCHHHHHCCHHHHHHHHHHCCEEEEEEEE
IEDGREIVQATVLFDPDTGETRAMRTKEDAHDYRYFPDPDLMPLEIDAAWVERVKSELPE
CCCCCEEEEEEEEECCCCCCCHHHCCHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHCCC
LPATMQARFVSQYGLSAYDASTITATKALAAYYEAVVTNVGAASAKPAANWVMGEIASQL
CCHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
NREGISIDEAPVRPAQLAKLLARITDGTVSNNTAKKDVFPAMWAGESDGDADAIIAAKGL
HHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCHHHCCCCCCCCCHHHHHHHHH
KQMSDTGELEKIIDDVLAANAKSVEEFRAGKEKAFNALVGQAMKATKGKANPAQVNELLK
HHHHCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
KKLG
HHCC
>Mature Secondary Structure
MQWEVVIGLETHTQLSTASKIFSGTSTAFGAEPNTQASPVDLALPGVLPVLNRGAVERAI
CCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCHHHHHCCHHHHHHH
QFGLSIGATIAPRSIFARKNYFYPDLPKGYQISQYEIPVVQGGQITIQVEGKQGVYEKTV
HHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEECCEEECCEEEEEECCCCCHHHHHH
NLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPDMRSSAEAVAYAKALHSLV
HHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHH
VWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRREIKNLNSFRFLQQAIDYEVQWQIAE
HHHHCCCCCCCCCCEEECCCCEEEECCHHHCCCHHHHHCCHHHHHHHHHHCCEEEEEEEE
IEDGREIVQATVLFDPDTGETRAMRTKEDAHDYRYFPDPDLMPLEIDAAWVERVKSELPE
CCCCCEEEEEEEEECCCCCCCHHHCCHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHCCC
LPATMQARFVSQYGLSAYDASTITATKALAAYYEAVVTNVGAASAKPAANWVMGEIASQL
CCHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
NREGISIDEAPVRPAQLAKLLARITDGTVSNNTAKKDVFPAMWAGESDGDADAIIAAKGL
HHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCHHHCCCCCCCCCHHHHHHHHH
KQMSDTGELEKIIDDVLAANAKSVEEFRAGKEKAFNALVGQAMKATKGKANPAQVNELLK
HHHHCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
KKLG
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA