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The map label for this gene is trmE [H]

Identifier: 93007296

GI number: 93007296

Start: 3055907

End: 3057370

Strand: Reverse

Name: trmE [H]

Synonym: Pcryo_2472

Alternate gene names: 93007296

Gene position: 3057370-3055907 (Counterclockwise)

Preceding gene: 93007297

Following gene: 93007295

Centisome position: 99.92

GC content: 46.11

Gene sequence:

>1464_bases
TTGAGAGTGCTTGTGGATTCCCAAGAGTTGGCGTTAGCCGCCCCTGAGTCATCAAAAAATCTCGGTTTGTCGAGTCTGCC
TACCATTGCCGCTATTGCTACACCGCTTGGACGGGGTGGGGTCGGGGTTATTCGTCTGTCTGGTACACATGCGTATAGCA
TTGCTTGTACGCTGACAGGTAAGTCAGCATTTAAACCGCGCATGGCGAGCTTCTGCCGATTTTATCAAGCAGATGGTACG
GTTATCGATGAAGGGTTGGTGCTGTATTTTAAGGGTCCGCACTCGTTTACTGGCGAAGATGTGATTGAACTGCAAGGTCA
TGGTGGCATGATTTTGCAAAATCAGTTGTTGGCACGTGTGTTTGAGTTAGGGGCGAAACAAGCAAGTGCTGGAGAATTCT
CTTATCGTGCCTTTGATAATGATAAGTTGGATTTGGTACAGGCAGAAGCCATTGCAGATGCGATTGATGCTACCAGCGCT
GCTGCTGCCAGCAGTGCTATTCGCTCCTTAAGCGGTGAATTCTCGCAAAAGATTAATCAGCTACTTGAGCAGTTAATTCA
TCTGCGTCTGCATGTTGAAGCAGCTATTGATTTCCCTGATGAAGAAGACGTTGATTTTTTATCAGATGGGGTCATACAAG
GTAAGCTTGAACAGACACAAGAAAAAATCCAGCAGGTCTTGGCGACTGCTAAGCAAGGACAATTATTACGTGATGGTATT
CATGTGGTGTTGGCAGGTAGACCTAATGCTGGTAAATCAAGCCTGCTCAATCGCTTGGCAGGGCAAGAGCGTGCTATCGT
CACTGATGTGGCGGGTACAACGCGTGATACACTACAAGAAACCGTTGTCCTCAATGGTTTGACGCTACACTTGACTGATA
CGGCAGGCCTGCGCGAGACAGAAGACACGGTCGAGCGTATCGGCATTGAGCGCGCGCGGACAGCGATTGCTCAGGCAGAT
ATGCTGTTAATGGTTTATGATGTAACCCGTGATCTTGAAGAAGAAAGTACCCCATTACAGCTCGCTGAGCAGCTATTTGG
CGAGCTACCAGAAGCCAAGCGCTTGTTAATTATCGCTAATAAAAGTGATTTATTAAACAATAATAGCAGCAAAGAAATAA
CCTCTATTTCGCAACAAGAGATTCATAATCGCGGTTACGAGCAAGTTAATGTTTCATGTGAAACAGGCGCAGGTATTGAT
GATTTAGTCGAAACTCTGTGTGCTAAAGTAGGGTTTCATCCACCCGAAAATAGCTTGATAGCTCGTACGCGTCATCTAGA
TGCGTTAAGAAGAACGGCCGAATATTTAGCAGAAGCTCATGAGCAGCTAACCGTCTTTAAAGCGGGTGAATTAGTTGCTG
AAAGTTTGCGTCAAGCTCAGCATAGTCTGGGCGAGATTACCGGGGAGTTTAGTGCAGATGATTTGTTGGGCAAGATTTTT
GGCAGTTTTTGCATTGGTAAATAA

Upstream 100 bases:

>100_bases
GCTAGATGATATTGGAAAAGACTTACAATGAGGAGATTACGCTCTTTAAAGATTGTTTTGTCAGTGTGCGTTAAGCCGTC
TATAATGGAGTTTTTAGTTA

Downstream 100 bases:

>100_bases
TCTTATTTACGGTTATAAATTTGAATGATGTAGCTGTAAAGATGGGATTATGACTCGTTTAATAGTGCTTCATATAAAGA
CAAGGAAAGCCGTATGCATT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MRVLVDSQELALAAPESSKNLGLSSLPTIAAIATPLGRGGVGVIRLSGTHAYSIACTLTGKSAFKPRMASFCRFYQADGT
VIDEGLVLYFKGPHSFTGEDVIELQGHGGMILQNQLLARVFELGAKQASAGEFSYRAFDNDKLDLVQAEAIADAIDATSA
AAASSAIRSLSGEFSQKINQLLEQLIHLRLHVEAAIDFPDEEDVDFLSDGVIQGKLEQTQEKIQQVLATAKQGQLLRDGI
HVVLAGRPNAGKSSLLNRLAGQERAIVTDVAGTTRDTLQETVVLNGLTLHLTDTAGLRETEDTVERIGIERARTAIAQAD
MLLMVYDVTRDLEEESTPLQLAEQLFGELPEAKRLLIIANKSDLLNNNSSKEITSISQQEIHNRGYEQVNVSCETGAGID
DLVETLCAKVGFHPPENSLIARTRHLDALRRTAEYLAEAHEQLTVFKAGELVAESLRQAQHSLGEITGEFSADDLLGKIF
GSFCIGK

Sequences:

>Translated_487_residues
MRVLVDSQELALAAPESSKNLGLSSLPTIAAIATPLGRGGVGVIRLSGTHAYSIACTLTGKSAFKPRMASFCRFYQADGT
VIDEGLVLYFKGPHSFTGEDVIELQGHGGMILQNQLLARVFELGAKQASAGEFSYRAFDNDKLDLVQAEAIADAIDATSA
AAASSAIRSLSGEFSQKINQLLEQLIHLRLHVEAAIDFPDEEDVDFLSDGVIQGKLEQTQEKIQQVLATAKQGQLLRDGI
HVVLAGRPNAGKSSLLNRLAGQERAIVTDVAGTTRDTLQETVVLNGLTLHLTDTAGLRETEDTVERIGIERARTAIAQAD
MLLMVYDVTRDLEEESTPLQLAEQLFGELPEAKRLLIIANKSDLLNNNSSKEITSISQQEIHNRGYEQVNVSCETGAGID
DLVETLCAKVGFHPPENSLIARTRHLDALRRTAEYLAEAHEQLTVFKAGELVAESLRQAQHSLGEITGEFSADDLLGKIF
GSFCIGK
>Mature_487_residues
MRVLVDSQELALAAPESSKNLGLSSLPTIAAIATPLGRGGVGVIRLSGTHAYSIACTLTGKSAFKPRMASFCRFYQADGT
VIDEGLVLYFKGPHSFTGEDVIELQGHGGMILQNQLLARVFELGAKQASAGEFSYRAFDNDKLDLVQAEAIADAIDATSA
AAASSAIRSLSGEFSQKINQLLEQLIHLRLHVEAAIDFPDEEDVDFLSDGVIQGKLEQTQEKIQQVLATAKQGQLLRDGI
HVVLAGRPNAGKSSLLNRLAGQERAIVTDVAGTTRDTLQETVVLNGLTLHLTDTAGLRETEDTVERIGIERARTAIAQAD
MLLMVYDVTRDLEEESTPLQLAEQLFGELPEAKRLLIIANKSDLLNNNSSKEITSISQQEIHNRGYEQVNVSCETGAGID
DLVETLCAKVGFHPPENSLIARTRHLDALRRTAEYLAEAHEQLTVFKAGELVAESLRQAQHSLGEITGEFSADDLLGKIF
GSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=485, Percent_Identity=36.2886597938144, Blast_Score=231, Evalue=2e-60,
Organism=Homo sapiens, GI193082988, Length=513, Percent_Identity=34.6978557504873, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI193082993, Length=480, Percent_Identity=35, Blast_Score=214, Evalue=1e-55,
Organism=Escherichia coli, GI2367268, Length=467, Percent_Identity=51.8201284796574, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI87082120, Length=171, Percent_Identity=29.8245614035088, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17507259, Length=472, Percent_Identity=29.4491525423729, Blast_Score=176, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6323665, Length=497, Percent_Identity=32.3943661971831, Blast_Score=235, Evalue=1e-62,
Organism=Drosophila melanogaster, GI45550813, Length=491, Percent_Identity=32.7902240325866, Blast_Score=224, Evalue=8e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 52645; Mature: 52645

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLVDSQELALAAPESSKNLGLSSLPTIAAIATPLGRGGVGVIRLSGTHAYSIACTLTG
CEEEECCCCCEEECCCCCCCCCCCCCCHHHHHHCCCCCCCEEEEEEECCEEEEEEEEECC
KSAFKPRMASFCRFYQADGTVIDEGLVLYFKGPHSFTGEDVIELQGHGGMILQNQLLARV
CCCCCHHHHHHHHHHHCCCCEEECCEEEEEECCCCCCCCCEEEECCCCCEEHHHHHHHHH
FELGAKQASAGEFSYRAFDNDKLDLVQAEAIADAIDATSAAAASSAIRSLSGEFSQKINQ
HHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLEQLIHLRLHVEAAIDFPDEEDVDFLSDGVIQGKLEQTQEKIQQVLATAKQGQLLRDGI
HHHHHHHHHHEEEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCC
HVVLAGRPNAGKSSLLNRLAGQERAIVTDVAGTTRDTLQETVVLNGLTLHLTDTAGLRET
EEEEECCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCH
EDTVERIGIERARTAIAQADMLLMVYDVTRDLEEESTPLQLAEQLFGELPEAKRLLIIAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEC
KSDLLNNNSSKEITSISQQEIHNRGYEQVNVSCETGAGIDDLVETLCAKVGFHPPENSLI
CHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHH
ARTRHLDALRRTAEYLAEAHEQLTVFKAGELVAESLRQAQHSLGEITGEFSADDLLGKIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
GSFCIGK
HHHHCCC
>Mature Secondary Structure
MRVLVDSQELALAAPESSKNLGLSSLPTIAAIATPLGRGGVGVIRLSGTHAYSIACTLTG
CEEEECCCCCEEECCCCCCCCCCCCCCHHHHHHCCCCCCCEEEEEEECCEEEEEEEEECC
KSAFKPRMASFCRFYQADGTVIDEGLVLYFKGPHSFTGEDVIELQGHGGMILQNQLLARV
CCCCCHHHHHHHHHHHCCCCEEECCEEEEEECCCCCCCCCEEEECCCCCEEHHHHHHHHH
FELGAKQASAGEFSYRAFDNDKLDLVQAEAIADAIDATSAAAASSAIRSLSGEFSQKINQ
HHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLEQLIHLRLHVEAAIDFPDEEDVDFLSDGVIQGKLEQTQEKIQQVLATAKQGQLLRDGI
HHHHHHHHHHEEEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCC
HVVLAGRPNAGKSSLLNRLAGQERAIVTDVAGTTRDTLQETVVLNGLTLHLTDTAGLRET
EEEEECCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCH
EDTVERIGIERARTAIAQADMLLMVYDVTRDLEEESTPLQLAEQLFGELPEAKRLLIIAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEC
KSDLLNNNSSKEITSISQQEIHNRGYEQVNVSCETGAGIDDLVETLCAKVGFHPPENSLI
CHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHH
ARTRHLDALRRTAEYLAEAHEQLTVFKAGELVAESLRQAQHSLGEITGEFSADDLLGKIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
GSFCIGK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA