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The map label for this gene is hisA [H]

Identifier: 93007247

GI number: 93007247

Start: 2995507

End: 2996247

Strand: Reverse

Name: hisA [H]

Synonym: Pcryo_2423

Alternate gene names: 93007247

Gene position: 2996247-2995507 (Counterclockwise)

Preceding gene: 93007261

Following gene: 93007246

Centisome position: 97.92

GC content: 46.56

Gene sequence:

>741_bases
ATGTGCGCTGTCCCTGTGATTATCCCTGCTATCGATCTCAAAGATGGTAAGTGTGTGCGCTTAAAACAAGGTCGTATGGA
AGATGATACGGTATTTTCTGATGACCCTGTGGCGATGGCGGCACGTTGGGTAAAAGAAGGCGCGCGCCGTCTGCATTTGG
TCGATCTAAATGGCGCATTTGATGGGATTCCAGTGCATAAGCAAGTGGTGCATGACATTGCAAAAGCTTTTCCTAAACTG
CCAATTCAATTGGGCGGCGGTGTGCGCAATATGCAAACTATTGAGCAGTATATTACTGCTGGTCTGACCTATATTATTAT
CGGTACCAAAGCGGTTGAAGATCCAGATTTTGTTGCTGAGGCGTGCCGTGAGTTTGCTGGACACATTATCGTTGGTATCG
ATGCCAAAGATGGCATGGTTGCAACCCATGGTTGGGCAAACGTGACTGACACCAAAGCCACAGAGCTTGCCAAACGTTTT
GCTGATGTTGGCGTGTCTTCTATCGTCTATACCGATATCGCCCGTGATGGCATGATGCAAGGCGTTAATGTCGAGCAAAC
CGTTAATTTAGCACGTGAAGGTGGCTTACCAGTGATTGCATCAGGCGGCGTCACCGATATGAAAGATATTGAGTTGTTAA
AACCTTATGGTGATTGCATTGAAGGTATCATTACTGGTCGTGCTATCTATGAGGGCACGTTAGATTTGGGCGAGGCACAA
CTTTATTTGGACGGTAAATAA

Upstream 100 bases:

>100_bases
AGTTCTAGACATTTTATATTAAGTTTGTGTTTAAATGGCTCTTGTAAGCTAACCTACCTCCAAGTTTTGATTATTAATAT
CGTTTTATAAGGAATCCTAT

Downstream 100 bases:

>100_bases
TATTTGAAAAATACGCTTATCAGATTTTGAATAGATAAGGCGTAATCAAGGATGCTTATGGCCACTTATTTTGACTGCAT
TACATCACGCAAGTGCCAAA

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MCAVPVIIPAIDLKDGKCVRLKQGRMEDDTVFSDDPVAMAARWVKEGARRLHLVDLNGAFDGIPVHKQVVHDIAKAFPKL
PIQLGGGVRNMQTIEQYITAGLTYIIIGTKAVEDPDFVAEACREFAGHIIVGIDAKDGMVATHGWANVTDTKATELAKRF
ADVGVSSIVYTDIARDGMMQGVNVEQTVNLAREGGLPVIASGGVTDMKDIELLKPYGDCIEGIITGRAIYEGTLDLGEAQ
LYLDGK

Sequences:

>Translated_246_residues
MCAVPVIIPAIDLKDGKCVRLKQGRMEDDTVFSDDPVAMAARWVKEGARRLHLVDLNGAFDGIPVHKQVVHDIAKAFPKL
PIQLGGGVRNMQTIEQYITAGLTYIIIGTKAVEDPDFVAEACREFAGHIIVGIDAKDGMVATHGWANVTDTKATELAKRF
ADVGVSSIVYTDIARDGMMQGVNVEQTVNLAREGGLPVIASGGVTDMKDIELLKPYGDCIEGIITGRAIYEGTLDLGEAQ
LYLDGK
>Mature_246_residues
MCAVPVIIPAIDLKDGKCVRLKQGRMEDDTVFSDDPVAMAARWVKEGARRLHLVDLNGAFDGIPVHKQVVHDIAKAFPKL
PIQLGGGVRNMQTIEQYITAGLTYIIIGTKAVEDPDFVAEACREFAGHIIVGIDAKDGMVATHGWANVTDTKATELAKRF
ADVGVSSIVYTDIARDGMMQGVNVEQTVNLAREGGLPVIASGGVTDMKDIELLKPYGDCIEGIITGRAIYEGTLDLGEAQ
LYLDGK

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI87082028, Length=245, Percent_Identity=33.0612244897959, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1788336, Length=249, Percent_Identity=26.1044176706827, Blast_Score=81, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: =5.3.1.16 [H]

Molecular weight: Translated: 26467; Mature: 26467

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCAVPVIIPAIDLKDGKCVRLKQGRMEDDTVFSDDPVAMAARWVKEGARRLHLVDLNGAF
CCCCCEEEEEEECCCCCEEEEECCCCCCCCEECCCHHHHHHHHHHHHHHEEEEEEECCCC
DGIPVHKQVVHDIAKAFPKLPIQLGGGVRNMQTIEQYITAGLTYIIIGTKAVEDPDFVAE
CCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHCCEEEEEEECCCCCCHHHHHH
ACREFAGHIIVGIDAKDGMVATHGWANVTDTKATELAKRFADVGVSSIVYTDIARDGMMQ
HHHHHHCEEEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
GVNVEQTVNLAREGGLPVIASGGVTDMKDIELLKPYGDCIEGIITGRAIYEGTLDLGEAQ
CCCHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEE
LYLDGK
EEEECC
>Mature Secondary Structure
MCAVPVIIPAIDLKDGKCVRLKQGRMEDDTVFSDDPVAMAARWVKEGARRLHLVDLNGAF
CCCCCEEEEEEECCCCCEEEEECCCCCCCCEECCCHHHHHHHHHHHHHHEEEEEEECCCC
DGIPVHKQVVHDIAKAFPKLPIQLGGGVRNMQTIEQYITAGLTYIIIGTKAVEDPDFVAE
CCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHCCEEEEEEECCCCCCHHHHHH
ACREFAGHIIVGIDAKDGMVATHGWANVTDTKATELAKRFADVGVSSIVYTDIARDGMMQ
HHHHHHCEEEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
GVNVEQTVNLAREGGLPVIASGGVTDMKDIELLKPYGDCIEGIITGRAIYEGTLDLGEAQ
CCCHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEE
LYLDGK
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA