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The map label for this gene is mscS [H]

Identifier: 93007246

GI number: 93007246

Start: 2993731

End: 2995449

Strand: Reverse

Name: mscS [H]

Synonym: Pcryo_2422

Alternate gene names: 93007246

Gene position: 2995449-2993731 (Counterclockwise)

Preceding gene: 93007247

Following gene: 93007245

Centisome position: 97.89

GC content: 42.58

Gene sequence:

>1719_bases
ATGGCCACTTATTTTGACTGCATTACATCACGCAAGTGCCAAAAAATGTGGAGTCAATGGCTGTTGCCATTAAGCATCCT
ATTAAGTTTACCAGCTTATGCCGCTCAAGAAAGTGAAACTGTTAATCCTATTGTATTAGACAGTAATACTGCCAAGCCCG
TGCTTGATAGCAACGCATTACCTAATGCGGCTAATGACATTAATAGCACCCCTCTTCCTAGTAATACTCCTGAAAGTGGC
ACTTTGCCGGCTCAAGCGCCAGCTGTACCATCTCCGCTAACCCCTATTTCAATTGAACCTGCACCTTCTATCGATGGCGA
GAGCAGTAATAACACTCAAATTGAAACCGCTCAAACTGTGCAACATGATAATGACATCCGCCAACGTATCAGTGGGATAT
TCTCAGAAATTGAAGGCTTGCAAGCAATTAATGTTGGTGTCACTCAAGGCGTTGTTACTTTAACCGGTGAAACACCCAAC
GAGAAAAAAGCCCAACAAGCCATTAATCTTACCAATCGTTTGACAGATGTTGTCACAGTTGAAGATAAGATTAACCGAAC
GCTTGATGTCCAGGATAATGTCACGACGGTTTATCAAAGCATCAAAGCTCAAGCCAAAAACCTTATCAAAGCTGTGCCGT
TATTATTGGTCGGAATTATTTTATTTGCTTTAGTAACTTGGTTTGGTAGTTGGTTGTCCAATCGGCAAAAAATGTGGCAG
CGCTTAACGCCCAATCCTTTTGTCGCAGAATTATTGTCGCAGACCGTCAAAGTCATCTTTATTATTCTGGGGCTTATATT
AGCGTTAAGCCTGATCGGCGCGGAGACGATTTTGGGAACGCTACTTGGCGGCGCTGGGGTCATCGGTATTGCCGTTGGTT
TCGCGGTTAAGGATACTATTGAAAATTATATTGCCTCTTTAATGCTCAGTATTCGCCAGCCATTTCGCGCCCGCGATCAT
ATTTTGATTAATGCTCAAGAGGGAATAGTGGTGCGCCTGACGTCGCGTGCGACTATTTTAATGACGTTAGATGGCAATCA
ATTACGTATTCCCAATGCCGAAGTCTTTAAAGCCACGATTTTAAATTACACCAAAAATCCTGAGCGCCGTTTCACTTTTG
AATTAGGCGTTGATGCCAATGACGATCCGCTTGCTGCCATCAAAGTGGGTATCGATGCGATTGCTCAATTGGATTTTGCC
TTAGATAAGCCAAAAGTCACCGCGATTATCAAGGAGGTAGGCGACTCAAACATTATTTTGCAGTTCCAAGTATGGGTCAA
TCAATCAGAAGCTGATTTTTCCAAAGCCCGCAGTATAGCTATTCGCGAGACCAAGCATGCTTTAGAAGATGAAGGTTTTA
GCTTACCTGAGCCCATTTATCAGTTACGCTTTAACCATAATCTAGAGCAAGCTTTTGAGTATTTCCAAAGCAGTCGAAAT
GGTGATAAGTCTCAAGAAGACATAACAGTAACGCCTATCATCGTTAAACCTTCTAATGATTCTAAAATAGCTAATGATGC
TCAAGCGGCTGAAACTACGATTGAAGATAAAGATAAGCAACAAGCAAAGGCGCGGGCGAAGCATATCTTGCAAGGTCGGA
ATGCCGATCAAGTGCTTGATGCGCGCCCTGACGAAAAGCTAATGGAAAAGGTTGAGCAAGAAATTGCTGAAAATTCAGAT
GAAACTGATTTATTAAGTAATAGTAGCCCGCAAGAATAG

Upstream 100 bases:

>100_bases
GTTAGATTTGGGCGAGGCACAACTTTATTTGGACGGTAAATAATATTTGAAAAATACGCTTATCAGATTTTGAATAGATA
AGGCGTAATCAAGGATGCTT

Downstream 100 bases:

>100_bases
TGCTGAATACCAACTATAAAAATCGGCATAGAAAATGCAGCGAAATGTTTATCTACATTTTATCGAACTAGACGAGACAT
TTTTATGCAAATGAAACATC

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MATYFDCITSRKCQKMWSQWLLPLSILLSLPAYAAQESETVNPIVLDSNTAKPVLDSNALPNAANDINSTPLPSNTPESG
TLPAQAPAVPSPLTPISIEPAPSIDGESSNNTQIETAQTVQHDNDIRQRISGIFSEIEGLQAINVGVTQGVVTLTGETPN
EKKAQQAINLTNRLTDVVTVEDKINRTLDVQDNVTTVYQSIKAQAKNLIKAVPLLLVGIILFALVTWFGSWLSNRQKMWQ
RLTPNPFVAELLSQTVKVIFIILGLILALSLIGAETILGTLLGGAGVIGIAVGFAVKDTIENYIASLMLSIRQPFRARDH
ILINAQEGIVVRLTSRATILMTLDGNQLRIPNAEVFKATILNYTKNPERRFTFELGVDANDDPLAAIKVGIDAIAQLDFA
LDKPKVTAIIKEVGDSNIILQFQVWVNQSEADFSKARSIAIRETKHALEDEGFSLPEPIYQLRFNHNLEQAFEYFQSSRN
GDKSQEDITVTPIIVKPSNDSKIANDAQAAETTIEDKDKQQAKARAKHILQGRNADQVLDARPDEKLMEKVEQEIAENSD
ETDLLSNSSPQE

Sequences:

>Translated_572_residues
MATYFDCITSRKCQKMWSQWLLPLSILLSLPAYAAQESETVNPIVLDSNTAKPVLDSNALPNAANDINSTPLPSNTPESG
TLPAQAPAVPSPLTPISIEPAPSIDGESSNNTQIETAQTVQHDNDIRQRISGIFSEIEGLQAINVGVTQGVVTLTGETPN
EKKAQQAINLTNRLTDVVTVEDKINRTLDVQDNVTTVYQSIKAQAKNLIKAVPLLLVGIILFALVTWFGSWLSNRQKMWQ
RLTPNPFVAELLSQTVKVIFIILGLILALSLIGAETILGTLLGGAGVIGIAVGFAVKDTIENYIASLMLSIRQPFRARDH
ILINAQEGIVVRLTSRATILMTLDGNQLRIPNAEVFKATILNYTKNPERRFTFELGVDANDDPLAAIKVGIDAIAQLDFA
LDKPKVTAIIKEVGDSNIILQFQVWVNQSEADFSKARSIAIRETKHALEDEGFSLPEPIYQLRFNHNLEQAFEYFQSSRN
GDKSQEDITVTPIIVKPSNDSKIANDAQAAETTIEDKDKQQAKARAKHILQGRNADQVLDARPDEKLMEKVEQEIAENSD
ETDLLSNSSPQE
>Mature_571_residues
ATYFDCITSRKCQKMWSQWLLPLSILLSLPAYAAQESETVNPIVLDSNTAKPVLDSNALPNAANDINSTPLPSNTPESGT
LPAQAPAVPSPLTPISIEPAPSIDGESSNNTQIETAQTVQHDNDIRQRISGIFSEIEGLQAINVGVTQGVVTLTGETPNE
KKAQQAINLTNRLTDVVTVEDKINRTLDVQDNVTTVYQSIKAQAKNLIKAVPLLLVGIILFALVTWFGSWLSNRQKMWQR
LTPNPFVAELLSQTVKVIFIILGLILALSLIGAETILGTLLGGAGVIGIAVGFAVKDTIENYIASLMLSIRQPFRARDHI
LINAQEGIVVRLTSRATILMTLDGNQLRIPNAEVFKATILNYTKNPERRFTFELGVDANDDPLAAIKVGIDAIAQLDFAL
DKPKVTAIIKEVGDSNIILQFQVWVNQSEADFSKARSIAIRETKHALEDEGFSLPEPIYQLRFNHNLEQAFEYFQSSRNG
DKSQEDITVTPIIVKPSNDSKIANDAQAAETTIEDKDKQQAKARAKHILQGRNADQVLDARPDEKLMEKVEQEIAENSDE
TDLLSNSSPQE

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=264, Percent_Identity=26.5151515151515, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 62775; Mature: 62644

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50914 BON ; PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATYFDCITSRKCQKMWSQWLLPLSILLSLPAYAAQESETVNPIVLDSNTAKPVLDSNAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCEEECCCCCCCCCCCCCC
PNAANDINSTPLPSNTPESGTLPAQAPAVPSPLTPISIEPAPSIDGESSNNTQIETAQTV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHH
QHDNDIRQRISGIFSEIEGLQAINVGVTQGVVTLTGETPNEKKAQQAINLTNRLTDVVTV
HCCHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHEEE
EDKINRTLDVQDNVTTVYQSIKAQAKNLIKAVPLLLVGIILFALVTWFGSWLSNRQKMWQ
HHHHCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLTPNPFVAELLSQTVKVIFIILGLILALSLIGAETILGTLLGGAGVIGIAVGFAVKDTI
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ENYIASLMLSIRQPFRARDHILINAQEGIVVRLTSRATILMTLDGNQLRIPNAEVFKATI
HHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEECCEEEEEEECCCEEECCCHHHHHHHH
LNYTKNPERRFTFELGVDANDDPLAAIKVGIDAIAQLDFALDKPKVTAIIKEVGDSNIIL
HHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEE
QFQVWVNQSEADFSKARSIAIRETKHALEDEGFSLPEPIYQLRFNHNLEQAFEYFQSSRN
EEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCC
GDKSQEDITVTPIIVKPSNDSKIANDAQAAETTIEDKDKQQAKARAKHILQGRNADQVLD
CCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHC
ARPDEKLMEKVEQEIAENSDETDLLSNSSPQE
CCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCC
>Mature Secondary Structure 
ATYFDCITSRKCQKMWSQWLLPLSILLSLPAYAAQESETVNPIVLDSNTAKPVLDSNAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCEEECCCCCCCCCCCCCC
PNAANDINSTPLPSNTPESGTLPAQAPAVPSPLTPISIEPAPSIDGESSNNTQIETAQTV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHH
QHDNDIRQRISGIFSEIEGLQAINVGVTQGVVTLTGETPNEKKAQQAINLTNRLTDVVTV
HCCHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHEEE
EDKINRTLDVQDNVTTVYQSIKAQAKNLIKAVPLLLVGIILFALVTWFGSWLSNRQKMWQ
HHHHCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLTPNPFVAELLSQTVKVIFIILGLILALSLIGAETILGTLLGGAGVIGIAVGFAVKDTI
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ENYIASLMLSIRQPFRARDHILINAQEGIVVRLTSRATILMTLDGNQLRIPNAEVFKATI
HHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEECCEEEEEEECCCEEECCCHHHHHHHH
LNYTKNPERRFTFELGVDANDDPLAAIKVGIDAIAQLDFALDKPKVTAIIKEVGDSNIIL
HHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEE
QFQVWVNQSEADFSKARSIAIRETKHALEDEGFSLPEPIYQLRFNHNLEQAFEYFQSSRN
EEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCC
GDKSQEDITVTPIIVKPSNDSKIANDAQAAETTIEDKDKQQAKARAKHILQGRNADQVLD
CCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHC
ARPDEKLMEKVEQEIAENSDETDLLSNSSPQE
CCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]