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The map label for this gene is rlmA [H]

Identifier: 93007198

GI number: 93007198

Start: 2918445

End: 2919386

Strand: Reverse

Name: rlmA [H]

Synonym: Pcryo_2374

Alternate gene names: 93007198

Gene position: 2919386-2918445 (Counterclockwise)

Preceding gene: 93007199

Following gene: 93007191

Centisome position: 95.41

GC content: 42.99

Gene sequence:

>942_bases
ATGCCTGTGTCCTTATTTATTTGTCCCCTTTGCCAGTCGCCACTGCAGCCCGCAATCGATACATGGCGCTGTGATGGTAG
CTTGCACCCAAAACAAACTGCGCATCCTTTTGATGTGGCGCGTCAGGGTTACGTTAATCTTCTACCTGTCCAACAAAAAA
AATCTAAAGCACCGGGTGATAGTCAAGCGTCCATCGAGGCACGGAAACGGTTTTTAAATGCAGGGTATTATCAGCCATTA
CAGGCACTGATCTGCCAAAAAATAGTGGAATTGTTAGCAAAAAACGAGTTGCCATCTGAACAAACTGGTAAACCAGTCAA
TTGGCTGGATATCGGCTGCGGCGAAGGCTACTATACCGAAGCGATGGCTCAGACGGGTATGGATACACTTATTGCGGCGG
ATATTAGTAAACCTGCCATAGTAGAGCTTGCAAAAGCGAGCAAGGCAGCAAGTTCTCTTTGGAATCAGCAAGATAAGAAC
GATAGTATTAAAACAGCAGTGATTTATCCATTAGTAACCAGTGCTGCTCATTTGCCCTTGCGTGCGCAAAGGATCAATGG
GATTAGCAGTATTTTTAGTCCCATTCTGCCAGAAGCGTTCAATGAAGTATTAAGCGATGAGGGTTATTTAATCATTGCTA
AGCCAGATATCGGTCATTTAGCAACGATGCGTGAGGCTTTGTTTGACGATGTCCGTGAGCACGATTCTGACAAGTTTTTG
CAGGAATTAGCGCCATATTTTACGCTGCTTAACACTTATAATGTCAGTACTGAGTTAAAATTATCAGCAGATGATTTGGC
TGATTTGCTGACTATGACTCCTTATGCATATCGTGCGCATAGCGAAAAAAGACAAGCGTTACTAGCTTTAGTTGCAACCG
AAACATTTGCAACTGAAGCCAAGTTTGTCATATATATTTTGCAGAAAACAACGAACTTATAA

Upstream 100 bases:

>100_bases
AGATGGCTTTTTTGTTTCCCATACTATCTTTTCTCATACCTTTTAACACTTAGCATGGCTGAATTTTGGTAAAATAACAG
CCATATTGCTATGATTTGAG

Downstream 100 bases:

>100_bases
GTTTACTTATCGCTATGTCATCTTATCAAGACACTTCACAAAATAACCTGATGGGCTTAAAGTAAGAGGCTTATAAAACA
GGACCCATCGTAGCAATGAT

Product: rRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA m1G745 methyltransferase; rRNA (guanine-N(1)-)-methyltransferase [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MPVSLFICPLCQSPLQPAIDTWRCDGSLHPKQTAHPFDVARQGYVNLLPVQQKKSKAPGDSQASIEARKRFLNAGYYQPL
QALICQKIVELLAKNELPSEQTGKPVNWLDIGCGEGYYTEAMAQTGMDTLIAADISKPAIVELAKASKAASSLWNQQDKN
DSIKTAVIYPLVTSAAHLPLRAQRINGISSIFSPILPEAFNEVLSDEGYLIIAKPDIGHLATMREALFDDVREHDSDKFL
QELAPYFTLLNTYNVSTELKLSADDLADLLTMTPYAYRAHSEKRQALLALVATETFATEAKFVIYILQKTTNL

Sequences:

>Translated_313_residues
MPVSLFICPLCQSPLQPAIDTWRCDGSLHPKQTAHPFDVARQGYVNLLPVQQKKSKAPGDSQASIEARKRFLNAGYYQPL
QALICQKIVELLAKNELPSEQTGKPVNWLDIGCGEGYYTEAMAQTGMDTLIAADISKPAIVELAKASKAASSLWNQQDKN
DSIKTAVIYPLVTSAAHLPLRAQRINGISSIFSPILPEAFNEVLSDEGYLIIAKPDIGHLATMREALFDDVREHDSDKFL
QELAPYFTLLNTYNVSTELKLSADDLADLLTMTPYAYRAHSEKRQALLALVATETFATEAKFVIYILQKTTNL
>Mature_312_residues
PVSLFICPLCQSPLQPAIDTWRCDGSLHPKQTAHPFDVARQGYVNLLPVQQKKSKAPGDSQASIEARKRFLNAGYYQPLQ
ALICQKIVELLAKNELPSEQTGKPVNWLDIGCGEGYYTEAMAQTGMDTLIAADISKPAIVELAKASKAASSLWNQQDKND
SIKTAVIYPLVTSAAHLPLRAQRINGISSIFSPILPEAFNEVLSDEGYLIIAKPDIGHLATMREALFDDVREHDSDKFLQ
ELAPYFTLLNTYNVSTELKLSADDLADLLTMTPYAYRAHSEKRQALLALVATETFATEAKFVIYILQKTTNL

Specific function: Specifically methylates the guanosine in position 745 of 23S rRNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=306, Percent_Identity=34.3137254901961, Blast_Score=156, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR016718 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: =2.1.1.187 [H]

Molecular weight: Translated: 34616; Mature: 34485

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVSLFICPLCQSPLQPAIDTWRCDGSLHPKQTAHPFDVARQGYVNLLPVQQKKSKAPGD
CCCEEEECCHHCCCCCCCHHHEECCCCCCCCCCCCHHHHHHCCCEEEEECHHHHCCCCCC
SQASIEARKRFLNAGYYQPLQALICQKIVELLAKNELPSEQTGKPVNWLDIGCGEGYYTE
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCEEECCCCCCHHHH
AMAQTGMDTLIAADISKPAIVELAKASKAASSLWNQQDKNDSIKTAVIYPLVTSAAHLPL
HHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCH
RAQRINGISSIFSPILPEAFNEVLSDEGYLIIAKPDIGHLATMREALFDDVREHDSDKFL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHH
QELAPYFTLLNTYNVSTELKLSADDLADLLTMTPYAYRAHSEKRQALLALVATETFATEA
HHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
KFVIYILQKTTNL
EEEEEEEEHHCCC
>Mature Secondary Structure 
PVSLFICPLCQSPLQPAIDTWRCDGSLHPKQTAHPFDVARQGYVNLLPVQQKKSKAPGD
CCEEEECCHHCCCCCCCHHHEECCCCCCCCCCCCHHHHHHCCCEEEEECHHHHCCCCCC
SQASIEARKRFLNAGYYQPLQALICQKIVELLAKNELPSEQTGKPVNWLDIGCGEGYYTE
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCEEECCCCCCHHHH
AMAQTGMDTLIAADISKPAIVELAKASKAASSLWNQQDKNDSIKTAVIYPLVTSAAHLPL
HHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCH
RAQRINGISSIFSPILPEAFNEVLSDEGYLIIAKPDIGHLATMREALFDDVREHDSDKFL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHH
QELAPYFTLLNTYNVSTELKLSADDLADLLTMTPYAYRAHSEKRQALLALVATETFATEA
HHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
KFVIYILQKTTNL
EEEEEEEEHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7984109; 9097040; 9278503; 9440525 [H]