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The map label for this gene is intB [H]

Identifier: 93007175

GI number: 93007175

Start: 2882520

End: 2883878

Strand: Direct

Name: intB [H]

Synonym: Pcryo_2351

Alternate gene names: 93007175

Gene position: 2882520-2883878 (Clockwise)

Preceding gene: 93007171

Following gene: 93007177

Centisome position: 94.2

GC content: 41.35

Gene sequence:

>1359_bases
ATGTTAAGTGATGCCAAAATTCGTAAGCTTAAGCCAACTGATAAATGCACCCCTTCTCGCCCAGACAAATATAGTGATCA
ACAAGGTTTACAATTACTGGTACGCAGCTCAGGAACGCGGACATGGGTAAGCGCATATCGCTTTGATGGTAAGCAGCAGA
AAACTACTTTAGGTACATACCCACAAATGGGCTTGGCAGAAGCAAGAGTAGCTAACGCTGACATTAAAGCTTTAGTAGCC
AATGGAATCAATCCAAAGAATAAAAAACGTCAAGACAAGCTTGCGAATGAGCAAGCTAAAACGTTCAATGATTATGCGGT
TGAATGGCTAGAGGAGCGTGAGCGCAACGTAAAGCCTCGCACCTATCAGCAAGACTATAATCGCATGCACAAAGACGTTA
TACCTAGCTTTGAAGGCATTGCTTTAAAAGATGTGACGTTTGAAGATTGTAAATTAATGGCAGATAACATTGAAAGCAGG
ACTAATAATGAAGGGGCCTCACCACGAGAAGTAACCAGACGCACTATTGATCTTGTTGGGAATATTCTTAGAAGAGCCAT
GAGAGAGCGCCTAATCGAGGTAAATCATACAGATGGGCTTAAAGAGCTATATCCGAAAGCGAAGACTAAGCACATGAAAC
ACGTTGAGCTTAGCGAGCTACCTAAGCTCTTACAAGACATTGAAGGCTATCATGGTCACGATCAAACTAGATTAGGTATG
AAATTTCTAGCATATTCTTTCTGTCGTACTATCGAGCTAAGAATGATGAAGTGGGAGCATATTGATTTTCCTAACCGTCT
CTGGCGTGTCGATATTGATAATCTTAAAATCGCGCGTAAGCATGTTGTTCCTTTATCAGATCAAATGCTAGCGGTTTTGG
AGGAAATGAAGCACATCACTGGACAGTATGAATACGTTTTCTATCATACTGGTATGCACCAGCCTTATAGCGAAGAGTTC
ATCAATAACGCTTTAGACATTCTCGGATATGGAGGTAAACAAACTGGACATGGCTTCCGTCACATTGCCTCAACTAACCT
AAACGAGCTGGGATACATGGGTGATGCTATTGAAAAGCAGATGGCGCACGACAAGAAAAGTAGCATCAGAGCTGTATATA
ACCATGCTCAACACTTAGAAGAACGACGCAAGATAATGCAAGTATGGGCGGACTTTTTAGACCTACTTAGAGACAAAGGC
GAAGTCATAGACTTCCAGACAGCTCGGCAGCAGATTGAGAAGTCAATGATTGTGGAAAACCAAACATCCCTATCAGATTT
AGATAGTGATGCTTTGATAAAGGCTTTGCAAAATAAAGGTCTAACCGGTGAAGATATAAAATCATTACTATCTCTATAG

Upstream 100 bases:

>100_bases
CAATTAGACCAACATTCGATCTCAATCTATATGGCTTAGACCAACATAATCGTCTATAAGCTTTAAAAATATAGCTTGTA
GGTATAAGGAATAGACCAAC

Downstream 100 bases:

>100_bases
CAAAGGCTAATAAATTTCATTGCCATCCACATCAAGAAATTTTAAATTATATTCCGAGTTAATTAGAATATCGAGCATTG
CAGTTTGAGATAACCTATTA

Product: phage integrase

Products: NA

Alternate protein names: Int(P4) [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MLSDAKIRKLKPTDKCTPSRPDKYSDQQGLQLLVRSSGTRTWVSAYRFDGKQQKTTLGTYPQMGLAEARVANADIKALVA
NGINPKNKKRQDKLANEQAKTFNDYAVEWLEERERNVKPRTYQQDYNRMHKDVIPSFEGIALKDVTFEDCKLMADNIESR
TNNEGASPREVTRRTIDLVGNILRRAMRERLIEVNHTDGLKELYPKAKTKHMKHVELSELPKLLQDIEGYHGHDQTRLGM
KFLAYSFCRTIELRMMKWEHIDFPNRLWRVDIDNLKIARKHVVPLSDQMLAVLEEMKHITGQYEYVFYHTGMHQPYSEEF
INNALDILGYGGKQTGHGFRHIASTNLNELGYMGDAIEKQMAHDKKSSIRAVYNHAQHLEERRKIMQVWADFLDLLRDKG
EVIDFQTARQQIEKSMIVENQTSLSDLDSDALIKALQNKGLTGEDIKSLLSL

Sequences:

>Translated_452_residues
MLSDAKIRKLKPTDKCTPSRPDKYSDQQGLQLLVRSSGTRTWVSAYRFDGKQQKTTLGTYPQMGLAEARVANADIKALVA
NGINPKNKKRQDKLANEQAKTFNDYAVEWLEERERNVKPRTYQQDYNRMHKDVIPSFEGIALKDVTFEDCKLMADNIESR
TNNEGASPREVTRRTIDLVGNILRRAMRERLIEVNHTDGLKELYPKAKTKHMKHVELSELPKLLQDIEGYHGHDQTRLGM
KFLAYSFCRTIELRMMKWEHIDFPNRLWRVDIDNLKIARKHVVPLSDQMLAVLEEMKHITGQYEYVFYHTGMHQPYSEEF
INNALDILGYGGKQTGHGFRHIASTNLNELGYMGDAIEKQMAHDKKSSIRAVYNHAQHLEERRKIMQVWADFLDLLRDKG
EVIDFQTARQQIEKSMIVENQTSLSDLDSDALIKALQNKGLTGEDIKSLLSL
>Mature_452_residues
MLSDAKIRKLKPTDKCTPSRPDKYSDQQGLQLLVRSSGTRTWVSAYRFDGKQQKTTLGTYPQMGLAEARVANADIKALVA
NGINPKNKKRQDKLANEQAKTFNDYAVEWLEERERNVKPRTYQQDYNRMHKDVIPSFEGIALKDVTFEDCKLMADNIESR
TNNEGASPREVTRRTIDLVGNILRRAMRERLIEVNHTDGLKELYPKAKTKHMKHVELSELPKLLQDIEGYHGHDQTRLGM
KFLAYSFCRTIELRMMKWEHIDFPNRLWRVDIDNLKIARKHVVPLSDQMLAVLEEMKHITGQYEYVFYHTGMHQPYSEEF
INNALDILGYGGKQTGHGFRHIASTNLNELGYMGDAIEKQMAHDKKSSIRAVYNHAQHLEERRKIMQVWADFLDLLRDKG
EVIDFQTARQQIEKSMIVENQTSLSDLDSDALIKALQNKGLTGEDIKSLLSL

Specific function: Unknown

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=410, Percent_Identity=30.7317073170732, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1788690, Length=399, Percent_Identity=32.0802005012531, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI145693166, Length=390, Percent_Identity=32.8205128205128, Blast_Score=178, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 52250; Mature: 52250

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSDAKIRKLKPTDKCTPSRPDKYSDQQGLQLLVRSSGTRTWVSAYRFDGKQQKTTLGTY
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCHHCCCCCCC
PQMGLAEARVANADIKALVANGINPKNKKRQDKLANEQAKTFNDYAVEWLEERERNVKPR
CCCCHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TYQQDYNRMHKDVIPSFEGIALKDVTFEDCKLMADNIESRTNNEGASPREVTRRTIDLVG
HHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
NILRRAMRERLIEVNHTDGLKELYPKAKTKHMKHVELSELPKLLQDIEGYHGHDQTRLGM
HHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHH
KFLAYSFCRTIELRMMKWEHIDFPNRLWRVDIDNLKIARKHVVPLSDQMLAVLEEMKHIT
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHC
GQYEYVFYHTGMHQPYSEEFINNALDILGYGGKQTGHGFRHIASTNLNELGYMGDAIEKQ
CCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHH
MAHDKKSSIRAVYNHAQHLEERRKIMQVWADFLDLLRDKGEVIDFQTARQQIEKSMIVEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHCC
QTSLSDLDSDALIKALQNKGLTGEDIKSLLSL
CCHHHHCCHHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MLSDAKIRKLKPTDKCTPSRPDKYSDQQGLQLLVRSSGTRTWVSAYRFDGKQQKTTLGTY
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCHHCCCCCCC
PQMGLAEARVANADIKALVANGINPKNKKRQDKLANEQAKTFNDYAVEWLEERERNVKPR
CCCCHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TYQQDYNRMHKDVIPSFEGIALKDVTFEDCKLMADNIESRTNNEGASPREVTRRTIDLVG
HHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
NILRRAMRERLIEVNHTDGLKELYPKAKTKHMKHVELSELPKLLQDIEGYHGHDQTRLGM
HHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHH
KFLAYSFCRTIELRMMKWEHIDFPNRLWRVDIDNLKIARKHVVPLSDQMLAVLEEMKHIT
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHC
GQYEYVFYHTGMHQPYSEEFINNALDILGYGGKQTGHGFRHIASTNLNELGYMGDAIEKQ
CCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHH
MAHDKKSSIRAVYNHAQHLEERRKIMQVWADFLDLLRDKGEVIDFQTARQQIEKSMIVEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHCC
QTSLSDLDSDALIKALQNKGLTGEDIKSLLSL
CCHHHHCCHHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]