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The map label for this gene is nucA [H]

Identifier: 93007174

GI number: 93007174

Start: 2881029

End: 2881937

Strand: Reverse

Name: nucA [H]

Synonym: Pcryo_2350

Alternate gene names: 93007174

Gene position: 2881937-2881029 (Counterclockwise)

Preceding gene: 93007176

Following gene: 93007173

Centisome position: 94.18

GC content: 42.57

Gene sequence:

>909_bases
ATGGTTGAAAGTTGCATGAGAAGTGTTTTTAAGCGAAGATTTAATCTAAAAAACAGCACGATAACGGTAATAATTGCTAC
CGTTTTAAGCGTATCAACGGCACATGCTGAGGATTTCACACAATGCAGCCAATCATTTTATGGCGGTGTCTATCCTGAAT
TCAGCAATACTAAGCTCAGTAATAATACCCAAGCACTATGTATGGACGGTTTCGCTGTCATGTATTCTGGTATATCACGT
ACACCATTATGGTCAGCAGAATATCTGGATCGTAAGCGTTTACAGCAGGCCAAACAAATCGACCGTGAAGACAGCTTTCA
TGAAGAAAGCCGATTGCCACAGTCCATGCGTGCCAAGCTCTCTGACTATTCAGGCTCAGGTTATGACCGCGGACATTTAG
CGCCTAATGGTGACATGGCCAATCGAAGTCAGCAGTATGATAGCTTTAGCCTTGCTAATATAGCCCCGCAATCACCACGT
AATAATCGCTATATCTGGCGCAATATTGAATCGGCGACTCGCTATTTAACGCAGCAATATGGTGAAGTCTATACTGTCAC
AGGGGTTGCATTTACTGATAAAAAAGCTAAGCAACTAGCAGGGCGAGTACTGGTGCCCAGTCATTTCTTTAAAGCCGTTT
ATATCCCAGCGATCAATCAAGCAGGTGTTTACTATGCACCAAACGACGAATCTGAGCGTATAGAAATAATTAGTATTGAC
GAATTGACGTCTAAAATTGGTATTGACGTCTTGCCGGTACTTGATGCAAAAACTAAAGTACAAGCTCTGGATTTACCGCT
AAAGGCTGGTGAAGCGATAGATCTACCAACCACAGAGGTAGAAGAGCCTGCTTGGATACTATTCGTAGTAGCAATTTTTG
AATGGCTCATGGCACAATTGCAAACATAG

Upstream 100 bases:

>100_bases
CTAGTATAGCGGATAGCATTTAAAATGTCACGGTGTGCGCAGTATCTTTCTTTATTGGTAGACGCCTGCTAGGATAGAGC
ATACGTAATAGTAAGCATAA

Downstream 100 bases:

>100_bases
ATCGTTTAAATATTGCTGGACCTGTGTGATTCTGATACATCCTGTAATATGCTACTTATGAAAAAAGGGGGATTAAATGA
TTGACCCATTTGCTAATAAC

Product: DNA/RNA non-specific endonuclease

Products: NA

Alternate protein names: Endonuclease [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MVESCMRSVFKRRFNLKNSTITVIIATVLSVSTAHAEDFTQCSQSFYGGVYPEFSNTKLSNNTQALCMDGFAVMYSGISR
TPLWSAEYLDRKRLQQAKQIDREDSFHEESRLPQSMRAKLSDYSGSGYDRGHLAPNGDMANRSQQYDSFSLANIAPQSPR
NNRYIWRNIESATRYLTQQYGEVYTVTGVAFTDKKAKQLAGRVLVPSHFFKAVYIPAINQAGVYYAPNDESERIEIISID
ELTSKIGIDVLPVLDAKTKVQALDLPLKAGEAIDLPTTEVEEPAWILFVVAIFEWLMAQLQT

Sequences:

>Translated_302_residues
MVESCMRSVFKRRFNLKNSTITVIIATVLSVSTAHAEDFTQCSQSFYGGVYPEFSNTKLSNNTQALCMDGFAVMYSGISR
TPLWSAEYLDRKRLQQAKQIDREDSFHEESRLPQSMRAKLSDYSGSGYDRGHLAPNGDMANRSQQYDSFSLANIAPQSPR
NNRYIWRNIESATRYLTQQYGEVYTVTGVAFTDKKAKQLAGRVLVPSHFFKAVYIPAINQAGVYYAPNDESERIEIISID
ELTSKIGIDVLPVLDAKTKVQALDLPLKAGEAIDLPTTEVEEPAWILFVVAIFEWLMAQLQT
>Mature_302_residues
MVESCMRSVFKRRFNLKNSTITVIIATVLSVSTAHAEDFTQCSQSFYGGVYPEFSNTKLSNNTQALCMDGFAVMYSGISR
TPLWSAEYLDRKRLQQAKQIDREDSFHEESRLPQSMRAKLSDYSGSGYDRGHLAPNGDMANRSQQYDSFSLANIAPQSPR
NNRYIWRNIESATRYLTQQYGEVYTVTGVAFTDKKAKQLAGRVLVPSHFFKAVYIPAINQAGVYYAPNDESERIEIISID
ELTSKIGIDVLPVLDAKTKVQALDLPLKAGEAIDLPTTEVEEPAWILFVVAIFEWLMAQLQT

Specific function: Catalyzes the degradation of both RNA and DNA; has the potential to act as an endonuclease [H]

COG id: COG1864

COG function: function code F; DNA/RNA endonuclease G, NUC1

Gene ontology:

Cell location: Periplasm. Note=Periplasmic or loosely attached to the cytoplasmic or the outer membrane [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA/RNA non-specific endonuclease family [H]

Homologues:

Organism=Homo sapiens, GI53759134, Length=225, Percent_Identity=31.5555555555556, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI224451073, Length=225, Percent_Identity=27.1111111111111, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI224451075, Length=180, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17505967, Length=232, Percent_Identity=29.3103448275862, Blast_Score=87, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6322253, Length=210, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24581065, Length=159, Percent_Identity=30.8176100628931, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI20129899, Length=156, Percent_Identity=30.7692307692308, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018524
- InterPro:   IPR001604
- InterPro:   IPR020821 [H]

Pfam domain/function: PF01223 Endonuclease_NS [H]

EC number: 3.1.30.-

Molecular weight: Translated: 34014; Mature: 34014

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS01070 NUCLEASE_NON_SPEC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVESCMRSVFKRRFNLKNSTITVIIATVLSVSTAHAEDFTQCSQSFYGGVYPEFSNTKLS
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
NNTQALCMDGFAVMYSGISRTPLWSAEYLDRKRLQQAKQIDREDSFHEESRLPQSMRAKL
CCCCEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHH
SDYSGSGYDRGHLAPNGDMANRSQQYDSFSLANIAPQSPRNNRYIWRNIESATRYLTQQY
HCCCCCCCCCCCCCCCCCCCCCHHHCCCEEHHCCCCCCCCCCCEEEECHHHHHHHHHHHH
GEVYTVTGVAFTDKKAKQLAGRVLVPSHFFKAVYIPAINQAGVYYAPNDESERIEIISID
CCEEEEEEEEECCHHHHHHHCCCCCCHHHHHEEEECCCCCCCEEECCCCCCCEEEEEEHH
ELTSKIGIDVLPVLDAKTKVQALDLPLKAGEAIDLPTTEVEEPAWILFVVAIFEWLMAQL
HHHHHHCCEEEEECCCCCHHHEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
QT
CC
>Mature Secondary Structure
MVESCMRSVFKRRFNLKNSTITVIIATVLSVSTAHAEDFTQCSQSFYGGVYPEFSNTKLS
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
NNTQALCMDGFAVMYSGISRTPLWSAEYLDRKRLQQAKQIDREDSFHEESRLPQSMRAKL
CCCCEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHH
SDYSGSGYDRGHLAPNGDMANRSQQYDSFSLANIAPQSPRNNRYIWRNIESATRYLTQQY
HCCCCCCCCCCCCCCCCCCCCCHHHCCCEEHHCCCCCCCCCCCEEEECHHHHHHHHHHHH
GEVYTVTGVAFTDKKAKQLAGRVLVPSHFFKAVYIPAINQAGVYYAPNDESERIEIISID
CCEEEEEEEEECCHHHHHHHCCCCCCHHHHHEEEECCCCCCCEEECCCCCCCEEEEEEHH
ELTSKIGIDVLPVLDAKTKVQALDLPLKAGEAIDLPTTEVEEPAWILFVVAIFEWLMAQL
HHHHHHCCEEEEECCCCCHHHEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
QT
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1343821; 11759840 [H]