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The map label for this gene is gluQ [H]

Identifier: 93007165

GI number: 93007165

Start: 2870543

End: 2871571

Strand: Direct

Name: gluQ [H]

Synonym: Pcryo_2341

Alternate gene names: 93007165

Gene position: 2870543-2871571 (Clockwise)

Preceding gene: 93007164

Following gene: 93007168

Centisome position: 93.81

GC content: 42.08

Gene sequence:

>1029_bases
ATGTTGAAAACGTTTACCCCGTCTAAGTCAGAAAAAACCATGCCAACCAATATTGAATTGACGCAGCCTATCGGCCGTTT
TGCGCCTTCACCCACAGGTGAGCTGCATCTAGGTTCGTTAACGACCGCGCTTGCCAGCTTTTGTCATATTAAATCACTCG
GTGGCAAATGGTTATTACGTATCGAAGATACGGACACCGAACGTTGTGACAGACAGTTTACCGAGCAAATTTTGATAGAT
TTAGAGGCGCTTGGGCTCAACTGGGATGACGATGTTATTTTTCAATCTGAGCGCATTGATATTTATAATGATTATGTCTG
TTCAGTATTAAGTCCTTTGACTTATGGCTGTCAGTGCTCACGTAAGCGTTTAGAGCATTATTGGACACAGCAAGAACAAC
AAATAGCGCTTAATGATGTAAAATTATCCACTGACTTGTCTATTAACGATAATAGAAAAGTAGATAGTGATCTACAGCCC
AATAGGCAACGCTATCCACGTTTTTGTATTGAGAAGGATTTAAGTCCTATACAACATAAACTGCGTATACAGCTGCCAGA
TTACTGTATTGGATTTAATGATGGTATTCAGGGCATACAGTGGGATAATCCGCAGCAAACTTTAGGGGACATGGTGGCTC
GACGTCAAGATGGCATGATTAATTATATTTTGGCGGCAAGTCTTGATGATGGACTACAGCAAGTCACCCATATTATGCGC
GGTTTGGACATCTTACCGATGACGACTGCTCAAATCAGTATCATGCAAGCAGCACGCTTACCGACGATCAGTCATTGGTA
TCATCTGCCTTTGATATGCAATTCAGATGGTCAAAAACTCTCTAAGCAAAATCTTGCGCAGCCTATCGATACCCGTTATC
CAAGTGAATTACTTGCGTATGCGTTAAAGCTATTAGGGCAGTTACCAGTCGAGCATGACTCGCCAGAGCGTATGCTAAAT
CAAGCAATTGCTCAATGGGATAACACGCCATTACAAGGTAGGCAGTCTTTAAATATGCCCGCTATTTAA

Upstream 100 bases:

>100_bases
TTGACGAGTCACCAACAAAATAAGCGTATTTGTTAAATATCTTTTTATAGGTGGATTGTTTATTCTATTCATCTCTCTAG
TCATTTTACAATAACTGTCT

Downstream 100 bases:

>100_bases
CAAAAAAACGCCGCTATAAGCGACGCTTCTCTAAATCAAAAACACCAATTAGTAATAGCGACATTGGCTTTTTTCAATCT
CAGGACTTTCTTTATAAATC

Product: glutamate--tRNA ligase

Products: NA

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MLKTFTPSKSEKTMPTNIELTQPIGRFAPSPTGELHLGSLTTALASFCHIKSLGGKWLLRIEDTDTERCDRQFTEQILID
LEALGLNWDDDVIFQSERIDIYNDYVCSVLSPLTYGCQCSRKRLEHYWTQQEQQIALNDVKLSTDLSINDNRKVDSDLQP
NRQRYPRFCIEKDLSPIQHKLRIQLPDYCIGFNDGIQGIQWDNPQQTLGDMVARRQDGMINYILAASLDDGLQQVTHIMR
GLDILPMTTAQISIMQAARLPTISHWYHLPLICNSDGQKLSKQNLAQPIDTRYPSELLAYALKLLGQLPVEHDSPERMLN
QAIAQWDNTPLQGRQSLNMPAI

Sequences:

>Translated_342_residues
MLKTFTPSKSEKTMPTNIELTQPIGRFAPSPTGELHLGSLTTALASFCHIKSLGGKWLLRIEDTDTERCDRQFTEQILID
LEALGLNWDDDVIFQSERIDIYNDYVCSVLSPLTYGCQCSRKRLEHYWTQQEQQIALNDVKLSTDLSINDNRKVDSDLQP
NRQRYPRFCIEKDLSPIQHKLRIQLPDYCIGFNDGIQGIQWDNPQQTLGDMVARRQDGMINYILAASLDDGLQQVTHIMR
GLDILPMTTAQISIMQAARLPTISHWYHLPLICNSDGQKLSKQNLAQPIDTRYPSELLAYALKLLGQLPVEHDSPERMLN
QAIAQWDNTPLQGRQSLNMPAI
>Mature_342_residues
MLKTFTPSKSEKTMPTNIELTQPIGRFAPSPTGELHLGSLTTALASFCHIKSLGGKWLLRIEDTDTERCDRQFTEQILID
LEALGLNWDDDVIFQSERIDIYNDYVCSVLSPLTYGCQCSRKRLEHYWTQQEQQIALNDVKLSTDLSINDNRKVDSDLQP
NRQRYPRFCIEKDLSPIQHKLRIQLPDYCIGFNDGIQGIQWDNPQQTLGDMVARRQDGMINYILAASLDDGLQQVTHIMR
GLDILPMTTAQISIMQAARLPTISHWYHLPLICNSDGQKLSKQNLAQPIDTRYPSELLAYALKLLGQLPVEHDSPERMLN
QAIAQWDNTPLQGRQSLNMPAI

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=267, Percent_Identity=31.4606741573034, Blast_Score=102, Evalue=5e-22,
Organism=Escherichia coli, GI226510925, Length=315, Percent_Identity=34.9206349206349, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI1788743, Length=286, Percent_Identity=33.9160839160839, Blast_Score=141, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI71993394, Length=295, Percent_Identity=30.5084745762712, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324540, Length=273, Percent_Identity=32.967032967033, Blast_Score=115, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24665321, Length=274, Percent_Identity=34.3065693430657, Blast_Score=116, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: NA

Molecular weight: Translated: 38992; Mature: 38992

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKTFTPSKSEKTMPTNIELTQPIGRFAPSPTGELHLGSLTTALASFCHIKSLGGKWLLR
CCCCCCCCCCCCCCCCCCEECCCHHHCCCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEE
IEDTDTERCDRQFTEQILIDLEALGLNWDDDVIFQSERIDIYNDYVCSVLSPLTYGCQCS
EECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCHHHH
RKRLEHYWTQQEQQIALNDVKLSTDLSINDNRKVDSDLQPNRQRYPRFCIEKDLSPIQHK
HHHHHHHHCCHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCHHHCCHHHHHHCCHHHHHH
LRIQLPDYCIGFNDGIQGIQWDNPQQTLGDMVARRQDGMINYILAASLDDGLQQVTHIMR
EEEECCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHH
GLDILPMTTAQISIMQAARLPTISHWYHLPLICNSDGQKLSKQNLAQPIDTRYPSELLAY
CCCCCCCCHHHHHHHHHHCCCCHHCEEEEEEEECCCCHHHHHHHCCCCCCCCCHHHHHHH
ALKLLGQLPVEHDSPERMLNQAIAQWDNTPLQGRQSLNMPAI
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLKTFTPSKSEKTMPTNIELTQPIGRFAPSPTGELHLGSLTTALASFCHIKSLGGKWLLR
CCCCCCCCCCCCCCCCCCEECCCHHHCCCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEE
IEDTDTERCDRQFTEQILIDLEALGLNWDDDVIFQSERIDIYNDYVCSVLSPLTYGCQCS
EECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCHHHH
RKRLEHYWTQQEQQIALNDVKLSTDLSINDNRKVDSDLQPNRQRYPRFCIEKDLSPIQHK
HHHHHHHHCCHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCHHHCCHHHHHHCCHHHHHH
LRIQLPDYCIGFNDGIQGIQWDNPQQTLGDMVARRQDGMINYILAASLDDGLQQVTHIMR
EEEECCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHH
GLDILPMTTAQISIMQAARLPTISHWYHLPLICNSDGQKLSKQNLAQPIDTRYPSELLAY
CCCCCCCCHHHHHHHHHHCCCCHHCEEEEEEEECCCCHHHHHHHCCCCCCCCCHHHHHHH
ALKLLGQLPVEHDSPERMLNQAIAQWDNTPLQGRQSLNMPAI
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA