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The map label for this gene is rstA [H]

Identifier: 93007149

GI number: 93007149

Start: 2854989

End: 2855705

Strand: Reverse

Name: rstA [H]

Synonym: Pcryo_2325

Alternate gene names: 93007149

Gene position: 2855705-2854989 (Counterclockwise)

Preceding gene: 93007150

Following gene: 93007148

Centisome position: 93.33

GC content: 45.33

Gene sequence:

>717_bases
ATGACAACGAATGATGATGACAACACCCCCCGAATTTTGATTGTTGAGGACGATGAGCGTCTGGCCATGTTGACCCAAGA
TTATTTGGTCAAAAACGGTCTAGAAGTGGCGATTGAAACGGATGGTAATCGCGCCATTCGTCGTATTGTCAGTGAGCAGC
CAGATTTGGTTGTACTTGATGTCATGCTTCCAGGTAGCGATGGCTTGACAGTCTGTCGTGAAGTACGTCCACATTACCAA
AACCCTATCTTAATGCTGACGGCTCGTACTGAAGATATGGATCAAGTATTGGGTCTTGAGATGGGCGCCGACGATTATGT
TGCTAAGCCTGCTCAGCCACGTGTTTTATTAGCGCGTATTCGTGCGTTATTGCGCCGTTCAGAAAACGCCCCGTCAGAAG
ATGTGCCACAGCGTCTAGAGTTTGGTGAATTGGTTATCGATAATGGTGGTCGTTCAGTGAATCTAGGTGATGAGTTAGTA
GATTTCACCAGTGCAGAATATGATCTACTATGGTTGCTTGCTTCTAATGCTGGTCGTATTCTATCTCGCGAAGATATCTT
TGAGCGTCTACGTGGTATTGAGTACGATGGTCAAGATCGCTCTATTGACGTCCGTATCTCACGCATTCGTCCAAAAATCG
GTGATGATCCAGAAAACCCAAAACGTATCAAAACCGTACGTAGTAAAGGCTATCTGTTCGTAAAAGAAGGCAACTAA

Upstream 100 bases:

>100_bases
AACAGCTATATAAACAGCAGTTGCACTATTTTGTAGTGCTATTCATTTTACTGCTCTTATTTTGACTACTTTTAAATGAG
ATGTATTAGAGGATATAACA

Downstream 100 bases:

>100_bases
TCGCCGACTTTTGCTATAAGTCTTGATTGTTCCTCGTTATGCAATAAGAAACCAGCGTAAAGCTGGTTTTTTAGGGTTTA
AATATTAAGTTTTTAACCTT

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MTTNDDDNTPRILIVEDDERLAMLTQDYLVKNGLEVAIETDGNRAIRRIVSEQPDLVVLDVMLPGSDGLTVCREVRPHYQ
NPILMLTARTEDMDQVLGLEMGADDYVAKPAQPRVLLARIRALLRRSENAPSEDVPQRLEFGELVIDNGGRSVNLGDELV
DFTSAEYDLLWLLASNAGRILSREDIFERLRGIEYDGQDRSIDVRISRIRPKIGDDPENPKRIKTVRSKGYLFVKEGN

Sequences:

>Translated_238_residues
MTTNDDDNTPRILIVEDDERLAMLTQDYLVKNGLEVAIETDGNRAIRRIVSEQPDLVVLDVMLPGSDGLTVCREVRPHYQ
NPILMLTARTEDMDQVLGLEMGADDYVAKPAQPRVLLARIRALLRRSENAPSEDVPQRLEFGELVIDNGGRSVNLGDELV
DFTSAEYDLLWLLASNAGRILSREDIFERLRGIEYDGQDRSIDVRISRIRPKIGDDPENPKRIKTVRSKGYLFVKEGN
>Mature_237_residues
TTNDDDNTPRILIVEDDERLAMLTQDYLVKNGLEVAIETDGNRAIRRIVSEQPDLVVLDVMLPGSDGLTVCREVRPHYQN
PILMLTARTEDMDQVLGLEMGADDYVAKPAQPRVLLARIRALLRRSENAPSEDVPQRLEFGELVIDNGGRSVNLGDELVD
FTSAEYDLLWLLASNAGRILSREDIFERLRGIEYDGQDRSIDVRISRIRPKIGDDPENPKRIKTVRSKGYLFVKEGN

Specific function: Member of the two-component regulatory system rstB/rstA [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI145693140, Length=234, Percent_Identity=45.2991452991453, Blast_Score=196, Evalue=8e-52,
Organism=Escherichia coli, GI1789809, Length=233, Percent_Identity=42.9184549356223, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1788394, Length=226, Percent_Identity=38.9380530973451, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI2367329, Length=228, Percent_Identity=37.719298245614, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI87082012, Length=224, Percent_Identity=37.5, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=34.4978165938865, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1790863, Length=232, Percent_Identity=34.9137931034483, Blast_Score=127, Evalue=5e-31,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=30.0448430493274, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1786784, Length=224, Percent_Identity=34.375, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1789402, Length=222, Percent_Identity=36.4864864864865, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1790860, Length=221, Percent_Identity=33.0316742081448, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1790552, Length=234, Percent_Identity=35.8974358974359, Blast_Score=110, Evalue=6e-26,
Organism=Escherichia coli, GI1787229, Length=231, Percent_Identity=31.6017316017316, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=32.8888888888889, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1788550, Length=101, Percent_Identity=32.6732673267327, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26918; Mature: 26787

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNDDDNTPRILIVEDDERLAMLTQDYLVKNGLEVAIETDGNRAIRRIVSEQPDLVVLD
CCCCCCCCCCEEEEEECCCCEEHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCEEEEE
VMLPGSDGLTVCREVRPHYQNPILMLTARTEDMDQVLGLEMGADDYVAKPAQPRVLLARI
EEECCCCCHHHHHHHCCCCCCCEEEEEECCCCHHHHHCCCCCCCHHHCCCCCCHHHHHHH
RALLRRSENAPSEDVPQRLEFGELVIDNGGRSVNLGDELVDFTSAEYDLLWLLASNAGRI
HHHHHHCCCCCCCHHHHHHCCCEEEEECCCCEEECCHHHHHHCCCCEEEEEEEECCCCCC
LSREDIFERLRGIEYDGQDRSIDVRISRIRPKIGDDPENPKRIKTVRSKGYLFVKEGN
CCHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHCCCEEEEECCC
>Mature Secondary Structure 
TTNDDDNTPRILIVEDDERLAMLTQDYLVKNGLEVAIETDGNRAIRRIVSEQPDLVVLD
CCCCCCCCCEEEEEECCCCEEHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCEEEEE
VMLPGSDGLTVCREVRPHYQNPILMLTARTEDMDQVLGLEMGADDYVAKPAQPRVLLARI
EEECCCCCHHHHHHHCCCCCCCEEEEEECCCCHHHHHCCCCCCCHHHCCCCCCHHHHHHH
RALLRRSENAPSEDVPQRLEFGELVIDNGGRSVNLGDELVDFTSAEYDLLWLLASNAGRI
HHHHHHCCCCCCCHHHHHHCCCEEEEECCCCEEECCHHHHHHCCCCEEEEEEEECCCCCC
LSREDIFERLRGIEYDGQDRSIDVRISRIRPKIGDDPENPKRIKTVRSKGYLFVKEGN
CCHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1495392 [H]