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The map label for this gene is rstB [H]

Identifier: 93007148

GI number: 93007148

Start: 2853058

End: 2854743

Strand: Reverse

Name: rstB [H]

Synonym: Pcryo_2324

Alternate gene names: 93007148

Gene position: 2854743-2853058 (Counterclockwise)

Preceding gene: 93007149

Following gene: 93007146

Centisome position: 93.3

GC content: 43.53

Gene sequence:

>1686_bases
ATGTCATTAGTCTCTTCTCTTAAACACAGTATTTTTGTTCGTATTTATGCAGGGCTATTGATAGTCTGCTTGTGTGTGGC
ACTATTTGCCCAGCTATTGATGGATACCATCAATAAAGAGCGTGTCCAATCTTATCGTGAAAATATGGCGACAGGGGCGT
TTCATATCGTGAGTGAGGGTTTGGCTCACCAAGAAAACGAGGCGCAACGTGATTATTGGTTGTCTGATGCCAGTAGTTTG
TTTGGCTCAGAATTTAAGATCGTGTCTATCGATGAAGTTGATTTTAATGCCAGCGAGCTGCGCCGTTTTGAGAAAGAAAA
CACGGTCGTACGCTATATCAGTCAACTGACCACTGCTGATGTCTATTATCGCCTACCAGACAATCAAGGTGTTTTGACTG
CGCGTATCTCACAAGTGACTGAACAGCAAGTCAGAGCTATCGCCGTATTTTTGCTAGATGATTTGTCCTATTATATTACT
TTGTCTGATAAACGTGCACGTTTGGCACAGTTAGAAGAAAAGTTTTCATTTCCTTTAGCATTTAAAGCAGTAGCGGCGCT
CAATTTGGATCCTGATCAGATGGCGCGCTTGCGCCGTGATGAGGTGGTTATCTTATTGCAAGATACCAATACCAGTAAAA
GTAACTCATCTATCAAAGTCATTGTGCCGTCTGAAATTAGTGATATGGCAATTTTGATCGGTCCAGTACCTTTATTTAAT
TGGTTCCCACTAAACTTGATCGTCAGTATGATTTTGATCAGTATGTTTTTAATTAGTTTGGGTGTGTATGCACTGATTTT
CCCGCTTGAGCGCAAGTTACAACTGATACAGGTCGGGGTGAATGAGGTCAGTAAAGGTAATCTTGAAAGTCAAGTACAGG
TGATTGGTCAAGATGAAATCGCCCGTTTAGCTGCAACCTTTAATGCCATGACTTCTCATATTAAGCGTTTGATTGAGTCG
CAGCGAGAGCTAACGCGAGCGGTGTCACATGAGCTACGTACGCCCGTTGCACGTATTCGCTTTGCTGTTGATATGCTGGC
AGATACTGATGATGAAGATTCGCGTTTTATGCAGCGTGACTTTATTGATGAAGATATCGAAGCGCTTAATGGCTTGATTG
ATGAAATTCTCACCTATGCGAAACTTGAAGAAGGCTCGCCCAAACTAGATTTGGAACCAGTCAATCTTCGAGAGCTGCTT
GAGCAGATTGAGCGTGAAACCAATGCCTTGGGTAAACCGATTAAAATTGTCACCAAACTGCCAAATGCTAAAGTAGAGGC
GATAGCAGATAGACGCTACTTACATCGAGTGATTCAAAATTTGGCTGGTAATGCTCTGCGTTATGCGGAGACTACGATTA
TCATTAGTGCTGGGGTCAAAAAGGGCAATGCCTTTGTCAGCGTTGAAGATGATGGTCACGGTATTCCAGAAGCAGATCGC
GAAAAGGTCTTTATTCCATTCTCCCGCTTAGACGATAGTCGGACCCGTGCCTCAGGTGGCTACGGTCTGGGCTTGTCTAT
CGTATCACGCATTGCTTTTTGGTTTAATGGTGGTATGAAGGTTGATGAGAGCCGAGATCTTGGTGGCGCGCGGTTTGTGA
TGACATGGCCTATCAAGCCACTGACACAAATTCTAGCAGCCGATGAATTAACCCAAGATAAGAGTGAGCGTGAGTTTGAT
GCATAA

Upstream 100 bases:

>100_bases
AGTTTAATTATCATATAAATATATATCACAGCAAATATTATCCTATAATAGGTATTATATCTATTGATAACGCTTATTCA
TCAGGTTGGTCACGGCACGT

Downstream 100 bases:

>100_bases
GCATCGTTAATATACTTTTGAAATAAAACAGTGCTGTTAAGATGACTTTTATAAAGGTATCAAAATGTCTATCGAAATAC
TATTTCACTTGTAGCTATGT

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MSLVSSLKHSIFVRIYAGLLIVCLCVALFAQLLMDTINKERVQSYRENMATGAFHIVSEGLAHQENEAQRDYWLSDASSL
FGSEFKIVSIDEVDFNASELRRFEKENTVVRYISQLTTADVYYRLPDNQGVLTARISQVTEQQVRAIAVFLLDDLSYYIT
LSDKRARLAQLEEKFSFPLAFKAVAALNLDPDQMARLRRDEVVILLQDTNTSKSNSSIKVIVPSEISDMAILIGPVPLFN
WFPLNLIVSMILISMFLISLGVYALIFPLERKLQLIQVGVNEVSKGNLESQVQVIGQDEIARLAATFNAMTSHIKRLIES
QRELTRAVSHELRTPVARIRFAVDMLADTDDEDSRFMQRDFIDEDIEALNGLIDEILTYAKLEEGSPKLDLEPVNLRELL
EQIERETNALGKPIKIVTKLPNAKVEAIADRRYLHRVIQNLAGNALRYAETTIIISAGVKKGNAFVSVEDDGHGIPEADR
EKVFIPFSRLDDSRTRASGGYGLGLSIVSRIAFWFNGGMKVDESRDLGGARFVMTWPIKPLTQILAADELTQDKSEREFD
A

Sequences:

>Translated_561_residues
MSLVSSLKHSIFVRIYAGLLIVCLCVALFAQLLMDTINKERVQSYRENMATGAFHIVSEGLAHQENEAQRDYWLSDASSL
FGSEFKIVSIDEVDFNASELRRFEKENTVVRYISQLTTADVYYRLPDNQGVLTARISQVTEQQVRAIAVFLLDDLSYYIT
LSDKRARLAQLEEKFSFPLAFKAVAALNLDPDQMARLRRDEVVILLQDTNTSKSNSSIKVIVPSEISDMAILIGPVPLFN
WFPLNLIVSMILISMFLISLGVYALIFPLERKLQLIQVGVNEVSKGNLESQVQVIGQDEIARLAATFNAMTSHIKRLIES
QRELTRAVSHELRTPVARIRFAVDMLADTDDEDSRFMQRDFIDEDIEALNGLIDEILTYAKLEEGSPKLDLEPVNLRELL
EQIERETNALGKPIKIVTKLPNAKVEAIADRRYLHRVIQNLAGNALRYAETTIIISAGVKKGNAFVSVEDDGHGIPEADR
EKVFIPFSRLDDSRTRASGGYGLGLSIVSRIAFWFNGGMKVDESRDLGGARFVMTWPIKPLTQILAADELTQDKSEREFD
A
>Mature_560_residues
SLVSSLKHSIFVRIYAGLLIVCLCVALFAQLLMDTINKERVQSYRENMATGAFHIVSEGLAHQENEAQRDYWLSDASSLF
GSEFKIVSIDEVDFNASELRRFEKENTVVRYISQLTTADVYYRLPDNQGVLTARISQVTEQQVRAIAVFLLDDLSYYITL
SDKRARLAQLEEKFSFPLAFKAVAALNLDPDQMARLRRDEVVILLQDTNTSKSNSSIKVIVPSEISDMAILIGPVPLFNW
FPLNLIVSMILISMFLISLGVYALIFPLERKLQLIQVGVNEVSKGNLESQVQVIGQDEIARLAATFNAMTSHIKRLIESQ
RELTRAVSHELRTPVARIRFAVDMLADTDDEDSRFMQRDFIDEDIEALNGLIDEILTYAKLEEGSPKLDLEPVNLRELLE
QIERETNALGKPIKIVTKLPNAKVEAIADRRYLHRVIQNLAGNALRYAETTIIISAGVKKGNAFVSVEDDGHGIPEADRE
KVFIPFSRLDDSRTRASGGYGLGLSIVSRIAFWFNGGMKVDESRDLGGARFVMTWPIKPLTQILAADELTQDKSEREFDA

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=404, Percent_Identity=33.1683168316832, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1790346, Length=268, Percent_Identity=30.9701492537313, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1789808, Length=290, Percent_Identity=31.0344827586207, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1788393, Length=288, Percent_Identity=28.4722222222222, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI87081816, Length=264, Percent_Identity=30.3030303030303, Blast_Score=96, Evalue=9e-21,
Organism=Escherichia coli, GI87082128, Length=293, Percent_Identity=25.938566552901, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1790551, Length=295, Percent_Identity=27.7966101694915, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786600, Length=200, Percent_Identity=29, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789149, Length=218, Percent_Identity=29.3577981651376, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1786783, Length=296, Percent_Identity=24.6621621621622, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI145693157, Length=252, Percent_Identity=25.3968253968254, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI1786912, Length=298, Percent_Identity=29.1946308724832, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI48994928, Length=220, Percent_Identity=25.9090909090909, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1788549, Length=236, Percent_Identity=26.271186440678, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790861, Length=229, Percent_Identity=27.5109170305677, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1788713, Length=270, Percent_Identity=24.0740740740741, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 63112; Mature: 62981

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLVSSLKHSIFVRIYAGLLIVCLCVALFAQLLMDTINKERVQSYRENMATGAFHIVSEG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
LAHQENEAQRDYWLSDASSLFGSEFKIVSIDEVDFNASELRRFEKENTVVRYISQLTTAD
HHCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VYYRLPDNQGVLTARISQVTEQQVRAIAVFLLDDLSYYITLSDKRARLAQLEEKFSFPLA
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHCCCHH
FKAVAALNLDPDQMARLRRDEVVILLQDTNTSKSNSSIKVIVPSEISDMAILIGPVPLFN
HHHHHHCCCCHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCEEEEECCCCCC
WFPLNLIVSMILISMFLISLGVYALIFPLERKLQLIQVGVNEVSKGNLESQVQVIGQDEI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHHH
ARLAATFNAMTSHIKRLIESQRELTRAVSHELRTPVARIRFAVDMLADTDDEDSRFMQRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHH
FIDEDIEALNGLIDEILTYAKLEEGSPKLDLEPVNLRELLEQIERETNALGKPIKIVTKL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEC
PNAKVEAIADRRYLHRVIQNLAGNALRYAETTIIISAGVKKGNAFVSVEDDGHGIPEADR
CCCHHHHHHHHHHHHHHHHHHCCCCEEHHHEEEEEECCCCCCCEEEEECCCCCCCCCCCC
EKVFIPFSRLDDSRTRASGGYGLGLSIVSRIAFWFNGGMKVDESRDLGGARFVMTWPIKP
CEEEEEHHHCCCCHHCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEEECCCHH
LTQILAADELTQDKSEREFDA
HHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SLVSSLKHSIFVRIYAGLLIVCLCVALFAQLLMDTINKERVQSYRENMATGAFHIVSEG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
LAHQENEAQRDYWLSDASSLFGSEFKIVSIDEVDFNASELRRFEKENTVVRYISQLTTAD
HHCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VYYRLPDNQGVLTARISQVTEQQVRAIAVFLLDDLSYYITLSDKRARLAQLEEKFSFPLA
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHCCCHH
FKAVAALNLDPDQMARLRRDEVVILLQDTNTSKSNSSIKVIVPSEISDMAILIGPVPLFN
HHHHHHCCCCHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCEEEEECCCCCC
WFPLNLIVSMILISMFLISLGVYALIFPLERKLQLIQVGVNEVSKGNLESQVQVIGQDEI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHHH
ARLAATFNAMTSHIKRLIESQRELTRAVSHELRTPVARIRFAVDMLADTDDEDSRFMQRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHH
FIDEDIEALNGLIDEILTYAKLEEGSPKLDLEPVNLRELLEQIERETNALGKPIKIVTKL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEC
PNAKVEAIADRRYLHRVIQNLAGNALRYAETTIIISAGVKKGNAFVSVEDDGHGIPEADR
CCCHHHHHHHHHHHHHHHHHHCCCCEEHHHEEEEEECCCCCCCEEEEECCCCCCCCCCCC
EKVFIPFSRLDDSRTRASGGYGLGLSIVSRIAFWFNGGMKVDESRDLGGARFVMTWPIKP
CEEEEEHHHCCCCHHCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEEECCCHH
LTQILAADELTQDKSEREFDA
HHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]