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The map label for this gene is trmD [H]

Identifier: 93007144

GI number: 93007144

Start: 2848869

End: 2849633

Strand: Reverse

Name: trmD [H]

Synonym: Pcryo_2320

Alternate gene names: 93007144

Gene position: 2849633-2848869 (Counterclockwise)

Preceding gene: 93007145

Following gene: 93007143

Centisome position: 93.13

GC content: 47.71

Gene sequence:

>765_bases
ATGTATTTTGCCGTTATTAGTATTTTTCCTGAAATGTTTGCCACGATTCGTGAGTTTGGAATCACAGGTCGGGCAGTCAC
TCAGAAGCAAGTAACGATTGAATGCATCAACCCGCGTGATTTTACCAGTGATAATTATCGCCGTATTGATGAACGTCCGT
ATGGTGGCGGTCCTGGCATGGTGATGATGGCTGAGCCATTATCCCAAGCGATTGAGGATGCGCGCCTACGAGCAAGTCAG
CATGGCTGCCGTGTCGACAAAGCGCATTGTCCGGTGATTTATATGTCGCCACAAGGGCAGACGCTGAGTGAGAGTAGTGT
GGTCGATATGACTGAGTATGATGGCATGATTATATTATGCGGTCGTTACGAGGGCATTGATGAGCGCTTACTGTCGCAGT
ATGTCGATATGGAAATATCACTTGGTGATTACGTGCTTACCGGTGGTGAGCTACCAGCAATGGTGCTAATGGATAGTGTG
ATACGTCGTCTGCCTGATATTATGGGTGATGATAAGTCAGCTGAGCAAGACTCGTTCGTCGATGGCTTGCTTGATTGTCC
ACATTATACTAAGCCGCATGAGTTTGCGGGTATGGCAGTGCCTGAAGTGTTACTTTCAGGTCACCATGCCAATATCGCGA
AGTGGCGCTTTAGTCAGCAAGTCGAACGTACGCAAGCGCGTCGTCCAGACTTATGGCAAGCGTTTACGCCAACGGTAGAG
CAAGCAAAGTGGCTAAAGGCATTAGCAAAAGCTGATAAAAAATAG

Upstream 100 bases:

>100_bases
CAAGCTTTAGTGGTATAAGCTTTAACTTTATCGCTTAAGATTGTTAATAGTAGTACCGATTTATTTAGCCAATATTAGCC
ACTGCGCAAGTAGACATCTG

Downstream 100 bases:

>100_bases
CGGTTAAAAAGATAAACAGTTAAGTAATAAAATTTTGAGCAATAACAAAACGAGTCATAGCATAATGGCCGTTTGTCATA
ACCTACTTACGTTGTGTCTT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MYFAVISIFPEMFATIREFGITGRAVTQKQVTIECINPRDFTSDNYRRIDERPYGGGPGMVMMAEPLSQAIEDARLRASQ
HGCRVDKAHCPVIYMSPQGQTLSESSVVDMTEYDGMIILCGRYEGIDERLLSQYVDMEISLGDYVLTGGELPAMVLMDSV
IRRLPDIMGDDKSAEQDSFVDGLLDCPHYTKPHEFAGMAVPEVLLSGHHANIAKWRFSQQVERTQARRPDLWQAFTPTVE
QAKWLKALAKADKK

Sequences:

>Translated_254_residues
MYFAVISIFPEMFATIREFGITGRAVTQKQVTIECINPRDFTSDNYRRIDERPYGGGPGMVMMAEPLSQAIEDARLRASQ
HGCRVDKAHCPVIYMSPQGQTLSESSVVDMTEYDGMIILCGRYEGIDERLLSQYVDMEISLGDYVLTGGELPAMVLMDSV
IRRLPDIMGDDKSAEQDSFVDGLLDCPHYTKPHEFAGMAVPEVLLSGHHANIAKWRFSQQVERTQARRPDLWQAFTPTVE
QAKWLKALAKADKK
>Mature_254_residues
MYFAVISIFPEMFATIREFGITGRAVTQKQVTIECINPRDFTSDNYRRIDERPYGGGPGMVMMAEPLSQAIEDARLRASQ
HGCRVDKAHCPVIYMSPQGQTLSESSVVDMTEYDGMIILCGRYEGIDERLLSQYVDMEISLGDYVLTGGELPAMVLMDSV
IRRLPDIMGDDKSAEQDSFVDGLLDCPHYTKPHEFAGMAVPEVLLSGHHANIAKWRFSQQVERTQARRPDLWQAFTPTVE
QAKWLKALAKADKK

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=245, Percent_Identity=50.6122448979592, Blast_Score=255, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 28601; Mature: 28601

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYFAVISIFPEMFATIREFGITGRAVTQKQVTIECINPRDFTSDNYRRIDERPYGGGPGM
CEEHHHHHHHHHHHHHHHHCCCCCEEECCEEEEEEECCCCCCCCCHHCCCCCCCCCCCCE
VMMAEPLSQAIEDARLRASQHGCRVDKAHCPVIYMSPQGQTLSESSVVDMTEYDGMIILC
EEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCEEHCCCCCEEEEE
GRYEGIDERLLSQYVDMEISLGDYVLTGGELPAMVLMDSVIRRLPDIMGDDKSAEQDSFV
CCCCCHHHHHHHHHHCCEEECCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
DGLLDCPHYTKPHEFAGMAVPEVLLSGHHANIAKWRFSQQVERTQARRPDLWQAFTPTVE
HHHHCCCCCCCCHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCHHH
QAKWLKALAKADKK
HHHHHHHHHHHCCC
>Mature Secondary Structure
MYFAVISIFPEMFATIREFGITGRAVTQKQVTIECINPRDFTSDNYRRIDERPYGGGPGM
CEEHHHHHHHHHHHHHHHHCCCCCEEECCEEEEEEECCCCCCCCCHHCCCCCCCCCCCCE
VMMAEPLSQAIEDARLRASQHGCRVDKAHCPVIYMSPQGQTLSESSVVDMTEYDGMIILC
EEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCEEHCCCCCEEEEE
GRYEGIDERLLSQYVDMEISLGDYVLTGGELPAMVLMDSVIRRLPDIMGDDKSAEQDSFV
CCCCCHHHHHHHHHHCCEEECCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
DGLLDCPHYTKPHEFAGMAVPEVLLSGHHANIAKWRFSQQVERTQARRPDLWQAFTPTVE
HHHHCCCCCCCCHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCHHH
QAKWLKALAKADKK
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA