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The map label for this gene is folA [H]

Identifier: 93007139

GI number: 93007139

Start: 2842834

End: 2843406

Strand: Direct

Name: folA [H]

Synonym: Pcryo_2315

Alternate gene names: 93007139

Gene position: 2842834-2843406 (Clockwise)

Preceding gene: 93007138

Following gene: 93007142

Centisome position: 92.91

GC content: 43.8

Gene sequence:

>573_bases
ATGAGTTATGCCAACACCGAAGTTGCCCAAATCGCGGCTATCAGTAGCAATCGCTGTATTGGTAAAGACAATGAGCTACC
GTGGCATATCTCAGCCGATTTGCAGCATTTTAAAAGTATGACAACCAAACCAAATGACGGTGCGATACAGGGTATCGTGA
TTATGGGTCGCAAGACCTTTGAGTCAATGGGCAGCAAGCCGTTACCTAAACGTGTGAGCTTTATTATCAGTAGCCAGATA
GATTATGCTGAGCAAAAAGGCTTGGTAGGTAGTGAAAAAGCTTATGTAATGCATAATTTAGACGATGCCTTAACCCAAGC
AGCAAGCCTAGCGCATGGTGCGCATCTTGATACGATTTGGGTAATTGGTGGCGAGCGCGTGTTTAGCGATGCATTGCTGT
ACACCGACCGTATCGAACTCACTCACGTCGATACTGAAATCGTTGATGGCGATGCCTTTTATCCTGAGCTGCCAAGTGAG
TTTGAAGTTGCGCAAGAATCTGAGCAAATGCATGATGAAAAAAGCGCGTTAGATTTTAGATTTGTGACTTATAAAAGAAA
AAACGCTGAATAA

Upstream 100 bases:

>100_bases
GGCTATGAGTCTCATCCAGCGATTAAGGCACAAGTTGCGGTATAGCGCCTGTTTATTCAATTGTATTTAAATATATTTAA
ACATTAGCAAAGGATAATTT

Downstream 100 bases:

>100_bases
TAGTAAAATTTTAAACAAAACGTAAAAAGAGGCTATCCTAAATAACTCTAGGACAGCCTCTTTTTTCATGTTCAAACTTT
ATGAACATAAACCTAAATTT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MSYANTEVAQIAAISSNRCIGKDNELPWHISADLQHFKSMTTKPNDGAIQGIVIMGRKTFESMGSKPLPKRVSFIISSQI
DYAEQKGLVGSEKAYVMHNLDDALTQAASLAHGAHLDTIWVIGGERVFSDALLYTDRIELTHVDTEIVDGDAFYPELPSE
FEVAQESEQMHDEKSALDFRFVTYKRKNAE

Sequences:

>Translated_190_residues
MSYANTEVAQIAAISSNRCIGKDNELPWHISADLQHFKSMTTKPNDGAIQGIVIMGRKTFESMGSKPLPKRVSFIISSQI
DYAEQKGLVGSEKAYVMHNLDDALTQAASLAHGAHLDTIWVIGGERVFSDALLYTDRIELTHVDTEIVDGDAFYPELPSE
FEVAQESEQMHDEKSALDFRFVTYKRKNAE
>Mature_189_residues
SYANTEVAQIAAISSNRCIGKDNELPWHISADLQHFKSMTTKPNDGAIQGIVIMGRKTFESMGSKPLPKRVSFIISSQID
YAEQKGLVGSEKAYVMHNLDDALTQAASLAHGAHLDTIWVIGGERVFSDALLYTDRIELTHVDTEIVDGDAFYPELPSEF
EVAQESEQMHDEKSALDFRFVTYKRKNAE

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=154, Percent_Identity=31.1688311688312, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI28827793, Length=152, Percent_Identity=29.6052631578947, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1786233, Length=182, Percent_Identity=32.967032967033, Blast_Score=83, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17505899, Length=185, Percent_Identity=30.2702702702703, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324810, Length=148, Percent_Identity=30.4054054054054, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI116008026, Length=164, Percent_Identity=29.8780487804878, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24647458, Length=164, Percent_Identity=29.8780487804878, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 21198; Mature: 21067

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYANTEVAQIAAISSNRCIGKDNELPWHISADLQHFKSMTTKPNDGAIQGIVIMGRKTF
CCCCCCCHHHEEEECCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCEEEEEEECCHHH
ESMGSKPLPKRVSFIISSQIDYAEQKGLVGSEKAYVMHNLDDALTQAASLAHGAHLDTIW
HHCCCCCCHHHHHHHHHCCCCHHHHCCCCCCCCEEEEECHHHHHHHHHHHHCCCCCEEEE
VIGGERVFSDALLYTDRIELTHVDTEIVDGDAFYPELPSEFEVAQESEQMHDEKSALDFR
EECCCHHHHHHHEEECCEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCEEE
FVTYKRKNAE
EEEEECCCCC
>Mature Secondary Structure 
SYANTEVAQIAAISSNRCIGKDNELPWHISADLQHFKSMTTKPNDGAIQGIVIMGRKTF
CCCCCCHHHEEEECCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCEEEEEEECCHHH
ESMGSKPLPKRVSFIISSQIDYAEQKGLVGSEKAYVMHNLDDALTQAASLAHGAHLDTIW
HHCCCCCCHHHHHHHHHCCCCHHHHCCCCCCCCEEEEECHHHHHHHHHHHHCCCCCEEEE
VIGGERVFSDALLYTDRIELTHVDTEIVDGDAFYPELPSEFEVAQESEQMHDEKSALDFR
EECCCHHHHHHHEEECCEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCEEE
FVTYKRKNAE
EEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]