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The map label for this gene is thyA

Identifier: 93007138

GI number: 93007138

Start: 2841906

End: 2842778

Strand: Direct

Name: thyA

Synonym: Pcryo_2314

Alternate gene names: 93007138

Gene position: 2841906-2842778 (Clockwise)

Preceding gene: 93007137

Following gene: 93007139

Centisome position: 92.88

GC content: 44.79

Gene sequence:

>873_bases
ATGACCATGAACAGCTCTATCATTAAAAACGAGCAAGCCTATTTAGACCTGCTACGCCTTGTCCTTAATGAAGGCACTGA
AAAAGGTGACCGTACTGGAACGGGTACGCTTAGCCATTTTGGCGCGCAGCTACGTTTTAATTTAGCCGATGGTTTTCCAC
TATTAACCACCAAAAAAGTTCACTTAAAGTCCATCACCTATGAGCTCCTATGGTTTTTAAATGGTAGTACCCACGTCGAT
TATTTACAACAAAACGGCGTACGCATCTGGAATGAATGGGCAACGAGCGAGCAAACGGCGCGCTTTAATCGTCCTGCAGG
TGACTTAGGTCCTATTTATGGTCACCAATGGCGTAATTATGGCGCGACCACAACTGCAGATGGGCAGTATAACTCTGACG
GTGTCGATCAAATCGCACAAGTGGTTGAACAAATAAAAACCAATCCTAACTCGCGGCGCTTGATTGTCTCTGGCTGGAAT
CCGGGTGAAGCAGATCAAGTTGCGCTGCCACCTTGTCATACGTTATTCCAGTTTTTTGTTGCGGATAATAAACTCTCCTG
CCAACTCTATCAGCGCTCAGCGGATTTATTTTTGGGCGTACCTTTTAATATCGCAAGCTATGCCATGCTTACCCATATGG
TGGCACAAGTTTGTAATCTAGAAGTTGGTGAATTTATTTGGACCGGTGGCGATTGCCATATTTATCAAAACCACCGTGAG
CAAGTGGAGCTGCAGCTGACCCGCTCGCTTTATACGCTGCCGACATTAGCACTTAACCCTAATGTAAAAGATATTTTTGC
TTTTAATTATGAGGATATCAGCGTTGATGGCTATGAGTCTCATCCAGCGATTAAGGCACAAGTTGCGGTATAG

Upstream 100 bases:

>100_bases
GATTGGGGTAAGCAAGCCGCTTATTAAGCTTTATCTTCTTCATGCACCACGTTAAGAACTATATTAAAGATGCGTTTTAA
TAGCGCAGAAGAGTAATTTT

Downstream 100 bases:

>100_bases
CGCCTGTTTATTCAATTGTATTTAAATATATTTAAACATTAGCAAAGGATAATTTATGAGTTATGCCAACACCGAAGTTG
CCCAAATCGCGGCTATCAGT

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MTMNSSIIKNEQAYLDLLRLVLNEGTEKGDRTGTGTLSHFGAQLRFNLADGFPLLTTKKVHLKSITYELLWFLNGSTHVD
YLQQNGVRIWNEWATSEQTARFNRPAGDLGPIYGHQWRNYGATTTADGQYNSDGVDQIAQVVEQIKTNPNSRRLIVSGWN
PGEADQVALPPCHTLFQFFVADNKLSCQLYQRSADLFLGVPFNIASYAMLTHMVAQVCNLEVGEFIWTGGDCHIYQNHRE
QVELQLTRSLYTLPTLALNPNVKDIFAFNYEDISVDGYESHPAIKAQVAV

Sequences:

>Translated_290_residues
MTMNSSIIKNEQAYLDLLRLVLNEGTEKGDRTGTGTLSHFGAQLRFNLADGFPLLTTKKVHLKSITYELLWFLNGSTHVD
YLQQNGVRIWNEWATSEQTARFNRPAGDLGPIYGHQWRNYGATTTADGQYNSDGVDQIAQVVEQIKTNPNSRRLIVSGWN
PGEADQVALPPCHTLFQFFVADNKLSCQLYQRSADLFLGVPFNIASYAMLTHMVAQVCNLEVGEFIWTGGDCHIYQNHRE
QVELQLTRSLYTLPTLALNPNVKDIFAFNYEDISVDGYESHPAIKAQVAV
>Mature_289_residues
TMNSSIIKNEQAYLDLLRLVLNEGTEKGDRTGTGTLSHFGAQLRFNLADGFPLLTTKKVHLKSITYELLWFLNGSTHVDY
LQQNGVRIWNEWATSEQTARFNRPAGDLGPIYGHQWRNYGATTTADGQYNSDGVDQIAQVVEQIKTNPNSRRLIVSGWNP
GEADQVALPPCHTLFQFFVADNKLSCQLYQRSADLFLGVPFNIASYAMLTHMVAQVCNLEVGEFIWTGGDCHIYQNHREQ
VELQLTRSLYTLPTLALNPNVKDIFAFNYEDISVDGYESHPAIKAQVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=284, Percent_Identity=49.2957746478873, Blast_Score=283, Evalue=1e-76,
Organism=Escherichia coli, GI1789191, Length=279, Percent_Identity=57.3476702508961, Blast_Score=342, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI71993377, Length=287, Percent_Identity=46.6898954703833, Blast_Score=279, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6324648, Length=303, Percent_Identity=38.9438943894389, Blast_Score=222, Evalue=5e-59,
Organism=Drosophila melanogaster, GI17137556, Length=292, Percent_Identity=47.2602739726027, Blast_Score=291, Evalue=4e-79,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_PSYCK (Q1Q8B2)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_581575.1
- ProteinModelPortal:   Q1Q8B2
- SMR:   Q1Q8B2
- STRING:   Q1Q8B2
- GeneID:   4035331
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_2314
- NMPDR:   fig|335284.3.peg.2383
- eggNOG:   COG0207
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- PhylomeDB:   Q1Q8B2
- ProtClustDB:   PRK01827
- BioCyc:   PCRY335284:PCRYO_2314-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 32621; Mature: 32490

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 172-172

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMNSSIIKNEQAYLDLLRLVLNEGTEKGDRTGTGTLSHFGAQLRFNLADGFPLLTTKKV
CCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCEEEEEECCCCCEEEECCH
HLKSITYELLWFLNGSTHVDYLQQNGVRIWNEWATSEQTARFNRPAGDLGPIYGHQWRNY
HHHHHHEEEEEEECCCCHHHHHHHCCCEEEEHHCCCCHHHHCCCCCCCCCCCCCCCHHCC
GATTTADGQYNSDGVDQIAQVVEQIKTNPNSRRLIVSGWNPGEADQVALPPCHTLFQFFV
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEECCCHHHHHHHHH
ADNKLSCQLYQRSADLFLGVPFNIASYAMLTHMVAQVCNLEVGEFIWTGGDCHIYQNHRE
CCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHCCCHHHEEEECCEEEEECCCCH
QVELQLTRSLYTLPTLALNPNVKDIFAFNYEDISVDGYESHPAIKAQVAV
HHHHHHHHHHHHCCEEEECCCCHHEEEECCCEEEECCCCCCCCEEEEECC
>Mature Secondary Structure 
TMNSSIIKNEQAYLDLLRLVLNEGTEKGDRTGTGTLSHFGAQLRFNLADGFPLLTTKKV
CCCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCEEEEEECCCCCEEEECCH
HLKSITYELLWFLNGSTHVDYLQQNGVRIWNEWATSEQTARFNRPAGDLGPIYGHQWRNY
HHHHHHEEEEEEECCCCHHHHHHHCCCEEEEHHCCCCHHHHCCCCCCCCCCCCCCCHHCC
GATTTADGQYNSDGVDQIAQVVEQIKTNPNSRRLIVSGWNPGEADQVALPPCHTLFQFFV
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEECCCHHHHHHHHH
ADNKLSCQLYQRSADLFLGVPFNIASYAMLTHMVAQVCNLEVGEFIWTGGDCHIYQNHRE
CCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHCCCHHHEEEECCEEEEECCCCH
QVELQLTRSLYTLPTLALNPNVKDIFAFNYEDISVDGYESHPAIKAQVAV
HHHHHHHHHHHHCCEEEECCCCHHEEEECCCEEEECCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA