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The map label for this gene is dusC [H]

Identifier: 93007131

GI number: 93007131

Start: 2835067

End: 2836089

Strand: Direct

Name: dusC [H]

Synonym: Pcryo_2307

Alternate gene names: 93007131

Gene position: 2835067-2836089 (Clockwise)

Preceding gene: 93007130

Following gene: 93007134

Centisome position: 92.65

GC content: 45.26

Gene sequence:

>1023_bases
ATGAATAATAGTCAGCAACTTCAAATACGTCAGAAAACAATACAAAATCTATTTGCCAATCCTGTCCGACTCCTTGCACC
AATGGAAGGTTTGACCGACCCGTTAATGCGGCAAATCCTCACGCAAATTGCATCAGACTTGGGTCGCCCTTATGATTGGT
CGGTTAGCGAATTTATCCGTGTGACTCAGCATGTCTTACCCGCGCATGTATTCTATAAATACGTTCCAGAATTGCGTCAT
GATGCCAAGACTGCCTGTGGCACACCGATTCATATCCAGTTGCTCGGTAGTGAAGCGCAACTCATGGCAGACAATGCGGC
ATATGCCTGTGCGCTTGGTGCACCTGCCATTGATATCAACTTTGGTTGTCCTGCCAAAACTGTCAATAGCCACCGTGGTG
GTTCGGTATTACTGGATGAACCCGAAACCATGTATCAGATTATCAGCGCGGTGCGTCAAGCGGTACCTGCCGACATTCCA
GTCTCAGCCAAAATTCGTTTAGGTTATACCGATACCAGTCTCATGGATGATATTCGCGGCGCGATTAAAGACAGTGGTGC
TGATTGGCTAACTGTTCATGCACGAACCAAAACCCAAGGCTATAAGCCGCCAGCCTATTGGGACAAGATTCAAAGCTTTA
ATGCGCTTGATATTCCCGTCATTGCCAATGGCGAAATATGGAATGCAGAGCACGCACAAAATTGTATGACGCAATCGGGT
ACAGAGCATTTGATGTTGGGTCGCGGTGCAGTTACCCGTCCCGACTTAATTGCACAGGTTGATAGAGAACATAGTCAGAC
TTTAAATAATGAAACAACATTGTTATGGCAAGACCTGATTGCGCATCAGATTAAATTTTTGCAAGGCACGGCAAAAAGCG
ACATTGTATTGGTCGGTCGTTATAAACAATGGTTAGCGATGCTCACCAAAGGCTATAGCGAGGCGAAGATATTATGGGAC
AGTATTAAAAGAGAGAAAAATAAAGCAGCTATCATTAGCGCACTACAAATGAGTGCACAGTAA

Upstream 100 bases:

>100_bases
CCTAACTTAATCAATCTTTGTATTTGCCACTCTAAACCTCTAGCCTATCTTTCTTTATGGCAACCTTTTAATAGCACCCT
TTTAGCAGTGAACAACAAGC

Downstream 100 bases:

>100_bases
CGTTAGCTGCTTTTATCTTATAAGCATTAGTTTTTTCAATCAGCTGCTACTATACACTGACTCGAATAGCTAAATAAAAG
AGCATGAGACAGCAGGCAAT

Product: dihydrouridine synthase, DuS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MNNSQQLQIRQKTIQNLFANPVRLLAPMEGLTDPLMRQILTQIASDLGRPYDWSVSEFIRVTQHVLPAHVFYKYVPELRH
DAKTACGTPIHIQLLGSEAQLMADNAAYACALGAPAIDINFGCPAKTVNSHRGGSVLLDEPETMYQIISAVRQAVPADIP
VSAKIRLGYTDTSLMDDIRGAIKDSGADWLTVHARTKTQGYKPPAYWDKIQSFNALDIPVIANGEIWNAEHAQNCMTQSG
TEHLMLGRGAVTRPDLIAQVDREHSQTLNNETTLLWQDLIAHQIKFLQGTAKSDIVLVGRYKQWLAMLTKGYSEAKILWD
SIKREKNKAAIISALQMSAQ

Sequences:

>Translated_340_residues
MNNSQQLQIRQKTIQNLFANPVRLLAPMEGLTDPLMRQILTQIASDLGRPYDWSVSEFIRVTQHVLPAHVFYKYVPELRH
DAKTACGTPIHIQLLGSEAQLMADNAAYACALGAPAIDINFGCPAKTVNSHRGGSVLLDEPETMYQIISAVRQAVPADIP
VSAKIRLGYTDTSLMDDIRGAIKDSGADWLTVHARTKTQGYKPPAYWDKIQSFNALDIPVIANGEIWNAEHAQNCMTQSG
TEHLMLGRGAVTRPDLIAQVDREHSQTLNNETTLLWQDLIAHQIKFLQGTAKSDIVLVGRYKQWLAMLTKGYSEAKILWD
SIKREKNKAAIISALQMSAQ
>Mature_340_residues
MNNSQQLQIRQKTIQNLFANPVRLLAPMEGLTDPLMRQILTQIASDLGRPYDWSVSEFIRVTQHVLPAHVFYKYVPELRH
DAKTACGTPIHIQLLGSEAQLMADNAAYACALGAPAIDINFGCPAKTVNSHRGGSVLLDEPETMYQIISAVRQAVPADIP
VSAKIRLGYTDTSLMDDIRGAIKDSGADWLTVHARTKTQGYKPPAYWDKIQSFNALDIPVIANGEIWNAEHAQNCMTQSG
TEHLMLGRGAVTRPDLIAQVDREHSQTLNNETTLLWQDLIAHQIKFLQGTAKSDIVLVGRYKQWLAMLTKGYSEAKILWD
SIKREKNKAAIISALQMSAQ

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=173, Percent_Identity=32.3699421965318, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI239788483, Length=202, Percent_Identity=27.7227722772277, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI239788462, Length=202, Percent_Identity=27.7227722772277, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI40807366, Length=147, Percent_Identity=30.6122448979592, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788462, Length=317, Percent_Identity=41.9558359621451, Blast_Score=239, Evalue=1e-64,
Organism=Escherichia coli, GI1789660, Length=233, Percent_Identity=33.9055793991416, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI145693211, Length=190, Percent_Identity=28.4210526315789, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI25144369, Length=209, Percent_Identity=30.622009569378, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17543114, Length=228, Percent_Identity=25.8771929824561, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=185, Percent_Identity=30.2702702702703, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323437, Length=254, Percent_Identity=24.4094488188976, Blast_Score=66, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=180, Percent_Identity=26.6666666666667, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24580595, Length=194, Percent_Identity=33.5051546391753, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19920448, Length=194, Percent_Identity=33.5051546391753, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19921524, Length=149, Percent_Identity=32.2147651006711, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24585320, Length=153, Percent_Identity=28.1045751633987, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37784; Mature: 37784

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNSQQLQIRQKTIQNLFANPVRLLAPMEGLTDPLMRQILTQIASDLGRPYDWSVSEFIR
CCCCHHHHHHHHHHHHHHCCHHHHHHCHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
VTQHVLPAHVFYKYVPELRHDAKTACGTPIHIQLLGSEAQLMADNAAYACALGAPAIDIN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHEEECCCCEEEEECCCEEEEE
FGCPAKTVNSHRGGSVLLDEPETMYQIISAVRQAVPADIPVSAKIRLGYTDTSLMDDIRG
CCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHH
AIKDSGADWLTVHARTKTQGYKPPAYWDKIQSFNALDIPVIANGEIWNAEHAQNCMTQSG
HHHCCCCCEEEEEEEECCCCCCCCHHHHHHCCCCCCCCCEEECCCEECHHHHHHHHHCCC
TEHLMLGRGAVTRPDLIAQVDREHSQTLNNETTLLWQDLIAHQIKFLQGTAKSDIVLVGR
CCEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
YKQWLAMLTKGYSEAKILWDSIKREKNKAAIISALQMSAQ
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNSQQLQIRQKTIQNLFANPVRLLAPMEGLTDPLMRQILTQIASDLGRPYDWSVSEFIR
CCCCHHHHHHHHHHHHHHCCHHHHHHCHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
VTQHVLPAHVFYKYVPELRHDAKTACGTPIHIQLLGSEAQLMADNAAYACALGAPAIDIN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHEEECCCCEEEEECCCEEEEE
FGCPAKTVNSHRGGSVLLDEPETMYQIISAVRQAVPADIPVSAKIRLGYTDTSLMDDIRG
CCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHH
AIKDSGADWLTVHARTKTQGYKPPAYWDKIQSFNALDIPVIANGEIWNAEHAQNCMTQSG
HHHCCCCCEEEEEEEECCCCCCCCHHHHHHCCCCCCCCCEEECCCEECHHHHHHHHHCCC
TEHLMLGRGAVTRPDLIAQVDREHSQTLNNETTLLWQDLIAHQIKFLQGTAKSDIVLVGR
CCEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
YKQWLAMLTKGYSEAKILWDSIKREKNKAAIISALQMSAQ
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]