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The map label for this gene is tyrS [H]

Identifier: 93007093

GI number: 93007093

Start: 2785839

End: 2787050

Strand: Reverse

Name: tyrS [H]

Synonym: Pcryo_2269

Alternate gene names: 93007093

Gene position: 2787050-2785839 (Counterclockwise)

Preceding gene: 93007097

Following gene: 93007092

Centisome position: 91.08

GC content: 44.39

Gene sequence:

>1212_bases
ATGACCACCCAAACTTATACCCTAGAAGAACAACTAGCGATAATCCAACGCGGTACACAAGAAATCTTATCTGAAGATGA
TTTGGTTAAGAAGCTAAAACTCAATCGTCCGCTGCGTATCAAAGCAGGTTTTGATCCTACCGCGCCAGACTTACATTTGG
GTCATACCGTATTGATTAATAAACTGAAGCATTTTCAAGATTTGGGTCATGAGATTTATTTTCTAATCGGTGATTATACC
GCCAAGATTGGTGATCCTTCTGGCAAAAACAGCACGCGTCCGCCATTGACCGATGAGCAAATCAAAGTCAACGCTCAGAC
TTATGCTGAGCAAGTCTTTAAGATTTTGGATAAAGAAAAAACCCGCATCGTCTTTAACTCTGAATGGTTTAAAGACATGT
CAGCAGGCGATATGATTCAGCTTTCTAGCCAATTGACCGTCTCACGTATGCTTGAGCGCGATGATTTCTCTAAACGCTAC
GCGGCGCAAACGCCAATTGCTATCCATGAGTTTCTCTACCCATTGGTGCAAGGTTATGACTCTATCGCACTAAAAGCCGA
CGTTGAGCTTGGCGGTACTGACCAAACCTTTAACATCCTAATGGGACGCACCTTGCAAGGTCGTTACGGTCAAGAGCCAC
AAGTTTGTATCACGGTGCCAATCTTAGAAGGGCTAGACGGCGTTAATAAAATGTCGAAGTCACTTGGTAACTATGTCGGC
ATTTATGATGCTCCGGGTACCATGTACCAAAAACTTCTATCGATGCCAGATACCTTAATCCGTCGCTACTTTGAGTTTTT
AAGCTTTAAGCCGATGGAAGAAGTCGAAGCGTTAATGGCAGAGATGGAAAACGGTCGCAATCCACAAGAAATCAAACGCA
TTCTTGCCGAAGAATTGATCGAGCGCTTCCATGATGCCGATGCTGCTGCTAATGCGCATAAATCAGCGGGTAACGTGCTT
GCTGATGGCGAATTGCCAGTCGATTTACCAGAAGTGACCTTAGATTTAGAAGGGCAGGACGCATTATTTATCACGCAGAT
ATTAAACCAAGCAGAGCTTGCCAAAAATAGCTCATCGGCTAAAGATATGATTAAACGCGGCGCGGTGAAAGTAGATGGCG
AAGTCGTCGACGCGGGCTTTAGCTTAACCTCTGGTCAGACAGTCGTGATTCAAGCAGGCAAAAAGGCTTATGCTAAAGTG
ACAGTAGCTTAA

Upstream 100 bases:

>100_bases
CGCTAGTATATCTGTCAAATTCTAACATTTTTTGATGGCTACGAGATTCTAAATAGCCCAAACGCTTTACCCTTTAAATA
AAGACAATAAAGAGAGCATC

Downstream 100 bases:

>100_bases
TAATTTATTGAGATAAGCTATTGAGAGTAAGAATAAGGTCGACATGATGGTCGACCTTATCTATTTTTGCGATGTAAGTA
TAATGAGAAAGTATTTGATT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MTTQTYTLEEQLAIIQRGTQEILSEDDLVKKLKLNRPLRIKAGFDPTAPDLHLGHTVLINKLKHFQDLGHEIYFLIGDYT
AKIGDPSGKNSTRPPLTDEQIKVNAQTYAEQVFKILDKEKTRIVFNSEWFKDMSAGDMIQLSSQLTVSRMLERDDFSKRY
AAQTPIAIHEFLYPLVQGYDSIALKADVELGGTDQTFNILMGRTLQGRYGQEPQVCITVPILEGLDGVNKMSKSLGNYVG
IYDAPGTMYQKLLSMPDTLIRRYFEFLSFKPMEEVEALMAEMENGRNPQEIKRILAEELIERFHDADAAANAHKSAGNVL
ADGELPVDLPEVTLDLEGQDALFITQILNQAELAKNSSSAKDMIKRGAVKVDGEVVDAGFSLTSGQTVVIQAGKKAYAKV
TVA

Sequences:

>Translated_403_residues
MTTQTYTLEEQLAIIQRGTQEILSEDDLVKKLKLNRPLRIKAGFDPTAPDLHLGHTVLINKLKHFQDLGHEIYFLIGDYT
AKIGDPSGKNSTRPPLTDEQIKVNAQTYAEQVFKILDKEKTRIVFNSEWFKDMSAGDMIQLSSQLTVSRMLERDDFSKRY
AAQTPIAIHEFLYPLVQGYDSIALKADVELGGTDQTFNILMGRTLQGRYGQEPQVCITVPILEGLDGVNKMSKSLGNYVG
IYDAPGTMYQKLLSMPDTLIRRYFEFLSFKPMEEVEALMAEMENGRNPQEIKRILAEELIERFHDADAAANAHKSAGNVL
ADGELPVDLPEVTLDLEGQDALFITQILNQAELAKNSSSAKDMIKRGAVKVDGEVVDAGFSLTSGQTVVIQAGKKAYAKV
TVA
>Mature_402_residues
TTQTYTLEEQLAIIQRGTQEILSEDDLVKKLKLNRPLRIKAGFDPTAPDLHLGHTVLINKLKHFQDLGHEIYFLIGDYTA
KIGDPSGKNSTRPPLTDEQIKVNAQTYAEQVFKILDKEKTRIVFNSEWFKDMSAGDMIQLSSQLTVSRMLERDDFSKRYA
AQTPIAIHEFLYPLVQGYDSIALKADVELGGTDQTFNILMGRTLQGRYGQEPQVCITVPILEGLDGVNKMSKSLGNYVGI
YDAPGTMYQKLLSMPDTLIRRYFEFLSFKPMEEVEALMAEMENGRNPQEIKRILAEELIERFHDADAAANAHKSAGNVLA
DGELPVDLPEVTLDLEGQDALFITQILNQAELAKNSSSAKDMIKRGAVKVDGEVVDAGFSLTSGQTVVIQAGKKAYAKVT
VA

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=320, Percent_Identity=30.3125, Blast_Score=115, Evalue=6e-26,
Organism=Escherichia coli, GI1787925, Length=415, Percent_Identity=28.6746987951807, Blast_Score=140, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI25145802, Length=396, Percent_Identity=26.5151515151515, Blast_Score=108, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6325160, Length=419, Percent_Identity=24.582338902148, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI20130383, Length=402, Percent_Identity=26.6169154228856, Blast_Score=142, Evalue=6e-34,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 44837; Mature: 44706

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQTYTLEEQLAIIQRGTQEILSEDDLVKKLKLNRPLRIKAGFDPTAPDLHLGHTVLIN
CCCCEECHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHH
KLKHFQDLGHEIYFLIGDYTAKIGDPSGKNSTRPPLTDEQIKVNAQTYAEQVFKILDKEK
HHHHHHHCCCEEEEEEECEEEECCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHCCCC
TRIVFNSEWFKDMSAGDMIQLSSQLTVSRMLERDDFSKRYAAQTPIAIHEFLYPLVQGYD
CEEEECCHHHHCCCCCCEEHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHCCCC
SIALKADVELGGTDQTFNILMGRTLQGRYGQEPQVCITVPILEGLDGVNKMSKSLGNYVG
CEEEEEEEEECCCCCHHHHHHCCCCCCCCCCCCCEEEEEEHHHCCCHHHHHHHHHCCEEE
IYDAPGTMYQKLLSMPDTLIRRYFEFLSFKPMEEVEALMAEMENGRNPQEIKRILAEELI
EECCCHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ERFHDADAAANAHKSAGNVLADGELPVDLPEVTLDLEGQDALFITQILNQAELAKNSSSA
HHHHCCHHHHHCHHCCCCEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHH
KDMIKRGAVKVDGEVVDAGFSLTSGQTVVIQAGKKAYAKVTVA
HHHHHCCCEEECCEEEECCEEECCCCEEEEECCCCEEEEEEEC
>Mature Secondary Structure 
TTQTYTLEEQLAIIQRGTQEILSEDDLVKKLKLNRPLRIKAGFDPTAPDLHLGHTVLIN
CCCEECHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHH
KLKHFQDLGHEIYFLIGDYTAKIGDPSGKNSTRPPLTDEQIKVNAQTYAEQVFKILDKEK
HHHHHHHCCCEEEEEEECEEEECCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHCCCC
TRIVFNSEWFKDMSAGDMIQLSSQLTVSRMLERDDFSKRYAAQTPIAIHEFLYPLVQGYD
CEEEECCHHHHCCCCCCEEHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHCCCC
SIALKADVELGGTDQTFNILMGRTLQGRYGQEPQVCITVPILEGLDGVNKMSKSLGNYVG
CEEEEEEEEECCCCCHHHHHHCCCCCCCCCCCCCEEEEEEHHHCCCHHHHHHHHHCCEEE
IYDAPGTMYQKLLSMPDTLIRRYFEFLSFKPMEEVEALMAEMENGRNPQEIKRILAEELI
EECCCHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ERFHDADAAANAHKSAGNVLADGELPVDLPEVTLDLEGQDALFITQILNQAELAKNSSSA
HHHHCCHHHHHCHHCCCCEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHH
KDMIKRGAVKVDGEVVDAGFSLTSGQTVVIQAGKKAYAKVTVA
HHHHHCCCEEECCEEEECCEEECCCCEEEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA