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The map label for this gene is siaT [H]

Identifier: 93007083

GI number: 93007083

Start: 2770787

End: 2772076

Strand: Reverse

Name: siaT [H]

Synonym: Pcryo_2259

Alternate gene names: 93007083

Gene position: 2772076-2770787 (Counterclockwise)

Preceding gene: 93007084

Following gene: 93007082

Centisome position: 90.59

GC content: 45.04

Gene sequence:

>1290_bases
ATGAGTCCAGAAATTATTGGTGCCATTGGTTTGTTATGTATGATTGCGATGGTCATGCTGCGTGTTCCTGTTGCACTTGC
GATGCTTGGTGTTGGTTTGGTTGGCTTTGGTGCCGTGACAGCACCGATTGGTGCGGTGCAGATGCTCAAAGACTTACCCG
TTGATGTTTTAGCGAAATATGATTTTAGTGCTATTCCATTGTTCATACTTATGGGTGTCTTTGCTTCTCATTCTGGTATG
GCAGGGAAGTTATTTGATGCTACTCGAACTATCTTTGGTGGCGTTAGGGGTAGTCTAGGTATTGCTGGTATTGGTTCATC
GGGTATCTTTGCATCCATTTCAGGGTCATCATTGGCGACAGCGTCTACCATGACCAAAGTAGCATTGCCACAAATGGAGA
AGTACGGTTATCAGCCAGGCTTTGCCTGCGGTATTTTAGCCGCTGGTGGTACTTTGGGAATCATGATTCCGCCCAGTATT
GCACTGCTGGTTTATGCCATCCTAACCCAGCAGTCAGTTGGTGATATGTTTATCGCTGGCTTCTTACCGGGTATGCTCGG
CATGGTGATGTACTCAATCACTGTTATGATCATGGTACGCTGGAAGCCACATTTAGCACAACGTGGGGAGCCTACTTCTT
GGAAACAAAAGCTAATCTCTTTGACAGGTCTTATTCCCTTTAGCTTTATCTTCATCGTGATTATCGCGGGTATTTTCTTT
GGGCTATTTACCCCAACAGAAGGCGCTGCAGTTGGGGCGTTTGTCTCTTGGGCTTATGCTTTTGCCAAAGGCATGCGAAT
GATGGGGCTTAAGCAATCATTAATTGAGACGCTTGCCCTCTCTGCGGTGGTCTTCTTTATGTTACTTGGTGCTGAAGCAC
TCGGCTACTTTATCTCAGTATCGCGCTTGTCTTATAGTTTGGCGACGTGGATTGGTACATTGGCAGTTAGCCCAATGATA
GTATTGATCTGCATTTTGATTATGTACTTTCTGTTAGGCTTGTTCATGGATGCCCTAGCGATGCTGGTGATTACGATTCC
GGTAGTGTTCCCAATCATTGTTGCGTTAGGTTATGATCCGGTTTGGTTTGGTATTATTGCAGTATTAACCGTAGAGCTCG
GACTCATTACACCGCCTATGGGTATGAATATCTTTGTGATTAAGGCAATGGCACCACATATTAAGCTGTCCGATATGTTC
AAGGGGGTGATGCCTTTTATTGTCTCTGACTTGATTCGCTTAGTCATTTTGGTCGCGTTCCCAGCGATATCTTTAGTGCT
ACTAAGTTAA

Upstream 100 bases:

>100_bases
TTAGTGCCTTCTTAAGCGTTTTACTTGCCATTCGCTCATTGATCGAATCCATCAACATCTTTAAAACAGGCGAGTTCTTT
GACGCTGAGGAGACAGGCGC

Downstream 100 bases:

>100_bases
TTCCTACATAAAATAACACTGGAGCATTGTTGCGTACTATGTATAACGCTAGAATAATTGAGAGACATCGTTTTGAAGTA
GCATCTCTACGATAAGCTTG

Product: TRAP dicarboxylate transporter- DctM subunit

Products: NA

Alternate protein names: N-acetylneuraminic acid permease; N-acetylneuraminic acid transporter; Neu5Ac permease [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSPEIIGAIGLLCMIAMVMLRVPVALAMLGVGLVGFGAVTAPIGAVQMLKDLPVDVLAKYDFSAIPLFILMGVFASHSGM
AGKLFDATRTIFGGVRGSLGIAGIGSSGIFASISGSSLATASTMTKVALPQMEKYGYQPGFACGILAAGGTLGIMIPPSI
ALLVYAILTQQSVGDMFIAGFLPGMLGMVMYSITVMIMVRWKPHLAQRGEPTSWKQKLISLTGLIPFSFIFIVIIAGIFF
GLFTPTEGAAVGAFVSWAYAFAKGMRMMGLKQSLIETLALSAVVFFMLLGAEALGYFISVSRLSYSLATWIGTLAVSPMI
VLICILIMYFLLGLFMDALAMLVITIPVVFPIIVALGYDPVWFGIIAVLTVELGLITPPMGMNIFVIKAMAPHIKLSDMF
KGVMPFIVSDLIRLVILVAFPAISLVLLS

Sequences:

>Translated_429_residues
MSPEIIGAIGLLCMIAMVMLRVPVALAMLGVGLVGFGAVTAPIGAVQMLKDLPVDVLAKYDFSAIPLFILMGVFASHSGM
AGKLFDATRTIFGGVRGSLGIAGIGSSGIFASISGSSLATASTMTKVALPQMEKYGYQPGFACGILAAGGTLGIMIPPSI
ALLVYAILTQQSVGDMFIAGFLPGMLGMVMYSITVMIMVRWKPHLAQRGEPTSWKQKLISLTGLIPFSFIFIVIIAGIFF
GLFTPTEGAAVGAFVSWAYAFAKGMRMMGLKQSLIETLALSAVVFFMLLGAEALGYFISVSRLSYSLATWIGTLAVSPMI
VLICILIMYFLLGLFMDALAMLVITIPVVFPIIVALGYDPVWFGIIAVLTVELGLITPPMGMNIFVIKAMAPHIKLSDMF
KGVMPFIVSDLIRLVILVAFPAISLVLLS
>Mature_428_residues
SPEIIGAIGLLCMIAMVMLRVPVALAMLGVGLVGFGAVTAPIGAVQMLKDLPVDVLAKYDFSAIPLFILMGVFASHSGMA
GKLFDATRTIFGGVRGSLGIAGIGSSGIFASISGSSLATASTMTKVALPQMEKYGYQPGFACGILAAGGTLGIMIPPSIA
LLVYAILTQQSVGDMFIAGFLPGMLGMVMYSITVMIMVRWKPHLAQRGEPTSWKQKLISLTGLIPFSFIFIVIIAGIFFG
LFTPTEGAAVGAFVSWAYAFAKGMRMMGLKQSLIETLALSAVVFFMLLGAEALGYFISVSRLSYSLATWIGTLAVSPMIV
LICILIMYFLLGLFMDALAMLVITIPVVFPIIVALGYDPVWFGIIAVLTVELGLITPPMGMNIFVIKAMAPHIKLSDMFK
GVMPFIVSDLIRLVILVAFPAISLVLLS

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system siaPT involved in the uptake of sialic acid [H]

COG id: COG1593

COG function: function code G; TRAP-type C4-dicarboxylate transport system, large permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the TRAP transporter large permease family [H]

Homologues:

Organism=Escherichia coli, GI48994950, Length=364, Percent_Identity=29.6703296703297, Blast_Score=141, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010656
- InterPro:   IPR007387
- InterPro:   IPR004681 [H]

Pfam domain/function: PF06808 DctM; PF04290 DctQ [H]

EC number: NA

Molecular weight: Translated: 45456; Mature: 45325

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
7.0 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
6.8 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPEIIGAIGLLCMIAMVMLRVPVALAMLGVGLVGFGAVTAPIGAVQMLKDLPVDVLAKY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHC
DFSAIPLFILMGVFASHSGMAGKLFDATRTIFGGVRGSLGIAGIGSSGIFASISGSSLAT
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEECCCCHHH
ASTMTKVALPQMEKYGYQPGFACGILAAGGTLGIMIPPSIALLVYAILTQQSVGDMFIAG
HHHHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
FLPGMLGMVMYSITVMIMVRWKPHLAQRGEPTSWKQKLISLTGLIPFSFIFIVIIAGIFF
HHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLFTPTEGAAVGAFVSWAYAFAKGMRMMGLKQSLIETLALSAVVFFMLLGAEALGYFISV
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRLSYSLATWIGTLAVSPMIVLICILIMYFLLGLFMDALAMLVITIPVVFPIIVALGYDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VWFGIIAVLTVELGLITPPMGMNIFVIKAMAPHIKLSDMFKGVMPFIVSDLIRLVILVAF
HHHHHHHHHHHHHHHCCCCCCCCEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
PAISLVLLS
HHHHHHHCC
>Mature Secondary Structure 
SPEIIGAIGLLCMIAMVMLRVPVALAMLGVGLVGFGAVTAPIGAVQMLKDLPVDVLAKY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHC
DFSAIPLFILMGVFASHSGMAGKLFDATRTIFGGVRGSLGIAGIGSSGIFASISGSSLAT
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEECCCCHHH
ASTMTKVALPQMEKYGYQPGFACGILAAGGTLGIMIPPSIALLVYAILTQQSVGDMFIAG
HHHHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
FLPGMLGMVMYSITVMIMVRWKPHLAQRGEPTSWKQKLISLTGLIPFSFIFIVIIAGIFF
HHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLFTPTEGAAVGAFVSWAYAFAKGMRMMGLKQSLIETLALSAVVFFMLLGAEALGYFISV
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRLSYSLATWIGTLAVSPMIVLICILIMYFLLGLFMDALAMLVITIPVVFPIIVALGYDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VWFGIIAVLTVELGLITPPMGMNIFVIKAMAPHIKLSDMFKGVMPFIVSDLIRLVILVAF
HHHHHHHHHHHHHHHCCCCCCCCEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
PAISLVLLS
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]