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The map label for this gene is fadA [H]

Identifier: 93007050

GI number: 93007050

Start: 2730676

End: 2731848

Strand: Reverse

Name: fadA [H]

Synonym: Pcryo_2226

Alternate gene names: 93007050

Gene position: 2731848-2730676 (Counterclockwise)

Preceding gene: 93007051

Following gene: 93007049

Centisome position: 89.28

GC content: 48.42

Gene sequence:

>1173_bases
ATGACAATTTTAAGTCCAAAAGACGTGGTCATCGTAGATGGCGTACGCTCAGCGATGGGTAAAACTAAAAACGGTATGTT
CCGCCACGTTCGCGCTGATAGCATGTCTGCTGAATTGGTACGTGCATTGGTTGAGCGTAACGACTTTGACCCACGTGACG
TCGAAGACATCATCTGGGGTTGTGTCAACCAAACGCTAGAGCAAGGTCTAAACATCGGTCGTAACATCGGTCTGCTAGCG
GGTATTCCAAAGACTGCTGGCGGTCAAACCATTAACCGTCTATGTGGTTCATCTATGCAGGCGCTACACACTGCTGCTGC
TCAAATCATGACTGGTCAAGGTGATGTTTTCATCATCGGTGGTGTAGAGCACATGGGTCACGTCGGCATGATGCATGGCG
TTGATCTGAATCCTGAAGCGTCAAAGCATTATGCAAAAGCCTCAAACATGATGGGTTTGACCGCTGAAATGCTTGGCCGT
ATGAACAACATCACCCGCGAAGAACAAGATGCCTTTGGTCTTGAGTCGCATCGCCGTGCATGGGCTGCTACCACTGAAGG
TCGTTTTGACAATGAAATCATCGGTATCGAAGGTCATGACGAAGCAGGTCGCTTGCAACTATGTACTGTCGATGAAGTGA
TTCGTCCTGATGCGACGATGGAGCAAATGCAAAAGCTACGTCCAGCCTTTGATCCAGTAGGCGGTACGGTGACTGCTGCT
ACCTCATCTGCATTATCTGATGGTGCGTCAGCGATGCTGATCATGAGTGCGCAAAAAGCTAAAGAACTAGGTCTAAAGCC
ACGTGCTCGCATTCGTAGCATGGCAATTGCTGGTTGTGATGCCGCTATCATGGGCTACGGTCCAGTACCTGCGACGCAAA
AAGCACTTAAGCGTGCTGGCATGAGCATCGATGATATGCAAACCATCGAGCTAAACGAAGCGTTCGCAGCGCAAGGCTTA
TCAGTATTAAAAGCATTGAACTTGACTGATAAGCAAGATATCGTCAACATCAATGGCGGCGCGATTGCTTTAGGTCATCC
ACTAGGTTGTTCTGGCGCTCGTATCACAGTTACCTTGCTAAATGCTATGGAACAATCAGATACTGAAATCGGTCTTGCGA
CCATGTGTATCGGTCTTGGCCAAGGTATCGCCACTATTATTGAGCGTGTTTAA

Upstream 100 bases:

>100_bases
AAGATGATTGGTAATTTATTTATTATCAATAACCTTCTAAATTAGATAGGCGAGCATTACGGTACTGCCACTGACAATAA
AAATTGAAAAGGAAGATAGT

Downstream 100 bases:

>100_bases
GCCTATAGTATTGCAATAATTTTAAAATAGTTAAGCGCTCTAATATGAACCCCTAGTTATCATAACTGGGGGTTTTTGTA
TTTATGAATCATAGTGGTTT

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MTILSPKDVVIVDGVRSAMGKTKNGMFRHVRADSMSAELVRALVERNDFDPRDVEDIIWGCVNQTLEQGLNIGRNIGLLA
GIPKTAGGQTINRLCGSSMQALHTAAAQIMTGQGDVFIIGGVEHMGHVGMMHGVDLNPEASKHYAKASNMMGLTAEMLGR
MNNITREEQDAFGLESHRRAWAATTEGRFDNEIIGIEGHDEAGRLQLCTVDEVIRPDATMEQMQKLRPAFDPVGGTVTAA
TSSALSDGASAMLIMSAQKAKELGLKPRARIRSMAIAGCDAAIMGYGPVPATQKALKRAGMSIDDMQTIELNEAFAAQGL
SVLKALNLTDKQDIVNINGGAIALGHPLGCSGARITVTLLNAMEQSDTEIGLATMCIGLGQGIATIIERV

Sequences:

>Translated_390_residues
MTILSPKDVVIVDGVRSAMGKTKNGMFRHVRADSMSAELVRALVERNDFDPRDVEDIIWGCVNQTLEQGLNIGRNIGLLA
GIPKTAGGQTINRLCGSSMQALHTAAAQIMTGQGDVFIIGGVEHMGHVGMMHGVDLNPEASKHYAKASNMMGLTAEMLGR
MNNITREEQDAFGLESHRRAWAATTEGRFDNEIIGIEGHDEAGRLQLCTVDEVIRPDATMEQMQKLRPAFDPVGGTVTAA
TSSALSDGASAMLIMSAQKAKELGLKPRARIRSMAIAGCDAAIMGYGPVPATQKALKRAGMSIDDMQTIELNEAFAAQGL
SVLKALNLTDKQDIVNINGGAIALGHPLGCSGARITVTLLNAMEQSDTEIGLATMCIGLGQGIATIIERV
>Mature_389_residues
TILSPKDVVIVDGVRSAMGKTKNGMFRHVRADSMSAELVRALVERNDFDPRDVEDIIWGCVNQTLEQGLNIGRNIGLLAG
IPKTAGGQTINRLCGSSMQALHTAAAQIMTGQGDVFIIGGVEHMGHVGMMHGVDLNPEASKHYAKASNMMGLTAEMLGRM
NNITREEQDAFGLESHRRAWAATTEGRFDNEIIGIEGHDEAGRLQLCTVDEVIRPDATMEQMQKLRPAFDPVGGTVTAAT
SSALSDGASAMLIMSAQKAKELGLKPRARIRSMAIAGCDAAIMGYGPVPATQKALKRAGMSIDDMQTIELNEAFAAQGLS
VLKALNLTDKQDIVNINGGAIALGHPLGCSGARITVTLLNAMEQSDTEIGLATMCIGLGQGIATIIERV

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=38.4810126582278, Blast_Score=252, Evalue=4e-67,
Organism=Homo sapiens, GI4501853, Length=396, Percent_Identity=37.6262626262626, Blast_Score=228, Evalue=9e-60,
Organism=Homo sapiens, GI148539872, Length=394, Percent_Identity=35.7868020304569, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI4504327, Length=433, Percent_Identity=33.4872979214781, Blast_Score=188, Evalue=9e-48,
Organism=Homo sapiens, GI4557237, Length=397, Percent_Identity=34.2569269521411, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI194353979, Length=389, Percent_Identity=27.5064267352185, Blast_Score=125, Evalue=9e-29,
Organism=Escherichia coli, GI48994986, Length=386, Percent_Identity=62.1761658031088, Blast_Score=487, Evalue=1e-139,
Organism=Escherichia coli, GI1787663, Length=404, Percent_Identity=42.3267326732673, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1788554, Length=404, Percent_Identity=42.0792079207921, Blast_Score=258, Evalue=7e-70,
Organism=Escherichia coli, GI87082165, Length=396, Percent_Identity=40.1515151515151, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1788683, Length=414, Percent_Identity=30.9178743961353, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI133906874, Length=402, Percent_Identity=36.5671641791045, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17535921, Length=401, Percent_Identity=33.6658354114713, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17551802, Length=428, Percent_Identity=29.2056074766355, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI25147385, Length=400, Percent_Identity=29, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17535917, Length=397, Percent_Identity=26.448362720403, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6322031, Length=399, Percent_Identity=34.8370927318296, Blast_Score=207, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6325229, Length=404, Percent_Identity=33.9108910891089, Blast_Score=199, Evalue=8e-52,
Organism=Drosophila melanogaster, GI24655093, Length=399, Percent_Identity=39.0977443609023, Blast_Score=241, Evalue=7e-64,
Organism=Drosophila melanogaster, GI17648125, Length=400, Percent_Identity=36.25, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI17137578, Length=433, Percent_Identity=31.8706697459584, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24640423, Length=398, Percent_Identity=30.6532663316583, Blast_Score=173, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012805
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 41407; Mature: 41276

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILSPKDVVIVDGVRSAMGKTKNGMFRHVRADSMSAELVRALVERNDFDPRDVEDIIWG
CCCCCCCCEEEECCHHHHHCCCCCCCEEEHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHH
CVNQTLEQGLNIGRNIGLLAGIPKTAGGQTINRLCGSSMQALHTAAAQIMTGQGDVFIIG
HHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHCHHHHHHHHHHHHHEECCCCEEEEE
GVEHMGHVGMMHGVDLNPEASKHYAKASNMMGLTAEMLGRMNNITREEQDAFGLESHRRA
CHHHHCCHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHCCCHHHHHH
WAATTEGRFDNEIIGIEGHDEAGRLQLCTVDEVIRPDATMEQMQKLRPAFDPVGGTVTAA
HEECCCCCCCCEEEEECCCCCCCCEEEEEHHHHHCCCHHHHHHHHHCCCCCCCCCEEEHH
TSSALSDGASAMLIMSAQKAKELGLKPRARIRSMAIAGCDAAIMGYGPVPATQKALKRAG
HHHHHHCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCC
MSIDDMQTIELNEAFAAQGLSVLKALNLTDKQDIVNINGGAIALGHPLGCSGARITVTLL
CCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEECCCCCCCCCEEEEHHH
NAMEQSDTEIGLATMCIGLGQGIATIIERV
HHHHHCCCHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure 
TILSPKDVVIVDGVRSAMGKTKNGMFRHVRADSMSAELVRALVERNDFDPRDVEDIIWG
CCCCCCCEEEECCHHHHHCCCCCCCEEEHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHH
CVNQTLEQGLNIGRNIGLLAGIPKTAGGQTINRLCGSSMQALHTAAAQIMTGQGDVFIIG
HHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHCHHHHHHHHHHHHHEECCCCEEEEE
GVEHMGHVGMMHGVDLNPEASKHYAKASNMMGLTAEMLGRMNNITREEQDAFGLESHRRA
CHHHHCCHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHCCCHHHHHH
WAATTEGRFDNEIIGIEGHDEAGRLQLCTVDEVIRPDATMEQMQKLRPAFDPVGGTVTAA
HEECCCCCCCCEEEEECCCCCCCCEEEEEHHHHHCCCHHHHHHHHHCCCCCCCCCEEEHH
TSSALSDGASAMLIMSAQKAKELGLKPRARIRSMAIAGCDAAIMGYGPVPATQKALKRAG
HHHHHHCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCC
MSIDDMQTIELNEAFAAQGLSVLKALNLTDKQDIVNINGGAIALGHPLGCSGARITVTLL
CCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEECCCCCCCCCEEEEHHH
NAMEQSDTEIGLATMCIGLGQGIATIIERV
HHHHHCCCHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA