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The map label for this gene is pilG [H]

Identifier: 93006925

GI number: 93006925

Start: 2574107

End: 2574484

Strand: Reverse

Name: pilG [H]

Synonym: Pcryo_2101

Alternate gene names: 93006925

Gene position: 2574484-2574107 (Counterclockwise)

Preceding gene: 93006933

Following gene: 93006924

Centisome position: 84.14

GC content: 37.83

Gene sequence:

>378_bases
ATGGAAAATAATTTTGATGGTTTAAAAGTAATGGTTATTGATGACTCAAAAACCATTCGCCGTACCGCAGAAACCTTGCT
GCAAAAAGCGGGCTGTGAAGTTGTCACTGCCATTGATGGATTTGATGCTTTGGCTAAGATTGTCGACCACAATCCAGATA
TTATTTTTGTGGATATCATGATGCCACGTTTGGACGGTTATCAAACTTGTGCCTTGATTAAAAATAACCCTGACTATGCC
AGTAAACCTGTGATTATGTTGTCATCTAAAGATGGTTTATTTGATAAAGCCCGTGGACGTATTGTAGGTTCTGATGAATA
TCTGACCAAACCGTTTAGTAAGGATGAGCTTTTTGAGGCAATTAATCAGTATCGTTAA

Upstream 100 bases:

>100_bases
ACATACTAAGATAATTCATTGTGAGTAAACAGTACTACGCGTGAGTAGTACTGTGAACATATCGCATAATGAATAAACAG
TCGTTAATAGGACAATAAGA

Downstream 100 bases:

>100_bases
TCTGTAATATGATCATAAAAAGTGGTCAAGGCTTTTTCATATAACGATAAAATATTGTTGTCATAATCAGTAATATGCAT
TCAGTTCAGCATGAAATATT

Product: response regulator receiver domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MENNFDGLKVMVIDDSKTIRRTAETLLQKAGCEVVTAIDGFDALAKIVDHNPDIIFVDIMMPRLDGYQTCALIKNNPDYA
SKPVIMLSSKDGLFDKARGRIVGSDEYLTKPFSKDELFEAINQYR

Sequences:

>Translated_125_residues
MENNFDGLKVMVIDDSKTIRRTAETLLQKAGCEVVTAIDGFDALAKIVDHNPDIIFVDIMMPRLDGYQTCALIKNNPDYA
SKPVIMLSSKDGLFDKARGRIVGSDEYLTKPFSKDELFEAINQYR
>Mature_125_residues
MENNFDGLKVMVIDDSKTIRRTAETLLQKAGCEVVTAIDGFDALAKIVDHNPDIIFVDIMMPRLDGYQTCALIKNNPDYA
SKPVIMLSSKDGLFDKARGRIVGSDEYLTKPFSKDELFEAINQYR

Specific function: Is involved in pilus biosynthesis and twitching motility. May act as a single-domain response regulator whose function may be to receive a certain environmental signal(s) and then transduce that signal to the pilus assembly machinery via specific protein-

COG id: COG0784

COG function: function code T; FOG: CheY-like receiver

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=114, Percent_Identity=32.4561403508772, Blast_Score=67, Evalue=4e-13,
Organism=Escherichia coli, GI87082012, Length=114, Percent_Identity=30.7017543859649, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI1787487, Length=111, Percent_Identity=28.8288288288288, Blast_Score=64, Evalue=4e-12,
Organism=Escherichia coli, GI1787375, Length=111, Percent_Identity=30.6306306306306, Blast_Score=62, Evalue=1e-11,
Organism=Escherichia coli, GI1786911, Length=108, Percent_Identity=32.4074074074074, Blast_Score=59, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 14050; Mature: 14050

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENNFDGLKVMVIDDSKTIRRTAETLLQKAGCEVVTAIDGFDALAKIVDHNPDIIFVDIM
CCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCEEEEEEE
MPRLDGYQTCALIKNNPDYASKPVIMLSSKDGLFDKARGRIVGSDEYLTKPFSKDELFEA
ECCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCHHCCCEEECCCCHHCCCCCHHHHHHH
INQYR
HHHCC
>Mature Secondary Structure
MENNFDGLKVMVIDDSKTIRRTAETLLQKAGCEVVTAIDGFDALAKIVDHNPDIIFVDIM
CCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCEEEEEEE
MPRLDGYQTCALIKNNPDYASKPVIMLSSKDGLFDKARGRIVGSDEYLTKPFSKDELFEA
ECCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCHHCCCEEECCCCHHCCCCCHHHHHHH
INQYR
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8104179; 10984043 [H]