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The map label for this gene is ddl [H]

Identifier: 93006854

GI number: 93006854

Start: 2495033

End: 2496172

Strand: Reverse

Name: ddl [H]

Synonym: Pcryo_2030

Alternate gene names: 93006854

Gene position: 2496172-2495033 (Counterclockwise)

Preceding gene: 93006855

Following gene: 93006853

Centisome position: 81.58

GC content: 44.3

Gene sequence:

>1140_bases
ATGAATAATACTAAAGAAAATAATAATATTGATGAAGATGGCGTTAATGTTGAGCAAAACGCTACAGATAACAGTAACAA
CAACATTAAAGGCAATGTTCAAAATAACACTGCCCCTATTATCAATCCAGAAACAGCTTTAGCAGCTGCTGCACAAGCAG
AGCAGGATAAAGTAAATAACAATAGCCAAAGCGCTAATACGTTACCCGCTATTAAAGAAAGTAAAGTAAAAGAGGGGAAT
GAGTTTGGTAGAGTTGCTGTCATTTACGGTGGGAATAGTAATGAGCGTAGCGTGTCTTTAGACAGCGGTGCAGCTGTATT
GCAAGCACTACAAAACCAAGGGGTTGATGCCACGCATTTTGATCCTAAGCATCAAGACATTACTGAACTACGTCAGTATG
ATCGTGTATTTAATGTCTTACATGGTCGGGGTGGCGAAGATGGTCTGCTGCAAGGCGTGCTACAGTGGTTTGATATTCCT
CAAACCGGCTCAGGTATTTTAGCGTCAGCACTTGGTATGGATAAAGTACGTACCAAACAGCTGTGGCAAGGCTGCGGATT
ATCGACTGCACCGTTTTCACTGCTAACCGCTGATACCGACTGGCAGCAGGTGGTTAATATGTTAGGACTACCCCTGATTA
TCAAGCCTGTTCATGAAGGCTCAAGCATTGGTATGACTAAGGTTAATAACCTTGATGAGCTTCCTGCTGCCTATGCAACC
GCCGTACAATGTGGCGATGTAGTGATGGCGGAGCGCTGGATAACTGGTCGCGAGTTTACTATCGTTATCATTGATGATGA
AGCTTATCCTGTCATTCGCCTAGAGCCTGCGGATATCACCAATTTTTATGATTTCGAAGCCAAGTATAATCGTAACGATA
CCAGCTACTATATCCCTTGTGGTTTAAGTGCTGCGGATGAAAAGCATTTGCAAGATCTAAGTTTATCGGCGTTTCGTGCC
GTTGATGCAAAAGGCTGGGGACGTATCGATGCGATGCAAGACGAAGCGGGCAACTTTTGGCTACTTGAGATTAATACCGT
ACCGGGCATGACCAGTCATAGTTTGGTACCCATGGCAGCCAAAGCTCGTGGCATGGATTTCGAGAGCTTGTGCTGGCATA
TTTTGGCGCAAACGGTGTAA

Upstream 100 bases:

>100_bases
ATGTCGGGCAAATAGCTATCGATCTCGCCGCCAATAATCTTTATATCAAATAAGCCTTAGAAGCATTGCTTTAAAAATAT
TAGGAGCACTAACAATGACC

Downstream 100 bases:

>100_bases
CACCTTATTAAATAAGTCACTGCCTATATACAACAAGCTGTTCTTAGTAATTATACAAGAGTGATTATTTCAATAAATGA
GTTATTTAAACTATTTATCA

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MNNTKENNNIDEDGVNVEQNATDNSNNNIKGNVQNNTAPIINPETALAAAAQAEQDKVNNNSQSANTLPAIKESKVKEGN
EFGRVAVIYGGNSNERSVSLDSGAAVLQALQNQGVDATHFDPKHQDITELRQYDRVFNVLHGRGGEDGLLQGVLQWFDIP
QTGSGILASALGMDKVRTKQLWQGCGLSTAPFSLLTADTDWQQVVNMLGLPLIIKPVHEGSSIGMTKVNNLDELPAAYAT
AVQCGDVVMAERWITGREFTIVIIDDEAYPVIRLEPADITNFYDFEAKYNRNDTSYYIPCGLSAADEKHLQDLSLSAFRA
VDAKGWGRIDAMQDEAGNFWLLEINTVPGMTSHSLVPMAAKARGMDFESLCWHILAQTV

Sequences:

>Translated_379_residues
MNNTKENNNIDEDGVNVEQNATDNSNNNIKGNVQNNTAPIINPETALAAAAQAEQDKVNNNSQSANTLPAIKESKVKEGN
EFGRVAVIYGGNSNERSVSLDSGAAVLQALQNQGVDATHFDPKHQDITELRQYDRVFNVLHGRGGEDGLLQGVLQWFDIP
QTGSGILASALGMDKVRTKQLWQGCGLSTAPFSLLTADTDWQQVVNMLGLPLIIKPVHEGSSIGMTKVNNLDELPAAYAT
AVQCGDVVMAERWITGREFTIVIIDDEAYPVIRLEPADITNFYDFEAKYNRNDTSYYIPCGLSAADEKHLQDLSLSAFRA
VDAKGWGRIDAMQDEAGNFWLLEINTVPGMTSHSLVPMAAKARGMDFESLCWHILAQTV
>Mature_379_residues
MNNTKENNNIDEDGVNVEQNATDNSNNNIKGNVQNNTAPIINPETALAAAAQAEQDKVNNNSQSANTLPAIKESKVKEGN
EFGRVAVIYGGNSNERSVSLDSGAAVLQALQNQGVDATHFDPKHQDITELRQYDRVFNVLHGRGGEDGLLQGVLQWFDIP
QTGSGILASALGMDKVRTKQLWQGCGLSTAPFSLLTADTDWQQVVNMLGLPLIIKPVHEGSSIGMTKVNNLDELPAAYAT
AVQCGDVVMAERWITGREFTIVIIDDEAYPVIRLEPADITNFYDFEAKYNRNDTSYYIPCGLSAADEKHLQDLSLSAFRA
VDAKGWGRIDAMQDEAGNFWLLEINTVPGMTSHSLVPMAAKARGMDFESLCWHILAQTV

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=301, Percent_Identity=50.8305647840532, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1786579, Length=343, Percent_Identity=28.8629737609329, Blast_Score=138, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 41384; Mature: 41384

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNTKENNNIDEDGVNVEQNATDNSNNNIKGNVQNNTAPIINPETALAAAAQAEQDKVNN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEECCHHHHHHHHHHHHHHCCC
NSQSANTLPAIKESKVKEGNEFGRVAVIYGGNSNERSVSLDSGAAVLQALQNQGVDATHF
CCCCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCCCEEECCCHHHHHHHHHHCCCCCCCC
DPKHQDITELRQYDRVFNVLHGRGGEDGLLQGVLQWFDIPQTGSGILASALGMDKVRTKQ
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCHHHHHHHH
LWQGCGLSTAPFSLLTADTDWQQVVNMLGLPLIIKPVHEGSSIGMTKVNNLDELPAAYAT
HHHCCCCCCCCCEEEECCCCHHHHHHHCCCCEEEEECCCCCCCCCEECCCCHHHHHHHHH
AVQCGDVVMAERWITGREFTIVIIDDEAYPVIRLEPADITNFYDFEAKYNRNDTSYYIPC
HHHHCCCHHHHHCCCCCEEEEEEECCCCCCEEEECCCCCCCEEECCEEECCCCCCEEEEC
GLSAADEKHLQDLSLSAFRAVDAKGWGRIDAMQDEAGNFWLLEINTVPGMTSHSLVPMAA
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHH
KARGMDFESLCWHILAQTV
HHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNNTKENNNIDEDGVNVEQNATDNSNNNIKGNVQNNTAPIINPETALAAAAQAEQDKVNN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEECCHHHHHHHHHHHHHHCCC
NSQSANTLPAIKESKVKEGNEFGRVAVIYGGNSNERSVSLDSGAAVLQALQNQGVDATHF
CCCCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCCCEEECCCHHHHHHHHHHCCCCCCCC
DPKHQDITELRQYDRVFNVLHGRGGEDGLLQGVLQWFDIPQTGSGILASALGMDKVRTKQ
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCHHHHHHHH
LWQGCGLSTAPFSLLTADTDWQQVVNMLGLPLIIKPVHEGSSIGMTKVNNLDELPAAYAT
HHHCCCCCCCCCEEEECCCCHHHHHHHCCCCEEEEECCCCCCCCCEECCCCHHHHHHHHH
AVQCGDVVMAERWITGREFTIVIIDDEAYPVIRLEPADITNFYDFEAKYNRNDTSYYIPC
HHHHCCCHHHHHCCCCCEEEEEEECCCCCCEEEECCCCCCCEEECCEEECCCCCCEEEEC
GLSAADEKHLQDLSLSAFRAVDAKGWGRIDAMQDEAGNFWLLEINTVPGMTSHSLVPMAA
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHH
KARGMDFESLCWHILAQTV
HHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA