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The map label for this gene is purU [H]

Identifier: 93006811

GI number: 93006811

Start: 2447115

End: 2448038

Strand: Reverse

Name: purU [H]

Synonym: Pcryo_1987

Alternate gene names: 93006811

Gene position: 2448038-2447115 (Counterclockwise)

Preceding gene: 93006812

Following gene: 93006809

Centisome position: 80.0

GC content: 44.26

Gene sequence:

>924_bases
ATGTCAGACACCGCAACGATTGCTTCACCAAAAACGTCACTTCAAATGACACCTAAAGAGATTCCTACTATTACTGCTAC
AGCGACCCTATTGATTAAATGCCGTGACCAAGCCGGTATCGTACAAGCCGTATCTGAGTTTATTCATCGCTATGGCGGCA
ATATCATTACGTTAGATCAGTACTCAACGGCCCATGAAGGCGGTCAATACTTTATGCGTTTAGAGTTCGCTTTAGCAGGA
TTGAGTGAGATTATCGATAACTTTGAAGCCAGCTTTGCGCATACCGTTGCCAAACGCCACAGTATGGAATGGCGCTTACA
TGACAATGCGATTAAGACCAAAGTTGGTATCTTGGTATCTAAATTTGATCATGCCTTATTAGACTTACTATGGCGTCATC
AGCGCGGCTTGCTCGATTGCGATATTACCTGCGTGGTAAGTAATCATATTGTTTTACGCCAATCCGTAGAAAACTTTGGT
ATTGCTTTTCATCACGTTCCAGTGACCAAAGATAATAAAGTAGACGCAGAAGAGAAGATCCATACATTGATGGCGGGTAA
TGATTTATTGGTGCTAGCGCGCTATATGCAGATATTGTCGTCAGACTTTGTTAAGCGCTGGCCGATGCAGATTATCAATA
TTCATCATTCGTTCTTGCCTGCGTTCGTCGGTGCTGACCCTTATCGTCAAGCTTATGATAAAGGGGTGAAATTGATTGGC
GCAACTGCCCATTATGTGACTGCTGAGCTCGATCAAGGACCTATCATTGAGCAAGATGTGCACCGCGTCACCCATCGTCA
AGGTGTCACTGAACTGCGCGCTATTGGTCGTGATATCGAGCGTAATGTATTGGCACGCGCAGTCAATTGGCATGTACAAA
ATCGCGTGATTGTCGCAGGGAATAAGACGGTAGTATTTAATTAG

Upstream 100 bases:

>100_bases
AATCAGCAAATTCTTGCAAAAACGATTAGAATAGTCGGTTTCCTATGCTGTTAGCTATATTCAAAGTAAGCAGCAAGTTT
TATTTTTAATTGAGCGTACT

Downstream 100 bases:

>100_bases
GTTTTCATTAGAGTGGTTTGAAATTTAGAACAGAGTATTAAAAGATATTTAATAAAAAAGCTGCGAGTATTATTATTTAA
TACTCGCAGCTTTTTACTAT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSDTATIASPKTSLQMTPKEIPTITATATLLIKCRDQAGIVQAVSEFIHRYGGNIITLDQYSTAHEGGQYFMRLEFALAG
LSEIIDNFEASFAHTVAKRHSMEWRLHDNAIKTKVGILVSKFDHALLDLLWRHQRGLLDCDITCVVSNHIVLRQSVENFG
IAFHHVPVTKDNKVDAEEKIHTLMAGNDLLVLARYMQILSSDFVKRWPMQIINIHHSFLPAFVGADPYRQAYDKGVKLIG
ATAHYVTAELDQGPIIEQDVHRVTHRQGVTELRAIGRDIERNVLARAVNWHVQNRVIVAGNKTVVFN

Sequences:

>Translated_307_residues
MSDTATIASPKTSLQMTPKEIPTITATATLLIKCRDQAGIVQAVSEFIHRYGGNIITLDQYSTAHEGGQYFMRLEFALAG
LSEIIDNFEASFAHTVAKRHSMEWRLHDNAIKTKVGILVSKFDHALLDLLWRHQRGLLDCDITCVVSNHIVLRQSVENFG
IAFHHVPVTKDNKVDAEEKIHTLMAGNDLLVLARYMQILSSDFVKRWPMQIINIHHSFLPAFVGADPYRQAYDKGVKLIG
ATAHYVTAELDQGPIIEQDVHRVTHRQGVTELRAIGRDIERNVLARAVNWHVQNRVIVAGNKTVVFN
>Mature_306_residues
SDTATIASPKTSLQMTPKEIPTITATATLLIKCRDQAGIVQAVSEFIHRYGGNIITLDQYSTAHEGGQYFMRLEFALAGL
SEIIDNFEASFAHTVAKRHSMEWRLHDNAIKTKVGILVSKFDHALLDLLWRHQRGLLDCDITCVVSNHIVLRQSVENFGI
AFHHVPVTKDNKVDAEEKIHTLMAGNDLLVLARYMQILSSDFVKRWPMQIINIHHSFLPAFVGADPYRQAYDKGVKLIGA
TAHYVTAELDQGPIIEQDVHRVTHRQGVTELRAIGRDIERNVLARAVNWHVQNRVIVAGNKTVVFN

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=119, Percent_Identity=31.9327731092437, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI209869995, Length=119, Percent_Identity=31.9327731092437, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI209869993, Length=119, Percent_Identity=31.9327731092437, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1787483, Length=275, Percent_Identity=40, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1788846, Length=140, Percent_Identity=32.8571428571429, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17567511, Length=154, Percent_Identity=35.7142857142857, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24582400, Length=183, Percent_Identity=31.1475409836066, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 34532; Mature: 34401

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTATIASPKTSLQMTPKEIPTITATATLLIKCRDQAGIVQAVSEFIHRYGGNIITLDQ
CCCCCCCCCCCCCEEECHHHCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCEEEECC
YSTAHEGGQYFMRLEFALAGLSEIIDNFEASFAHTVAKRHSMEWRLHDNAIKTKVGILVS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHH
KFDHALLDLLWRHQRGLLDCDITCVVSNHIVLRQSVENFGIAFHHVPVTKDNKVDAEEKI
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEHHHHHHCEEEEEECCCCCCCCCHHHHH
HTLMAGNDLLVLARYMQILSSDFVKRWPMQIINIHHSFLPAFVGADPYRQAYDKGVKLIG
HHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEE
ATAHYVTAELDQGPIIEQDVHRVTHRQGVTELRAIGRDIERNVLARAVNWHVQNRVIVAG
CCHHEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEECCEEEEEC
NKTVVFN
CEEEEEC
>Mature Secondary Structure 
SDTATIASPKTSLQMTPKEIPTITATATLLIKCRDQAGIVQAVSEFIHRYGGNIITLDQ
CCCCCCCCCCCCEEECHHHCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHCCCEEEECC
YSTAHEGGQYFMRLEFALAGLSEIIDNFEASFAHTVAKRHSMEWRLHDNAIKTKVGILVS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHH
KFDHALLDLLWRHQRGLLDCDITCVVSNHIVLRQSVENFGIAFHHVPVTKDNKVDAEEKI
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEHHHHHHCEEEEEECCCCCCCCCHHHHH
HTLMAGNDLLVLARYMQILSSDFVKRWPMQIINIHHSFLPAFVGADPYRQAYDKGVKLIG
HHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEE
ATAHYVTAELDQGPIIEQDVHRVTHRQGVTELRAIGRDIERNVLARAVNWHVQNRVIVAG
CCHHEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEECCEEEEEC
NKTVVFN
CEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]