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The map label for this gene is yfcB [C]

Identifier: 93006460

GI number: 93006460

Start: 2012145

End: 2013320

Strand: Reverse

Name: yfcB [C]

Synonym: Pcryo_1636

Alternate gene names: 93006460

Gene position: 2013320-2012145 (Counterclockwise)

Preceding gene: 93006466

Following gene: 93006459

Centisome position: 65.8

GC content: 43.11

Gene sequence:

>1176_bases
ATGTCAGAAGACCAAGTAATGAGCGCAGAAGAATTTCAAAATCAATACTTTAATGATGATGAGTCACAAGGTATCAATGA
TGATATGGAATACGATTTAGAGACAGGGCTGGTCGGTGAGCACGATGAATTTGCGACTTTGCGTGCGGAGCTTGAAGAGG
CACGTGAGCAACTGGTCACTATTCGTGACTTTATTCGTTTTTCAGTGACGCAATTACGTAACTATGATGTGGTTGTCGCC
CAAGGCACCACCGATGAGTTTGCCGAAGCATCTGCGATTGTATTGCATTCTTTATCTTTGGATTGGTCCGCCAATGATCA
GATTCTGGACTGTCGTTTGACCACCTCAGAGAAGCAATTGGTTCTAAGCTTGTTAGAAGAGCGTATCGCTGAGCGTAAGC
CATTAAGCTATTTGATTAATCTGTCTTATTTCTGTGATTTGCCGTTCTATGTCGATGAGCGCGTATTGATTCCTCGTTCA
CCTATTGCTGAATTGATTCGTCAGCAGTTCCACCCATATTTTGAAGTGAGCGAGCTGGCAAAACCAATAGGCGTAAGTGT
CAATAATCAAGCGCCGGCTTTTTATGATCATGGGTTAGAAATTAAACAGCTATCACAGCCTGAGCGTATTTTAGATTTAT
GTACCGGTTCTGGCTGTATTGCTATCGCACTGGCGACTCGCTTTGTCGATGCATTGGTTGATGCTGTAGATATTGATAAA
GGCGCGCTAGAAGTCGCGATGGTTAATGTCGATCATCATGACCTTGGTCATCAAGTCAATGTAATTGAGTCTGACTTATT
TGCCAAAATCCCTGCCGAAAATCAATATGAGCTGATTGTAACCAATCCTCCTTATGTTGATGCCGCTATCATGGCAGATT
TGCCACCAGAGTTTTTATATGAGCCTGAGCATGCACTCGCTGCTGGTCAAGATGGTTTGGACTTGGTACATCGTATCTTG
TTTGAAGCGCCAGATTATCTTAGCCCAGAAGGTTTACTAGTCTGTGAAGTGGGCGATAGCGAGTGGGCGTTAAAACAAGC
TTATCCTGAGATTCAGTTTGATTGGCTAAGGTTTGCTCATGGCGGTCATGGCATCTTTGCGATTACCTATGATGAGCTTA
TGAGCTATCGCTCATTATTCGCCAATTATGTACAGTTACTAGATAGCAATCAATAA

Upstream 100 bases:

>100_bases
CGAGATTTTTATTTAATAAATTGGCTGTTCAGTGAAATCATTGAAAGCGATTTAACCCATCCGCATGTAGTCAGTGAGCA
TGTAATCAGCAAGGTGCGAT

Downstream 100 bases:

>100_bases
ATCACGGTTGATACTATAATTCAAACAGTCAAAAATCGACTTCATTTGTTTAAGGATAAATATGGCAGGCAACAGTATTG
GGCAGGTCTTTACCGTCACC

Product: N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MSEDQVMSAEEFQNQYFNDDESQGINDDMEYDLETGLVGEHDEFATLRAELEEAREQLVTIRDFIRFSVTQLRNYDVVVA
QGTTDEFAEASAIVLHSLSLDWSANDQILDCRLTTSEKQLVLSLLEERIAERKPLSYLINLSYFCDLPFYVDERVLIPRS
PIAELIRQQFHPYFEVSELAKPIGVSVNNQAPAFYDHGLEIKQLSQPERILDLCTGSGCIAIALATRFVDALVDAVDIDK
GALEVAMVNVDHHDLGHQVNVIESDLFAKIPAENQYELIVTNPPYVDAAIMADLPPEFLYEPEHALAAGQDGLDLVHRIL
FEAPDYLSPEGLLVCEVGDSEWALKQAYPEIQFDWLRFAHGGHGIFAITYDELMSYRSLFANYVQLLDSNQ

Sequences:

>Translated_391_residues
MSEDQVMSAEEFQNQYFNDDESQGINDDMEYDLETGLVGEHDEFATLRAELEEAREQLVTIRDFIRFSVTQLRNYDVVVA
QGTTDEFAEASAIVLHSLSLDWSANDQILDCRLTTSEKQLVLSLLEERIAERKPLSYLINLSYFCDLPFYVDERVLIPRS
PIAELIRQQFHPYFEVSELAKPIGVSVNNQAPAFYDHGLEIKQLSQPERILDLCTGSGCIAIALATRFVDALVDAVDIDK
GALEVAMVNVDHHDLGHQVNVIESDLFAKIPAENQYELIVTNPPYVDAAIMADLPPEFLYEPEHALAAGQDGLDLVHRIL
FEAPDYLSPEGLLVCEVGDSEWALKQAYPEIQFDWLRFAHGGHGIFAITYDELMSYRSLFANYVQLLDSNQ
>Mature_390_residues
SEDQVMSAEEFQNQYFNDDESQGINDDMEYDLETGLVGEHDEFATLRAELEEAREQLVTIRDFIRFSVTQLRNYDVVVAQ
GTTDEFAEASAIVLHSLSLDWSANDQILDCRLTTSEKQLVLSLLEERIAERKPLSYLINLSYFCDLPFYVDERVLIPRSP
IAELIRQQFHPYFEVSELAKPIGVSVNNQAPAFYDHGLEIKQLSQPERILDLCTGSGCIAIALATRFVDALVDAVDIDKG
ALEVAMVNVDHHDLGHQVNVIESDLFAKIPAENQYELIVTNPPYVDAAIMADLPPEFLYEPEHALAAGQDGLDLVHRILF
EAPDYLSPEGLLVCEVGDSEWALKQAYPEIQFDWLRFAHGGHGIFAITYDELMSYRSLFANYVQLLDSNQ

Specific function: Unknown

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082085, Length=333, Percent_Identity=45.6456456456456, Blast_Score=271, Evalue=5e-74,
Organism=Escherichia coli, GI1787463, Length=241, Percent_Identity=29.8755186721992, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR017127
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 44190; Mature: 44059

Theoretical pI: Translated: 3.99; Mature: 3.99

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDQVMSAEEFQNQYFNDDESQGINDDMEYDLETGLVGEHDEFATLRAELEEAREQLVT
CCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
IRDFIRFSVTQLRNYDVVVAQGTTDEFAEASAIVLHSLSLDWSANDQILDCRLTTSEKQL
HHHHHHHHHHHHCCCEEEEECCCCHHHHHHHEEEEEEEECCCCCCCCEEEEEECCCHHHH
VLSLLEERIAERKPLSYLINLSYFCDLPFYVDERVLIPRSPIAELIRQQFHPYFEVSELA
HHHHHHHHHHHCCCHHHEEEEHHHCCCCCEECCEEECCCCHHHHHHHHHCCCCEEHHHHH
KPIGVSVNNQAPAFYDHGLEIKQLSQPERILDLCTGSGCIAIALATRFVDALVDAVDIDK
HHCCCCCCCCCCCHHHCCCCHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
GALEVAMVNVDHHDLGHQVNVIESDLFAKIPAENQYELIVTNPPYVDAAIMADLPPEFLY
CCEEEEEEECCCCCCCCEEEHHHHHHHEECCCCCCEEEEECCCCCCCHHHHHCCCHHHHC
EPEHALAAGQDGLDLVHRILFEAPDYLSPEGLLVCEVGDSEWALKQAYPEIQFDWLRFAH
CCCHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCEEHHHHHCC
GGHGIFAITYDELMSYRSLFANYVQLLDSNQ
CCCEEEEEEHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SEDQVMSAEEFQNQYFNDDESQGINDDMEYDLETGLVGEHDEFATLRAELEEAREQLVT
CCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
IRDFIRFSVTQLRNYDVVVAQGTTDEFAEASAIVLHSLSLDWSANDQILDCRLTTSEKQL
HHHHHHHHHHHHCCCEEEEECCCCHHHHHHHEEEEEEEECCCCCCCCEEEEEECCCHHHH
VLSLLEERIAERKPLSYLINLSYFCDLPFYVDERVLIPRSPIAELIRQQFHPYFEVSELA
HHHHHHHHHHHCCCHHHEEEEHHHCCCCCEECCEEECCCCHHHHHHHHHCCCCEEHHHHH
KPIGVSVNNQAPAFYDHGLEIKQLSQPERILDLCTGSGCIAIALATRFVDALVDAVDIDK
HHCCCCCCCCCCCHHHCCCCHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
GALEVAMVNVDHHDLGHQVNVIESDLFAKIPAENQYELIVTNPPYVDAAIMADLPPEFLY
CCEEEEEEECCCCCCCCEEEHHHHHHHEECCCCCCEEEEECCCCCCCHHHHHCCCHHHHC
EPEHALAAGQDGLDLVHRILFEAPDYLSPEGLLVCEVGDSEWALKQAYPEIQFDWLRFAH
CCCHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCEEHHHHHCC
GGHGIFAITYDELMSYRSLFANYVQLLDSNQ
CCCEEEEEEHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]