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The map label for this gene is fadA [H]

Identifier: 93006364

GI number: 93006364

Start: 1890061

End: 1891269

Strand: Direct

Name: fadA [H]

Synonym: Pcryo_1538

Alternate gene names: 93006364

Gene position: 1890061-1891269 (Clockwise)

Preceding gene: 93006363

Following gene: 93006365

Centisome position: 61.77

GC content: 48.8

Gene sequence:

>1209_bases
ATGACCCAGACCGCTTATATCTATGATGCCATTCGCACCCCACGTGGCAAAGGCAAAAAAGATGGCTCCCTCCATCAGGC
GTCACCTATCTGGCTGATTCGCACGCTACTAAAAGAGATGCAACAACGCCATAATTTAGATACCAGTTTGGTAGACGACG
TCATTTTAGGCTGTGTGACACCAGTCGGTGAGCAGGGTTCTGATATCGCACGTATCGCCGTTATCGACGCTGATTGGCAT
GAGAGCGTAGCAGGGTTAACGCTGTCACGCTTTTGTGCGTCTGGTCTCGAGTCAATTAACCTTGCGGCAACCAAAGTCAT
GTCTGGTATGGAAGACTTAGTCGTCGCCGGTGGTGTTGAGTCAATGAGCCGTGTACCAATGGGTATGGACGGCGGCGCTT
GGTATATGGATCCACGAGTAAACGATGCCGTTCAGTTCGTACCTCAAGGGGTTGGCGCTGATACCATCGCAACGCTAAAA
GGCTTTAGTCGTAAAGACGTTGACGAGTTTGCATCAGAATCACACAGAAGAGCAGCGGCTGCATGGGGGAAAGGCTACTA
CGATAAGTCAGTCGTTCCCGTAAAAGATCTAAACGGTTTATTGCTATTAGACACAGACGAAACCATTCGTCCAAACACTT
CAGTGGAAACGCTAGCAGGCCTAAACCCATCATTTATAATGTCAGGCAAAATGGGCTTCGATGGCGTTATTCTTGATAAA
TATACGACGATTGAAAACGTCAATCATGTGCACCACGCTGGTAACTCATCGGGTATCGTTGATGGTTCAGCATTGTGTCT
TGTGGGTAGTAAAGCTGCTGGCAAAAAAGCAGGTCTAAAACCACGCGCTAAAATAACGATGGCATCTGTGATTGGCTCAG
AGCCAACCATCATGTTAACTGGCCCTACCCCTGCCTGCAAAAAAGCACTGGCTAAAGCCGGTATGACTGCTGCTGATATC
GACCTTTGGGAAATCAACGAAGCATTTGCAGCTGTGCCGCTTAATACCGCTGATGACTTTGGTCTCTCGCTTGATAAAGT
GAACGTCAATGGTGGCGCTATCGCTATGGGTCACCCACTGGGTGCGACAGGCGCGATGCTGCTGACCACGGTACTTGATG
AGCTTGAGCGCCGCGATCTAAAAACAGCAATGATTACCCTATGTGTCGGTGGTGGTATGGGTATTGCTACCATTATTGAG
CGTGTTTAA

Upstream 100 bases:

>100_bases
ATTAAGCCAAATGTTCTTAAGTATTTAATTGAGGAGAGTACGTTCTCCTCAAAACTTCATTATCTAACAAATATCCAATG
AAATATTAAAGGGACTTATT

Downstream 100 bases:

>100_bases
GCCTCAAATATTACCTATATATGGACAAACCTACGCATAGAAAATAAGGATATCGAATGAACGCAAACAGTGTAAATGCC
ATTAATGAATTAAACAGTTT

Product: acetyl-CoA acetyltransferase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTQTAYIYDAIRTPRGKGKKDGSLHQASPIWLIRTLLKEMQQRHNLDTSLVDDVILGCVTPVGEQGSDIARIAVIDADWH
ESVAGLTLSRFCASGLESINLAATKVMSGMEDLVVAGGVESMSRVPMGMDGGAWYMDPRVNDAVQFVPQGVGADTIATLK
GFSRKDVDEFASESHRRAAAAWGKGYYDKSVVPVKDLNGLLLLDTDETIRPNTSVETLAGLNPSFIMSGKMGFDGVILDK
YTTIENVNHVHHAGNSSGIVDGSALCLVGSKAAGKKAGLKPRAKITMASVIGSEPTIMLTGPTPACKKALAKAGMTAADI
DLWEINEAFAAVPLNTADDFGLSLDKVNVNGGAIAMGHPLGATGAMLLTTVLDELERRDLKTAMITLCVGGGMGIATIIE
RV

Sequences:

>Translated_402_residues
MTQTAYIYDAIRTPRGKGKKDGSLHQASPIWLIRTLLKEMQQRHNLDTSLVDDVILGCVTPVGEQGSDIARIAVIDADWH
ESVAGLTLSRFCASGLESINLAATKVMSGMEDLVVAGGVESMSRVPMGMDGGAWYMDPRVNDAVQFVPQGVGADTIATLK
GFSRKDVDEFASESHRRAAAAWGKGYYDKSVVPVKDLNGLLLLDTDETIRPNTSVETLAGLNPSFIMSGKMGFDGVILDK
YTTIENVNHVHHAGNSSGIVDGSALCLVGSKAAGKKAGLKPRAKITMASVIGSEPTIMLTGPTPACKKALAKAGMTAADI
DLWEINEAFAAVPLNTADDFGLSLDKVNVNGGAIAMGHPLGATGAMLLTTVLDELERRDLKTAMITLCVGGGMGIATIIE
RV
>Mature_401_residues
TQTAYIYDAIRTPRGKGKKDGSLHQASPIWLIRTLLKEMQQRHNLDTSLVDDVILGCVTPVGEQGSDIARIAVIDADWHE
SVAGLTLSRFCASGLESINLAATKVMSGMEDLVVAGGVESMSRVPMGMDGGAWYMDPRVNDAVQFVPQGVGADTIATLKG
FSRKDVDEFASESHRRAAAAWGKGYYDKSVVPVKDLNGLLLLDTDETIRPNTSVETLAGLNPSFIMSGKMGFDGVILDKY
TTIENVNHVHHAGNSSGIVDGSALCLVGSKAAGKKAGLKPRAKITMASVIGSEPTIMLTGPTPACKKALAKAGMTAADID
LWEINEAFAAVPLNTADDFGLSLDKVNVNGGAIAMGHPLGATGAMLLTTVLDELERRDLKTAMITLCVGGGMGIATIIER
V

Specific function: Subunit Of The Multifunctional Enzyme Complex Of The Fatty Acid Degradation Cycle. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=380, Percent_Identity=36.3157894736842, Blast_Score=211, Evalue=8e-55,
Organism=Homo sapiens, GI167614485, Length=364, Percent_Identity=34.6153846153846, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI4501853, Length=390, Percent_Identity=34.3589743589744, Blast_Score=192, Evalue=6e-49,
Organism=Homo sapiens, GI4557237, Length=410, Percent_Identity=28.5365853658537, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI4504327, Length=444, Percent_Identity=29.5045045045045, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI194353979, Length=124, Percent_Identity=40.3225806451613, Blast_Score=89, Evalue=8e-18,
Organism=Escherichia coli, GI48994986, Length=407, Percent_Identity=39.3120393120393, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1787663, Length=422, Percent_Identity=38.6255924170616, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1788554, Length=415, Percent_Identity=38.0722891566265, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI87082165, Length=409, Percent_Identity=35.2078239608802, Blast_Score=207, Evalue=9e-55,
Organism=Escherichia coli, GI1788683, Length=442, Percent_Identity=26.4705882352941, Blast_Score=118, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI133906874, Length=372, Percent_Identity=33.8709677419355, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI25147385, Length=413, Percent_Identity=31.9612590799031, Blast_Score=177, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17535921, Length=413, Percent_Identity=27.6029055690073, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17551802, Length=445, Percent_Identity=27.8651685393258, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17535917, Length=146, Percent_Identity=32.1917808219178, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322031, Length=402, Percent_Identity=33.5820895522388, Blast_Score=193, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6325229, Length=422, Percent_Identity=31.042654028436, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24655093, Length=410, Percent_Identity=35.8536585365854, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI17648125, Length=416, Percent_Identity=33.6538461538462, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24640423, Length=366, Percent_Identity=31.9672131147541, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI17137578, Length=447, Percent_Identity=30.2013422818792, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 42414; Mature: 42283

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTAYIYDAIRTPRGKGKKDGSLHQASPIWLIRTLLKEMQQRHNLDTSLVDDVILGCVT
CCCCEEEHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
PVGEQGSDIARIAVIDADWHESVAGLTLSRFCASGLESINLAATKVMSGMEDLVVAGGVE
CCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCHH
SMSRVPMGMDGGAWYMDPRVNDAVQFVPQGVGADTIATLKGFSRKDVDEFASESHRRAAA
HHHHCCCCCCCCEEEECCCCCHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHH
AWGKGYYDKSVVPVKDLNGLLLLDTDETIRPNTSVETLAGLNPSFIMSGKMGFDGVILDK
HHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHCCCCCEEEECCCCCCEEEEEC
YTTIENVNHVHHAGNSSGIVDGSALCLVGSKAAGKKAGLKPRAKITMASVIGSEPTIMLT
HHHHHCCHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHEEEHHHHCCCCEEEEE
GPTPACKKALAKAGMTAADIDLWEINEAFAAVPLNTADDFGLSLDKVNVNGGAIAMGHPL
CCCHHHHHHHHHCCCCCCCCCCEECCCHHEEECCCCCCCCCCEEEEEECCCCEEEECCCC
GATGAMLLTTVLDELERRDLKTAMITLCVGGGMGIATIIERV
CCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHCC
>Mature Secondary Structure 
TQTAYIYDAIRTPRGKGKKDGSLHQASPIWLIRTLLKEMQQRHNLDTSLVDDVILGCVT
CCCEEEHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
PVGEQGSDIARIAVIDADWHESVAGLTLSRFCASGLESINLAATKVMSGMEDLVVAGGVE
CCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCHH
SMSRVPMGMDGGAWYMDPRVNDAVQFVPQGVGADTIATLKGFSRKDVDEFASESHRRAAA
HHHHCCCCCCCCEEEECCCCCHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHH
AWGKGYYDKSVVPVKDLNGLLLLDTDETIRPNTSVETLAGLNPSFIMSGKMGFDGVILDK
HHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHCCCCCEEEECCCCCCEEEEEC
YTTIENVNHVHHAGNSSGIVDGSALCLVGSKAAGKKAGLKPRAKITMASVIGSEPTIMLT
HHHHHCCHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHEEEHHHHCCCCEEEEE
GPTPACKKALAKAGMTAADIDLWEINEAFAAVPLNTADDFGLSLDKVNVNGGAIAMGHPL
CCCHHHHHHHHHCCCCCCCCCCEECCCHHEEECCCCCCCCCCEEEEEECCCCEEEECCCC
GATGAMLLTTVLDELERRDLKTAMITLCVGGGMGIATIIERV
CCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: Acyl group transfer; Claisen condensation [C]

Inhibitor: Tris (hydroxy methyl) aminomethane [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]