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The map label for this gene is rnhB

Identifier: 93006331

GI number: 93006331

Start: 1849337

End: 1850197

Strand: Reverse

Name: rnhB

Synonym: Pcryo_1505

Alternate gene names: 93006331

Gene position: 1850197-1849337 (Counterclockwise)

Preceding gene: 93006332

Following gene: 93006330

Centisome position: 60.47

GC content: 43.9

Gene sequence:

>861_bases
ATGAATAGCATGCTACATATAGAGAGTAATCTTTTACAGATTGAACGCAGTATCGAACAGATTGATATAAAAGGACAATA
TATTAATTTGGCTTCTCCCATTTATTTATCTGGTCAACTTAATATCAACGAATTACTTGCGCAATATTCGAATAATATTG
CACAGAGTGATACGAGTTCAGTGCTTCAGATTGGTGTCGATGAAGCAGGGCGTGGACCATTGTTGGGCAGTGTGAATGTT
GCGTCGGCAATATTACCACGTGCTTGGTCAGGACTGATTGAAGGGCAGCCTCTCAAAGATACGCCCTTATCGATACTGAC
GGACTCCAAGCAGTTGAGCGAAAAAAAGCGCGATATGCTGTATCCCTTGGTTCAGCAACATGCCATTGGTTATGTCATTG
CTGATATTCCAGCGGCAGTGATTGACCAAGTGAATATCTTACAAGCCACCATGCTTGGGATGCGTTTATGTACTGAGGTA
ATGTTAGAGGTCATTGCTTATCATTTGAATGACATGGCACAAGACGTACACCAGCTGCATGCTGAAGTTTTATTCGATGG
GAATCGCTGTCCTGAATTGAATTATGCAAGGCTCGTAGAACTTGGTGTCGAAGAAAAAGTTGACTGTCAAGCATGGATCA
AGGGTGATGCTCGTCATACCAGTATTGCCGCCGCCAGTATTTTGGCAAAAGTTAGCCGTGACAAAACCATGTACGCGCTT
GACGCTCAGCATCCTGAATATGGCATTGCCAAGCATAAAGGCTATCCAACACGCGCACATATGGAGGCAATCGAAACTTA
TGGCGTATTAGCCGCGCATAGACGCAGCTTTGCACCCGTTAGAAAGGCGTTAAAGCGTTAA

Upstream 100 bases:

>100_bases
AACAATGGTATCTGCAAAATTATCCGTCGAAAACGCCGCAGATCTAGTAAGAAAATTCATTACATACCTTTTCATTTCAT
AAAATAAGACAATAAAATTA

Downstream 100 bases:

>100_bases
GAGTGGGTAGAGATTGGCTGATTTATTTAAATAAATAGCCTAAGCGAAATACTTGTTAGTTTTTTAGTAATCATGATTTA
AAGTTTTATACTTTTTATTA

Product: ribonuclease H

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MNSMLHIESNLLQIERSIEQIDIKGQYINLASPIYLSGQLNINELLAQYSNNIAQSDTSSVLQIGVDEAGRGPLLGSVNV
ASAILPRAWSGLIEGQPLKDTPLSILTDSKQLSEKKRDMLYPLVQQHAIGYVIADIPAAVIDQVNILQATMLGMRLCTEV
MLEVIAYHLNDMAQDVHQLHAEVLFDGNRCPELNYARLVELGVEEKVDCQAWIKGDARHTSIAAASILAKVSRDKTMYAL
DAQHPEYGIAKHKGYPTRAHMEAIETYGVLAAHRRSFAPVRKALKR

Sequences:

>Translated_286_residues
MNSMLHIESNLLQIERSIEQIDIKGQYINLASPIYLSGQLNINELLAQYSNNIAQSDTSSVLQIGVDEAGRGPLLGSVNV
ASAILPRAWSGLIEGQPLKDTPLSILTDSKQLSEKKRDMLYPLVQQHAIGYVIADIPAAVIDQVNILQATMLGMRLCTEV
MLEVIAYHLNDMAQDVHQLHAEVLFDGNRCPELNYARLVELGVEEKVDCQAWIKGDARHTSIAAASILAKVSRDKTMYAL
DAQHPEYGIAKHKGYPTRAHMEAIETYGVLAAHRRSFAPVRKALKR
>Mature_286_residues
MNSMLHIESNLLQIERSIEQIDIKGQYINLASPIYLSGQLNINELLAQYSNNIAQSDTSSVLQIGVDEAGRGPLLGSVNV
ASAILPRAWSGLIEGQPLKDTPLSILTDSKQLSEKKRDMLYPLVQQHAIGYVIADIPAAVIDQVNILQATMLGMRLCTEV
MLEVIAYHLNDMAQDVHQLHAEVLFDGNRCPELNYARLVELGVEEKVDCQAWIKGDARHTSIAAASILAKVSRDKTMYAL
DAQHPEYGIAKHKGYPTRAHMEAIETYGVLAAHRRSFAPVRKALKR

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=223, Percent_Identity=40.8071748878924, Blast_Score=150, Evalue=9e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_PSYCK (Q1QAL9)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_580768.1
- ProteinModelPortal:   Q1QAL9
- SMR:   Q1QAL9
- STRING:   Q1QAL9
- GeneID:   4034264
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_1505
- NMPDR:   fig|335284.3.peg.1221
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   IICGVDE
- PhylomeDB:   Q1QAL9
- ProtClustDB:   CLSK839110
- BioCyc:   PCRY335284:PCRYO_1505-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 31647; Mature: 31647

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSMLHIESNLLQIERSIEQIDIKGQYINLASPIYLSGQLNINELLAQYSNNIAQSDTSS
CCCCEEHHHHHHHHHHHHHHHCCCCCEEECCCCEEEECCCCHHHHHHHHHCCHHHCCHHH
VLQIGVDEAGRGPLLGSVNVASAILPRAWSGLIEGQPLKDTPLSILTDSKQLSEKKRDML
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHEECCHHHHHHHHHHH
YPLVQQHAIGYVIADIPAAVIDQVNILQATMLGMRLCTEVMLEVIAYHLNDMAQDVHQLH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEVLFDGNRCPELNYARLVELGVEEKVDCQAWIKGDARHTSIAAASILAKVSRDKTMYAL
HHHHCCCCCCCCCCHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEE
DAQHPEYGIAKHKGYPTRAHMEAIETYGVLAAHRRSFAPVRKALKR
ECCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSMLHIESNLLQIERSIEQIDIKGQYINLASPIYLSGQLNINELLAQYSNNIAQSDTSS
CCCCEEHHHHHHHHHHHHHHHCCCCCEEECCCCEEEECCCCHHHHHHHHHCCHHHCCHHH
VLQIGVDEAGRGPLLGSVNVASAILPRAWSGLIEGQPLKDTPLSILTDSKQLSEKKRDML
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHEECCHHHHHHHHHHH
YPLVQQHAIGYVIADIPAAVIDQVNILQATMLGMRLCTEVMLEVIAYHLNDMAQDVHQLH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEVLFDGNRCPELNYARLVELGVEEKVDCQAWIKGDARHTSIAAASILAKVSRDKTMYAL
HHHHCCCCCCCCCCHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEE
DAQHPEYGIAKHKGYPTRAHMEAIETYGVLAAHRRSFAPVRKALKR
ECCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA