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The map label for this gene is miaA

Identifier: 93006320

GI number: 93006320

Start: 1837207

End: 1838451

Strand: Reverse

Name: miaA

Synonym: Pcryo_1494

Alternate gene names: 93006320

Gene position: 1838451-1837207 (Counterclockwise)

Preceding gene: 93006321

Following gene: 93006319

Centisome position: 60.08

GC content: 45.06

Gene sequence:

>1245_bases
ATGACCATCAAAGGCAAGTATATGCGACTTTCGTCTGATAGCCTATCTTCTTACTTAAGTCCCAATTTGGCGGACAATAG
CGTGGTGTGCTTGATGGCACCAACCGCGAGTGGTAAGACTGCTTTGGCTTACGAGCTTTATAATAGCGGTCGTTATGAGC
TGATTTCGGTAGATTCAGCATTGGTGTATCGTGACATGACGGTCGGTACCGCAAAACCAAACGCTGCTGAGCTGGCACGA
TACCCTCATCATTTGGTCGATATCATTGATCCTATGCAAAGCTATAGTGTCGCTGAGTTTGTCAACGATGTGGCAAGATT
GATTGATAGCTGTCATCAGAATGGCAAAATACCCTTGTTGGTCGGCGGTACGATGATGTATTACATGGCATTACTCGACG
GACTCTCACCAGTGCCTGATAGTGATGACAGTGTTCGCGCGCGCGTTGAGCAGTGGCGCCAAGATGAGGGTATCAGTGCG
CTATATGATTATCTTGGTAAAGTTGATGCTATCAGCCATGAGCGTCTTAATGCAACTGATACCCAGCGTATTACCCGTGC
TGTTGAAGTATATTTGCAAACTGATATTCCTATTAGTGACTGGCAGCGTCAGCCCAAGCAAGCATTGGCATATAATCCCA
ATCAGCAATGGCATGCTTTGGCGGTTATGCCAGATCGTCCATGGTTGCATACGCGTATTGAGCAGCGATTAGATATCATG
TGGCGTGAAGGGTTAGTAGCAGAGGTTATCAGTTTGCTTGAGCATTATCCTTTGACCCCAGGCTTACCTTCGATGCGCTG
TGTTGGTTATCGACAAGTATTAGAGTATTTGGTACAGACTAATCACCCTGTATTTGAACAGCCTCATTTGGATAAAGCGC
AGTTTTATGATGCTTTTGCTCAATCGGAACCATCAAGTAACGAAAGTAAAGAGCAAGATACCACGATTCAAAGTAGCATA
ACTCAGACGCATCTACCAATGGCAACTGCCCAAACCGAAGCGCTTGCTTGTCAGCAAATGCAAAATAAGGCATTATATGC
TACAAGGCAGTTAGCAAAGCGCCAATACACGTGGCTGCGAAAAGTGATGCAACTGTCTAATACGGCAGCAGTGCCTACAA
CAGAATCAATAACAGCGTCGTCGACATCCACTGTTGGCTCTGATGATGTGACAGATTTTCAAAAAAATAAGTTAATATTG
CACTCATTTAGCACTATGGAGCAGGCGAGAGCGTATTTGGTTTAA

Upstream 100 bases:

>100_bases
TCATCTGCGCTTAAATAGCGCTATTGTGAAAATAACGTATTTTCTATAGTTAGGACATATCGCTGAGCTATCAATAATTT
ACCTTATTGTTCTTAAAACC

Downstream 100 bases:

>100_bases
TGAATGATATCAGTCGTTTGCCTATATCTAGATTTGCCAGACCATTAGTGTTCTGTAATATCAATGGTCGAGCTGTAGTA
ATAAAAACACAGCATGACCG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MTIKGKYMRLSSDSLSSYLSPNLADNSVVCLMAPTASGKTALAYELYNSGRYELISVDSALVYRDMTVGTAKPNAAELAR
YPHHLVDIIDPMQSYSVAEFVNDVARLIDSCHQNGKIPLLVGGTMMYYMALLDGLSPVPDSDDSVRARVEQWRQDEGISA
LYDYLGKVDAISHERLNATDTQRITRAVEVYLQTDIPISDWQRQPKQALAYNPNQQWHALAVMPDRPWLHTRIEQRLDIM
WREGLVAEVISLLEHYPLTPGLPSMRCVGYRQVLEYLVQTNHPVFEQPHLDKAQFYDAFAQSEPSSNESKEQDTTIQSSI
TQTHLPMATAQTEALACQQMQNKALYATRQLAKRQYTWLRKVMQLSNTAAVPTTESITASSTSTVGSDDVTDFQKNKLIL
HSFSTMEQARAYLV

Sequences:

>Translated_414_residues
MTIKGKYMRLSSDSLSSYLSPNLADNSVVCLMAPTASGKTALAYELYNSGRYELISVDSALVYRDMTVGTAKPNAAELAR
YPHHLVDIIDPMQSYSVAEFVNDVARLIDSCHQNGKIPLLVGGTMMYYMALLDGLSPVPDSDDSVRARVEQWRQDEGISA
LYDYLGKVDAISHERLNATDTQRITRAVEVYLQTDIPISDWQRQPKQALAYNPNQQWHALAVMPDRPWLHTRIEQRLDIM
WREGLVAEVISLLEHYPLTPGLPSMRCVGYRQVLEYLVQTNHPVFEQPHLDKAQFYDAFAQSEPSSNESKEQDTTIQSSI
TQTHLPMATAQTEALACQQMQNKALYATRQLAKRQYTWLRKVMQLSNTAAVPTTESITASSTSTVGSDDVTDFQKNKLIL
HSFSTMEQARAYLV
>Mature_413_residues
TIKGKYMRLSSDSLSSYLSPNLADNSVVCLMAPTASGKTALAYELYNSGRYELISVDSALVYRDMTVGTAKPNAAELARY
PHHLVDIIDPMQSYSVAEFVNDVARLIDSCHQNGKIPLLVGGTMMYYMALLDGLSPVPDSDDSVRARVEQWRQDEGISAL
YDYLGKVDAISHERLNATDTQRITRAVEVYLQTDIPISDWQRQPKQALAYNPNQQWHALAVMPDRPWLHTRIEQRLDIMW
REGLVAEVISLLEHYPLTPGLPSMRCVGYRQVLEYLVQTNHPVFEQPHLDKAQFYDAFAQSEPSSNESKEQDTTIQSSIT
QTHLPMATAQTEALACQQMQNKALYATRQLAKRQYTWLRKVMQLSNTAAVPTTESITASSTSTVGSDDVTDFQKNKLILH
SFSTMEQARAYLV

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=285, Percent_Identity=26.3157894736842, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1790613, Length=333, Percent_Identity=41.1411411411411, Blast_Score=223, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI25144712, Length=281, Percent_Identity=26.3345195729537, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324848, Length=257, Percent_Identity=24.9027237354086, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24649830, Length=400, Percent_Identity=24, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_PSYCK (Q1QAN0)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_580757.1
- ProteinModelPortal:   Q1QAN0
- SMR:   Q1QAN0
- STRING:   Q1QAN0
- GeneID:   4034462
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_1494
- NMPDR:   fig|335284.3.peg.1220
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   DAISHER
- PhylomeDB:   Q1QAN0
- ProtClustDB:   CLSK839114
- BioCyc:   PCRY335284:PCRYO_1494-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 46624; Mature: 46493

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKGKYMRLSSDSLSSYLSPNLADNSVVCLMAPTASGKTALAYELYNSGRYELISVDSA
CCCCCCEEEECCHHHHHHCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEECCCH
LVYRDMTVGTAKPNAAELARYPHHLVDIIDPMQSYSVAEFVNDVARLIDSCHQNGKIPLL
HHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
VGGTMMYYMALLDGLSPVPDSDDSVRARVEQWRQDEGISALYDYLGKVDAISHERLNATD
ECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHH
TQRITRAVEVYLQTDIPISDWQRQPKQALAYNPNQQWHALAVMPDRPWLHTRIEQRLDIM
HHHHHHHHHHHHCCCCCHHHHHCCCHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHH
WREGLVAEVISLLEHYPLTPGLPSMRCVGYRQVLEYLVQTNHPVFEQPHLDKAQFYDAFA
HHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
QSEPSSNESKEQDTTIQSSITQTHLPMATAQTEALACQQMQNKALYATRQLAKRQYTWLR
CCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVMQLSNTAAVPTTESITASSTSTVGSDDVTDFQKNKLILHSFSTMEQARAYLV
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCEEEEEHHHHHHHHHHHCC
>Mature Secondary Structure 
TIKGKYMRLSSDSLSSYLSPNLADNSVVCLMAPTASGKTALAYELYNSGRYELISVDSA
CCCCCEEEECCHHHHHHCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEECCCH
LVYRDMTVGTAKPNAAELARYPHHLVDIIDPMQSYSVAEFVNDVARLIDSCHQNGKIPLL
HHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
VGGTMMYYMALLDGLSPVPDSDDSVRARVEQWRQDEGISALYDYLGKVDAISHERLNATD
ECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHH
TQRITRAVEVYLQTDIPISDWQRQPKQALAYNPNQQWHALAVMPDRPWLHTRIEQRLDIM
HHHHHHHHHHHHCCCCCHHHHHCCCHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHH
WREGLVAEVISLLEHYPLTPGLPSMRCVGYRQVLEYLVQTNHPVFEQPHLDKAQFYDAFA
HHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
QSEPSSNESKEQDTTIQSSITQTHLPMATAQTEALACQQMQNKALYATRQLAKRQYTWLR
CCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVMQLSNTAAVPTTESITASSTSTVGSDDVTDFQKNKLILHSFSTMEQARAYLV
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCEEEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA