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The map label for this gene is murB

Identifier: 93006296

GI number: 93006296

Start: 1810605

End: 1811726

Strand: Direct

Name: murB

Synonym: Pcryo_1470

Alternate gene names: 93006296

Gene position: 1810605-1811726 (Clockwise)

Preceding gene: 93006295

Following gene: 93006297

Centisome position: 59.17

GC content: 46.52

Gene sequence:

>1122_bases
ATGACTTCAGCTTTATGCTCTAAACCCATTGCACCACATACTCTATCTGATGACTTAGCTGATTTGTCTTATAGCAATAC
TATGGCTTTGGCATGTATGGCTGACTCTGTAGTTACCTTAACGGATGAGGTGCAACTTGATGAGTTTATGGCATATTATG
AGCAAGATACTCAACATAGAAAGCCATTATTTGTCTTGTCCGGTGGTAGCAATGTGCTACTACCAGCAAAGCTCAATGCG
ATTGTCTTACGACCACAAATGCGTGGTATACAGGTGACAGCGCAAACAGACTTTCATGTCGATATCGAGGTAATGGCAGG
TGAAAACTGGCACGACTTGGTGGTACATACGGTCGCTCAAGGCTGGTATGGACTCGAAAACCTTGCACTCATACCTGGTC
TTACGGGCGCCGCTCCCATACAAAATATTGGCGCTTATGGTGTTCAGCTAGAAGACTGCTTACAGTATGTACGTGCTTAC
CATCTGCCAAGTCAAACGTGGCATGATTTGACCGCGGTCGACTGCGAGTTTGGCTACCGTGATAGTATATTTAAACGTCA
GCCGAATACGTGGCTAATCAGCCGTGTGGGTTTTAGATTGCATACTGATGCGACAAAGGTGCTGGCAAGCTATGGCGATG
TACAGACCGTCGCACAGAGCTATGCGACACAGCAAGGTCGCACCAAACCGATGCCCGCTGACGTAATGCATGCCATCATT
GAGATTCGTCAGCAAAAGCTGCCTGACCCGAAGCAATTACCAAATTGCGGCAGTTTTTTTCAAAATCCTATTGTCCCTCA
AGATCAATTTGCTACGCTGCAATCATCTTATCCGGCTATCGTTGGCTACCCAATGCCTGACGCAATGACTAAAGTGGCAG
CCGGCTGGCTTATCGAACAAGCGGGTCTAAAAGGCGGCGGTATAGAGCCTATTTTTACCCATCAGCAGCAAGCGCTAGTA
TTGACCAATCACGCGCCGTATATTGCTACCAAACAAGATGTCGCCGCTGCTCAAAAATATATTATAGATACTGTCTATAA
GAAATTTGCGATTCAACTGTCGCGTGAGCCAGTATGGGTAAATGCCGATGGCTCCATTGGATACGATGAGCATGTGGTCT
AA

Upstream 100 bases:

>100_bases
TAGTAATCATAAGGCGCTATATTTTTGCTACCTTTATACCTAGCATCCTTTATAATAAATACCATATGACTTTTTGTTTA
CTTGCCCCATAGGCTATGTT

Downstream 100 bases:

>100_bases
GCAGTCATGGTCTAATCGCCTATGGCGACATTATTTACGTTCAGCTGAGCGTATGATCAAGCTGTTTCGTATTATCAATG
TCACGTTTTTGCTGGGTGCA

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MTSALCSKPIAPHTLSDDLADLSYSNTMALACMADSVVTLTDEVQLDEFMAYYEQDTQHRKPLFVLSGGSNVLLPAKLNA
IVLRPQMRGIQVTAQTDFHVDIEVMAGENWHDLVVHTVAQGWYGLENLALIPGLTGAAPIQNIGAYGVQLEDCLQYVRAY
HLPSQTWHDLTAVDCEFGYRDSIFKRQPNTWLISRVGFRLHTDATKVLASYGDVQTVAQSYATQQGRTKPMPADVMHAII
EIRQQKLPDPKQLPNCGSFFQNPIVPQDQFATLQSSYPAIVGYPMPDAMTKVAAGWLIEQAGLKGGGIEPIFTHQQQALV
LTNHAPYIATKQDVAAAQKYIIDTVYKKFAIQLSREPVWVNADGSIGYDEHVV

Sequences:

>Translated_373_residues
MTSALCSKPIAPHTLSDDLADLSYSNTMALACMADSVVTLTDEVQLDEFMAYYEQDTQHRKPLFVLSGGSNVLLPAKLNA
IVLRPQMRGIQVTAQTDFHVDIEVMAGENWHDLVVHTVAQGWYGLENLALIPGLTGAAPIQNIGAYGVQLEDCLQYVRAY
HLPSQTWHDLTAVDCEFGYRDSIFKRQPNTWLISRVGFRLHTDATKVLASYGDVQTVAQSYATQQGRTKPMPADVMHAII
EIRQQKLPDPKQLPNCGSFFQNPIVPQDQFATLQSSYPAIVGYPMPDAMTKVAAGWLIEQAGLKGGGIEPIFTHQQQALV
LTNHAPYIATKQDVAAAQKYIIDTVYKKFAIQLSREPVWVNADGSIGYDEHVV
>Mature_372_residues
TSALCSKPIAPHTLSDDLADLSYSNTMALACMADSVVTLTDEVQLDEFMAYYEQDTQHRKPLFVLSGGSNVLLPAKLNAI
VLRPQMRGIQVTAQTDFHVDIEVMAGENWHDLVVHTVAQGWYGLENLALIPGLTGAAPIQNIGAYGVQLEDCLQYVRAYH
LPSQTWHDLTAVDCEFGYRDSIFKRQPNTWLISRVGFRLHTDATKVLASYGDVQTVAQSYATQQGRTKPMPADVMHAIIE
IRQQKLPDPKQLPNCGSFFQNPIVPQDQFATLQSSYPAIVGYPMPDAMTKVAAGWLIEQAGLKGGGIEPIFTHQQQALVL
TNHAPYIATKQDVAAAQKYIIDTVYKKFAIQLSREPVWVNADGSIGYDEHVV

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1790407, Length=348, Percent_Identity=39.0804597701149, Blast_Score=216, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_PSYCK (Q1QAQ4)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_580733.1
- ProteinModelPortal:   Q1QAQ4
- SMR:   Q1QAQ4
- STRING:   Q1QAQ4
- GeneID:   4035116
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_1470
- NMPDR:   fig|335284.3.peg.1364
- eggNOG:   COG0812
- HOGENOM:   HBG686573
- OMA:   GAAPMQN
- PhylomeDB:   Q1QAQ4
- ProtClustDB:   PRK00046
- BioCyc:   PCRY335284:PCRYO_1470-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 41232; Mature: 41101

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 180-180 ACT_SITE 258-258 ACT_SITE 356-356

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSALCSKPIAPHTLSDDLADLSYSNTMALACMADSVVTLTDEVQLDEFMAYYEQDTQHR
CCCHHHCCCCCCCCCCCHHHHCCCCCCEEEEEECCCEEEEECHHHHHHHHHHHHHHHCCC
KPLFVLSGGSNVLLPAKLNAIVLRPQMRGIQVTAQTDFHVDIEVMAGENWHDLVVHTVAQ
CCEEEEECCCCEEEEECCCEEEECCCCCCEEEEEECCEEEEEEEEECCCHHHHHHHHHHH
GWYGLENLALIPGLTGAAPIQNIGAYGVQLEDCLQYVRAYHLPSQTWHDLTAVDCEFGYR
CCCCCCCEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCC
DSIFKRQPNTWLISRVGFRLHTDATKVLASYGDVQTVAQSYATQQGRTKPMPADVMHAII
CHHHHCCCCEEEEEECCEEEECHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
EIRQQKLPDPKQLPNCGSFFQNPIVPQDQFATLQSSYPAIVGYPMPDAMTKVAAGWLIEQ
HHHHHHCCCHHHCCCCHHHHCCCCCCCHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHH
AGLKGGGIEPIFTHQQQALVLTNHAPYIATKQDVAAAQKYIIDTVYKKFAIQLSREPVWV
CCCCCCCCCCEEECCCCEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHEECCCCEEE
NADGSIGYDEHVV
ECCCCCCCCCCCC
>Mature Secondary Structure 
TSALCSKPIAPHTLSDDLADLSYSNTMALACMADSVVTLTDEVQLDEFMAYYEQDTQHR
CCHHHCCCCCCCCCCCHHHHCCCCCCEEEEEECCCEEEEECHHHHHHHHHHHHHHHCCC
KPLFVLSGGSNVLLPAKLNAIVLRPQMRGIQVTAQTDFHVDIEVMAGENWHDLVVHTVAQ
CCEEEEECCCCEEEEECCCEEEECCCCCCEEEEEECCEEEEEEEEECCCHHHHHHHHHHH
GWYGLENLALIPGLTGAAPIQNIGAYGVQLEDCLQYVRAYHLPSQTWHDLTAVDCEFGYR
CCCCCCCEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCC
DSIFKRQPNTWLISRVGFRLHTDATKVLASYGDVQTVAQSYATQQGRTKPMPADVMHAII
CHHHHCCCCEEEEEECCEEEECHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
EIRQQKLPDPKQLPNCGSFFQNPIVPQDQFATLQSSYPAIVGYPMPDAMTKVAAGWLIEQ
HHHHHHCCCHHHCCCCHHHHCCCCCCCHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHH
AGLKGGGIEPIFTHQQQALVLTNHAPYIATKQDVAAAQKYIIDTVYKKFAIQLSREPVWV
CCCCCCCCCCEEECCCCEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHEECCCCEEE
NADGSIGYDEHVV
ECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA