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The map label for this gene is arsB [H]

Identifier: 93006289

GI number: 93006289

Start: 1804233

End: 1805537

Strand: Reverse

Name: arsB [H]

Synonym: Pcryo_1463

Alternate gene names: 93006289

Gene position: 1805537-1804233 (Counterclockwise)

Preceding gene: 93006290

Following gene: 93006288

Centisome position: 59.01

GC content: 45.29

Gene sequence:

>1305_bases
ATGCTCGCATTGATTATTTTTATCGTTACTTTAGGGCTAGTAATATGGCAACCTAAAGGTTTGGGCATTGGTTGGAGTGC
GATGGGCGGGGCGTTGGTCGCCCTAGCTTTTGGTGTGGTGCAATTATCTGATGTCGGTGTCGTGTGGGACATCGTTTGGG
ATGCTACCTTTACCTTTGTCGCCCTGATTATCATCTCTCTTATCTTAGATAAGGCTGGGTTTTTTGCTTGGGCAGCATTG
CATGTAGCGAGATTGGGTAATGGACAAGGGCGTCTATTGTTCCCTATGATTGTGATATTAGGCGCTTTTATTTCTGCGTT
TTTTGCCAATGATGGGGCGGTATTATTACTGACCCCGATTGTGATTGCGATTTTATTACGGTTAAATTTTTCGCCGCCAT
CTGCATTGGCATTTATCATAGCGACTGGTTTTATTGCTGATACTGCAAGTTTGCCATTGGTCACATCTAACCTCGTTAAT
ATCGTTAGTGCTAACTACTTTGATATTGGTTTTGGACGTTATGCTGCGGTTATGGTACCGGTCAATATTGTCTCTGTACT
AGCGACGCTAGTGGTGCTATGGATATTTTATGCATGCCACATTCCAACACACTACTCGATGGCGGACCTTAGTGCACCTG
AGTCAGCGATCGAAGACACCTTGGTTTTTAAAGCTGCCTTTCCGCTACTGGCTTTGTTGCTTGTCGCTTATTTTGCGACA
GAGTCGCTTGGTATTCCTATCTCGCTCGTTACTGGCGCAGCTGCATTACTGCTCATGGCGATTGCAGGGCGATTTTGGCA
AGGCGGTCGTGGGGCGGTGATGTCCGTGCCTGAAATTGTCCGTAAAGCACCTTGGCAGATTGTTTTATTTTCTATCGGTA
TGTACTTGGTTGTTTACGGTTTGGGCAATGCAGGATTGACTGCCTACGGCGCTCAAATCTTGAATTGGTTAGGGCAACAA
GGAACCCTAATAGCAACCTTGGGCACAGGATTCCTCTCCGCTATCGTGGCATCTATTATGAATAACATGCCCTCTACCTT
GATAGGCGCACTAGCGATTGATAGCGCCCAAGTACCGACCGCCACTCGTGAGCTGATGATTTATGCCAATGTTATTGGCA
ATGATTTAGGACCCAAATTTACGCCTATGGGCAGTCTTGCGACTTTACTATGGCTGCATGTATTGGCAGAAAAAGATTAT
AAAATCAGCTGGGGGCAGTATATGAAAATTGGTTTGATTATCACCCCGCCAGTGTTATTAGTCACCTTATTAGCTTTAGT
ATTTTGGTTGCCCTATTTGTCTTAG

Upstream 100 bases:

>100_bases
TTATTCAATAAAATAGGCTAAATCAAGTAGGCTTCATGAAGCCTACTTGAATAAATGTCACTAAGTTTTCTTAAGTAAGT
CTGCTTAATTAAGGTTGCTC

Downstream 100 bases:

>100_bases
TCGTAAAAATTAACGTCATATCTTATTATTAGAGGTGCTATTACGCTGACATCCATCAAATTCATCGGTTATAATAGCGG
CGCATTTTACCTCTTTTACC

Product: arsenical pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MLALIIFIVTLGLVIWQPKGLGIGWSAMGGALVALAFGVVQLSDVGVVWDIVWDATFTFVALIIISLILDKAGFFAWAAL
HVARLGNGQGRLLFPMIVILGAFISAFFANDGAVLLLTPIVIAILLRLNFSPPSALAFIIATGFIADTASLPLVTSNLVN
IVSANYFDIGFGRYAAVMVPVNIVSVLATLVVLWIFYACHIPTHYSMADLSAPESAIEDTLVFKAAFPLLALLLVAYFAT
ESLGIPISLVTGAAALLLMAIAGRFWQGGRGAVMSVPEIVRKAPWQIVLFSIGMYLVVYGLGNAGLTAYGAQILNWLGQQ
GTLIATLGTGFLSAIVASIMNNMPSTLIGALAIDSAQVPTATRELMIYANVIGNDLGPKFTPMGSLATLLWLHVLAEKDY
KISWGQYMKIGLIITPPVLLVTLLALVFWLPYLS

Sequences:

>Translated_434_residues
MLALIIFIVTLGLVIWQPKGLGIGWSAMGGALVALAFGVVQLSDVGVVWDIVWDATFTFVALIIISLILDKAGFFAWAAL
HVARLGNGQGRLLFPMIVILGAFISAFFANDGAVLLLTPIVIAILLRLNFSPPSALAFIIATGFIADTASLPLVTSNLVN
IVSANYFDIGFGRYAAVMVPVNIVSVLATLVVLWIFYACHIPTHYSMADLSAPESAIEDTLVFKAAFPLLALLLVAYFAT
ESLGIPISLVTGAAALLLMAIAGRFWQGGRGAVMSVPEIVRKAPWQIVLFSIGMYLVVYGLGNAGLTAYGAQILNWLGQQ
GTLIATLGTGFLSAIVASIMNNMPSTLIGALAIDSAQVPTATRELMIYANVIGNDLGPKFTPMGSLATLLWLHVLAEKDY
KISWGQYMKIGLIITPPVLLVTLLALVFWLPYLS
>Mature_434_residues
MLALIIFIVTLGLVIWQPKGLGIGWSAMGGALVALAFGVVQLSDVGVVWDIVWDATFTFVALIIISLILDKAGFFAWAAL
HVARLGNGQGRLLFPMIVILGAFISAFFANDGAVLLLTPIVIAILLRLNFSPPSALAFIIATGFIADTASLPLVTSNLVN
IVSANYFDIGFGRYAAVMVPVNIVSVLATLVVLWIFYACHIPTHYSMADLSAPESAIEDTLVFKAAFPLLALLLVAYFAT
ESLGIPISLVTGAAALLLMAIAGRFWQGGRGAVMSVPEIVRKAPWQIVLFSIGMYLVVYGLGNAGLTAYGAQILNWLGQQ
GTLIATLGTGFLSAIVASIMNNMPSTLIGALAIDSAQVPTATRELMIYANVIGNDLGPKFTPMGSLATLLWLHVLAEKDY
KISWGQYMKIGLIITPPVLLVTLLALVFWLPYLS

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082277, Length=432, Percent_Identity=58.5648148148148, Blast_Score=469, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 46215; Mature: 46215

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLALIIFIVTLGLVIWQPKGLGIGWSAMGGALVALAFGVVQLSDVGVVWDIVWDATFTFV
CHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ALIIISLILDKAGFFAWAALHVARLGNGQGRLLFPMIVILGAFISAFFANDGAVLLLTPI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
VIAILLRLNFSPPSALAFIIATGFIADTASLPLVTSNLVNIVSANYFDIGFGRYAAVMVP
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHH
VNIVSVLATLVVLWIFYACHIPTHYSMADLSAPESAIEDTLVFKAAFPLLALLLVAYFAT
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESLGIPISLVTGAAALLLMAIAGRFWQGGRGAVMSVPEIVRKAPWQIVLFSIGMYLVVYG
HCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHC
LGNAGLTAYGAQILNWLGQQGTLIATLGTGFLSAIVASIMNNMPSTLIGALAIDSAQVPT
CCCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCC
ATRELMIYANVIGNDLGPKFTPMGSLATLLWLHVLAEKDYKISWGQYMKIGLIITPPVLL
HHHHHHEEHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCEEEEEEEECCHHHH
VTLLALVFWLPYLS
HHHHHHHHHHHCCC
>Mature Secondary Structure
MLALIIFIVTLGLVIWQPKGLGIGWSAMGGALVALAFGVVQLSDVGVVWDIVWDATFTFV
CHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ALIIISLILDKAGFFAWAALHVARLGNGQGRLLFPMIVILGAFISAFFANDGAVLLLTPI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
VIAILLRLNFSPPSALAFIIATGFIADTASLPLVTSNLVNIVSANYFDIGFGRYAAVMVP
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHH
VNIVSVLATLVVLWIFYACHIPTHYSMADLSAPESAIEDTLVFKAAFPLLALLLVAYFAT
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESLGIPISLVTGAAALLLMAIAGRFWQGGRGAVMSVPEIVRKAPWQIVLFSIGMYLVVYG
HCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHC
LGNAGLTAYGAQILNWLGQQGTLIATLGTGFLSAIVASIMNNMPSTLIGALAIDSAQVPT
CCCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCC
ATRELMIYANVIGNDLGPKFTPMGSLATLLWLHVLAEKDYKISWGQYMKIGLIITPPVLL
HHHHHHEEHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCEEEEEEEECCHHHH
VTLLALVFWLPYLS
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3021763; 2523997; 1688427; 1704144 [H]